ChtrA.00612.a
Lysine--tRNA ligase (LysS)
| CENTER ID: | ChtrA.00612.a |
| ORGANISM: | Chlamydia trachomatis UCH-1/proctitis |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ChtrA.00612.a.B1.GE41839 | full length | 1 | 526 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ChtrA.00612.a.B1.PW38393 | full length | 1 | 526 |
Structures
6NRZ
DEPOSITED: 1/24/2019
DETERMINATION: XRay
CLONE: ChtrA.00612.a.B1.GE41839
PROTEIN: ChtrA.00612.a.B1.PW38393
6NS0
DEPOSITED: 1/24/2019
DETERMINATION: XRay
CLONE: ChtrA.00612.a.B1.GE41839
PROTEIN: ChtrA.00612.a.B1.PW38393
6O3F
DEPOSITED: 2/26/2019
DETERMINATION: XRay
CLONE: ChtrA.00612.a.B1.GE41839
PROTEIN: ChtrA.00612.a.B1.PW38393
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|471473.4.peg.162 |
| UniProt: | B0BAN6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVEVEYLQH EDYLYRTSKL KEIRDLGINP YPYQYTDCLE VQEIRNQFVD NELGDSEAAF RKETPKVRFA GRLVLFRSMG KNAFGQILDN DAKIQVMFNR DFSAVAGLAA DAGISPIKFI EKKLDLGDIL GLEGYLFFTH SGELTVLVET VTLLCKSLIS LPDKHAGLAD KEIRYRKRWA DLISSEDVRK TFLTRSRILK LIREYMDQQS FLEVETPILQ TVYGGAEATP FVTTLQALHA EMFLRISLEI ALKKLLVGGM SRVYEIGKVF RNEGIDRTHN PEFTMIEAYA AYWDYNDVMK CVENLVEYIV RALNNGETQV QYSHLKSGPQ VVDFKAPWIR MTMKESISVY GGVDVDLHAD HELRKILETQ TSLPEKTYVH ASRGELIALL FDELVCDKLI APHHITDHPL ETTPLCKTLR SGDETLVERF ESFCLGKELC NAYSELNDPL QQRKLLEEQM RKKALNPDSE YHPIDEEFLE ALCQGMPPAG GFGIGIDRLV MMLTDAASIR DVLFFPVMRR IEAKKD
NT Sequence
atgtctgtag aagttgaata cttgcaacac gaagattatt tgtatagaac aagcaagcta aaggaaatca gagatttggg cataaatcct tacccttatc aatacactga ttgtcttgaa gtacaggaaa ttcgtaatca gtttgtagat aacgaattag gagatagcga agcggctttt cgtaaagaga cacctaaggt gcgttttgcc ggacgacttg ttcttttccg ttctatgggg aaaaatgctt ttgggcagat cctcgataat gatgcaaaga ttcaagtgat gttcaatcga gatttttctg cagtggcagg gttagccgcg gatgctggga tttctccgat taaatttatt gagaagaaac ttgatctagg agacatcttg ggtctcgaag ggtatctttt ctttactcac tcaggagaat taacggttct cgttgaaacg gtaacgttgt tatgtaaatc cttaatttct ttgcctgata agcatgcagg attagcagat aaagaaattc gctatcgcaa acgttgggca gatctgattt cctcagagga tgtgcgtaag actttcttaa caagaagccg gattcttaag ttgattcgtg agtacatgga tcagcagagc tttttagagg tggaaactcc tatcttgcaa accgtctacg gaggagcaga agcaactcct tttgttacca cgctgcaagc gctacatgca gaaatgttcc taagaatttc tctagagatt gctttgaaaa aactccttgt tggaggaatg tcccgagttt atgaaatcgg caaagttttc cgtaacgaag gaatcgatag aacgcataat ccagagttta ccatgataga ggcttatgcg gcttattggg attacaatga tgtaatgaaa tgtgtggaaa accttgttga gtatatcgta cgtgctttga ataacgggga aactcaggtt cagtattcac atttaaaatc aggacctcag gttgttgatt ttaaagctcc atggatccgt atgacgatga aagaaagtat ctctgtctat ggcggcgtcg atgtagactt acatgcagat catgaattac gtaaaatttt agaaacacaa acatctcttc cagagaaaac gtatgttcat gcctcacgag gagagctgat cgctttgcta tttgatgagc tggtttgtga taagctcatc gctccgcatc acattacaga tcatccatta gaaacaacgc cactttgtaa aacattgcgt tctggggatg aaactctggt agaacgattt gagagttttt gtttaggaaa agagttgtgt aatgcttatt cggaactcaa tgatccttta caacaacgaa aattgttgga agagcaaatg cgtaaaaagg ctttaaatcc tgacagcgaa taccatccta tagatgaaga atttctagaa gctctttgcc aaggaatgcc tcctgcagga ggatttggaa taggtatcga tcgattggtt atgatgttga cagacgctgc atccattcgg gatgtcctgt ttttccctgt tatgcggcgt atagaagcaa aaaaagatta a
Details for ChtrA.00612.a.B1.GE41839
| HARVESTED ON: | 8/31/2017 |
| SEQUENCED ON: | 9/29/2017 |
| EXPECTED MW: | 61kDa |
| OBSERVED MW: | 62kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS VEVEYLQHED YLYRTSKLKE IRDLGINPYP YQYTDCLEVQ EIRNQFVDNE LGDSEAAFRK ETPKVRFAGR LVLFRSMGKN AFGQILDNDA KIQVMFNRDF SAVAGLAADA GISPIKFIEK KLDLGDILGL EGYLFFTHSG ELTVLVETVT LLCKSLISLP DKHAGLADKE IRYRKRWADL ISSEDVRKTF LTRSRILKLI REYMDQQSFL EVETPILQTV YGGAEATPFV TTLQALHAEM FLRISLEIAL KKLLVGGMSR VYEIGKVFRN EGIDRTHNPE FTMIEAYAAY WDYNDVMKCV ENLVEYIVRA LNNGETQVQY SHLKSGPQVV DFKAPWIRMT MKESISVYGG VDVDLHADHE LRKILETQTS LPEKTYVHAS RGELIALLFD ELVCDKLIAP HHITDHPLET TPLCKTLRSG DETLVERFES FCLGKELCNA YSELNDPLQQ RKLLEEQMRK KALNPDSEYH PIDEEFLEAL CQGMPPAGGF GIGIDRLVMM LTDAASIRDV LFFPVXRXIE AKKD
Validated NT Sequence
taacagggaa aaacaggaca tcccgaatgg atgcagcgtc tgtcaacatc ataaccaatc gatcgatacc tattccaaat cctcctgcag gaggcattcc ttggcaaaga gcttctagaa attcttcatc tataggatgg tattcgctgt caggatttaa agccttttta cgcatttgct cttccaacaa ttttcgttgt tgtaaaggat cattgagttc cgaataagca ttacacaact cttttcctaa acaaaaactc tcaaatcgtt ctaccagagt ttcatcccca gaacgcaatg ttttacaaag tggcgttgtt tctaatggat gatctgtaat gtgatgcgga gcgatgagct tatcacaaac cagctcatca aatagcaaag cgatcagctc tcctcgtgag gcatgaacat acgttttctc tggaagagat gtttgtgttt ctaaaatttt acgtaattca tgatctgcat gtaagtctac atcgacgccg ccatagacag agatactttc tttcatcgtc atacggatcc atggagcttt aaaatcaaca acctgaggtc ctgattttaa atgtgaatac tgaacctgag tttccccgtt attcaaagca cgtacgatat actcaacaag gttttccaca catttcatta catcattgta atcccaataa gccgcataag cctctatcat ggtaaactct ggattatgcg ttctatcgat tccttcgtta cggaaaactt tgccgatttc ataaactcgg gacattccnn cannaaggag tttttttcaa agcaatctct agagaaattc ttaggaacat ttctgcatgt agcgcttgca gcgtggtaac aaaaggagtt gcttctgctc ctccgtagac ggtttgcaag ataggagttt ccacctctaa aaagctctgc tgatccatgt actcacgaat caacttaaga atccggcttc ttgttaagaa agtcttacgc acatcctctg aggaaatcag atctgcccaa cgtttgcgat agcgaatttc tttatctgct aatcctgcat gcttatcagg caaagaaatt aaggatttac ataacaacgt taccgtttca acgagaaccg ttaattctcc tgagtgagta aagaaaagat acccttcgag acccaagatg tctcctagat caagtttctt ctcaataaat ttaatcggag aaatcccagc atccgcggct aaccctgcca ctgcagaaaa atctcgattg aacatcactt gaatctttgc atcattatcg aggatctgcc caaaagcatt tttccccata gaacggaaaa gaacaagtcg tccggcaaaa cgcaccttag gtgtctcttt acgaaaagcc gcttcgctat ctcctaattc gttatctaca aactgattac gaatttcctg tacttcaaga caatcagtgt attgataagg gtaaggattt atgcccaaat ctctgatttc ctttagcttg cttgttctat acaaataatc ttcgtgttgc aagtattcaa cttctacaga catatggtgg tggtggtggt gagccatggt at
Expected Protein Sequence
MAHHHHHHMS VEVEYLQHED YLYRTSKLKE IRDLGINPYP YQYTDCLEVQ EIRNQFVDNE LGDSEAAFRK ETPKVRFAGR LVLFRSMGKN AFGQILDNDA KIQVMFNRDF SAVAGLAADA GISPIKFIEK KLDLGDILGL EGYLFFTHSG ELTVLVETVT LLCKSLISLP DKHAGLADKE IRYRKRWADL ISSEDVRKTF LTRSRILKLI REYMDQQSFL EVETPILQTV YGGAEATPFV TTLQALHAEM FLRISLEIAL KKLLVGGMSR VYEIGKVFRN EGIDRTHNPE FTMIEAYAAY WDYNDVMKCV ENLVEYIVRA LNNGETQVQY SHLKSGPQVV DFKAPWIRMT MKESISVYGG VDVDLHADHE LRKILETQTS LPEKTYVHAS RGELIALLFD ELVCDKLIAP HHITDHPLET TPLCKTLRSG DETLVERFES FCLGKELCNA YSELNDPLQQ RKLLEEQMRK KALNPDSEYH PIDEEFLEAL CQGMPPAGGF GIGIDRLVMM LTDAASIRDV LFFPVMRRIE AKKD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg tagaagttga atacttgcaa cacgaagatt atttgtatag aacaagcaag ctaaaggaaa tcagagattt gggcataaat ccttaccctt atcaatacac tgattgtctt gaagtacagg aaattcgtaa tcagtttgta gataacgaat taggagatag cgaagcggct tttcgtaaag agacacctaa ggtgcgtttt gccggacgac ttgttctttt ccgttctatg gggaaaaatg cttttgggca gatcctcgat aatgatgcaa agattcaagt gatgttcaat cgagattttt ctgcagtggc agggttagcc gcggatgctg ggatttctcc gattaaattt attgagaaga aacttgatct aggagacatc ttgggtctcg aagggtatct tttctttact cactcaggag aattaacggt tctcgttgaa acggtaacgt tgttatgtaa atccttaatt tctttgcctg ataagcatgc aggattagca gataaagaaa ttcgctatcg caaacgttgg gcagatctga tttcctcaga ggatgtgcgt aagactttct taacaagaag ccggattctt aagttgattc gtgagtacat ggatcagcag agctttttag aggtggaaac tcctatcttg caaaccgtct acggaggagc agaagcaact ccttttgtta ccacgctgca agcgctacat gcagaaatgt tcctaagaat ttctctagag attgctttga aaaaactcct tgttggagga atgtcccgag tttatgaaat cggcaaagtt ttccgtaacg aaggaatcga tagaacgcat aatccagagt ttaccatgat agaggcttat gcggcttatt gggattacaa tgatgtaatg aaatgtgtgg aaaaccttgt tgagtatatc gtacgtgctt tgaataacgg ggaaactcag gttcagtatt cacatttaaa atcaggacct caggttgttg attttaaagc tccatggatc cgtatgacga tgaaagaaag tatctctgtc tatggcggcg tcgatgtaga cttacatgca gatcatgaat tacgtaaaat tttagaaaca caaacatctc ttccagagaa aacgtatgtt catgcctcac gaggagagct gatcgctttg ctatttgatg agctggtttg tgataagctc atcgctccgc atcacattac agatcatcca ttagaaacaa cgccactttg taaaacattg cgttctgggg atgaaactct ggtagaacga tttgagagtt tttgtttagg aaaagagttg tgtaatgctt attcggaact caatgatcct ttacaacaac gaaaattgtt ggaagagcaa atgcgtaaaa aggctttaaa tcctgacagc gaataccatc ctatagatga agaatttcta gaagctcttt gccaaggaat gcctcctgca ggaggatttg gaataggtat cgatcgattg gttatgatgt tgacagacgc tgcatccatt cgggatgtcc tgtttttccc tgttatgcgg cgtatagaag caaaaaaaga ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for ChtrA.00612.a.B1.PW38393
| PURIFICATION DATe: | 11/15/2017 |
| CONCENTRATION: | 25.76mg/ml |
| OBSERVED MW: | 61kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
UV lamp died no SEC profile
Validated AA Sequence
MAHHHHHHMS VEVEYLQHED YLYRTSKLKE IRDLGINPYP YQYTDCLEVQ EIRNQFVDNE LGDSEAAFRK ETPKVRFAGR LVLFRSMGKN AFGQILDNDA KIQVMFNRDF SAVAGLAADA GISPIKFIEK KLDLGDILGL EGYLFFTHSG ELTVLVETVT LLCKSLISLP DKHAGLADKE IRYRKRWADL ISSEDVRKTF LTRSRILKLI REYMDQQSFL EVETPILQTV YGGAEATPFV TTLQALHAEM FLRISLEIAL KKLLVGGMSR VYEIGKVFRN EGIDRTHNPE FTMIEAYAAY WDYNDVMKCV ENLVEYIVRA LNNGETQVQY SHLKSGPQVV DFKAPWIRMT MKESISVYGG VDVDLHADHE LRKILETQTS LPEKTYVHAS RGELIALLFD ELVCDKLIAP HHITDHPLET TPLCKTLRSG DETLVERFES FCLGKELCNA YSELNDPLQQ RKLLEEQMRK KALNPDSEYH PIDEEFLEAL CQGMPPAGGF GIGIDRLVMM LTDAASIRDV LFFPVXRXIE AKKD
Validated NT Sequence
taacagggaa aaacaggaca tcccgaatgg atgcagcgtc tgtcaacatc ataaccaatc gatcgatacc tattccaaat cctcctgcag gaggcattcc ttggcaaaga gcttctagaa attcttcatc tataggatgg tattcgctgt caggatttaa agccttttta cgcatttgct cttccaacaa ttttcgttgt tgtaaaggat cattgagttc cgaataagca ttacacaact cttttcctaa acaaaaactc tcaaatcgtt ctaccagagt ttcatcccca gaacgcaatg ttttacaaag tggcgttgtt tctaatggat gatctgtaat gtgatgcgga gcgatgagct tatcacaaac cagctcatca aatagcaaag cgatcagctc tcctcgtgag gcatgaacat acgttttctc tggaagagat gtttgtgttt ctaaaatttt acgtaattca tgatctgcat gtaagtctac atcgacgccg ccatagacag agatactttc tttcatcgtc atacggatcc atggagcttt aaaatcaaca acctgaggtc ctgattttaa atgtgaatac tgaacctgag tttccccgtt attcaaagca cgtacgatat actcaacaag gttttccaca catttcatta catcattgta atcccaataa gccgcataag cctctatcat ggtaaactct ggattatgcg ttctatcgat tccttcgtta cggaaaactt tgccgatttc ataaactcgg gacattccnn cannaaggag tttttttcaa agcaatctct agagaaattc ttaggaacat ttctgcatgt agcgcttgca gcgtggtaac aaaaggagtt gcttctgctc ctccgtagac ggtttgcaag ataggagttt ccacctctaa aaagctctgc tgatccatgt actcacgaat caacttaaga atccggcttc ttgttaagaa agtcttacgc acatcctctg aggaaatcag atctgcccaa cgtttgcgat agcgaatttc tttatctgct aatcctgcat gcttatcagg caaagaaatt aaggatttac ataacaacgt taccgtttca acgagaaccg ttaattctcc tgagtgagta aagaaaagat acccttcgag acccaagatg tctcctagat caagtttctt ctcaataaat ttaatcggag aaatcccagc atccgcggct aaccctgcca ctgcagaaaa atctcgattg aacatcactt gaatctttgc atcattatcg aggatctgcc caaaagcatt tttccccata gaacggaaaa gaacaagtcg tccggcaaaa cgcaccttag gtgtctcttt acgaaaagcc gcttcgctat ctcctaattc gttatctaca aactgattac gaatttcctg tacttcaaga caatcagtgt attgataagg gtaaggattt atgcccaaat ctctgatttc ctttagcttg cttgttctat acaaataatc ttcgtgttgc aagtattcaa cttctacaga catatggtgg tggtggtggt gagccatggt at
Expressed Protein Sequence
MAHHHHHHMS VEVEYLQHED YLYRTSKLKE IRDLGINPYP YQYTDCLEVQ EIRNQFVDNE LGDSEAAFRK ETPKVRFAGR LVLFRSMGKN AFGQILDNDA KIQVMFNRDF SAVAGLAADA GISPIKFIEK KLDLGDILGL EGYLFFTHSG ELTVLVETVT LLCKSLISLP DKHAGLADKE IRYRKRWADL ISSEDVRKTF LTRSRILKLI REYMDQQSFL EVETPILQTV YGGAEATPFV TTLQALHAEM FLRISLEIAL KKLLVGGMSR VYEIGKVFRN EGIDRTHNPE FTMIEAYAAY WDYNDVMKCV ENLVEYIVRA LNNGETQVQY SHLKSGPQVV DFKAPWIRMT MKESISVYGG VDVDLHADHE LRKILETQTS LPEKTYVHAS RGELIALLFD ELVCDKLIAP HHITDHPLET TPLCKTLRSG DETLVERFES FCLGKELCNA YSELNDPLQQ RKLLEEQMRK KALNPDSEYH PIDEEFLEAL CQGMPPAGGF GIGIDRLVMM LTDAASIRDV LFFPVMRRIE AKKD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTG TAGAAGTTGA ATACTTGCAA CACGAAGATT ATTTGTATAG AACAAGCAAG CTAAAGGAAA TCAGAGATTT GGGCATAAAT CCTTACCCTT ATCAATACAC TGATTGTCTT GAAGTACAGG AAATTCGTAA TCAGTTTGTA GATAACGAAT TAGGAGATAG CGAAGCGGCT TTTCGTAAAG AGACACCTAA GGTGCGTTTT GCCGGACGAC TTGTTCTTTT CCGTTCTATG GGGAAAAATG CTTTTGGGCA GATCCTCGAT AATGATGCAA AGATTCAAGT GATGTTCAAT CGAGATTTTT CTGCAGTGGC AGGGTTAGCC GCGGATGCTG GGATTTCTCC GATTAAATTT ATTGAGAAGA AACTTGATCT AGGAGACATC TTGGGTCTCG AAGGGTATCT TTTCTTTACT CACTCAGGAG AATTAACGGT TCTCGTTGAA ACGGTAACGT TGTTATGTAA ATCCTTAATT TCTTTGCCTG ATAAGCATGC AGGATTAGCA GATAAAGAAA TTCGCTATCG CAAACGTTGG GCAGATCTGA TTTCCTCAGA GGATGTGCGT AAGACTTTCT TAACAAGAAG CCGGATTCTT AAGTTGATTC GTGAGTACAT GGATCAGCAG AGCTTTTTAG AGGTGGAAAC TCCTATCTTG CAAACCGTCT ACGGAGGAGC AGAAGCAACT CCTTTTGTTA CCACGCTGCA AGCGCTACAT GCAGAAATGT TCCTAAGAAT TTCTCTAGAG ATTGCTTTGA AAAAACTCCT TGTTGGAGGA ATGTCCCGAG TTTATGAAAT CGGCAAAGTT TTCCGTAACG AAGGAATCGA TAGAACGCAT AATCCAGAGT TTACCATGAT AGAGGCTTAT GCGGCTTATT GGGATTACAA TGATGTAATG AAATGTGTGG AAAACCTTGT TGAGTATATC GTACGTGCTT TGAATAACGG GGAAACTCAG GTTCAGTATT CACATTTAAA ATCAGGACCT CAGGTTGTTG ATTTTAAAGC TCCATGGATC CGTATGACGA TGAAAGAAAG TATCTCTGTC TATGGCGGCG TCGATGTAGA CTTACATGCA GATCATGAAT TACGTAAAAT TTTAGAAACA CAAACATCTC TTCCAGAGAA AACGTATGTT CATGCCTCAC GAGGAGAGCT GATCGCTTTG CTATTTGATG AGCTGGTTTG TGATAAGCTC ATCGCTCCGC ATCACATTAC AGATCATCCA TTAGAAACAA CGCCACTTTG TAAAACATTG CGTTCTGGGG ATGAAACTCT GGTAGAACGA TTTGAGAGTT TTTGTTTAGG AAAAGAGTTG TGTAATGCTT ATTCGGAACT CAATGATCCT TTACAACAAC GAAAATTGTT GGAAGAGCAA ATGCGTAAAA AGGCTTTAAA TCCTGACAGC GAATACCATC CTATAGATGA AGAATTTCTA GAAGCTCTTT GCCAAGGAAT GCCTCCTGCA GGAGGATTTG GAATAGGTAT CGATCGATTG GTTATGATGT TGACAGACGC TGCATCCATT CGGGATGTCC TGTTTTTCCC TGTTATGCGG CGTATAGAAG CAAAAAAAGA TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT