ChmuA.19921.a

Uncharacterized protein TC_0424

CENTER ID: ChmuA.19921.a
ORGANISM: Chlamydia muridarum MoPn/Nigg
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChmuA.19921.a.B3.GE42249 soluble domain #2 901 1436
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ChmuA.19921.a.B3.PW38520 soluble domain #2 901 1436

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243161.6.peg.449
UniProt: Q9PKP1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNPAATLST SPDLSLKERL DASGQLCTQA GQGSPQPLSL EARRLNSNFS MRRDLVDVVE KAIESTKGNE LKKLTIYEIA LKVCTVIGAA ILFAIPLCML LGVPLWIPVV ICIGAGITFS IVKGCLRRRC QQIRQEYRAL HLYHRYLLSN KDSIDGTFLS RFDVRVRKPE EKLHGVDSEK RGDNHPIAAD RKYDFAGLAH QRYQVDAAIG IPSVQNSFWR SAAQQVKVVK DEIISGEKTS EDLRLISKQA LQVAGIDFSG AAGKESVLDV AKSLASLLAF GAQVGKDAQK STQQYHQRFF GSPLLATWCG ADLSLAAQDF VAQGKNFLDV SSRHYATLQI AIEKVQLVPV LCKIRGWKDR IRSLAQQKTL TAALLRKLYC EIEDAMHEIC IEDGISPYIQ DQVRIVTQKC LREELKDLLG KTDEELKPCD LSKIQRSVCL FATSVVSLLE GRMGVSEKSS IKEIEETVYR ELGSTILQLG GLSGGITPLI DNVHKAIRQG RALSNELRQS IQLHPERRFH RLQAKVEKLQ GFIRDPKWGA SAVHLSSQET LEQKRQLLDK LTGIQTLLSD WETRYSVFKE TKLTHIVMED FFKETEKFLN SHSAVAESCS LDCSVEELKD CDQALNADLG NIEKVMNPAD VESAEREFKQ LISDLAGVQE QLDQLSVPIC EEGVSGKRLL LNTLLSHPDT LQKKELEKRA ALEAFTSGED PEFPVRKEET LDMVSSGYDY LSNLLGKINS FESILEESRD KKISSQYMQQ LMGLANEISY ELSSSRKDPI DNLLCRLEGL STSIPLEESV EVSSSPVAEK IARASYQRIN RVEKSKVSKT LKAFTHLIKE LRRSLRKAML SKAVVAAVLS IAFSCLAIAL FSVQLTWLPI AFCVIALVLE AIPAALSIWV DKENWKVEVA SLAKELSSDK RKLPYPEMDV QNIRKLKGLK DSYGLDGVSE LRVAEAALLG AKKLPEEQKQ DTLQSTIKAL RADLKVLNKK FKRLPESYQP KKSSEAVSAK RSNGNAASVE KELAALEEKQ KECHAACLQF ETESSRFLAE RNRAKLFESK IAQQRKEREE LGNQEARYTQ VISCLEAFIA RKGVSAQHKS KEDLSLKITE LLSLNNQSLK KRDCNPLSRM QGRFQAFAEE GCLQDVKGLL ELNMCLGSAE FSLYRDEQNR MSSERFPFQN SQQLLQYGKN LFDSYDKGDR TPLLQFILGS GWGAIREACF ELKSLNKRKQ KGSSFAPEDY ENACKALKRF LKARESLRFK LSLPLGGNSE LEIGLQHHIR NQQWTKDRLT GRHQECCRDI LQHLEDWITK TRNESQECQK VETEIQNFCQ KEGSCKNLQD SVKILFSNLQ ADLNKIPQDI LRAVLRSLSL KLCYIMDKKL ECEKLEEKFA KVDAVVKIKE SEFEEHGEKW FDQYQLLQTQ MKQLELQKEK LLAEKN
NT Sequence
atgacaaatc cggctgcaac tttatcgaca agtccggacc tttctttgaa agagagactg gacgcttctg gtcagctatg cactcaagct ggtcaaggga gtcctcagcc tctgagctta gaagctcgac gcttgaattc taacttctct atgcgacggg atttggtcga tgttgtagaa aaagctattg aaagcaccaa ggggaatgag ctcaagaaac ttactatata tgaaattgct ctaaaagttt gtacggtgat cggggctgcg attcttttcg ctattcctct ttgtatgttg cttggagttc ctttatggat ccctgttgtt atttgtatag gtgcaggaat tacgttcagt attgttaagg gctgtttacg aagaaggtgt cagcagatcc gacaagaata tcgcgctctg catctgtatc atcgttatct gctttctaat aaagactcta tcgacgggac ttttttgagt cgtttcgatg ttcgtgttcg aaagcctgag gaaaaattac acggagtaga ttctgaaaaa cgcggagata atcatccaat agctgcggat cgaaaatatg attttgctgg attagctcat caacgttatc aggtagatgc cgctattgga ataccatctg ttcaaaatag tttttggaga tctgcagctc aacaagtgaa agttgttaaa gatgagatta tctcagggga aaagactagt gaagatctac gtcttatatc taaacaagct ttgcaagtag ctggtataga tttttctgga gcagcaggaa aagagtccgt actagatgta gctaaatctt tagcaagttt attagccttt ggggctcaag ttgggaaaga tgcccaaaaa tctacacagc aatatcatca gcgatttttt ggcagtcctc ttcttgcaac atggtgtggg gcagatcttt ctttggcagc tcaagatttt gttgcgcaag gaaagaattt tttagacgtt tctagccgtc attatgcgac gcttcaaatt gctattgaaa aggtacagtt ggtgcccgtg ttatgtaaga tccggggatg gaaagatagg attcgttccc tagctcaaca aaaaacactc acggcagctc ttttacgcaa gttgtattgt gaaatcgaag atgctatgca tgagatctgt attgaagatg ggatttctcc ttatatacag gatcaggtgc ggattgtaac gcaaaagtgt ttgcgagaag agttaaaaga tcttcttggg aaaacagatg aagaactgaa accatgtgat ctttctaaga tacagagaag cgtctgtttg tttgcaactt ccgttgtttc tcttttagag ggtcgaatgg gggtttcaga aaaaagttcg ataaaagaaa tagaagaaac cgtttatcgg gagttaggca gtaccatttt acaattgggg ggattatctg gtggaattac cccattgatt gacaatgtgc ataaagctat tagacaggga agagctctaa gtaatgaact ccgccaatca atacagttac acccagagcg acgtttccat cgcttgcagg ctaaggtaga aaaattacaa ggatttatcc gcgatccaaa gtggggggct tccgcagttc acctttcttc tcaagagacg ttagagcaaa aacgccagtt gcttgacaaa ttgactggaa tccaaacgct cttatcagat tgggagacac gttacagtgt gtttaaagag acgaaattga ctcacattgt aatggaagac ttcttcaaag aaaccgaaaa atttttaaat tctcactctg ctgtagcaga gtcatgttct ttggattgtt ctgtcgaaga gctgaaggac tgcgatcagg ctttgaacgc agatttagga aatatagaga aggttatgaa tccagcagat gtagaatctg cggagaggga atttaagcag ttaatatcag atctagctgg cgtacaagag cagctagacc agctttctgt ccctatttgt gaagaagggg tgagtggtaa acgtctctta ttgaatacac tgctctccca tccagatact ctacagaaga aagagttaga aaaaagggct gctttagaag ctttcacttc gggagaggat ccagagtttc ctgtcagaaa agaggaaact ttagatatgg tttctagtgg atatgactat ctttctaatc ttttaggtaa gatcaactcc tttgaatcta ttttggaaga atctagagat aagaagatct cttcccaata tatgcagcag ttaatgggct tagcgaatga aatttcttat gagttatctt cgtcccgaaa agatccgata gacaacctat tgtgtcgatt agaagggtta agtacatcga ttcctctaga agagagtgtt gaagtttcct catcccctgt ggcagaaaaa attgcgcggg cttcttatca gcgaattaat agggtggaga aaagcaaagt ttccaaaacg ttaaaggctt ttacacacct tattaaggaa ttgcgaagat ctttacgtaa agcaatgctc tctaaagctg tagtggctgc ggtcctttct atcgcctttt cttgcctagc cattgcgcta ttttctgtac agctgacttg gttgcctatc gctttttgtg tcattgcctt agtgctggag gctatccctg ctgcattgtc tatttgggta gataaagaga actggaaggt agaagttgct tctcttgcga aggagttgtc ttcagacaaa aggaagcttc cttatccaga gatggatgtt caaaatatca gaaagttgaa aggccttaaa gactcctatg ggttagatgg tgtttctgaa ttgcgtgtag cggaagcagc tttattggga gcaaaaaaac ttccggaaga gcaaaagcag gatactttgc agagtacgat aaaagcattg agagccgatt tgaaggttct taacaagaag tttaaaaggc ttccggaatc ctatcagcct aaaaaatcgt cagaagctgt ctcagcgaaa cgaagcaatg gaaacgctgc ttcagtagaa aaagagctag ctgctctaga agaaaaacaa aaagagtgcc atgctgcctg cttacaattt gaaactgaaa gttcgagatt tttggcagaa cggaatagag caaaattgtt tgagtctaaa atagctcaac aacgtaaaga aagggaggag ctagggaatc aggaagcgcg ctatactcag gtaatcagtt gtttagaagc gtttattgca agaaaaggcg tatctgctca gcacaagtct aaagaagatc tttctctcaa aataactgag ttgttgtctc ttaacaatca gagtctcaaa aaacgagatt gtaacccgct ctctaggatg caaggacgat ttcaagcatt tgctgaagaa gggtgcttac aagacgtcaa gggattgttg gaattgaata tgtgtttagg ttccgccgaa ttttctctgt atcgtgatga acaaaatcga atgagttccg agagatttcc cttccagaat tcgcagcaac ttcttcagta cggtaaaaat cttttcgatt cttatgataa aggcgatcga actccacttt tgcagtttat tttaggatct ggatgggggg cgattcgtga agcttgcttc gagctgaaat ctttaaataa acgtaagcaa aaaggctctt cttttgctcc tgaagattat gagaacgctt gtaaagcttt aaagaggttc cttaaagcac gagaaagtct acgttttaaa cttagcctcc ctttaggggg aaatagtgag ttagagattg gattgcaaca tcacatacgg aatcagcaat ggacaaagga tcgtttaaca ggtagacacc aagagtgttg tcgagatatt ttgcagcatt tagaagattg gattacgaaa actcgaaacg agtcccaaga atgccaaaaa gtagaaacgg aaatacaaaa cttttgccaa aaagaaggat cctgcaaaaa tttacaagat agtgtgaaga ttctattttc taatttacag gcagatctga acaaaattcc tcaggatatt cttcgagctg tcttacgttc tctatccttg aagctttgtt atattatgga caagaaatta gaatgtgaga agttggagga aaaatttgct aaggttgacg ctgttgttaa aataaaagag tctgagttcg aggagcatgg agagaagtgg tttgatcagt atcaactgtt acaaactcaa atgaaacagc tagagctaca aaaagagaaa ttattggcgg agaaaaacta a
Details for ChmuA.19921.a.B3.GE42249
HARVESTED ON: 2/23/2018
SEQUENCED ON: 3/1/2018
EXPECTED MW: 63kDa
OBSERVED MW: 62kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 90
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMD KENWKVEVAS LAKELSSDKR KLPYPEMDVQ NIRKLKGLKD SYGLDGVSEL RVAEAALLGA KKLPEEQKQD TLQSTIKALR ADLKVLNKKF KRLPESYQPK KSSEAVSAKR SNGNAASVEK ELAALEEKQK ECHAACLQFE TESSRFLAER NRAKLFESKI AQQRKEREEL GNQEARYTQV ISCLEAFIAR KGVSAQHKSK EDLSLKITEX XSLNNQSLKK RDCNPLXXXQ GXFQAFAEEG XLQXXXXXXX LXXXXXXXXX XXXXXXXNRX XXXXXXXXXX XXXLQXXXNL FDSYDKGDRT PLLXFXLGSG WGAXREACFE LKSLNKRKQK GSSFAPEDYE NACKALKRFL KARESLRFKL SLPLGGNSEL EIGLQHHIRN QQWTKDRLTG RHQECCRDIL QHLEDWITKT RNESQECQKV ETEIQNFCQK EGSCKNLQDS VKILFSNLQA DLNKIPQDIL RAVLRSLSLK LCYIMDKKLE CEKLEEKFAK VDAVVKIKES EFEEHGEKWF DQYQLLQTQM KQLELQKE
Validated NT Sequence
ttctcttttt gtagctctag ctgtttcatt tgagtttgta acagttgata ctgatcaaac cacttctctc catgctcctc gaactcagac tcttttattt taacaacagc gtcaacctta gcaaattttt cctccaactt ctcacattct aatttcttgt ccataatata acaaagcttc aaggatagag aacgtaagac agctcgaaga atatcctgag gaattttgtt cagatctgcc tgtaaattag aaaatagaat cttcacacta tcttgtaaat ttttgcagga tccttctttt tggcaaaagt tttgtatttc cgtttctact ttttggcatt cttgggactc gtttcgagtt ttcgtaatcc aatcttctaa atgctgcaaa atatctcgac aacactcttg gtgtctacct gttaaacgat cctttgtcca ttgctgattc cgtatgtgat gttgcaatcc aatctctaac tcactatttc cccctaaagg gaggctaagt ttaaaacgta gactttctcg tgctttaagg aacctcttta aagctttaca agcgttctca taatcttcag gagcaaaaga agagcctttt tgcttacgtt tatttaaaga tttcagctcg aagcaagctt cacgantcgc cccccatcca gatcctaaan naaantgcaa aagtggagtt cgatcgcctt tatcataaga atcgaaaaga tttnnnncnt actgaagann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntn cgattttnnt nnnnnngnnn nnnnnnnnat tnnnnnnnnn nnannnnnnn nntcaannnn nncannnnnn nnnnnnnttg taannnccct tcttcagcaa atgcttgaaa nnntccttgc nnnnnnnaga gcgggttaca atctcgtttt ttgagactct gattgttaag agannnnnac tcagttattt tgagagaaag atcttcttta gacttgtgct gagcagatac gccttttctt gcaataaacg cttctaaaca actgattacc tgagtatagc gcgcttcctg attccctagc tcctcccttt ctttacgttg ttgagctatt ttagactcaa acaattttgc tctattccgt tctgccaaaa atctcgaact ttcagtttca aattgtaagc aggcagcatg gcactctttt tgtttttctt ctagagcagc tagctctttt tctactgaag cagcgtttcc attgcttcgt ttcgctgaga cagcttctga cgatttttta ggctgatagg attccggaag ccttttaaac ttcttgttaa gaaccttcaa atcggctctc aatgctttta tcgtactctg caaagtatcc tgcttttgct cttccggaag tttttttgct cccaataaag ctgcttccgc tacacgcaat tcagaaacac catctaaccc ataggagtct ttaaggcctt tcaactttct gatattttga acatccatct ctggataagg aagcttcctt ttgtctgaag acaactcctt cgcaagagaa gcaacttcta ccttccagtt ctctttatcc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHDK ENWKVEVASL AKELSSDKRK LPYPEMDVQN IRKLKGLKDS YGLDGVSELR VAEAALLGAK KLPEEQKQDT LQSTIKALRA DLKVLNKKFK RLPESYQPKK SSEAVSAKRS NGNAASVEKE LAALEEKQKE CHAACLQFET ESSRFLAERN RAKLFESKIA QQRKEREELG NQEARYTQVI SCLEAFIARK GVSAQHKSKE DLSLKITELL SLNNQSLKKR DCNPLSRMQG RFQAFAEEGC LQDVKGLLEL NMCLGSAEFS LYRDEQNRMS SERFPFQNSQ QLLQYGKNLF DSYDKGDRTP LLQFILGSGW GAIREACFEL KSLNKRKQKG SSFAPEDYEN ACKALKRFLK ARESLRFKLS LPLGGNSELE IGLQHHIRNQ QWTKDRLTGR HQECCRDILQ HLEDWITKTR NESQECQKVE TEIQNFCQKE GSCKNLQDSV KILFSNLQAD LNKIPQDILR AVLRSLSLKL CYIMDKKLEC EKLEEKFAKV DAVVKIKESE FEEHGEKWFD QYQLLQTQMK QLELQKEKLL AEKN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgataaag agaactggaa ggtagaagtt gcttctcttg cgaaggagtt gtcttcagac aaaaggaagc ttccttatcc agagatggat gttcaaaata tcagaaagtt gaaaggcctt aaagactcct atgggttaga tggtgtttct gaattgcgtg tagcggaagc agctttattg ggagcaaaaa aacttccgga agagcaaaag caggatactt tgcagagtac gataaaagca ttgagagccg atttgaaggt tcttaacaag aagtttaaaa ggcttccgga atcctatcag cctaaaaaat cgtcagaagc tgtctcagcg aaacgaagca atggaaacgc tgcttcagta gaaaaagagc tagctgctct agaagaaaaa caaaaagagt gccatgctgc ctgcttacaa tttgaaactg aaagttcgag atttttggca gaacggaata gagcaaaatt gtttgagtct aaaatagctc aacaacgtaa agaaagggag gagctaggga atcaggaagc gcgctatact caggtaatca gttgtttaga agcgtttatt gcaagaaaag gcgtatctgc tcagcacaag tctaaagaag atctttctct caaaataact gagttgttgt ctcttaacaa tcagagtctc aaaaaacgag attgtaaccc gctctctagg atgcaaggac gatttcaagc atttgctgaa gaagggtgct tacaagacgt caagggattg ttggaattga atatgtgttt aggttccgcc gaattttctc tgtatcgtga tgaacaaaat cgaatgagtt ccgagagatt tcccttccag aattcgcagc aacttcttca gtacggtaaa aatcttttcg attcttatga taaaggcgat cgaactccac ttttgcagtt tattttagga tctggatggg gggcgattcg tgaagcttgc ttcgagctga aatctttaaa taaacgtaag caaaaaggct cttcttttgc tcctgaagat tatgagaacg cttgtaaagc tttaaagagg ttccttaaag cacgagaaag tctacgtttt aaacttagcc tccctttagg gggaaatagt gagttagaga ttggattgca acatcacata cggaatcagc aatggacaaa ggatcgttta acaggtagac accaagagtg ttgtcgagat attttgcagc atttagaaga ttggattacg aaaactcgaa acgagtccca agaatgccaa aaagtagaaa cggaaataca aaacttttgc caaaaagaag gatcctgcaa aaatttacaa gatagtgtga agattctatt ttctaattta caggcagatc tgaacaaaat tcctcaggat attcttcgag ctgtcttacg ttctctatcc ttgaagcttt gttatattat ggacaagaaa ttagaatgtg agaagttgga ggaaaaattt gctaaggttg acgctgttgt taaaataaaa gagtctgagt tcgaggagca tggagagaag tggtttgatc agtatcaact gttacaaact caaatgaaac agctagagct acaaaaagag aaattattgg cggagaaaaa ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for ChmuA.19921.a.B3.PW38520
PURIFICATION DATe: 8/6/2018
CONCENTRATION: 10mg/ml
OBSERVED MW: 63kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 HEPES, 500mM NaCl, 5% glycerol, 0.025% azide, 2mM DTT
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 90
PERCENT COVERAGE: 98
Protocol Notes
Insoluble protein for antibody production
Validated AA Sequence
MAHHHHHHMD KENWKVEVAS LAKELSSDKR KLPYPEMDVQ NIRKLKGLKD SYGLDGVSEL RVAEAALLGA KKLPEEQKQD TLQSTIKALR ADLKVLNKKF KRLPESYQPK KSSEAVSAKR SNGNAASVEK ELAALEEKQK ECHAACLQFE TESSRFLAER NRAKLFESKI AQQRKEREEL GNQEARYTQV ISCLEAFIAR KGVSAQHKSK EDLSLKITEX XSLNNQSLKK RDCNPLXXXQ GXFQAFAEEG XLQXXXXXXX LXXXXXXXXX XXXXXXXNRX XXXXXXXXXX XXXLQXXXNL FDSYDKGDRT PLLXFXLGSG WGAXREACFE LKSLNKRKQK GSSFAPEDYE NACKALKRFL KARESLRFKL SLPLGGNSEL EIGLQHHIRN QQWTKDRLTG RHQECCRDIL QHLEDWITKT RNESQECQKV ETEIQNFCQK EGSCKNLQDS VKILFSNLQA DLNKIPQDIL RAVLRSLSLK LCYIMDKKLE CEKLEEKFAK VDAVVKIKES EFEEHGEKWF DQYQLLQTQM KQLELQKE
Validated NT Sequence
ttctcttttt gtagctctag ctgtttcatt tgagtttgta acagttgata ctgatcaaac cacttctctc catgctcctc gaactcagac tcttttattt taacaacagc gtcaacctta gcaaattttt cctccaactt ctcacattct aatttcttgt ccataatata acaaagcttc aaggatagag aacgtaagac agctcgaaga atatcctgag gaattttgtt cagatctgcc tgtaaattag aaaatagaat cttcacacta tcttgtaaat ttttgcagga tccttctttt tggcaaaagt tttgtatttc cgtttctact ttttggcatt cttgggactc gtttcgagtt ttcgtaatcc aatcttctaa atgctgcaaa atatctcgac aacactcttg gtgtctacct gttaaacgat cctttgtcca ttgctgattc cgtatgtgat gttgcaatcc aatctctaac tcactatttc cccctaaagg gaggctaagt ttaaaacgta gactttctcg tgctttaagg aacctcttta aagctttaca agcgttctca taatcttcag gagcaaaaga agagcctttt tgcttacgtt tatttaaaga tttcagctcg aagcaagctt cacgantcgc cccccatcca gatcctaaan naaantgcaa aagtggagtt cgatcgcctt tatcataaga atcgaaaaga tttnnnncnt actgaagann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntn cgattttnnt nnnnnngnnn nnnnnnnnat tnnnnnnnnn nnannnnnnn nntcaannnn nncannnnnn nnnnnnnttg taannnccct tcttcagcaa atgcttgaaa nnntccttgc nnnnnnnaga gcgggttaca atctcgtttt ttgagactct gattgttaag agannnnnac tcagttattt tgagagaaag atcttcttta gacttgtgct gagcagatac gccttttctt gcaataaacg cttctaaaca actgattacc tgagtatagc gcgcttcctg attccctagc tcctcccttt ctttacgttg ttgagctatt ttagactcaa acaattttgc tctattccgt tctgccaaaa atctcgaact ttcagtttca aattgtaagc aggcagcatg gcactctttt tgtttttctt ctagagcagc tagctctttt tctactgaag cagcgtttcc attgcttcgt ttcgctgaga cagcttctga cgatttttta ggctgatagg attccggaag ccttttaaac ttcttgttaa gaaccttcaa atcggctctc aatgctttta tcgtactctg caaagtatcc tgcttttgct cttccggaag tttttttgct cccaataaag ctgcttccgc tacacgcaat tcagaaacac catctaaccc ataggagtct ttaaggcctt tcaactttct gatattttga acatccatct ctggataagg aagcttcctt ttgtctgaag acaactcctt cgcaagagaa gcaacttcta ccttccagtt ctctttatcc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHDK ENWKVEVASL AKELSSDKRK LPYPEMDVQN IRKLKGLKDS YGLDGVSELR VAEAALLGAK KLPEEQKQDT LQSTIKALRA DLKVLNKKFK RLPESYQPKK SSEAVSAKRS NGNAASVEKE LAALEEKQKE CHAACLQFET ESSRFLAERN RAKLFESKIA QQRKEREELG NQEARYTQVI SCLEAFIARK GVSAQHKSKE DLSLKITELL SLNNQSLKKR DCNPLSRMQG RFQAFAEEGC LQDVKGLLEL NMCLGSAEFS LYRDEQNRMS SERFPFQNSQ QLLQYGKNLF DSYDKGDRTP LLQFILGSGW GAIREACFEL KSLNKRKQKG SSFAPEDYEN ACKALKRFLK ARESLRFKLS LPLGGNSELE IGLQHHIRNQ QWTKDRLTGR HQECCRDILQ HLEDWITKTR NESQECQKVE TEIQNFCQKE GSCKNLQDSV KILFSNLQAD LNKIPQDILR AVLRSLSLKL CYIMDKKLEC EKLEEKFAKV DAVVKIKESE FEEHGEKWFD QYQLLQTQMK QLELQKEKLL AEKN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGATAAAG AGAACTGGAA GGTAGAAGTT GCTTCTCTTG CGAAGGAGTT GTCTTCAGAC AAAAGGAAGC TTCCTTATCC AGAGATGGAT GTTCAAAATA TCAGAAAGTT GAAAGGCCTT AAAGACTCCT ATGGGTTAGA TGGTGTTTCT GAATTGCGTG TAGCGGAAGC AGCTTTATTG GGAGCAAAAA AACTTCCGGA AGAGCAAAAG CAGGATACTT TGCAGAGTAC GATAAAAGCA TTGAGAGCCG ATTTGAAGGT TCTTAACAAG AAGTTTAAAA GGCTTCCGGA ATCCTATCAG CCTAAAAAAT CGTCAGAAGC TGTCTCAGCG AAACGAAGCA ATGGAAACGC TGCTTCAGTA GAAAAAGAGC TAGCTGCTCT AGAAGAAAAA CAAAAAGAGT GCCATGCTGC CTGCTTACAA TTTGAAACTG AAAGTTCGAG ATTTTTGGCA GAACGGAATA GAGCAAAATT GTTTGAGTCT AAAATAGCTC AACAACGTAA AGAAAGGGAG GAGCTAGGGA ATCAGGAAGC GCGCTATACT CAGGTAATCA GTTGTTTAGA AGCGTTTATT GCAAGAAAAG GCGTATCTGC TCAGCACAAG TCTAAAGAAG ATCTTTCTCT CAAAATAACT GAGTTGTTGT CTCTTAACAA TCAGAGTCTC AAAAAACGAG ATTGTAACCC GCTCTCTAGG ATGCAAGGAC GATTTCAAGC ATTTGCTGAA GAAGGGTGCT TACAAGACGT CAAGGGATTG TTGGAATTGA ATATGTGTTT AGGTTCCGCC GAATTTTCTC TGTATCGTGA TGAACAAAAT CGAATGAGTT CCGAGAGATT TCCCTTCCAG AATTCGCAGC AACTTCTTCA GTACGGTAAA AATCTTTTCG ATTCTTATGA TAAAGGCGAT CGAACTCCAC TTTTGCAGTT TATTTTAGGA TCTGGATGGG GGGCGATTCG TGAAGCTTGC TTCGAGCTGA AATCTTTAAA TAAACGTAAG CAAAAAGGCT CTTCTTTTGC TCCTGAAGAT TATGAGAACG CTTGTAAAGC TTTAAAGAGG TTCCTTAAAG CACGAGAAAG TCTACGTTTT AAACTTAGCC TCCCTTTAGG GGGAAATAGT GAGTTAGAGA TTGGATTGCA ACATCACATA CGGAATCAGC AATGGACAAA GGATCGTTTA ACAGGTAGAC ACCAAGAGTG TTGTCGAGAT ATTTTGCAGC ATTTAGAAGA TTGGATTACG AAAACTCGAA ACGAGTCCCA AGAATGCCAA AAAGTAGAAA CGGAAATACA AAACTTTTGC CAAAAAGAAG GATCCTGCAA AAATTTACAA GATAGTGTGA AGATTCTATT TTCTAATTTA CAGGCAGATC TGAACAAAAT TCCTCAGGAT ATTCTTCGAG CTGTCTTACG TTCTCTATCC TTGAAGCTTT GTTATATTAT GGACAAGAAA TTAGAATGTG AGAAGTTGGA GGAAAAATTT GCTAAGGTTG ACGCTGTTGT TAAAATAAAA GAGTCTGAGT TCGAGGAGCA TGGAGAGAAG TGGTTTGATC AGTATCAACT GTTACAAACT CAAATGAAAC AGCTAGAGCT ACAAAAAGAG AAATTATTGG CGGAGAAAAA CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT