ChtrB.00120.a

GTPase Obg (EC 3.6.5.-) (GTP-binding protein Obg)

CENTER ID:	ChtrB.00120.a
ORGANISM:	Chlamydia trachomatis D/UW-3/Cx
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.00120.a.B1.GE42399	full length		1	335

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChtrB.00120.a.B1.PW38655	full length		1	335

Molecular models

METHOD	RESULTS
de-novo with evolutionary constraints	ModelArchive_ma-f5ddd

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|272561.5.peg.449
UniProt:	O84423

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFVDQITLEL RAGKGGNGVV AWRKEKYLPK GGPYGGNGGN GGSILIEAVT NMYSFEEYRN LRFLKADDGQ AGASNNRTGR NGKDLVLKVP EGTLLRDAAT GELIHDFTKD GERIVVCQGG RGGKGNVFFK TSTNRAPTKA TPGKPGEIRL VELELKLIAD IGLVGFPNAG KSTLFNTLAR TEVKVGAYPF TTLHPSLGLV HQEGMLYQKP WIMADIPGII EGASQNRGLG LDFLRHIERT RLLLFVIDIS GIERHSPEQD LKILMGELLA YKEELKDKDM VIALNKIDQL LPDEREERVA LLKQQFPDQE FILLSGLTGE GVDALYDLFK SKLSE

NT Sequence

atgtttgttg atcaaattac attagagttg cgcgccggga aaggcggtaa tggtgttgta gcttggagaa aagagaaata tctccccaaa ggaggcccct acggcggtaa tggggggaat gggggctcta ttcttatcga agcagtgacg aatatgtatt catttgaaga ataccgtaat cttcgtttcc ttaaagctga tgatgggcaa gccggtgcta gtaacaatcg tacaggaaga aacggtaaag acttagtatt aaaagttcct gaggggactc tcttacggga tgcagccact ggagaattga tacacgattt caccaaggat ggtgaacgta ttgttgtctg tcagggaggc agaggaggta aggggaatgt tttctttaaa acatcgacta accgagctcc aacaaaagct actccaggta aacctggaga aattcgcttg gttgaactgg aactcaaatt aatagctgat atcggtctcg ttggattccc taacgctggt aaatcaacct tatttaacac tcttgcacgt acagaagtga aagtcggtgc ctatccgttt accacgctac atccttcatt aggtttggtt catcaagaag gaatgcttta tcaaaaacct tggatcatgg cagatattcc aggaattatt gaaggagcgt cgcaaaaccg gggattggga ttggatttcc ttcggcatat tgaacgtacg cgattactgt tattcgtaat cgatatctct ggaatagaaa gacactcacc cgaacaagat cttaaaatct taatggggga gctcttggct tacaaagaag agctaaagga taaagacatg gtcatcgctt taaataagat cgatcagcta ctgcctgatg agcgcgaaga acgtgtagct cttcttaaac agcagttccc tgatcaagaa tttattttgc tttcagggct gacaggagaa ggggtagatg cattgtatga tctcttcaag agtaaactca gtgagtaa

Details for ChtrB.00120.a.B1.GE42399

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMF VDQITLELRA GKGGNGVVAW RKEKYLPKGG PYGGNGGNGG SILIETVTNM YSFEEYRNLR FLKADDGQAG ASNNRTGRNG KDLVLKVPEG TLLRDAATGE LIHDFTKDGE RIVVCQGGRG GKGNVFFKTS TNRAPTKATP GKPGEIRLVE LELKLIADIG LVGFPNAGKS TLFNTLARTE VKVGAYPFTT LHPSLGLVHQ EGMLYQKTWI MADIPGIIEG ASQNRGLGLD FLRHIERTRL LLFVIDISGI ERHSPEQDLK ILMGELLAYK EELKDKDMVI ALNKIDQLLP DEREERVALL KQQFPDQEFI LLSGLTGEGV DALYD

Validated NT Sequence

cagatcatac aatgcatcta ccccttctcc tgtcagccct gaaagcaaaa taaattcttg atcagggaac tgctgtttaa gaagagctac acgttcttcg cgctcatcag gcagtagctg atcgatctta tttaaagcga tgaccatgtc tttatccttt agctcttctt tgtaagccaa gagctccccc attaagattt taagatcttg ttcgggtgag tgtctttcta ttccagagat atcgattacg aataacagta atcgcgtacg ttcaatatgc cgaaggaaat ccaatcccaa tccccggttt tgcgacgctc cttcaataat tcctggaata tctgccatga tccaagtttt ttgatagagc attccttctt gatgaaccaa acccaatgaa ggatgtagcg tggtaaacgg ataggcaccg actttcactt ctgtacgtgc aagagtgtta aataaggttg atttaccagc gttagggaat ccaacgagac cgatatcagc tattaatttg agttccagtt caaccaagcg aatttctcca ggtttacctg gagtagcttt tgttggagct cggttagtcg atgttttaaa gaaaacattc cccttacctc ctctgcctcc ctgacagaca acaatacgtt caccatcctt ggtgaaatcg tgtatcaatt ctccagtggc tgcatcccgt aagagagtcc cctcaggaac ttttaatact aagtctttac cgtttcttcc tgtacgatta ttactagcac cggcttgccc atcatcagct ttaaggaaac gaagattacg gtattcttca aatgaataca tattcgtcac tgtttcgata agaatagagc ccccattccc cccattaccg ccgtaggggc ctcctttggg gagatatttc tcttttctcc aagctacaac accattaccg cctttcccgg cgcgcaactc taatgtaatt tgatcaacaa acatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHMF VDQITLELRA GKGGNGVVAW RKEKYLPKGG PYGGNGGNGG SILIEAVTNM YSFEEYRNLR FLKADDGQAG ASNNRTGRNG KDLVLKVPEG TLLRDAATGE LIHDFTKDGE RIVVCQGGRG GKGNVFFKTS TNRAPTKATP GKPGEIRLVE LELKLIADIG LVGFPNAGKS TLFNTLARTE VKVGAYPFTT LHPSLGLVHQ EGMLYQKPWI MADIPGIIEG ASQNRGLGLD FLRHIERTRL LLFVIDISGI ERHSPEQDLK ILMGELLAYK EELKDKDMVI ALNKIDQLLP DEREERVALL KQQFPDQEFI LLSGLTGEGV DALYDLFKSK LSE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttg ttgatcaaat tacattagag ttgcgcgccg ggaaaggcgg taatggtgtt gtagcttgga gaaaagagaa atatctcccc aaaggaggcc cctacggcgg taatgggggg aatgggggct ctattcttat cgaagcagtg acgaatatgt attcatttga agaataccgt aatcttcgtt tccttaaagc tgatgatggg caagccggtg ctagtaacaa tcgtacagga agaaacggta aagacttagt attaaaagtt cctgagggga ctctcttacg ggatgcagcc actggagaat tgatacacga tttcaccaag gatggtgaac gtattgttgt ctgtcaggga ggcagaggag gtaaggggaa tgttttcttt aaaacatcga ctaaccgagc tccaacaaaa gctactccag gtaaacctgg agaaattcgc ttggttgaac tggaactcaa attaatagct gatatcggtc tcgttggatt ccctaacgct ggtaaatcaa ccttatttaa cactcttgca cgtacagaag tgaaagtcgg tgcctatccg tttaccacgc tacatccttc attaggtttg gttcatcaag aaggaatgct ttatcaaaaa ccttggatca tggcagatat tccaggaatt attgaaggag cgtcgcaaaa ccggggattg ggattggatt tccttcggca tattgaacgt acgcgattac tgttattcgt aatcgatatc tctggaatag aaagacactc acccgaacaa gatcttaaaa tcttaatggg ggagctcttg gcttacaaag aagagctaaa ggataaagac atggtcatcg ctttaaataa gatcgatcag ctactgcctg atgagcgcga agaacgtgta gctcttctta aacagcagtt ccctgatcaa gaatttattt tgctttcagg gctgacagga gaaggggtag atgcattgta tgatctcttc aagagtaaac tcagtgagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for ChtrB.00120.a.B1.PW38655

PURIFICATION DATe:	7/3/2019
CONCENTRATION:	12mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	8
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTTG TTGATCAAAT TACATTAGAG TTGCGCGCCG GGAAAGGCGG TAATGGTGTT GTAGCTTGGA GAAAAGAGAA ATATCTCCCC AAAGGAGGCC CCTACGGCGG TAATGGGGGG AATGGGGGCT CTATTCTTAT CGAAGCAGTG ACGAATATGT ATTCATTTGA AGAATACCGT AATCTTCGTT TCCTTAAAGC TGATGATGGG CAAGCCGGTG CTAGTAACAA TCGTACAGGA AGAAACGGTA AAGACTTAGT ATTAAAAGTT CCTGAGGGGA CTCTCTTACG GGATGCAGCC ACTGGAGAAT TGATACACGA TTTCACCAAG GATGGTGAAC GTATTGTTGT CTGTCAGGGA GGCAGAGGAG GTAAGGGGAA TGTTTTCTTT AAAACATCGA CTAACCGAGC TCCAACAAAA GCTACTCCAG GTAAACCTGG AGAAATTCGC TTGGTTGAAC TGGAACTCAA ATTAATAGCT GATATCGGTC TCGTTGGATT CCCTAACGCT GGTAAATCAA CCTTATTTAA CACTCTTGCA CGTACAGAAG TGAAAGTCGG TGCCTATCCG TTTACCACGC TACATCCTTC ATTAGGTTTG GTTCATCAAG AAGGAATGCT TTATCAAAAA CCTTGGATCA TGGCAGATAT TCCAGGAATT ATTGAAGGAG CGTCGCAAAA CCGGGGATTG GGATTGGATT TCCTTCGGCA TATTGAACGT ACGCGATTAC TGTTATTCGT AATCGATATC TCTGGAATAG AAAGACACTC ACCCGAACAA GATCTTAAAA TCTTAATGGG GGAGCTCTTG GCTTACAAAG AAGAGCTAAA GGATAAAGAC ATGGTCATCG CTTTAAATAA GATCGATCAG CTACTGCCTG ATGAGCGCGA AGAACGTGTA GCTCTTCTTA AACAGCAGTT CCCTGATCAA GAATTTATTT TGCTTTCAGG GCTGACAGGA GAAGGGGTAG ATGCATTGTA TGATCTCTTC AAGAGTAAAC TCAGTGAGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT