ChtrB.00084.a
Enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (2-phosphoglycerate dehydratase)
| CENTER ID: | ChtrB.00084.a |
| ORGANISM: | Chlamydia trachomatis D/UW-3/Cx |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ChtrB.00084.a.B1.GE42398 | full length | 1 | 424 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ChtrB.00084.a.B1.PW38531 | full length | 1 | 424 |
Structures
6O4N
DEPOSITED: 2/28/2019
DETERMINATION: XRay
CLONE: ChtrB.00084.a.B1.GE42398
PROTEIN: ChtrB.00084.a.B1.PW38531
8W21
DEPOSITED: 2/19/2024
DETERMINATION: XRay
CLONE: ChtrB.00084.a.B1.GE42398
PROTEIN: ChtrB.00084.a.B1.PW39241
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_82009 |
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|272561.5.peg.639 |
| UniProt: | O84591 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFDVVISDIE AREILDSRGY PTLCVKVITN TGTFGEACVP SGASTGIKEA LELRDKDPKR YQGKGVLQAI SNVEKVLMPA LQGFSVFDQI TADAIMIDAD GTPNKEKLGA NAILGVSLAL AKAAANTLQR PLYRYLGGSF SHVLPCPMMN LINGGMHATN GLQFQEFMIR PISAPSLTEA VRMGAEVFNA LKKILQNRQL ATGVGDEGGF APNLASNAEA LDLLLTAIET AGFTPREDIS LALDCAASSF YNTQDKTYDG KSYADQVGIL AELCEHYPID SIEDGLAEED FEGWKLLSET LGDRVQLVGD DLFVTNSALI AEGIAQGLAN AVLIKPNQIG TLTETAEAIR LATIQGYATI LSHRSGETED TTIADLAVAF NTGQIKTGSL SRSERIAKYN RLMAIEEEMG PEALFQDSNP FSKA
NT Sequence
atgtttgatg tcgtcatctc cgatatagaa gcgagagaaa ttttagattc tcgaggctat cccacattat gtgttaaagt catcactaat acaggaacct ttggtgaagc gtgcgttcct tctggagcat ctacaggcat caaggaagct ttggaactgc gtgacaaaga tcctaaacgt taccaaggga aaggggtctt acaagccatt tctaatgtcg aaaaagtgct gatgcccgct ttacaaggat tcagcgtatt tgaccaaatt acagctgatg cgattatgat tgatgctgat ggaactccga acaaagaaaa gttaggagct aatgcgattc ttggagtctc cctagcatta gcaaaagctg ctgcaaatac tttacagaga cctttatatc ggtatcttgg tggatctttc tcgcatgtgc ttccttgccc tatgatgaat cttatcaatg gcggtatgca tgctacaaat ggtctccaat tccaagaatt tatgattcgt ccaattagcg ctccttctct aacagaggct gtgcggatgg gagcagaagt cttcaacgcc ttaaaaaaaa tcttacagaa tcgacagctg gctacaggtg ttggtgatga aggcggattt gctcctaatc ttgcctctaa tgccgaagct ctggatctac tcttaacagc aatcgaaact gcaggattca cacctagaga agatatttct ttagctctcg actgcgctgc ttcttctttc tataataccc aagataaaac ctatgatggg aaatcgtatg cagatcaagt gggtatactt gcagaactct gtgagcacta tcctatagat tctatcgaag atgggctagc cgaagaagat tttgagggct ggaaactcct atccgagact ttaggagatc gtgtgcaact agttggagac gacctatttg tgacgaattc tgcattgatt gctgaaggaa tcgctcaagg acttgccaat gccgttctca tcaaaccaaa ccaaattgga acacttacag aaactgcaga agctattcgt ttagcaacta tacaaggcta cgctaccatt ctttcgcata gatcaggaga aacagaagat actaccatag cagaccttgc tgtcgctttt aatacaggtc agattaaaac agggtctctt tcccgttctg agcgtatcgc taagtataac cgtctaatgg caattgaaga agagatgggt ccagaagctc tattccaaga ttcaaatccc ttttctaaag catag
Details for ChtrB.00084.a.B1.GE42398
| HARVESTED ON: | 5/25/2018 |
| SEQUENCED ON: | 5/31/2018 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 46kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMF DVVISDIEAR EILDSRGYPT LCVKVITNTG TFGEACVPSG ASTGIKEALE LRDKDPKRYQ GKGVLQAISN VEKVLVPALQ GFSVFDQITA DAIMIDADGT PNKEKLGANA ILGVSLALAK AAANTLQRPL YRYLGGSFSH VLPCPMMNLI NGGMHATNGL QFQEFMIRPI SAPSLKEAVR MGAEVFNALK KILQNRQLAT GVGDEGGFAP NLASNAEALD LLLTAIETAG FTPREDISLA LDCAASSFYN TQDKTYDGKS YADQVGILAE LCEHYPIDSI EDGLAEEDFE GWKLLSETLG DRVQLVGDDL FVTNSALIAE GIAQGLANAV LIKPNQIGTL TETAEAIRLA TIQGYATILS HRSGETEDTT IADLAVAFNT GQIKTGSLSR SERIAKYNRL MAIEEEMGPE ALFQ
Validated NT Sequence
cttggaatag agcttctgga cccatctctt cttcaattgc cattagacgg ttatacttag cgatacgctc agaacgggaa agagaccctg ttttaatctg acctgtatta aaagcgacag caaggtctgc tatggtagta tcttctgttt ctcctgatct atgcgaaaga atggtagcgt agccttgtat agttgctaaa cgaatagctt ctgcagtttc tgtaagtgtt ccaatttggt ttggtttgat gagaacggca ttggcaagtc cttgagcgat tccttcagca atcaatgcag aattcgtcac aaataggtcg tctccaacta gttgcacacg atctcctaaa gtctcggata ggagtttcca gccctcaaaa tcttcttcgg ctagcccatc ttcgatagaa tctataggat agtgctcaca gagttctgca agtataccca cttgatctgc atacgatttc ccatcatagg ttttatcttg ggtattatag aaagaagaag cagcgcagtc gagagctaaa gaaatatctt ctctaggtgt gaatcctgca gtttcgattg ctgttaagag tagatccaga gcttcggcat tagaggcaag attaggagca aatccgcctt catcaccaac acctgtagcc agctgtcgat tctgtaagat tttttttaag gcgttgaaga cttctgctcc catccgcaca gcctctttta gagaaggagc gctaattgga cgaatcataa attcttggaa ttggagacca tttgtagcat gcataccgcc attgatgaga ttcatcatag ggcaaggaag cacatgcgag aaagatccac caagataccg atataaaggt ctctgtaaag tatttgcagc agcttttgct aatgctaggg agactccaag aatcgcatta gctcctaact tttctttgtt cggagttcca tcagcatcaa tcataatcgc atcagctgta atttggtcaa atacgctgaa tccttgtaaa gcgggcacca gcactttttc gacattagaa atggcttgta agaccccttt cccttggtaa cgtttaggat ctttgtcacg cagttccaaa gcttccttga tgcctgtaga tgctccagaa ggaacgcacg cttcaccaaa ggttcctgta ttagtgatga ctttaacaca taatgtggga tagcctcgag aatctaaaat ttctctcgct tctatatcgg agatgacgac atcaaacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMF DVVISDIEAR EILDSRGYPT LCVKVITNTG TFGEACVPSG ASTGIKEALE LRDKDPKRYQ GKGVLQAISN VEKVLMPALQ GFSVFDQITA DAIMIDADGT PNKEKLGANA ILGVSLALAK AAANTLQRPL YRYLGGSFSH VLPCPMMNLI NGGMHATNGL QFQEFMIRPI SAPSLTEAVR MGAEVFNALK KILQNRQLAT GVGDEGGFAP NLASNAEALD LLLTAIETAG FTPREDISLA LDCAASSFYN TQDKTYDGKS YADQVGILAE LCEHYPIDSI EDGLAEEDFE GWKLLSETLG DRVQLVGDDL FVTNSALIAE GIAQGLANAV LIKPNQIGTL TETAEAIRLA TIQGYATILS HRSGETEDTT IADLAVAFNT GQIKTGSLSR SERIAKYNRL MAIEEEMGPE ALFQDSNPFS KA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttg atgtcgtcat ctccgatata gaagcgagag aaattttaga ttctcgaggc tatcccacat tatgtgttaa agtcatcact aatacaggaa cctttggtga agcgtgcgtt ccttctggag catctacagg catcaaggaa gctttggaac tgcgtgacaa agatcctaaa cgttaccaag ggaaaggggt cttacaagcc atttctaatg tcgaaaaagt gctgatgccc gctttacaag gattcagcgt atttgaccaa attacagctg atgcgattat gattgatgct gatggaactc cgaacaaaga aaagttagga gctaatgcga ttcttggagt ctccctagca ttagcaaaag ctgctgcaaa tactttacag agacctttat atcggtatct tggtggatct ttctcgcatg tgcttccttg ccctatgatg aatcttatca atggcggtat gcatgctaca aatggtctcc aattccaaga atttatgatt cgtccaatta gcgctccttc tctaacagag gctgtgcgga tgggagcaga agtcttcaac gccttaaaaa aaatcttaca gaatcgacag ctggctacag gtgttggtga tgaaggcgga tttgctccta atcttgcctc taatgccgaa gctctggatc tactcttaac agcaatcgaa actgcaggat tcacacctag agaagatatt tctttagctc tcgactgcgc tgcttcttct ttctataata cccaagataa aacctatgat gggaaatcgt atgcagatca agtgggtata cttgcagaac tctgtgagca ctatcctata gattctatcg aagatgggct agccgaagaa gattttgagg gctggaaact cctatccgag actttaggag atcgtgtgca actagttgga gacgacctat ttgtgacgaa ttctgcattg attgctgaag gaatcgctca aggacttgcc aatgccgttc tcatcaaacc aaaccaaatt ggaacactta cagaaactgc agaagctatt cgtttagcaa ctatacaagg ctacgctacc attctttcgc atagatcagg agaaacagaa gatactacca tagcagacct tgctgtcgct tttaatacag gtcagattaa aacagggtct ctttcccgtt ctgagcgtat cgctaagtat aaccgtctaa tggcaattga agaagagatg ggtccagaag ctctattcca agattcaaat cccttttcta aagcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for ChtrB.00084.a.B1.PW38531
| PURIFICATION DATe: | 9/25/2018 |
| CONCENTRATION: | 13.38mg/ml |
| OBSERVED MW: | 46kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMF DVVISDIEAR EILDSRGYPT LCVKVITNTG TFGEACVPSG ASTGIKEALE LRDKDPKRYQ GKGVLQAISN VEKVLVPALQ GFSVFDQITA DAIMIDADGT PNKEKLGANA ILGVSLALAK AAANTLQRPL YRYLGGSFSH VLPCPMMNLI NGGMHATNGL QFQEFMIRPI SAPSLKEAVR MGAEVFNALK KILQNRQLAT GVGDEGGFAP NLASNAEALD LLLTAIETAG FTPREDISLA LDCAASSFYN TQDKTYDGKS YADQVGILAE LCEHYPIDSI EDGLAEEDFE GWKLLSETLG DRVQLVGDDL FVTNSALIAE GIAQGLANAV LIKPNQIGTL TETAEAIRLA TIQGYATILS HRSGETEDTT IADLAVAFNT GQIKTGSLSR SERIAKYNRL MAIEEEMGPE ALFQ
Validated NT Sequence
cttggaatag agcttctgga cccatctctt cttcaattgc cattagacgg ttatacttag cgatacgctc agaacgggaa agagaccctg ttttaatctg acctgtatta aaagcgacag caaggtctgc tatggtagta tcttctgttt ctcctgatct atgcgaaaga atggtagcgt agccttgtat agttgctaaa cgaatagctt ctgcagtttc tgtaagtgtt ccaatttggt ttggtttgat gagaacggca ttggcaagtc cttgagcgat tccttcagca atcaatgcag aattcgtcac aaataggtcg tctccaacta gttgcacacg atctcctaaa gtctcggata ggagtttcca gccctcaaaa tcttcttcgg ctagcccatc ttcgatagaa tctataggat agtgctcaca gagttctgca agtataccca cttgatctgc atacgatttc ccatcatagg ttttatcttg ggtattatag aaagaagaag cagcgcagtc gagagctaaa gaaatatctt ctctaggtgt gaatcctgca gtttcgattg ctgttaagag tagatccaga gcttcggcat tagaggcaag attaggagca aatccgcctt catcaccaac acctgtagcc agctgtcgat tctgtaagat tttttttaag gcgttgaaga cttctgctcc catccgcaca gcctctttta gagaaggagc gctaattgga cgaatcataa attcttggaa ttggagacca tttgtagcat gcataccgcc attgatgaga ttcatcatag ggcaaggaag cacatgcgag aaagatccac caagataccg atataaaggt ctctgtaaag tatttgcagc agcttttgct aatgctaggg agactccaag aatcgcatta gctcctaact tttctttgtt cggagttcca tcagcatcaa tcataatcgc atcagctgta atttggtcaa atacgctgaa tccttgtaaa gcgggcacca gcactttttc gacattagaa atggcttgta agaccccttt cccttggtaa cgtttaggat ctttgtcacg cagttccaaa gcttccttga tgcctgtaga tgctccagaa ggaacgcacg cttcaccaaa ggttcctgta ttagtgatga ctttaacaca taatgtggga tagcctcgag aatctaaaat ttctctcgct tctatatcgg agatgacgac atcaaacata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMF DVVISDIEAR EILDSRGYPT LCVKVITNTG TFGEACVPSG ASTGIKEALE LRDKDPKRYQ GKGVLQAISN VEKVLMPALQ GFSVFDQITA DAIMIDADGT PNKEKLGANA ILGVSLALAK AAANTLQRPL YRYLGGSFSH VLPCPMMNLI NGGMHATNGL QFQEFMIRPI SAPSLTEAVR MGAEVFNALK KILQNRQLAT GVGDEGGFAP NLASNAEALD LLLTAIETAG FTPREDISLA LDCAASSFYN TQDKTYDGKS YADQVGILAE LCEHYPIDSI EDGLAEEDFE GWKLLSETLG DRVQLVGDDL FVTNSALIAE GIAQGLANAV LIKPNQIGTL TETAEAIRLA TIQGYATILS HRSGETEDTT IADLAVAFNT GQIKTGSLSR SERIAKYNRL MAIEEEMGPE ALFQDSNPFS KA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTTG ATGTCGTCAT CTCCGATATA GAAGCGAGAG AAATTTTAGA TTCTCGAGGC TATCCCACAT TATGTGTTAA AGTCATCACT AATACAGGAA CCTTTGGTGA AGCGTGCGTT CCTTCTGGAG CATCTACAGG CATCAAGGAA GCTTTGGAAC TGCGTGACAA AGATCCTAAA CGTTACCAAG GGAAAGGGGT CTTACAAGCC ATTTCTAATG TCGAAAAAGT GCTGATGCCC GCTTTACAAG GATTCAGCGT ATTTGACCAA ATTACAGCTG ATGCGATTAT GATTGATGCT GATGGAACTC CGAACAAAGA AAAGTTAGGA GCTAATGCGA TTCTTGGAGT CTCCCTAGCA TTAGCAAAAG CTGCTGCAAA TACTTTACAG AGACCTTTAT ATCGGTATCT TGGTGGATCT TTCTCGCATG TGCTTCCTTG CCCTATGATG AATCTTATCA ATGGCGGTAT GCATGCTACA AATGGTCTCC AATTCCAAGA ATTTATGATT CGTCCAATTA GCGCTCCTTC TCTAACAGAG GCTGTGCGGA TGGGAGCAGA AGTCTTCAAC GCCTTAAAAA AAATCTTACA GAATCGACAG CTGGCTACAG GTGTTGGTGA TGAAGGCGGA TTTGCTCCTA ATCTTGCCTC TAATGCCGAA GCTCTGGATC TACTCTTAAC AGCAATCGAA ACTGCAGGAT TCACACCTAG AGAAGATATT TCTTTAGCTC TCGACTGCGC TGCTTCTTCT TTCTATAATA CCCAAGATAA AACCTATGAT GGGAAATCGT ATGCAGATCA AGTGGGTATA CTTGCAGAAC TCTGTGAGCA CTATCCTATA GATTCTATCG AAGATGGGCT AGCCGAAGAA GATTTTGAGG GCTGGAAACT CCTATCCGAG ACTTTAGGAG ATCGTGTGCA ACTAGTTGGA GACGACCTAT TTGTGACGAA TTCTGCATTG ATTGCTGAAG GAATCGCTCA AGGACTTGCC AATGCCGTTC TCATCAAACC AAACCAAATT GGAACACTTA CAGAAACTGC AGAAGCTATT CGTTTAGCAA CTATACAAGG CTACGCTACC ATTCTTTCGC ATAGATCAGG AGAAACAGAA GATACTACCA TAGCAGACCT TGCTGTCGCT TTTAATACAG GTCAGATTAA AACAGGGTCT CTTTCCCGTT CTGAGCGTAT CGCTAAGTAT AACCGTCTAA TGGCAATTGA AGAAGAGATG GGTCCAGAAG CTCTATTCCA AGATTCAAAT CCCTTTTCTA AAGCATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT