SSGCID: Target Page

ChpsA.00362.a

UDP-N-acetylenolpyruvoylglucosamine reductase (MurB)

CENTER ID:	ChpsA.00362.a
ORGANISM:	Chlamydia psittaci MN
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChpsA.00362.a.B1.GE39365	full length		1	296

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ChpsA.00362.a.B1.PW37769	full length		1	296

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1218354.3.peg.798
RefSeq:	YP_006741936.1
UniProt:	J9WW83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKASTAIRFP FSVRRSVWLN KYSTFRIGGP ANYFKEVRSA SEAQQVIQFL HSHNYPFIIV GKGSNCLFDD RGFDGFVLCN GIQGKEFISE TTIKVYSGTS FSFLGKTLSS SGYSGLEFAV GIPGSVGGAV FMNAGIGSQD TASVIESVEV INSQGEILSY NAEELAFGYR TSRFQDCNEF ILSATFRLSR NSSSAKIAKD LLRSRLLSQP YQQPSAGCIF RNPPGNSAGK LIDEAGLKGF SLGGAQISPK HANFIVNTSR ATSQEVKQLI QMVRDKLQSQ GINLEEEVRI IPYQLP

NT Sequence

gtgaaagcat ctactgccat tcgtttcccc ttttcagttc gccgtagtgt ttggttgaat aagtattcta cgtttcgtat cggaggtcct gcgaattact ttaaagaagt gcggtcagct agcgaagcac agcaagtgat tcagttctta cacagtcaca actatccatt tattatcgtc gggaaaggtt cgaattgctt attcgatgac cgagggtttg atggttttgt tttgtgtaat ggtatacaag gaaaagaatt tatctctgaa actactatca aagtctattc gggaacttct ttttcttttc tagggaaaac tctttcttct tcaggatatt caggtttaga atttgcagta ggtattccag gatcagttgg tggggctgta tttatgaatg ctggaatcgg cagtcaagac actgcctctg taattgaaag cgtggaagtc attaattctc aaggagaaat cctttcgtat aatgctgaag aactcgcatt tggttaccgg acatcgcgtt ttcaagattg caatgaattt attctttcgg caactttccg cctatctaga aattcttcat ccgcaaaaat tgcaaaagac ttgcttcgga gtaggttact ttctcaaccc taccaacaac cttctgcggg atgcattttc cgtaatcccc caggaaattc cgcgggaaag ctcatcgatg aagctggttt aaaaggcttt tctctaggag gagcacaaat atctccaaag cacgctaatt tcattgtgaa tacgagcaga gcaacatctc aagaagtgaa acagcttatt caaatggttc gagacaagtt acaatcacaa ggaatcaact tagaagaaga agtgcgtatt attccttacc aactacccta a

Details for ChpsA.00362.a.B1.GE39365

HARVESTED ON:	7/10/2015
SEQUENCED ON:	7/6/2015
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV KASTAIRFPF SVRRSVWLNK YSTFRIGGPA NYFKEVRSAS EAQQVIQFLH SHNYPFIIVG KGSNCLFDDR GFDGFVLCNG IQGKEFISET TIKVYSGTSF SFLGKTLSSS GYSGLEFAVG IPGSVGGAVF MNAGIGSQDT ASVIESVEVI NSQGEILSHN AEELAFGYRT SRFQDCNEFI LSATFRLSRN SSSAKIAKDL LRSRLLSQPY QQPSAGCIFR NPPGNSAGKL IDEAGLKGFS LGGAQISPKH ANFIVNTGRA TSQEVKQLIQ MVRDKLQSQG INLEEEXXII PYQLP

Validated NT Sequence

atcttactca cttagggtag ttggtaagga ataatnnnnn cttcttcttc taagttgatt ccttgtgatt gtaacttgtc tcgaaccatt tgaataagct gtttcacttc ttgagatgtt gctctgcccg tattcacaat gaaattagcg tgctttggag atatttgtgc tcctcctaga gaaaagcctt ttaaaccagc ttcatcgatg agctttcccg cggaatttcc tgggggatta cggaaaatgc atcccgcaga aggttgttgg tagggttgag aaagtaacct actccgaagc aagtcttttg caatttttgc ggatgaagaa tttctagata ggcggaaagt tgccgaaaga ataaattcat tgcaatcttg aaaacgcgat gtccggtaac caaatgcgag ttcttcagca ttatgcgaaa ggatttctcc ttgagaatta atgacttcca cgctttcaat tacagaggca gtgtcttgac tgccgattcc agcattcata aatacagccc caccaactga tcctggaata cctactgcaa attctaaacc tgaatatcct gaagaagaaa gagttttccc tagaaaagaa aaagaagttc ccgaatagac tttgatagta gtttcagaga taaattcttt tccttgtata ccattacaca aaacaaaacc atcaaaccct cggtcatcga ataagcaatt cgaacctttc ccgacgataa taaatggata gttgtgactg tgtaagaact gaatcacttg ctgtgcttcg ctagctgaac gcacttcttt aaagtaattc gcaggacctc cgatacgaaa cgtagaatac ttattcaacc aaacactacg gcgaactgaa aaggggaaac gaatggcagt agatgctttc accatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHMK ASTAIRFPFS VRRSVWLNKY STFRIGGPAN YFKEVRSASE AQQVIQFLHS HNYPFIIVGK GSNCLFDDRG FDGFVLCNGI QGKEFISETT IKVYSGTSFS FLGKTLSSSG YSGLEFAVGI PGSVGGAVFM NAGIGSQDTA SVIESVEVIN SQGEILSYNA EELAFGYRTS RFQDCNEFIL SATFRLSRNS SSAKIAKDLL RSRLLSQPYQ QPSAGCIFRN PPGNSAGKLI DEAGLKGFSL GGAQISPKHA NFIVNTSRAT SQEVKQLIQM VRDKLQSQGI NLEEEVRIIP YQLP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaaag catctactgc cattcgtttc cccttttcag ttcgccgtag tgtttggttg aataagtatt ctacgtttcg tatcggaggt cctgcgaatt actttaaaga agtgcggtca gctagcgaag cacagcaagt gattcagttc ttacacagtc acaactatcc atttattatc gtcgggaaag gttcgaattg cttattcgat gaccgagggt ttgatggttt tgttttgtgt aatggtatac aaggaaaaga atttatctct gaaactacta tcaaagtcta ttcgggaact tctttttctt ttctagggaa aactctttct tcttcaggat attcaggttt agaatttgca gtaggtattc caggatcagt tggtggggct gtatttatga atgctggaat cggcagtcaa gacactgcct ctgtaattga aagcgtggaa gtcattaatt ctcaaggaga aatcctttcg tataatgctg aagaactcgc atttggttac cggacatcgc gttttcaaga ttgcaatgaa tttattcttt cggcaacttt ccgcctatct agaaattctt catccgcaaa aattgcaaaa gacttgcttc ggagtaggtt actttctcaa ccctaccaac aaccttctgc gggatgcatt ttccgtaatc ccccaggaaa ttccgcggga aagctcatcg atgaagctgg tttaaaaggc ttttctctag gaggagcaca aatatctcca aagcacgcta atttcattgt gaatacgagc agagcaacat ctcaagaagt gaaacagctt attcaaatgg ttcgagacaa gttacaatca caaggaatca acttagaaga agaagtgcgt attattcctt accaactacc ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for ChpsA.00362.a.B1.PW37769

PURIFICATION DATe:	8/12/2015
CONCENTRATION:	40mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	15
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGAAAG CATCTACTGC CATTCGTTTC CCCTTTTCAG TTCGCCGTAG TGTTTGGTTG AATAAGTATT CTACGTTTCG TATCGGAGGT CCTGCGAATT ACTTTAAAGA AGTGCGGTCA GCTAGCGAAG CACAGCAAGT GATTCAGTTC TTACACAGTC ACAACTATCC ATTTATTATC GTCGGGAAAG GTTCGAATTG CTTATTCGAT GACCGAGGGT TTGATGGTTT TGTTTTGTGT AATGGTATAC AAGGAAAAGA ATTTATCTCT GAAACTACTA TCAAAGTCTA TTCGGGAACT TCTTTTTCTT TTCTAGGGAA AACTCTTTCT TCTTCAGGAT ATTCAGGTTT AGAATTTGCA GTAGGTATTC CAGGATCAGT TGGTGGGGCT GTATTTATGA ATGCTGGAAT CGGCAGTCAA GACACTGCCT CTGTAATTGA AAGCGTGGAA GTCATTAATT CTCAAGGAGA AATCCTTTCG TATAATGCTG AAGAACTCGC ATTTGGTTAC CGGACATCGC GTTTTCAAGA TTGCAATGAA TTTATTCTTT CGGCAACTTT CCGCCTATCT AGAAATTCTT CATCCGCAAA AATTGCAAAA GACTTGCTTC GGAGTAGGTT ACTTTCTCAA CCCTACCAAC AACCTTCTGC GGGATGCATT TTCCGTAATC CCCCAGGAAA TTCCGCGGGA AAGCTCATCG ATGAAGCTGG TTTAAAAGGC TTTTCTCTAG GAGGAGCACA AATATCTCCA AAGCACGCTA ATTTCATTGT GAATACGAGC AGAGCAACAT CTCAAGAAGT GAAACAGCTT ATTCAAATGG TTCGAGACAA GTTACAATCA CAAGGAATCA ACTTAGAAGA AGAAGTGCGT ATTATTCCTT ACCAACTACC CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT