ChtrB.00163.a

Phenylalanine--tRNA ligase alpha subunit (EC 6.1.1.20) (Phenylalanyl-tRNA synthetase alpha subunit) (PheRS)

CENTER ID: ChtrB.00163.a
ORGANISM: Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChtrB.00163.a.B4.GE42575 truncation 3 89 342
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD RESULTS
comparative modelling Robetta_82008
de-novo with evolutionary constraints ModelArchive_ma-4jcjk

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272561.5.peg.923
UniProt: O84843

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIQEELEAV KQQFSCDVSL AHSSKDLFDV KVKYLGKKGI FRGFADQLRK YPIEQKATVG ASINACKQYV EEVLLERGKA VLAKEEAEEF LKEKIDISLP GSEEAALGGK HVIKKVLDDV VDIFVRFGFC VREAPNIESE KNNFSLLNFE EDHPARQMQD TFYLDPTTVL RTHTSNVQSR ELARNKPPVR IVAPGECFRN EDVSARSHVI FHQVEAFCVD KDISFSDLTS MLAGFYHIFF GRKVELRFRH SYFPFVEPGI EVDISCECHG AGCSLCKHAG WLEVAGAGMI HPNVLRKASI DPEEYSGYAL GMGIERLAML KYGISDIRLF SENDLRFLRQ FS
NT Sequence
atgacaattc aagaggaact tgaggctgtt aaacagcagt ttagttgtga tgtaagcctt gcgcattctt ctaaagatct tttcgatgtg aaagtaaaat acctgggaaa gaagggaatc tttcgaggtt ttgctgatca gttgaggaag taccctatag agcagaaagc gactgttggc gcttccatta acgcttgtaa gcaatacgtg gaggaagttt tactcgagag aggcaaggcc gttttggcta aagaagaagc agaagagttc cttaaggaga agatagatat cagtttacct ggtagcgaag aagctgctct tggtggtaag catgttatca agaaagtcct tgatgatgtt gtagatatct ttgttcgctt tggattttgt gttcgggaag ctcctaatat cgaaagtgaa aaaaacaatt tttctcttct taatttcgaa gaagatcatc ctgctcgaca gatgcaggat actttctatt tggatcccac cacggtcttg cgtacgcaca cgtcgaatgt gcagtctcgg gagttagcga gaaacaaacc tcctgttaga attgtcgctc caggagagtg tttccgtaat gaagacgttt ctgcgcgttc gcatgtgatt tttcaccaag tagaggcttt ctgcgtagat aaagatattt ctttttcaga cttgacatcg atgttggcag ggttttacca tatcttcttt ggacgcaaag tggagttgcg gtttagacac agctatttcc cttttgtcga gccagggatc gaggtagaca tttcttgtga atgtcatgga gccggatgtt ctttgtgtaa gcatgctggt tggttggaag ttgctggagc aggaatgatt catccgaatg tcttgcgtaa ggcaagcatt gatccagaag agtattctgg gtatgccttg gggatgggta tagagcgtct cgcgatgctc aagtacggta tttccgatat tcgattgttt agtgagaacg atttgcggtt tttacggcaa ttttcttaa
Details for ChtrB.00163.a.B4.GE42575
HARVESTED ON: 8/5/2018
SEQUENCED ON: 8/8/2018
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME FLKEKIDISL PGSEEAALGG KHVIKKVLDD VVDIFVRFGF CVREAPNIES EKNNFSLLNF EEDHPARQMQ DTFYLDPTTV LRTHTSNVQS RELARNKPPV RIVAPGECFR NEDVSARSHV IFHQVEAFCV DKDISFSDLT SMLAGFYHIF FGRKVELRFR HSYFPFVEPG IEVDISCECH GAGCSLCKHS GWLEVAGAGM IHPNVLRKAS FDPEEYSGYA LGMGIERLAM LKYGISDIRL FSENDLRFLR QFS
Validated NT Sequence
atcttactca cttaagaaaa ttgccgtaaa aaccgcaaat cgttctcact aaacaatcga atatcggaaa taccatactt gagcatcgcg agacgctcta tacccatccc caaggcatac ccagaatact cttctggatc aaagcttgcc ttacgcaaga cattcggatg aatcattcct gctccagcaa cttccaacca accagaatgc ttacacaaag aacatccggc tccatgacat tcacaagaaa tgtctacctc gatccctggc tcgacaaaag ggaaatagct gtgtctaaac cgcaactcca ctttgcgtcc aaagaagata tggtaaaacc ctgccaacat cgatgtcaag tctgaaaaag aaatatcttt atctacgcag aaagcctcta cttggtgaaa aatcacatgc gaacgcgcag aaacgtcttc attacggaaa cactctcctg gagcgacaat tctaacagga ggtttgtttc tcgctaactc ccgagactgc acattcgacg tgtgcgtacg caagaccgtg gtgggatcca aatagaaagt atcctgcatc tgtcgagcag gatgatcttc ttcgaaatta agaagagaaa aattgttttt ttcactttcg atattaggag cttcccgaac acaaaatcca aagcgaacaa agatatctac aacatcatca aggactttct tgataacatg cttaccacca agagcagctt cttcgctacc aggtaaactg atatctatct tctccttaag gaactccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHEF LKEKIDISLP GSEEAALGGK HVIKKVLDDV VDIFVRFGFC VREAPNIESE KNNFSLLNFE EDHPARQMQD TFYLDPTTVL RTHTSNVQSR ELARNKPPVR IVAPGECFRN EDVSARSHVI FHQVEAFCVD KDISFSDLTS MLAGFYHIFF GRKVELRFRH SYFPFVEPGI EVDISCECHG AGCSLCKHAG WLEVAGAGMI HPNVLRKASI DPEEYSGYAL GMGIERLAML KYGISDIRLF SENDLRFLRQ FS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgagttcc ttaaggagaa gatagatatc agtttacctg gtagcgaaga agctgctctt ggtggtaagc atgttatcaa gaaagtcctt gatgatgttg tagatatctt tgttcgcttt ggattttgtg ttcgggaagc tcctaatatc gaaagtgaaa aaaacaattt ttctcttctt aatttcgaag aagatcatcc tgctcgacag atgcaggata ctttctattt ggatcccacc acggtcttgc gtacgcacac gtcgaatgtg cagtctcggg agttagcgag aaacaaacct cctgttagaa ttgtcgctcc aggagagtgt ttccgtaatg aagacgtttc tgcgcgttcg catgtgattt ttcaccaagt agaggctttc tgcgtagata aagatatttc tttttcagac ttgacatcga tgttggcagg gttttaccat atcttctttg gacgcaaagt ggagttgcgg tttagacaca gctatttccc ttttgtcgag ccagggatcg aggtagacat ttcttgtgaa tgtcatggag ccggatgttc tttgtgtaag catgctggtt ggttggaagt tgctggagca ggaatgattc atccgaatgt cttgcgtaag gcaagcattg atccagaaga gtattctggg tatgccttgg ggatgggtat agagcgtctc gcgatgctca agtacggtat ttccgatatt cgattgttta gtgagaacga tttgcggttt ttacggcaat tttcttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat