ChpsA.00137.a

UDP-N-acetylmuramate--alanine ligase (MurC)

CENTER ID: ChpsA.00137.a
ORGANISM: Chlamydia psittaci MN
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChpsA.00137.a.B2.GE39364 soluble domain 26 810
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1218354.3.peg.883
RefSeq: YP_006742022.1
UniProt: J9WZC6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRKIHYHFI GIGGIGMSAL AHILLDRGYS VSGSDLNRGA IIDKLVAKGA RYLQGHDKHH IPEDCTIIYG SGIAKDNVEY LEAVRKQLPI LHRSELLAFL MQEQTSILVS GSHGKTTVSS LITAIFQTAK KDPSYAIGGL NSLSLNGYSG SSEYFIAEAD ESDGSLKHYL PKVAVVTNLD NEHLSNFEGS KEKLALTIEE FTRKVEDPRL CFYNGDCQEL KGRISGTSYG FSQDCNLRIH SYYQDGWRSL FSLSFLGKDY LDIDLNLIGK HNIANAAVAM GIALTLGIDE ESIREALKNF SGIQRRMERK NVSEKFLFFE DYAHHPSEIS CTLRGLRDAV GLRRIVAICQ PHRFSRLQYC LEDFYKAFQD ADEVILTDVY SAGETALDLP SPEKLAETIS LSSHVQCSYV PYDNIIPYLQ QEIRVHDVCI SLGAGNIYTV GNALKDFEPR KLAVGVVCGG KSCEHDISLL SARNVVRYLS PEYYDVQYFI IDRQGLWSKV STIECEDYEG KGHYVLSSEI TEALTHVDFI LPILHGPFGE DGTLQGFLEI INKPYAGPSL LFSSVTMDKI MTKRLAASVG VPVVPYQPLT LHAWKRTPEL CMHKISEAFT FPMFVKTAHL GSSIGVFEVH NEEELKAKIS EAFLYDTDIF IEESRLGSRE IEVSCLGDSS SCYYISEPHE RCGEKRFISY EEKYGLNGRA STQIQYDLDL SEESKIRVKE FTERVYRVIQ GQGSCRVDFF LDDEGNFWLS EMNPIPGMTQ ASPFLHDFAR LGWTFEQIIH QLIISGLHKF DRKKKVGKTL NKQCLLTAKS
NT Sequence
atggatagga aaatccacta tcattttata ggtatcggcg gaataggaat gagtgcacta gctcatattt tattagaccg gggatattcc gtatcaggaa gtgatttaaa tcgaggagcc attatcgata agcttgtggc caaaggggca cggtatttac aagggcatga taaacatcat attcctgaag attgcactat catctatgga tcagggatcg ctaaggataa cgtagaatat ctagaagctg taaggaaaca gttacccata ctccatcgtt ctgaacttct cgcttttttg atgcaagaac aaacaagtat tttagtttca gggagtcatg gcaaaacgac agtatcttcg ttaatcaccg ccattttcca aacagcaaaa aaagatcctt cctatgctat tggagggctg aattctctat ctttgaatgg ttattcagga agctcagagt actttatagc ggaagctgat gaaagcgacg gctctttaaa acactatctt cctaaagttg ctgttgtaac caatttagat aatgaacatc tcagtaattt tgaagggagt aaggagaaac ttgctttaac aattgaagaa ttcactcgta aggttgaaga tccgcgttta tgtttttata atggcgattg ccaagaactt aaaggaagaa tctcaggaac atcttatgga ttttctcaag attgtaactt acgtatacat tcctattatc aagatggctg gcgctcgcta ttttctctgt ctttcttagg gaaagattac ttggacatag atcttaacct tatcggtaag cacaacattg caaacgctgc tgtagctatg ggtatagcat taactttggg tattgatgag gaaagtattc gagaggctct taagaacttt tctggtattc aaagacgtat ggaaagaaag aatgtttcag agaaattctt attctttgaa gactatgcac accatccttc tgagatctct tgcactctaa gaggattacg agatgctgtt ggcttgcgtc gtatagttgc gatatgtcaa ccacatagat tttctagatt gcaatattgt ctggaagatt tttataaagc ttttcaagat gctgatgagg taattctcac tgatgtctac agtgctggag aaactgcatt agatttaccg tctcctgaga aattagcaga gacgatttcc ttatcctctc atgtacaatg tagctatgtt ccttatgaca acattatccc atacctgcaa caagaaatac gcgttcatga tgtgtgtata tctttaggag ctggaaatat ttataccgta ggcaatgctt taaaggattt tgaacctagg aagctcgcag ttggagttgt ctgtggaggg aagtcttgcg aacacgatat ttctttgttg tctgcgagaa atgtcgttcg gtatttgtct cctgaatact acgatgtgca atactttatt atcgatcgcc agggattatg gtcaaaagtc tccactattg aatgtgaaga ttatgaaggg aaaggtcatt acgtcttgtc ttcagagata acagaggctc ttacacatgt agattttatc cttccgatat tacatggccc ttttggagag gacggtacgc ttcagggatt tttagaaatt atcaataagc cctatgctgg accctctctg ctcttttcct cagtcactat ggataagatt atgacaaaac gccttgcagc ttctgtaggt gttcccgtgg ttccttatca gccattgact ttacatgctt ggaaaagaac tccagaatta tgtatgcata agatttcaga ggcctttact tttcctatgt ttgtgaaaac tgcacatttg ggatcgagta ttggggtttt tgaagtgcat aatgaagaag aactaaaagc aaaaatatct gaagctttcc tatatgatac agatattttt atcgaagaaa gtcgtttagg atctcgcgaa attgaagtgt cttgtctcgg agactccagt agctgttact atatttctga accacatgaa cgttgtggtg agaaaagatt tattagttat gaagagaaat atggattaaa cggtagagca agcactcaaa tacaatacga tttggatctc tccgaagaat ctaaaataag agtcaaggag ttcacagaac gtgtgtaccg tgtgattcaa ggacaaggtt catgtagagt cgatttcttt ttagatgatg aaggaaattt ttggctttct gaaatgaacc ctattccagg aatgacacag gcaagtcctt ttcttcatga ttttgcccgt ttaggatgga cttttgaaca aatcattcat caattgatta tatcagggct acataagttt gatcggaaga aaaaggtcgg aaagactttg aataagcagt gtctattaac tgcgaagagc taa
Details for ChpsA.00137.a.B2.GE39364
HARVESTED ON: 7/10/2015
SEQUENCED ON: 7/6/2015
EXPECTED MW: 89kDa
OBSERVED MW: 89kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHDR GYSVSGSDLN RGAIIDKLVA KGARYLQGHD KHHIPEDCTI IYGSGIAKDN VEYLEAVRKQ LPILHRSELL AFLMQEQTSI LVSGSHGKTT VSSLITAIFQ TAKKDPSYAI GGLNSLSLNG YSGSSEYFIA EADESDGSLK HYLPKVAVVT NLDNEHLSNF EGSKEKLALT IEEFTRKVED PRLCFYNGDC QELKGRISGT SYGFSQDCNL RIHSYYQDGW RSLFSLSFLG KDYLDIDLNL IGKHNIANAA VAMGIALTLG IDEESIREAL KNFSGIQRRM ERKNVSEKFL FFEDYAHHPS EISCTLRGLR DAVGLRRIVA ICQPHRFSRL QYCLEDFYKA FQDADEVILT DVYSAGETAL DLPSPEKLAE TISLSSHVQC SYVPYDNIIP YLQQEIRVHD VCISLGAGNI YTVGNALKDF EPRKLAVGVV CGGKSCEHDI SLLSARNVVR YLSPEYYDVQ YFIIDRQGLW SKVSTIECED YEGKGHYVLS SEITEALTHV DFILPILHGP FGEDGTLQGF LEIINKPYAG PSLLFSSVTM DKIMTKRLAA SVGVPVVPYQ PLTLHAWKRT PELCMHKISE AFTFPMFVKT AHLGSSIGVF EVHNEEELKA KISEAFLYDT DIFIEESRLG SREIEVSCLG DSSSCYYISE PHERCGEKRF ISYEEKYGLN GRASTQIQYD LDLSEESKIR VKEFTERVYR VIQGQGSCRV DFFLDDEGNF WLSEMNPIPG MTQASPFLHD FARLGWTFEQ IIHQLIISGL HKFDRKKKVG KTLNKQCLLT AKS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaccggg gatattccgt atcaggaagt gatttaaatc gaggagccat tatcgataag cttgtggcca aaggggcacg gtatttacaa gggcatgata aacatcatat tcctgaagat tgcactatca tctatggatc agggatcgct aaggataacg tagaatatct agaagctgta aggaaacagt tacccatact ccatcgttct gaacttctcg cttttttgat gcaagaacaa acaagtattt tagtttcagg gagtcatggc aaaacgacag tatcttcgtt aatcaccgcc attttccaaa cagcaaaaaa agatccttcc tatgctattg gagggctgaa ttctctatct ttgaatggtt attcaggaag ctcagagtac tttatagcgg aagctgatga aagcgacggc tctttaaaac actatcttcc taaagttgct gttgtaacca atttagataa tgaacatctc agtaattttg aagggagtaa ggagaaactt gctttaacaa ttgaagaatt cactcgtaag gttgaagatc cgcgtttatg tttttataat ggcgattgcc aagaacttaa aggaagaatc tcaggaacat cttatggatt ttctcaagat tgtaacttac gtatacattc ctattatcaa gatggctggc gctcgctatt ttctctgtct ttcttaggga aagattactt ggacatagat cttaacctta tcggtaagca caacattgca aacgctgctg tagctatggg tatagcatta actttgggta ttgatgagga aagtattcga gaggctctta agaacttttc tggtattcaa agacgtatgg aaagaaagaa tgtttcagag aaattcttat tctttgaaga ctatgcacac catccttctg agatctcttg cactctaaga ggattacgag atgctgttgg cttgcgtcgt atagttgcga tatgtcaacc acatagattt tctagattgc aatattgtct ggaagatttt tataaagctt ttcaagatgc tgatgaggta attctcactg atgtctacag tgctggagaa actgcattag atttaccgtc tcctgagaaa ttagcagaga cgatttcctt atcctctcat gtacaatgta gctatgttcc ttatgacaac attatcccat acctgcaaca agaaatacgc gttcatgatg tgtgtatatc tttaggagct ggaaatattt ataccgtagg caatgcttta aaggattttg aacctaggaa gctcgcagtt ggagttgtct gtggagggaa gtcttgcgaa cacgatattt ctttgttgtc tgcgagaaat gtcgttcggt atttgtctcc tgaatactac gatgtgcaat actttattat cgatcgccag ggattatggt caaaagtctc cactattgaa tgtgaagatt atgaagggaa aggtcattac gtcttgtctt cagagataac agaggctctt acacatgtag attttatcct tccgatatta catggccctt ttggagagga cggtacgctt cagggatttt tagaaattat caataagccc tatgctggac cctctctgct cttttcctca gtcactatgg ataagattat gacaaaacgc cttgcagctt ctgtaggtgt tcccgtggtt ccttatcagc cattgacttt acatgcttgg aaaagaactc cagaattatg tatgcataag atttcagagg cctttacttt tcctatgttt gtgaaaactg cacatttggg atcgagtatt ggggtttttg aagtgcataa tgaagaagaa ctaaaagcaa aaatatctga agctttccta tatgatacag atatttttat cgaagaaagt cgtttaggat ctcgcgaaat tgaagtgtct tgtctcggag actccagtag ctgttactat atttctgaac cacatgaacg ttgtggtgag aaaagattta ttagttatga agagaaatat ggattaaacg gtagagcaag cactcaaata caatacgatt tggatctctc cgaagaatct aaaataagag tcaaggagtt cacagaacgt gtgtaccgtg tgattcaagg acaaggttca tgtagagtcg atttcttttt agatgatgaa ggaaattttt ggctttctga aatgaaccct attccaggaa tgacacaggc aagtcctttt cttcatgatt ttgcccgttt aggatggact tttgaacaaa tcattcatca attgattata tcagggctac ataagtttga tcggaagaaa aaggtcggaa agactttgaa taagcagtgt ctattaactg cgaagagctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat