ChtrB.00169.a

Replicative DNA helicase (EC 3.6.4.12)

CENTER ID: ChtrB.00169.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.00169.a.B1.GE42402 full length 1 472
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_82005
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|272561.5.peg.541
UniProt: O84505
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATQTKKPQP TQLLSLPNSK ESEMIVLGCM LTSVNHLNLA ANLLQEDDFY FLEHRIIFRV LQDAFKSDRP MDPHLTGEEL KRRDQLNIIG GPSYLITLSE FAGTSAYIEE YAEIIRSKSI LRKMIQAAKD IEKKAAEEPR DVTTALDDAQ NLLFRISQTT NLAPHVLVAD KLKGVASSKD KSFLLALQER QEAFQASAHD SSSPMLSGFP THFLDLDKMI SGFSPSNLII LAARPAMGKT ALALNIVENF CFDSRLPVGI FSLEMTVDQL IHRIICSRSE VEAKKISVGD ISGRDFQRVV SVVREMEEHT LLIDDYPGLK ITDLRARARR MKESYDIQFL VIDYLQLISS SGNLRNSDSR NQEISEISRM LKNLARELNI PILCLSQLSR KVEDRANHRP LMSDLRESGS IEQDADQIMF LLRREYYDPN DKPGTAELIV AKNRHGSIGS VQLVFEKDFA RFRNYAGCEF PG
NT Sequence
atggcgaccc agacaaaaaa accccaaccc actcaactcc tctctcttcc taattctaag gaatctgaaa tgattgtact ggggtgtatg ttgaccagtg tcaatcattt gaaccttgct gccaaccttc ttcaggaaga tgatttctac ttcttagagc atcgtattat ttttcgtgtg ttgcaggacg ctttcaagtc tgaccgcccc atggatcccc atcttacggg agaagaactc aaacgccgag atcaactcaa tatcattgga ggcccctctt atctaatcac tctctctgaa tttgcaggga catctgccta tattgaagaa tacgcagaaa ttatccgctc caaatctatt ctaagaaaaa tgattcaagc ggccaaagat atcgaaaaga aagccgctga agagccacgt gacgtcacta ccgctttaga tgatgctcaa aacttattgt ttcgcataag ccaaacaact aacttagccc cccatgtcct tgttgcagac aaactcaaag gagttgcttc ttctaaagac aaatccttct tactcgcttt acaagaacgc caagaagctt tccaagcaag tgctcatgac tcaagctctc ctatgctctc aggcttcccc actcatttct tagatctaga taaaatgatt agtgggttca gcccctctaa cttgatcatt cttgctgctc gtcctgctat ggggaaaact gctttagctc ttaacattgt agagaatttt tgttttgata gccggcttcc tgtaggaatt ttttcattag agatgactgt agaccagtta atccatcgca tcatctgctc gcgttccgaa gtagaagcta aaaaaattag cgtgggagat atttctggta gagattttca acgcgtggtc tctgtggtaa gagagatgga agaacatact ctactcatag atgactaccc gggattgaaa atcacagatc ttcgagcacg cgcaagaaga atgaaagaaa gctacgatat ccaatttcta gttattgact acctacaatt aatctctagc tctgggaacc taagaaattc tgattcgcga aaccaagaaa tttctgaaat ctccagaatg cttaaaaatt tggctcgaga actcaatatt cctattctct gcctctctca attatctaga aaagtagaag acagagctaa tcacagacct ttgatgagcg atttaagaga aagtggaagc attgaacaag atgctgacca aattatgttt ttacttcgcc gcgaatatta tgatcctaat gataaacctg gaacagcaga gttgattgtg gctaaaaacc gccacggctc cattggatct gtacaattag tttttgaaaa agacttcgct cgattccgaa attatgctgg ctgtgagttc cctggataa
Details for ChtrB.00169.a.B1.GE42402
HARVESTED ON: 5/25/2018
SEQUENCED ON: 5/31/2018
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA TQTKKPQPTQ LLSLPNSKES EMIVLGCMLT SVNHLNLAAN LLQEDDFYFL EHRIIFRVLQ DAFKSDRPMD PHLTGEELKR RDQLNIIGGP SYLITLSEFA GTSAYIEEYA EIIRSKSILR KMIQAAKDIE KKAAEEPRDV TTALDDAQNL LFRISQTTNL APYVLVADKL KGVASSKDKS FLLALQERQE AFQASAHDSS SPMLSGFPTH FLDLDRMISG FSPSNLIILA ARPAMGKTAL ALNIVENFCF DSRLPVGIFS LEMTVDQLIH RIICSRSEVE AKKISVGDIS GRDFQRVVSV VREMEEHTLL IDDYPGLKIT DLRARARRMK ESYDIQFLVI DYLQLISSSG NLRNSDSRNQ EISEISRMLK NLARELNIPI LCLSQLSRKV EDRANHRPLM SDLRESGSIE QDADQIMFLL RREYYDPNDK PGTAELIVAK NRHGSIGSIQ LVFEKDFARF RN
Validated NT Sequence
taatttcgga atcgagcaaa gtctttttca aaaactaatt gtatagatcc aatggagccg tggcggtttt tagccacaat caactctgct gttccaggtt tatcattagg atcataatat tcgcggcgaa gtaaaaacat aatttggtca gcatcttgtt caatgcttcc actttctctt aaatcgctca tcaaaggtct gtgattagct ctgtcttcta cttttctaga taattgagag aggcagagaa taggaatatt gagttctcga gccaaatttt taagcattct ggagatttca gaaatttctt ggtttcgcga atcagaattt cttaggttcc cagagctaga gattaattgt aggtagtcaa taactagaaa ttggatatcg tagctttctt tcattcttct tgcgcgtgct cgaagatctg tgattttcaa ccccgggtag tcatctatga gtagagtatg ttcttccatc tctcttacca cagagaccac gcgttgaaaa tctctaccag aaatatctcc cacgctaatt tttttagctt ctacttcgga acgcgagcag atgatgcgat ggattaactg gtctacagtc atctctaatg aaaaaattcc tacaggaagc cggctatcaa aacaaaaatt ctctacaatg ttaagagcta aagcagtttt ccccatagca ggacgagcag caagaatgat caagttagag gggctgaacc cactaatcat tctatctaga tctaagaaat gagtggggaa gcctgagagc ataggagagc ttgagtcatg agcacttgct tggaaagctt cttggcgttc ttgtaaagcg agtaagaagg atttgtcttt agaagaagca actcctttga gtttgtctgc aacaaggaca tagggggcta agttagttgt ttggcttatg cgaaacaata agttttgagc atcatccaaa gcggtagtga cgtcacgtgg ctcttcagcg gctttctttt cgatatcttt ggccgcttga atcatttttc ttagaataga tttggagcgg ataatttctg cgtattcttc aatataggca gatgtccctg caaattcaga gagagtgatt agataagagg ggcctccaat gatattgagt tgatctcggc gtttgagttc ttctcccgta agatggggat ccatggggcg gtcagacttg aaagcgtcct gcaacacacg aaaaataata cgatgctcta agaagtagaa atcatcttcc tgaagaaggt tggcagcaag gttcaaatga ttgacactgg tcaacataca ccccagcaca atcatttcag attccttaga attaggaaga gagaggagtt gagtgggttg gggttttttt gtctgggtcg ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMA TQTKKPQPTQ LLSLPNSKES EMIVLGCMLT SVNHLNLAAN LLQEDDFYFL EHRIIFRVLQ DAFKSDRPMD PHLTGEELKR RDQLNIIGGP SYLITLSEFA GTSAYIEEYA EIIRSKSILR KMIQAAKDIE KKAAEEPRDV TTALDDAQNL LFRISQTTNL APHVLVADKL KGVASSKDKS FLLALQERQE AFQASAHDSS SPMLSGFPTH FLDLDKMISG FSPSNLIILA ARPAMGKTAL ALNIVENFCF DSRLPVGIFS LEMTVDQLIH RIICSRSEVE AKKISVGDIS GRDFQRVVSV VREMEEHTLL IDDYPGLKIT DLRARARRMK ESYDIQFLVI DYLQLISSSG NLRNSDSRNQ EISEISRMLK NLARELNIPI LCLSQLSRKV EDRANHRPLM SDLRESGSIE QDADQIMFLL RREYYDPNDK PGTAELIVAK NRHGSIGSVQ LVFEKDFARF RNYAGCEFPG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga cccagacaaa aaaaccccaa cccactcaac tcctctctct tcctaattct aaggaatctg aaatgattgt actggggtgt atgttgacca gtgtcaatca tttgaacctt gctgccaacc ttcttcagga agatgatttc tacttcttag agcatcgtat tatttttcgt gtgttgcagg acgctttcaa gtctgaccgc cccatggatc cccatcttac gggagaagaa ctcaaacgcc gagatcaact caatatcatt ggaggcccct cttatctaat cactctctct gaatttgcag ggacatctgc ctatattgaa gaatacgcag aaattatccg ctccaaatct attctaagaa aaatgattca agcggccaaa gatatcgaaa agaaagccgc tgaagagcca cgtgacgtca ctaccgcttt agatgatgct caaaacttat tgtttcgcat aagccaaaca actaacttag ccccccatgt ccttgttgca gacaaactca aaggagttgc ttcttctaaa gacaaatcct tcttactcgc tttacaagaa cgccaagaag ctttccaagc aagtgctcat gactcaagct ctcctatgct ctcaggcttc cccactcatt tcttagatct agataaaatg attagtgggt tcagcccctc taacttgatc attcttgctg ctcgtcctgc tatggggaaa actgctttag ctcttaacat tgtagagaat ttttgttttg atagccggct tcctgtagga attttttcat tagagatgac tgtagaccag ttaatccatc gcatcatctg ctcgcgttcc gaagtagaag ctaaaaaaat tagcgtggga gatatttctg gtagagattt tcaacgcgtg gtctctgtgg taagagagat ggaagaacat actctactca tagatgacta cccgggattg aaaatcacag atcttcgagc acgcgcaaga agaatgaaag aaagctacga tatccaattt ctagttattg actacctaca attaatctct agctctggga acctaagaaa ttctgattcg cgaaaccaag aaatttctga aatctccaga atgcttaaaa atttggctcg agaactcaat attcctattc tctgcctctc tcaattatct agaaaagtag aagacagagc taatcacaga cctttgatga gcgatttaag agaaagtgga agcattgaac aagatgctga ccaaattatg tttttacttc gccgcgaata ttatgatcct aatgataaac ctggaacagc agagttgatt gtggctaaaa accgccacgg ctccattgga tctgtacaat tagtttttga aaaagacttc gctcgattcc gaaattatgc tggctgtgag ttccctggat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat