ChtrB.00177.a

Chaperone protein DnaK (75 kDa membrane protein) (HSP70) (Heat shock 70 kDa protein) (Heat shock protein 70)

CENTER ID:	ChtrB.00177.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.00177.a.B1.GE42403	full length		1	660

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.00177.a.B1.PW38506	full length		1	660

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_82027

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|272561.5.peg.426
UniProt:	P17821

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSEKRKSNKI IGIDLGTTNS CVSVMEGGQP KVIASSEGTR TTPSIVAFKG GETLVGIPAK RQAVTNPEKT LASTKRFIGR KFSEVESEIK TVPYKVAPNS KGDAVFDVEQ KLYTPEEIGA QILMKMKETA EAYLGETVTE AVITVPAYFN DSQRASTKDA GRIAGLDVKR IIPEPTAAAL AYGIDKEGDK KIAVFDLGGG TFDISILEIG DGVFEVLSTN GDTHLGGDDF DGVIINWMLD EFKKQEGIDL SKDNMALQRL KDAAEKAKIE LSGVSSTEIN QPFITIDANG PKHLALTLTR AQFEHLASSL IERTKQPCAQ ALKDAKLSAS DIDDVLLVGG MSRMPAVQAV VKEIFGKEPN KGVNPDEVVA IGAAIQGGVL GGEVKDVLLL DVIPLSLGIE TLGGVMTPLV ERNTTIPTQK KQIFSTAADN QPAVTIVVLQ GERPMAKDNK EIGRFDLTDI PPAPRGHPQI EVTFDIDANG ILHVSAKDAA SGREQKIRIE ASSGLKEDEI QQMIRDAELH KEEDKQRKEA SDVKNEADGM IFRAEKAVKD YHDKIPAELV KEIEEHIEKV RQAIKEDAST TAIKAASDEL STHMQKIGEA MQAQSASAAA SSAANAQGGP NINSEDLKKH SFSTRPPAGG SASSTDNIED ADVEIVDKPE

NT Sequence

atgagcgaaa aaagaaagtc taacaaaatt attggtatcg acctagggac gaccaactct tgcgtctctg ttatggaagg tggccaacct aaagttattg cctcttctga aggaactcgt actactcctt ctatcgttgc ttttaaaggt ggcgaaactc ttgttggaat tcctgcaaaa cgtcaggcag taaccaatcc tgaaaaaaca ttggcttcta ctaagcgatt catcggtaga aaattctctg aagtcgaatc tgaaattaaa acagtcccct acaaagttgc tcctaactcg aaaggagatg cggtctttga tgtggaacaa aaactgtaca ctccagaaga aatcggcgct cagatcctca tgaagatgaa ggaaactgct gaggcttatc tcggagaaac agtaacggaa gcagtcatta ccgtaccagc ttactttaac gattctcaaa gagcttctac aaaagatgct ggacgtatcg caggattaga tgttaaacgc attattcctg aaccaacagc ggccgctctt gcttatggta ttgataagga aggagataaa aaaatcgccg tcttcgactt aggaggagga actttcgata tttctatctt ggaaatcggt gacggagttt ttgaagttct ctcaaccaac ggggatactc acttgggagg agacgacttc gatggagtca tcatcaactg gatgcttgat gaattcaaaa aacaagaagg cattgatcta agcaaagata acatggcttt gcaaagattg aaagatgctg ctgaaaaagc aaaaatagaa ttgtctggtg tatcgtctac tgaaatcaat cagccattca tcactatcga cgctaatgga cctaaacatt tggctttaac tctaactcgc gctcaattcg aacacctagc ttcctctctc attgagcgaa ccaaacaacc ttgtgctcag gctttaaaag atgctaaatt gtccgcttct gacattgatg atgttcttct agttggcgga atgtccagaa tgcctgcggt acaagcagtt gtaaaagaga tctttggtaa agagcctaat aaaggcgtca atccagatga agttgtagcg attggagctg ctattcaggg tggtgtcctc ggcggagaag tgaaagacgt tctgttgttg gatgtgattc ccctctcttt aggaattgag actctaggtg gggtcatgac tcctttggta gagagaaaca ctacaatccc tactcagaag aagcaaatct tctctacagc cgctgacaat cagccagcag tgactatcgt cgttcttcaa ggtgaacggc ctatggcgaa agacaataag gaaattggaa gatttgatct aacagacatt cctcctgctc ctcgcggcca tccacaaatt gaggtaacct tcgatattga tgccaacgga attttacacg tttctgctaa agatgctgct agtggacgcg aacaaaaaat ccgtattgaa gcaagctctg gattaaaaga agatgaaatt caacaaatga tccgcgatgc agagcttcat aaagaggaag acaaacaacg aaaagaagct tctgatgtga aaaatgaagc cgatggaatg atctttagag ccgaaaaagc tgtgaaagat taccacgaca aaattcctgc agaacttgtt aaagaaattg aagagcatat tgagaaagta cgccaagcaa tcaaagaaga tgcttccaca acagctatca aagcagcttc tgatgagttg agtactcata tgcaaaaaat cggagaagct atgcaggctc aatccgcatc cgcagcagca tcttctgcag cgaatgctca aggagggcca aacattaact ccgaagatct gaaaaaacat agtttcagca cacgacctcc agcaggagga agcgcctctt ctacagacaa cattgaagat gctgatgttg aaattgttga taaacctgag taa

Details for ChtrB.00177.a.B1.GE42403

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	72kDa
OBSERVED MW:	72kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS EKRKSNKIIG IDLGTTNSCV SVMEGGQPKV IASSEGTRTT PSIVAFKGGE TLVGIPAKRQ AVTNPEKTLA STKRFIGRKF SEVESEIKTV PYKVAPNSKG DAVFDVEQKL YTPEEIGAQI LMKMKETAEA YLGETVTEAV ITVPAYFNDS QRASTKDAGR IAGLDVKRII PEPTAAALAY GIDKEGDKKI AVFDLGGGTF DISILEIGDG VFEVLSTNGD THLGGDDFDG VIINWMLDEF KKQEGIDLSK DNMALQRLKD AAEKAKIELS GVSSTEINQP FITIDANGPK HLALTLTRAQ FEHLASSLIE RTKQPCAQAL KDAKLSASDI DDVLLVGGMS RMPAVQAVVK EIFGKEPNKG VNPDEVVAIG AAIQGGVLGG EVKDVLLLDV IPLSLGIETL GGVMTPLVER NTTIPTQKKQ IFSTAADNQP AVTIVVLQGE RPMAKDNKEI GRFDLTDIPP APRGHPQIEV TFDIDANGIL HVSAKDAASG REQKIRIEAS SGLKEDEIQQ MIRDAELHKE EDKQRKEASD VKNEADGMIF RAEKAVKDYH DKIPAELVKE IEEHIEKVRQ AIKEDASTTA IKAASDELST HMQKIGEAMQ AQSASAAASS AANAQGGPNI NSEDLKKHSF STRPPAGGSA SSTDNIEDAD VEIVDKPE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg aaaaaagaaa gtctaacaaa attattggta tcgacctagg gacgaccaac tcttgcgtct ctgttatgga aggtggccaa cctaaagtta ttgcctcttc tgaaggaact cgtactactc cttctatcgt tgcttttaaa ggtggcgaaa ctcttgttgg aattcctgca aaacgtcagg cagtaaccaa tcctgaaaaa acattggctt ctactaagcg attcatcggt agaaaattct ctgaagtcga atctgaaatt aaaacagtcc cctacaaagt tgctcctaac tcgaaaggag atgcggtctt tgatgtggaa caaaaactgt acactccaga agaaatcggc gctcagatcc tcatgaagat gaaggaaact gctgaggctt atctcggaga aacagtaacg gaagcagtca ttaccgtacc agcttacttt aacgattctc aaagagcttc tacaaaagat gctggacgta tcgcaggatt agatgttaaa cgcattattc ctgaaccaac agcggccgct cttgcttatg gtattgataa ggaaggagat aaaaaaatcg ccgtcttcga cttaggagga ggaactttcg atatttctat cttggaaatc ggtgacggag tttttgaagt tctctcaacc aacggggata ctcacttggg aggagacgac ttcgatggag tcatcatcaa ctggatgctt gatgaattca aaaaacaaga aggcattgat ctaagcaaag ataacatggc tttgcaaaga ttgaaagatg ctgctgaaaa agcaaaaata gaattgtctg gtgtatcgtc tactgaaatc aatcagccat tcatcactat cgacgctaat ggacctaaac atttggcttt aactctaact cgcgctcaat tcgaacacct agcttcctct ctcattgagc gaaccaaaca accttgtgct caggctttaa aagatgctaa attgtccgct tctgacattg atgatgttct tctagttggc ggaatgtcca gaatgcctgc ggtacaagca gttgtaaaag agatctttgg taaagagcct aataaaggcg tcaatccaga tgaagttgta gcgattggag ctgctattca gggtggtgtc ctcggcggag aagtgaaaga cgttctgttg ttggatgtga ttcccctctc tttaggaatt gagactctag gtggggtcat gactcctttg gtagagagaa acactacaat ccctactcag aagaagcaaa tcttctctac agccgctgac aatcagccag cagtgactat cgtcgttctt caaggtgaac ggcctatggc gaaagacaat aaggaaattg gaagatttga tctaacagac attcctcctg ctcctcgcgg ccatccacaa attgaggtaa ccttcgatat tgatgccaac ggaattttac acgtttctgc taaagatgct gctagtggac gcgaacaaaa aatccgtatt gaagcaagct ctggattaaa agaagatgaa attcaacaaa tgatccgcga tgcagagctt cataaagagg aagacaaaca acgaaaagaa gcttctgatg tgaaaaatga agccgatgga atgatcttta gagccgaaaa agctgtgaaa gattaccacg acaaaattcc tgcagaactt gttaaagaaa ttgaagagca tattgagaaa gtacgccaag caatcaaaga agatgcttcc acaacagcta tcaaagcagc ttctgatgag ttgagtactc atatgcaaaa aatcggagaa gctatgcagg ctcaatccgc atccgcagca gcatcttctg cagcgaatgc tcaaggaggg ccaaacatta actccgaaga tctgaaaaaa catagtttca gcacacgacc tccagcagga ggaagcgcct cttctacaga caacattgaa gatgctgatg ttgaaattgt tgataaacct gagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for ChtrB.00177.a.B1.PW38506

PURIFICATION DATe:	7/9/2018
CONCENTRATION:	18.41mg/ml
OBSERVED MW:	75kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	18
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG AAAAAAGAAA GTCTAACAAA ATTATTGGTA TCGACCTAGG GACGACCAAC TCTTGCGTCT CTGTTATGGA AGGTGGCCAA CCTAAAGTTA TTGCCTCTTC TGAAGGAACT CGTACTACTC CTTCTATCGT TGCTTTTAAA GGTGGCGAAA CTCTTGTTGG AATTCCTGCA AAACGTCAGG CAGTAACCAA TCCTGAAAAA ACATTGGCTT CTACTAAGCG ATTCATCGGT AGAAAATTCT CTGAAGTCGA ATCTGAAATT AAAACAGTCC CCTACAAAGT TGCTCCTAAC TCGAAAGGAG ATGCGGTCTT TGATGTGGAA CAAAAACTGT ACACTCCAGA AGAAATCGGC GCTCAGATCC TCATGAAGAT GAAGGAAACT GCTGAGGCTT ATCTCGGAGA AACAGTAACG GAAGCAGTCA TTACCGTACC AGCTTACTTT AACGATTCTC AAAGAGCTTC TACAAAAGAT GCTGGACGTA TCGCAGGATT AGATGTTAAA CGCATTATTC CTGAACCAAC AGCGGCCGCT CTTGCTTATG GTATTGATAA GGAAGGAGAT AAAAAAATCG CCGTCTTCGA CTTAGGAGGA GGAACTTTCG ATATTTCTAT CTTGGAAATC GGTGACGGAG TTTTTGAAGT TCTCTCAACC AACGGGGATA CTCACTTGGG AGGAGACGAC TTCGATGGAG TCATCATCAA CTGGATGCTT GATGAATTCA AAAAACAAGA AGGCATTGAT CTAAGCAAAG ATAACATGGC TTTGCAAAGA TTGAAAGATG CTGCTGAAAA AGCAAAAATA GAATTGTCTG GTGTATCGTC TACTGAAATC AATCAGCCAT TCATCACTAT CGACGCTAAT GGACCTAAAC ATTTGGCTTT AACTCTAACT CGCGCTCAAT TCGAACACCT AGCTTCCTCT CTCATTGAGC GAACCAAACA ACCTTGTGCT CAGGCTTTAA AAGATGCTAA ATTGTCCGCT TCTGACATTG ATGATGTTCT TCTAGTTGGC GGAATGTCCA GAATGCCTGC GGTACAAGCA GTTGTAAAAG AGATCTTTGG TAAAGAGCCT AATAAAGGCG TCAATCCAGA TGAAGTTGTA GCGATTGGAG CTGCTATTCA GGGTGGTGTC CTCGGCGGAG AAGTGAAAGA CGTTCTGTTG TTGGATGTGA TTCCCCTCTC TTTAGGAATT GAGACTCTAG GTGGGGTCAT GACTCCTTTG GTAGAGAGAA ACACTACAAT CCCTACTCAG AAGAAGCAAA TCTTCTCTAC AGCCGCTGAC AATCAGCCAG CAGTGACTAT CGTCGTTCTT CAAGGTGAAC GGCCTATGGC GAAAGACAAT AAGGAAATTG GAAGATTTGA TCTAACAGAC ATTCCTCCTG CTCCTCGCGG CCATCCACAA ATTGAGGTAA CCTTCGATAT TGATGCCAAC GGAATTTTAC ACGTTTCTGC TAAAGATGCT GCTAGTGGAC GCGAACAAAA AATCCGTATT GAAGCAAGCT CTGGATTAAA AGAAGATGAA ATTCAACAAA TGATCCGCGA TGCAGAGCTT CATAAAGAGG AAGACAAACA ACGAAAAGAA GCTTCTGATG TGAAAAATGA AGCCGATGGA ATGATCTTTA GAGCCGAAAA AGCTGTGAAA GATTACCACG ACAAAATTCC TGCAGAACTT GTTAAAGAAA TTGAAGAGCA TATTGAGAAA GTACGCCAAG CAATCAAAGA AGATGCTTCC ACAACAGCTA TCAAAGCAGC TTCTGATGAG TTGAGTACTC ATATGCAAAA AATCGGAGAA GCTATGCAGG CTCAATCCGC ATCCGCAGCA GCATCTTCTG CAGCGAATGC TCAAGGAGGG CCAAACATTA ACTCCGAAGA TCTGAAAAAA CATAGTTTCA GCACACGACC TCCAGCAGGA GGAAGCGCCT CTTCTACAGA CAACATTGAA GATGCTGATG TTGAAATTGT TGATAAACCT GAGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT