ChtrB.00164.a

Arginine--tRNA ligase (EC 6.1.1.19) (Arginyl-tRNA synthetase) (ArgRS)

CENTER ID:	ChtrB.00164.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.00164.a.B1.GE42401	full length		1	563

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.00164.a.B1.PW38507	full length		1	563

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_82032

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|272561.5.peg.491
UniProt:	O84460

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTLLSFLTS LCSAAIHQAF PELEELTLDI TPSTKEHFGH YQCNDAMKLA RVLHKSPRAI AESIVAHIPP TPFSSIEIAG AGFINFTFSK EFLASQLQTF SKELANGFRA ASPQKVIIDF SSPNIAKDMH VGHLRSTIIG DCLARCFSFV GHDVLRLNHI GDWGTAFGML ITYLQETSQE AIHQLEDLTA LYKKAHARFA EDSEFKKRSQ HNVVALQSGD AQALALWKQI CSVSEKSFQT IYSILDVELH TRGESFYNPF LAEVVADLES KNLVTLSDGA KCVFHEAFSI PLMIQKSDGG YNYATTDVAA MRYRIQQDQA DRILIVTDSG QSLHFQLLEA TCLAAGYLPS KGIFSHVGFG LVLDTQGRKF KTRSGENIKL RELLDTAVEK AKESLKAHRP DISEEELAYQ GPILGINAIK YADLSSHRIN DYVFSFEKML RFEGNTAMSL LYAYVRIQGI KRRMGLESPP QEGPLAVHEP AEEALALTLL RFPEILDLTL RELCPHFLTD YLYALTNKFN AFFRDCHIEG SDSQQERLYL CGLTERTLST GMHLLGLKTL NHL

NT Sequence

atgacaacac ttctttcttt tctgacttcg ctatgttctg cagcgattca tcaagccttc cctgagttgg aagagctaac cttagacatc actccctcta ctaaggagca ttttggacat tatcaatgta acgatgcaat gaaacttgca cgtgtattac acaaatcccc tcgtgccatt gccgaatcga tcgttgcgca tattcctccc actccttttt cctctataga gattgcagga gctggattta tcaattttac tttctcaaaa gaattcctag ctagtcagct ccaaaccttc tcaaaagaat tagcaaatgg gtttcgtgct gcgtctcctc aaaaagttat tattgatttt tcttctccta atattgctaa agatatgcat gtaggccatc tgcgctccac gattatcgga gattgtttag cacgatgctt ttcctttgtc ggccatgacg tcttacgctt aaaccatatt ggagattggg gtacagcttt tggtatgcta atcacctatc tgcaagagac ctctcaggag gcgattcatc aactagaaga tctcactgca ttatataaaa aggctcacgc gcgtttcgca gaagactctg aatttaaaaa acgctcccaa cataacgttg tagccttaca atccggagat gctcaagctc ttgcactatg gaaacaaatc tgttccgttt ctgagaaatc ctttcagaca atctactcga ttttggatgt tgagctccat acacgcggcg aatcatttta taatcctttc ctagcagagg ttgtcgcaga cttagaatct aaaaaccttg tcacgctttc tgatggcgca aaatgcgtat tccatgaagc cttctctatt cctctcatga ttcaaaagag tgatggcgga tacaattacg caacaaccga tgtcgcagct atgcgctatc gcatccaaca agatcaggcc gatagaattc ttatcgttac agactcagga caatccttac acttccagct tctagaagca acgtgcttag cagcaggcta tcttccttct aaagggatct tttcacatgt aggatttgga cttgtcttgg atactcaagg aagaaaattc aaaacacgtt cgggagagaa catcaaatta cgagaacttc tcgatacagc agtggaaaaa gcgaaagagt ctctaaaagc acatcgtcca gacatctcag aagaagaact ggcatatcaa ggccctatcc ttggtattaa tgcaattaaa tatgcagacc tttcttctca tagaatcaat gactacgtgt tctctttcga gaagatgctc cgcttcgaag gaaatacagc gatgtctctc ctgtatgcct atgtacgtat ccaaggaatt aaacgcagaa tgggattaga atctccgcct caagaagggc ctcttgctgt tcatgagcct gcagaagaag cgttagcact tactctttta cgtttccctg aaattttgga cctcaccctc agagaactct gtcctcattt cctaactgac tatctctatg cactcaccaa taagttcaat gctttcttcc gcgattgcca tatcgaagga tccgattctc agcaagaacg tctttatctc tgcggactta ccgaacgaac gctatcaaca ggtatgcact tactaggtct taaaaccttg aatcacctgt aa

Details for ChtrB.00164.a.B1.GE42401

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	64kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	95
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMT TLLSFLTSLC SAAIHQAFPE LEELTLDITP STKEHFGHYQ CNDAMKLARV LRKSPRAIAE SIVAHIPPAP FSSIEIAGAG FINFTFSKEF LASQLQTFSK ELANGFRAAS PQKVIIDFSS PNIAKDMHVG HLRSTIIGDC LARCFSFVGH DVLRLNHIGD WGTAFGMLIT YLQETSQEAI HQLEDLTALY KKAHARFAED SEFKKRSQHN VVALQSGDAQ ALALWKQICS VSEKSFQTIY SILDVELHTR GESFYNPFLA EVVADLESKN LVTLSDGAKX XXXXXXXXXX XXXXXXXXXX XATTDVAAMR YRIXQDQADR ILIVTDSGQS LHFQLLEATC LAAGYLPSKG IFSHVGFGLV LDTQGRKFKT RSGENIKLRE LLDTAVEKAK ESLKAHRPDI SEEELAYQGP ILGINAIKYA DLSSHRINDY VFSFEKMLRF EGNTAMSLLY AYVRIQGIKR RMGLESLPQE GPLAIHEPAE EALALTLLRF PEILDLTLRE LCPHFLTDYL YALTNKFNTF FRDCHIEGSD SQQERLYLCG LTERTLSTGM HLL

Validated NT Sequence

acctagtaag tgcatacctg ttgatagcgt tcgttcggta agtccgcaga gataaagacg ttcttgctga gaatcggatc cttcgatatg gcaatcgcgg aagaaagtat tgaacttatt ggtgagtgca tagagatagt cagttaggaa atgaggacag agttctctga gggtgaggtc caaaatttca gggaaacgta aaagagtaag tgctaacgct tcttctgcag gctcatgaat agcaagaggc ccttcttgag gcagagattc taatcccatt ctgcgtttaa ttccttggat acgtacatag gcatacagga gagacatcgc tgtatttcct tcgaagcgga gcatcttctc gaaagagaac acgtagtcat tgattctatg agaagaaagg tctgcatatt taattgcatt aataccaagg atagggcctt gatatgccag ttcttcttct gagatgtctg gacgatgtgc ttttagagac tctttcgctt tttccactgc tgtatcgaga agttctcgta atttgatgtt ctctcccgaa cgtgttttga attttcttcc ttgagtatcc aagacaagtc cgaatcctac atgtgaaaag atccctttag aaggaagata gcctgctgct aagcacgttg cttctagaag ctggaagtgt aaggattgtc ctgagtctgt aacgataaga attctatcgg cctgatcttg nnggatgcga tagcgcatag ctgcgacatc ggttgttgcn nnnnnnnnnn nnnnnnnnnn ntttnnnnnn nngnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncattttg cgccatcaga aagcgtgaca aggtttttag attctaagtc tgcgacaacc tctgctagga aaggattata aaatgattcg ccgcgtgtat ggagctcaac atccaaaatc gagtagattg tctgaaagga tttctcagaa acggaacaga tttgtttcca tagtgcaaga gcttgagcat ctccggattg taaggctaca acgttatgtt gggagcgttt tttaaattca gagtcttctg cgaaacgcgc gtgagctttt ttatataatg cagtgagatc ttctagttga tgaatcgcct cctgagaggt ctcttgcaga taggtgatta gcataccaaa agctgtaccc caatctccaa tatggtttaa gcgtaagacg tcatggccga caaaggaaaa gcatcgtgct aaacaatctc cgataatcgt ggagcgcaga tggcctacat gcatatcttt agcaatatta ggagaagaaa aatcaataat aactttttga ggagacgcag cacgaaaccc atttgctaat tcttttgaga aggtttggag ctgactagct aggaattctt ttgagaaagt aaaattgata aatccagctc ctgcaatctc tatagaggaa aaaggagcgg gaggaatatg cgcaacgatc gattcggcaa tggcacgagg ggatttgcgt aatacacgtg caagtttcat tgcatcgtta cattgataat gtccaaaatg ctccttagta gagggagtga tgtctaaggt tagctcttcc aactcaggga aggcttgatg aatcgctgca gaacatagcg aagtcagaaa agaaagaagt gttgtcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHMT TLLSFLTSLC SAAIHQAFPE LEELTLDITP STKEHFGHYQ CNDAMKLARV LHKSPRAIAE SIVAHIPPTP FSSIEIAGAG FINFTFSKEF LASQLQTFSK ELANGFRAAS PQKVIIDFSS PNIAKDMHVG HLRSTIIGDC LARCFSFVGH DVLRLNHIGD WGTAFGMLIT YLQETSQEAI HQLEDLTALY KKAHARFAED SEFKKRSQHN VVALQSGDAQ ALALWKQICS VSEKSFQTIY SILDVELHTR GESFYNPFLA EVVADLESKN LVTLSDGAKC VFHEAFSIPL MIQKSDGGYN YATTDVAAMR YRIQQDQADR ILIVTDSGQS LHFQLLEATC LAAGYLPSKG IFSHVGFGLV LDTQGRKFKT RSGENIKLRE LLDTAVEKAK ESLKAHRPDI SEEELAYQGP ILGINAIKYA DLSSHRINDY VFSFEKMLRF EGNTAMSLLY AYVRIQGIKR RMGLESPPQE GPLAVHEPAE EALALTLLRF PEILDLTLRE LCPHFLTDYL YALTNKFNAF FRDCHIEGSD SQQERLYLCG LTERTLSTGM HLLGLKTLNH L

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa cacttctttc ttttctgact tcgctatgtt ctgcagcgat tcatcaagcc ttccctgagt tggaagagct aaccttagac atcactccct ctactaagga gcattttgga cattatcaat gtaacgatgc aatgaaactt gcacgtgtat tacacaaatc ccctcgtgcc attgccgaat cgatcgttgc gcatattcct cccactcctt tttcctctat agagattgca ggagctggat ttatcaattt tactttctca aaagaattcc tagctagtca gctccaaacc ttctcaaaag aattagcaaa tgggtttcgt gctgcgtctc ctcaaaaagt tattattgat ttttcttctc ctaatattgc taaagatatg catgtaggcc atctgcgctc cacgattatc ggagattgtt tagcacgatg cttttccttt gtcggccatg acgtcttacg cttaaaccat attggagatt ggggtacagc ttttggtatg ctaatcacct atctgcaaga gacctctcag gaggcgattc atcaactaga agatctcact gcattatata aaaaggctca cgcgcgtttc gcagaagact ctgaatttaa aaaacgctcc caacataacg ttgtagcctt acaatccgga gatgctcaag ctcttgcact atggaaacaa atctgttccg tttctgagaa atcctttcag acaatctact cgattttgga tgttgagctc catacacgcg gcgaatcatt ttataatcct ttcctagcag aggttgtcgc agacttagaa tctaaaaacc ttgtcacgct ttctgatggc gcaaaatgcg tattccatga agccttctct attcctctca tgattcaaaa gagtgatggc ggatacaatt acgcaacaac cgatgtcgca gctatgcgct atcgcatcca acaagatcag gccgatagaa ttcttatcgt tacagactca ggacaatcct tacacttcca gcttctagaa gcaacgtgct tagcagcagg ctatcttcct tctaaaggga tcttttcaca tgtaggattt ggacttgtct tggatactca aggaagaaaa ttcaaaacac gttcgggaga gaacatcaaa ttacgagaac ttctcgatac agcagtggaa aaagcgaaag agtctctaaa agcacatcgt ccagacatct cagaagaaga actggcatat caaggcccta tccttggtat taatgcaatt aaatatgcag acctttcttc tcatagaatc aatgactacg tgttctcttt cgagaagatg ctccgcttcg aaggaaatac agcgatgtct ctcctgtatg cctatgtacg tatccaagga attaaacgca gaatgggatt agaatctccg cctcaagaag ggcctcttgc tgttcatgag cctgcagaag aagcgttagc acttactctt ttacgtttcc ctgaaatttt ggacctcacc ctcagagaac tctgtcctca tttcctaact gactatctct atgcactcac caataagttc aatgctttct tccgcgattg ccatatcgaa ggatccgatt ctcagcaaga acgtctttat ctctgcggac ttaccgaacg aacgctatca acaggtatgc acttactagg tcttaaaacc ttgaatcacc tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for ChtrB.00164.a.B1.PW38507

PURIFICATION DATe:	7/9/2018
CONCENTRATION:	13.19mg/ml
OBSERVED MW:	60kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	95
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAA CACTTCTTTC TTTTCTGACT TCGCTATGTT CTGCAGCGAT TCATCAAGCC TTCCCTGAGT TGGAAGAGCT AACCTTAGAC ATCACTCCCT CTACTAAGGA GCATTTTGGA CATTATCAAT GTAACGATGC AATGAAACTT GCACGTGTAT TACACAAATC CCCTCGTGCC ATTGCCGAAT CGATCGTTGC GCATATTCCT CCCACTCCTT TTTCCTCTAT AGAGATTGCA GGAGCTGGAT TTATCAATTT TACTTTCTCA AAAGAATTCC TAGCTAGTCA GCTCCAAACC TTCTCAAAAG AATTAGCAAA TGGGTTTCGT GCTGCGTCTC CTCAAAAAGT TATTATTGAT TTTTCTTCTC CTAATATTGC TAAAGATATG CATGTAGGCC ATCTGCGCTC CACGATTATC GGAGATTGTT TAGCACGATG CTTTTCCTTT GTCGGCCATG ACGTCTTACG CTTAAACCAT ATTGGAGATT GGGGTACAGC TTTTGGTATG CTAATCACCT ATCTGCAAGA GACCTCTCAG GAGGCGATTC ATCAACTAGA AGATCTCACT GCATTATATA AAAAGGCTCA CGCGCGTTTC GCAGAAGACT CTGAATTTAA AAAACGCTCC CAACATAACG TTGTAGCCTT ACAATCCGGA GATGCTCAAG CTCTTGCACT ATGGAAACAA ATCTGTTCCG TTTCTGAGAA ATCCTTTCAG ACAATCTACT CGATTTTGGA TGTTGAGCTC CATACACGCG GCGAATCATT TTATAATCCT TTCCTAGCAG AGGTTGTCGC AGACTTAGAA TCTAAAAACC TTGTCACGCT TTCTGATGGC GCAAAATGCG TATTCCATGA AGCCTTCTCT ATTCCTCTCA TGATTCAAAA GAGTGATGGC GGATACAATT ACGCAACAAC CGATGTCGCA GCTATGCGCT ATCGCATCCA ACAAGATCAG GCCGATAGAA TTCTTATCGT TACAGACTCA GGACAATCCT TACACTTCCA GCTTCTAGAA GCAACGTGCT TAGCAGCAGG CTATCTTCCT TCTAAAGGGA TCTTTTCACA TGTAGGATTT GGACTTGTCT TGGATACTCA AGGAAGAAAA TTCAAAACAC GTTCGGGAGA GAACATCAAA TTACGAGAAC TTCTCGATAC AGCAGTGGAA AAAGCGAAAG AGTCTCTAAA AGCACATCGT CCAGACATCT CAGAAGAAGA ACTGGCATAT CAAGGCCCTA TCCTTGGTAT TAATGCAATT AAATATGCAG ACCTTTCTTC TCATAGAATC AATGACTACG TGTTCTCTTT CGAGAAGATG CTCCGCTTCG AAGGAAATAC AGCGATGTCT CTCCTGTATG CCTATGTACG TATCCAAGGA ATTAAACGCA GAATGGGATT AGAATCTCCG CCTCAAGAAG GGCCTCTTGC TGTTCATGAG CCTGCAGAAG AAGCGTTAGC ACTTACTCTT TTACGTTTCC CTGAAATTTT GGACCTCACC CTCAGAGAAC TCTGTCCTCA TTTCCTAACT GACTATCTCT ATGCACTCAC CAATAAGTTC AATGCTTTCT TCCGCGATTG CCATATCGAA GGATCCGATT CTCAGCAAGA ACGTCTTTAT CTCTGCGGAC TTACCGAACG AACGCTATCA ACAGGTATGC ACTTACTAGG TCTTAAAACC TTGAATCACC TGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT