ChtrB.00156.a

Threonine--tRNA ligase (EC 6.1.1.3) (Threonyl-tRNA synthetase) (ThrRS)

CENTER ID:	ChtrB.00156.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.00156.a.B1.GE42400	full length		1	635
ChtrB.00156.a.B2.GE44186	TEdwards Stma structure domain		233	635

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.00156.a.B1.PW38521	full length		1	635

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_82030

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|272561.5.peg.633
UniProt:	O84585

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIHVTCNQEA FELPEGASAM DLANKMKQSH CFAGALINDQ EKDLSTTLQD GDTVLFLTWD DPKGREIFLH TSAHILAQAV LRLWPSAQPT IGPVIDQGFY YDFANLSISE EDFPAIEAMA KTIAEEKFPI SRQVFPDKEA ALAYFSQNPF KAELIAELPE EVEISAYTQG EFLDLCRGPH LPSTAPVKAF KLLRTSSAYW KGDPSRESLI RIYGVSFPTT KELKEHLHQL EEAKKRDHRV LGTKLDLFSQ QTCSAGMPFF HPRGMVVWNA LVDYWKRLHQ RAGYQQIQTP QLMNRELWEI SGHWENYKEN MYTLTVDEED YAIKPMNCPG CMLYYKTQLH SYREFPLRIA EIGHVHRHEL SGALSGLMRV RTFHQDDAHV FLTPEQVEEE TLNILNLVSE LYGTFGLEYH LELSTRPEQG TIGSDDLWEL ATKALKRALV KSQKPFIISP GEGAFYGPKI DIHVKDAINR TWQCGTIQLD MFLPERFDLK YTNAQGEKST PIMLHRALFG SIERFLGILI EHFKGRFPLW LSPEHVRIIT VADRHEARAQ ELAKHFSQMG IIVSVDSSNE SVSKKIRNAQ NMQVNYMITI GDKELETHLL AVRTRDNRVL NDIAVEQFSH VILEELRSLS LTPSL

NT Sequence

atgatccacg taacttgcaa tcaagaagct ttcgaactac cagaaggcgc ctcggcgatg gatcttgcaa ataagatgaa acaatctcat tgctttgcag gggcccttat taatgatcaa gaaaaggatc tttccaccac cttacaagac ggtgataccg ttctattcct cacttgggat gatcctaaag gtagagaaat ctttttacac acttcagcac atattctagc tcaagccgtt ctacgcttat ggccttcggc acagccaacg attggtccag tcatcgatca agggttctac tatgattttg ccaatctttc catcagcgaa gaagatttcc cggctataga agctatggcc aaaacgatcg cagaagaaaa attccctatc tcgcgtcagg ttttcccaga caaagaagct gctcttgcct atttttctca aaatcccttt aaagcagagc tcattgctga gcttcctgaa gaagtagaaa tttccgcgta tacgcaagga gaattcttgg atttatgtcg aggcccgcac cttccttcga cagctcctgt taaagctttc aagctattac gcacatcttc tgcttattgg aaaggtgacc cttctagaga atcgctgatt cgaatatatg gagtctcttt ccctacgacc aaggaactga aagaacatct tcatcagcta gaagaagcaa aaaaacgcga ccatcgagtt cttggaacaa agttagatct gttctctcaa caaacttgtt ctgctggaat gccttttttc catcctcggg gtatggttgt ttggaacgct ttagttgact actggaaacg ccttcatcag cgtgctggct atcagcaaat ccaaactccc caactcatga atagagagtt atgggagatc tctggccatt gggaaaatta caaagaaaac atgtataccc tcaccgtaga tgaagaagat tatgcaatta aacccatgaa ttgtcctggc tgcatgctgt actataaaac ccagctacac agctaccgag aattcccctt acggattgca gaaatcggcc atgtacatag acacgagctt tctggagctc tatcaggctt gatgcgagtg cgtactttcc atcaagatga cgcacacgta tttttaacac ctgagcaagt tgaagaggaa acactcaata tcctaaacct tgtttccgaa ctctatggaa cttttggact cgagtatcac ttagaacttt ccactcgtcc tgaacaagga accattggaa gcgatgatct ttgggaactt gcaacaaagg ctttgaaaag agctctcgtt aaatcgcaaa aaccttttat cataagccca ggagaaggag ccttttacgg tccaaaaatc gatatccatg taaaagatgc gatcaacagg acttggcaat gtggaacgat tcagttagat atgttcctgc cggaacgatt cgatctgaag tacacaaatg cacaaggaga gaaaagtaca cctattatgc tccatagagc tttgtttgga tctattgagc gattcttagg cattttaatc gaacatttca aaggacggtt ccctttgtgg ctaagtccag agcatgttcg cattattacc gtcgcagatc gccacgaggc tcgtgcccaa gaattggcaa aacatttcag ccaaatggga atcattgtct ctgttgattc atctaatgag tctgttagta agaaaattcg caatgcgcaa aacatgcaag tgaattacat gatcaccatt ggagataagg aattagagac ccacttacta gcagtgcgca cacgagacaa tcgtgtgttg aacgatattg ctgtcgaaca gttctctcat gttattcttg aagagcttcg ctctttatcc ttgactcctt ccttatag

Details for ChtrB.00156.a.B1.GE42400

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	74kDa
OBSERVED MW:	74kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMI HVTCNQEAFE LPEGASAMDL ANKMKQSHCF AGALINDQEK DLSTTLQDGD TVLFLTWDDP KGREIFLHTS AHILAQAVLR LWPSAQPTIG PVIDQGFYYD FANLSISEED FPAIEAMAKT IAEEKFPISR QVFPDKEAAL AYFSQNPFKA ELIAELPEEV EISAYTQGEF LDLCRGPHLP STAPVKAFKL LRTSSAYWKG DPSRESLIRI YGVSFPTTKE LKEHLHQLEE AKKRDHRVLG TKLDLFSQQT CSAGMPFFHP RGMVVWNALV DYWKRLHQRA GYQQIQTPQL MNRELWEISG HWENYKENMY TLTVDEEDYA IKPMNCPGCM LYYKTQLHSY REFPLRIAEI GHVHRHELSG ALSGLMRVRT FHQDDAHVFL TPEQVEEETL NILNLVSELY GTFGLEYHLE LSTRPEQGTI GSDDLWELAT KALKRALVKS QKPFIISPGE GAFYGPKIDI HVKDAINRTW QCGTIQLDMF LPERFDLKYT NAQGEKSTPI MLHRALFGSI ERFLGILIEH FKGRFPLWLS PEHVRIITVA DRHEARAQEL AKHFSQMGII VSVDSSNESV SKKIRNAQNM QVNYMITIGD KELETHLLAV RTRDNRVLND IAVEQFSHVI LEELRSLSLT PSL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcc acgtaacttg caatcaagaa gctttcgaac taccagaagg cgcctcggcg atggatcttg caaataagat gaaacaatct cattgctttg caggggccct tattaatgat caagaaaagg atctttccac caccttacaa gacggtgata ccgttctatt cctcacttgg gatgatccta aaggtagaga aatcttttta cacacttcag cacatattct agctcaagcc gttctacgct tatggccttc ggcacagcca acgattggtc cagtcatcga tcaagggttc tactatgatt ttgccaatct ttccatcagc gaagaagatt tcccggctat agaagctatg gccaaaacga tcgcagaaga aaaattccct atctcgcgtc aggttttccc agacaaagaa gctgctcttg cctatttttc tcaaaatccc tttaaagcag agctcattgc tgagcttcct gaagaagtag aaatttccgc gtatacgcaa ggagaattct tggatttatg tcgaggcccg caccttcctt cgacagctcc tgttaaagct ttcaagctat tacgcacatc ttctgcttat tggaaaggtg acccttctag agaatcgctg attcgaatat atggagtctc tttccctacg accaaggaac tgaaagaaca tcttcatcag ctagaagaag caaaaaaacg cgaccatcga gttcttggaa caaagttaga tctgttctct caacaaactt gttctgctgg aatgcctttt ttccatcctc ggggtatggt tgtttggaac gctttagttg actactggaa acgccttcat cagcgtgctg gctatcagca aatccaaact ccccaactca tgaatagaga gttatgggag atctctggcc attgggaaaa ttacaaagaa aacatgtata ccctcaccgt agatgaagaa gattatgcaa ttaaacccat gaattgtcct ggctgcatgc tgtactataa aacccagcta cacagctacc gagaattccc cttacggatt gcagaaatcg gccatgtaca tagacacgag ctttctggag ctctatcagg cttgatgcga gtgcgtactt tccatcaaga tgacgcacac gtatttttaa cacctgagca agttgaagag gaaacactca atatcctaaa ccttgtttcc gaactctatg gaacttttgg actcgagtat cacttagaac tttccactcg tcctgaacaa ggaaccattg gaagcgatga tctttgggaa cttgcaacaa aggctttgaa aagagctctc gttaaatcgc aaaaaccttt tatcataagc ccaggagaag gagcctttta cggtccaaaa atcgatatcc atgtaaaaga tgcgatcaac aggacttggc aatgtggaac gattcagtta gatatgttcc tgccggaacg attcgatctg aagtacacaa atgcacaagg agagaaaagt acacctatta tgctccatag agctttgttt ggatctattg agcgattctt aggcatttta atcgaacatt tcaaaggacg gttccctttg tggctaagtc cagagcatgt tcgcattatt accgtcgcag atcgccacga ggctcgtgcc caagaattgg caaaacattt cagccaaatg ggaatcattg tctctgttga ttcatctaat gagtctgtta gtaagaaaat tcgcaatgcg caaaacatgc aagtgaatta catgatcacc attggagata aggaattaga gacccactta ctagcagtgc gcacacgaga caatcgtgtg ttgaacgata ttgctgtcga acagttctct catgttattc ttgaagagct tcgctcttta tccttgactc cttccttatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for ChtrB.00156.a.B2.GE44186

HARVESTED ON:	4/1/2021
SEQUENCED ON:	4/13/2021
EXPECTED MW:	48kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMA KKRDHRVLGT KLDLFSQQTC SAGMPFFHPR GMVVWNALVD YWKRLHQRAG YQQIQTPQLM NRELWEISGH WENYKENMYT LTVDEEDYAI KPMNCPGCML YYKTQLHSYR EFPLRIAEIG HVHRHELSGA LSGLMRVRTF HQDDAHVFLT PEQVEEETLN ILNLVSELYG TFGLEYHLEL STRPEQGTIG SDDLWELATK ALKRALVKSQ XPFIISPGEG AFYGPKIDIH VKDAINRTWQ CGTIQLDMFL PERFDLKYTN AQGEKSTPIM LHRALFGSIE RFLGILIEHF KGRFPLWLSP EHVRIITVAD RHEARAQELA KHFSQMGIIV SVDSSNESVS KKIRNAQNMQ VNYMITIGDK ELETHLLAVR TRDNRVLNDI AVEQFSHVIL EEL

Validated NT Sequence

gaagctcttc aagaataaca tgagagaact gttcgacagc aatatcgttc aacacacgat tgtctcgtgt gcgcactgct agtaagtggg tctctaattc cttatctcca atggtgatca tgtaattcac ttgcatgttt tgcgcattgc gaattttctt actaacagac tcattagatg aatcaacaga gacaatgatt cccatttggc tgaaatgttt tgccaattct tgggcacgag cctcgtggcg atctgcgacg gtaataatgc gaacatgctc tggacttagc cacaaaggga accgtccttt gaaatgttcg attaaaatgc ctaagaatcg ctcaatagat ccaaacaaag ctctatggag cataataggt gtacttttct ctccttgtgc atttgtgtac ttcagatcga atcgttccgg caggaacata tctaactgaa tcgttccaca ttgccaagtc ctgttgatcg catcttttac atggatatcg atttttggac cgtaaaaggc tccttctcct gggcttatga taaaaggntt ttgcgattta acgagagctc ttttcaaagc ctttgttgca agttcccaaa gatcatcgct tccaatggtt ccttgttcag gacgagtgga aagttctaag tgatactcga gtccaaaagt tccatagagt tcggaaacaa ggtttaggat attgagtgtt tcctcttcaa cttgctcagg tgttaaaaat acgtgtgcgt catcttgatg gaaagtacgc actcgcatca agcctgatag agctccagaa agctcgtgtc tatgtacatg gccgatttct gcaatccgta aggggaattc tcggtagctg tgtagctggg ttttatagta cagcatgcag ccaggacaat tcatgggttt aattgcataa tcttcttcat ctacggtgag ggtatacatg ttttctttgt aattttccca atggccagag atctcccata actctctatt catgagttgg ggagtttgga tttgctgata gccagcacgc tgatgaaggc gtttccagta gtcaactaaa gcgttccaaa caaccatacc ccgaggatgg aaaaaaggca ttccagcaga acaagtttgt tgagagaaca gatctaactt tgttccaaga actcgatggt cgcgtttttt tgccatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHAK KRDHRVLGTK LDLFSQQTCS AGMPFFHPRG MVVWNALVDY WKRLHQRAGY QQIQTPQLMN RELWEISGHW ENYKENMYTL TVDEEDYAIK PMNCPGCMLY YKTQLHSYRE FPLRIAEIGH VHRHELSGAL SGLMRVRTFH QDDAHVFLTP EQVEEETLNI LNLVSELYGT FGLEYHLELS TRPEQGTIGS DDLWELATKA LKRALVKSQK PFIISPGEGA FYGPKIDIHV KDAINRTWQC GTIQLDMFLP ERFDLKYTNA QGEKSTPIML HRALFGSIER FLGILIEHFK GRFPLWLSPE HVRIITVADR HEARAQELAK HFSQMGIIVS VDSSNESVSK KIRNAQNMQV NYMITIGDKE LETHLLAVRT RDNRVLNDIA VEQFSHVILE ELRSLSLTPS L

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcaaaaa aacgcgacca tcgagttctt ggaacaaagt tagatctgtt ctctcaacaa acttgttctg ctggaatgcc ttttttccat cctcggggta tggttgtttg gaacgcttta gttgactact ggaaacgcct tcatcagcgt gctggctatc agcaaatcca aactccccaa ctcatgaata gagagttatg ggagatctct ggccattggg aaaattacaa agaaaacatg tataccctca ccgtagatga agaagattat gcaattaaac ccatgaattg tcctggctgc atgctgtact ataaaaccca gctacacagc taccgagaat tccccttacg gattgcagaa atcggccatg tacatagaca cgagctttct ggagctctat caggcttgat gcgagtgcgt actttccatc aagatgacgc acacgtattt ttaacacctg agcaagttga agaggaaaca ctcaatatcc taaaccttgt ttccgaactc tatggaactt ttggactcga gtatcactta gaactttcca ctcgtcctga acaaggaacc attggaagcg atgatctttg ggaacttgca acaaaggctt tgaaaagagc tctcgttaaa tcgcaaaaac cttttatcat aagcccagga gaaggagcct tttacggtcc aaaaatcgat atccatgtaa aagatgcgat caacaggact tggcaatgtg gaacgattca gttagatatg ttcctgccgg aacgattcga tctgaagtac acaaatgcac aaggagagaa aagtacacct attatgctcc atagagcttt gtttggatct attgagcgat tcttaggcat tttaatcgaa catttcaaag gacggttccc tttgtggcta agtccagagc atgttcgcat tattaccgtc gcagatcgcc acgaggctcg tgcccaagaa ttggcaaaac atttcagcca aatgggaatc attgtctctg ttgattcatc taatgagtct gttagtaaga aaattcgcaa tgcgcaaaac atgcaagtga attacatgat caccattgga gataaggaat tagagaccca cttactagca gtgcgcacac gagacaatcg tgtgttgaac gatattgctg tcgaacagtt ctctcatgtt attcttgaag agcttcgctc tttatccttg actccttcct tatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for ChtrB.00156.a.B1.PW38521

PURIFICATION DATe:	9/17/2018
CONCENTRATION:	8.73mg/ml
OBSERVED MW:	75kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	4
VIAL VOLUME:	100µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCC ACGTAACTTG CAATCAAGAA GCTTTCGAAC TACCAGAAGG CGCCTCGGCG ATGGATCTTG CAAATAAGAT GAAACAATCT CATTGCTTTG CAGGGGCCCT TATTAATGAT CAAGAAAAGG ATCTTTCCAC CACCTTACAA GACGGTGATA CCGTTCTATT CCTCACTTGG GATGATCCTA AAGGTAGAGA AATCTTTTTA CACACTTCAG CACATATTCT AGCTCAAGCC GTTCTACGCT TATGGCCTTC GGCACAGCCA ACGATTGGTC CAGTCATCGA TCAAGGGTTC TACTATGATT TTGCCAATCT TTCCATCAGC GAAGAAGATT TCCCGGCTAT AGAAGCTATG GCCAAAACGA TCGCAGAAGA AAAATTCCCT ATCTCGCGTC AGGTTTTCCC AGACAAAGAA GCTGCTCTTG CCTATTTTTC TCAAAATCCC TTTAAAGCAG AGCTCATTGC TGAGCTTCCT GAAGAAGTAG AAATTTCCGC GTATACGCAA GGAGAATTCT TGGATTTATG TCGAGGCCCG CACCTTCCTT CGACAGCTCC TGTTAAAGCT TTCAAGCTAT TACGCACATC TTCTGCTTAT TGGAAAGGTG ACCCTTCTAG AGAATCGCTG ATTCGAATAT ATGGAGTCTC TTTCCCTACG ACCAAGGAAC TGAAAGAACA TCTTCATCAG CTAGAAGAAG CAAAAAAACG CGACCATCGA GTTCTTGGAA CAAAGTTAGA TCTGTTCTCT CAACAAACTT GTTCTGCTGG AATGCCTTTT TTCCATCCTC GGGGTATGGT TGTTTGGAAC GCTTTAGTTG ACTACTGGAA ACGCCTTCAT CAGCGTGCTG GCTATCAGCA AATCCAAACT CCCCAACTCA TGAATAGAGA GTTATGGGAG ATCTCTGGCC ATTGGGAAAA TTACAAAGAA AACATGTATA CCCTCACCGT AGATGAAGAA GATTATGCAA TTAAACCCAT GAATTGTCCT GGCTGCATGC TGTACTATAA AACCCAGCTA CACAGCTACC GAGAATTCCC CTTACGGATT GCAGAAATCG GCCATGTACA TAGACACGAG CTTTCTGGAG CTCTATCAGG CTTGATGCGA GTGCGTACTT TCCATCAAGA TGACGCACAC GTATTTTTAA CACCTGAGCA AGTTGAAGAG GAAACACTCA ATATCCTAAA CCTTGTTTCC GAACTCTATG GAACTTTTGG ACTCGAGTAT CACTTAGAAC TTTCCACTCG TCCTGAACAA GGAACCATTG GAAGCGATGA TCTTTGGGAA CTTGCAACAA AGGCTTTGAA AAGAGCTCTC GTTAAATCGC AAAAACCTTT TATCATAAGC CCAGGAGAAG GAGCCTTTTA CGGTCCAAAA ATCGATATCC ATGTAAAAGA TGCGATCAAC AGGACTTGGC AATGTGGAAC GATTCAGTTA GATATGTTCC TGCCGGAACG ATTCGATCTG AAGTACACAA ATGCACAAGG AGAGAAAAGT ACACCTATTA TGCTCCATAG AGCTTTGTTT GGATCTATTG AGCGATTCTT AGGCATTTTA ATCGAACATT TCAAAGGACG GTTCCCTTTG TGGCTAAGTC CAGAGCATGT TCGCATTATT ACCGTCGCAG ATCGCCACGA GGCTCGTGCC CAAGAATTGG CAAAACATTT CAGCCAAATG GGAATCATTG TCTCTGTTGA TTCATCTAAT GAGTCTGTTA GTAAGAAAAT TCGCAATGCG CAAAACATGC AAGTGAATTA CATGATCACC ATTGGAGATA AGGAATTAGA GACCCACTTA CTAGCAGTGC GCACACGAGA CAATCGTGTG TTGAACGATA TTGCTGTCGA ACAGTTCTCT CATGTTATTC TTGAAGAGCT TCGCTCTTTA TCCTTGACTC CTTCCTTATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT