CaalA.01663.b

Cell wall protein IFF2 (Hyphally regulated cell wall protein 3) (HYR3)

CENTER ID: CaalA.01663.b
ORGANISM: Candida albicans
ASSOCIATED DISEASE: Candidiasis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CaalA.01663.b.B21.GE43685 effector domain 17 341
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:C5_00730W_A
UniProt: Q59XA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHLFKRIALT LWLIISSTLA VVITEDRVDR GTISVNLGEV TVNSGASWSI INNAVTAFAG KINVQSGGGL YISSTSPLLA LQVTLTSLLD SITNDGVISL DSRASLTASN YNLIGLSFTN NGEMYLAASG VLPSTMDLTA ASWTNNGLIV AYQNQRSSGV LNLGTPLGAI TNNGQICLYN QVYQQQTRIE GSGCITANKN SAIYISNAVL PVATTQNFYL QDSKSSMIVQ AVSSTQTFNV YGFGNGNKIG ITLPAVGIPP NPAYSYDVDT GILTVRAGLL SQKFNIGLGY YSLFFSVVTD NGAGLPSTIL GSISYSGPVP SRNLPASCKI ACKPVPTAPG TNPTEYTTTI TTTNSAGNPL TETGVVDIST DSNGSWFTTT SIFPTTSQSS SSETVASSSQ PDSSSTEPSA FPSSTGDSSA EPSITSDYSS SELSVVPSSA SESASESSAE PSSASESASE SGSESVASET SASESASEQS STSESVSSEF ASSDSSSEPS SASESSVESS SASEFVVPSS ATETSVSESA SESSAEPSSA SESVASESAV SETSASESAA PSSASETSVS ESAASSSASE SFASESSVES SAVPSSASEF STSESVASET PASETPASET PASESASEQS STSESSAEIS SASESSAEPS SAKSAISESA SEFSAAPSSA SQSSASQSST NESSSQQSSA ESSSTGTSSV SASAATSEYT ATWTTTNSDG SVSTESGIIS QSGSSFTTIT TFVPDATSEY TTTWTTTNSD GSVSTESGIV SQSGSSFTTI TTFAPDATSE YTTTWTTTNS DGSVSTESGV VSQSGTSLTT ITTFAPDVTS EYTTTWTTTN SDGSVSTESG IVSQSGSSFT TITTFEPPVV CKRDDVNCGS SIGLSTPYYG NSSQPLSSTK MTDTSATQTV DSSLSSITDA TTTQSVNSLE TPVPTSGSGN GSNNGSDNGS NNGSNNGSNN GSGSGNGSNN GSNNGSGSGN GFNNGSDNGS NNGSGNASNN GSASGSGSDN GSDNGSDNGS DNGSNNGSNT DNGSNSGSDS GNGIDNGSGN GSSDGSDNGT TNGSGSGGES NNGSGNGSDN GSSPDNGSNN GSNNGSNNGS GDGIGTGSNS DTDNGSGNGS NNGSGSVNGS ANGSGNGSNN GSNSGSNSDN GSNNSSGNGS SSDLGSVSGT GNGSNEGSSN ESGANNGSNN GAGALPAATL SVVPSPSADS GSTSSASAMV IPNTNGSGKL LNGKVLTLSV LSSMVVVFL
NT Sequence
atgcatttgt tcaaaagaat tgctttaacc ttgtggttaa taatcagttc cactttagct gttgttatca ctgaagatag agttgatcgt ggtactatta gtgtgaatct tggcgaagtc actgtcaacc tgggggcttc atggtccatt atcaataatg ctgtaacggc atttgctgga aaaataaacg ttcaatctgg tggtggatta tacatctccc tgacatctcc actcttggcc cttcaagtta ctttaacgtc tttgcttgac ctgatcacaa atgatggggt tatttctttg gattctcgtg cttcgttgac agcttcaaac tataatttga taggtttgtc attcaccaac aatggtgaaa tgtatcttgc tgcttcaggt gtattgccaa gtactatgga tctcactgcc gccagttgga ccaacaatgg gttgattgtt gcttatcaaa atcaaagaag ttccggggtt ttgaatcttg gtaccccatt gggagcaatc accaataatg gtcaaatttg tttatataat caagtttatc aacaacaaac aagaatagag gggtctggtt gtatcactgc taacaaaaac tctgctatct atatttccaa cgcagtgtta ccagttgcta ctactcaaaa cttttattta caagatagta aatcttcaat gattgtacaa gcagtatcca gcactcaaac attcaatgtc tatgggtttg gtaatggtaa taaaattggt ataactcttc cagcagtggg gatccctccc aatcctgcat acagttacga tgttgacaca gggattttga cggtgagagc aggattgctc agtcaaaaat ttaatattgg tcttggatac tattcacttt tttttagtgt tgtcactgat aatggtgctg gcttaccatc aaccatttta ggctcaatat cttattctgg tcctgttcca tcaagaaatt tacccgcatc ttgtaagatt gcttgtaaac ccgtgcctac cgcgccagga acaaatccaa ctgagtacac caccacaata acaacaacaa attctgctgg taacccattg actgaaactg gtgttgttga tattctgacc gattcaaatg gttcttggtt tactactaca tcaattttcc caactacatc tcaatctagt agttctgaaa ctgttgcttc ttctagtcaa ccagattctt catccactga accttctgct ttcccatcat ctactggtga ctcttcagct gaaccatcta taactagtga ctattcatcc agtgaattat ccgttgttcc atcatctgct agtgaatccg ctagtgaatc atcagctgaa ccatcatctg ctagtgagtc agcaagtgaa tctggaagcg aatccgttgc tagtgaaact tcagctagtg aatccgctag tgaacaatct tcaactagtg aatctgtttc aagtgaattt gcttctagtg attcttcttc tgaaccatca tcggccagtg aatcttctgt cgagtcatca tcagccagtg aatttgttgt accatcttca gctactgaaa cctcagttag tgagtctgct agtgaatctt ctgctgaacc atcatctgct agtgaatctg ttgctagtga gtctgctgtt agtgaaacct cagccagtga atcagctgca ccatcatcag ccagtgaaac atcagttagt gagtctgctg cttcatcttc agccagtgaa ctgtttgcca gtgaatcttc tgttgaatca tctgctgttc catcttctgc ttctgaattt tcaactagtg aatctgttgc tagtgaaacc ccagctagtg aaaccccagc tagtgaaacc ccagctagtg aatccgctag tgaacaatct tcaactagtg aatcatctgc tgaaatatcg tctgccagtg aatcttcagc tgaaccatca tctgctaaat cagctattag tgagtctgct agtgaatttt ctgctgctcc atcttcagcc agtcaatctt ctgctagtca atcttcaacc aatgagtcat cttcacaaca atcttccgct gaatcatcat ctactgggac atcttcagtt agtgcttcag cagcaacttc tgaatacact gccacctgga ctaccaccaa ctccgatggg tcagtgtcca ctgaatctgg tatcattagt caatctggtt cttcatttac caccatcacc acatttgttc cagatgctac ttctgaatac actaccacct ggactaccac caactccgat gggtcagtgt ccactgaatc tggtatcgtt agtcaatctg gttcttcatt cactaccatc accacatttg ctccagatgc tacttctgaa tacactacca cctggactac caccaattcc gatggatcag tttccactga atctggtgtt gtcagtcaat ctggcacatc attgacaact atcactacat ttgccccaga tgttacttct gaatacacta ccacctggac taccaccaac tccgatgggt cagtgtccac tgaatctggt atcgttagtc aatctggttc ttcatttacc accattacta cctttgaacc tcctgttgta tgtaagagag atgatgtgaa ttgtggttct tcaattggtt tatctacacc ttattatgga aattcttctc aaccattatc atcaacaaaa atgacagata cctcagctac ccaaactgtg gattcatcat tgtcttcaat tactgatgcc acaactacac agtcagtaaa tagtcttgaa acacctgtcc caactagtgg atctggcaat ggatccaata atggctcgga taatggctcc aataatggct ccaataatgg ttcaaataat ggctctggat ctggtaatgg atccaataat ggttcaaata atggctctgg atctggtaat ggattcaata atggctcgga taatggctcc aataatggat ctggtaatgc ttctaacaat ggctctgcca gtgggtcagg ttctgataat ggttctgata atggttctga taatggttct gataatggtt ccaacaatgg ctccaatact gacaatggat ctaatagtgg gtcagattct ggtaatggaa tagataatgg ctcgggtaac ggttctagtg atggttccga taatggaact accaatggct cgggttccgg tggggaatct aacaacggct caggtaatgg atcagataac ggctcgagtc ctgacaatgg ttctaataat ggttctaata atggttctaa taatggttca ggtgatggaa taggtactgg atcgaacagt gacaccgata atggttctgg taatggatct aataatggct caggctctgt taacggatct gctaatgggt ctggtaatgg ttctaacaat ggttcaaata gtggttctaa ttccgacaat ggctctaaca acagttctgg taatggatcc agtagtgact tgggctctgt tagtggaaca ggcaatggat ccaacgaagg ctcaagtaat gaatcaggtg ccaacaatgg atctaacaat ggtgctggtg ctcttcctgc cgctacactc tcggttgttc catcaccaag tgctgactcc ggctcaacta gctccgcgtc ggctatggtt attccaaata ccaatggctc tggtaaatta cttaatggaa aagtacttac cttgagtgtg ttgtcttcaa tggttgttgt tttcttgtaa
Details for CaalA.01663.b.B21.GE43685
HARVESTED ON: data unavailable
SEQUENCED ON: 6/23/2020
EXPECTED MW: 35kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 65
Validated AA Sequence
MYLAASGVLP STMDLTAASW TNNGLIVAYQ NQRSSGVLNL GTPLGAITNN GQICLYNQVY QQQTRIEGSG CITANKNSAI YISNAVLPVA TTQNFYLQDS KSSMIVQAVS STQTFNVYGF GNGNKIGITL PAVGIPPNPA YSYDVDTGIL TVRAGLLSQK FNIGLGYYSL FFSVVTDNGA GLPSTILGSI SYSGPVPSRN LPASCKIACK PXPTAPGT
Validated NT Sequence
ttnnngggnt nngttagcag ccggatccta tcttactcac ttatgtacct ggagcggtgg ggnncggttt gcaggcgatt ttacacgacg caggtaagtt gcgactggga acaggcccgc tgtacgaaat gcttcccaga atcgtggagg gtaatcctgc tccgttatcg gtcacaactg aaaagaacaa actgtaatat cccaacccga tgttgaactt ttgactaagc aaaccggcac gcacagttaa gatcccggta tctacgtcat aagagtaggc aggattaggc gggataccta ccgcaggcag cgtgatgccg attttgttac cattcccgaa cccgtacacg ttgaacgtct gtgtagagga taccgcttgc acaatcatgg acgatttcga atcttgaagg taaaagttct gcgtcgttgc tacaggaaga acggcattac taatataaat agcgctattt ttgttagcgg taatacaacc gctgccttcg atgcgggtct gttgctggta aacttggttg tacaggcaga tttgaccgtt gttggtaata gcgcctaagg gcgtcccaag gttcaaaaca cccgagctgc gttggttttg gtatgcaacg ataagcccgt tattagtcca agaggcagcg gtcaaatcca ttgtagaagg aagcacacca gatgcggcaa ggtacatctc cccattgttc gtaaaagata atccgataag attgtaattc gatgcggtca gcgacgcacg ggaatccaac gaaatcacac cgtcgttcgt gatggagtcc agcagcgacg tcagcgttac ctgcagagcc aacaagggcg atgttgagga gatgtacaag ccgcccccgg attgaacatt gatctttcct gcaaacgcag ttaccgcatt gttgataata ctccangacg ctccggagtt tactgtcacc tcgcctaagt tcacggaaat ggtaccacga tcaacacgat cttcggtaat cactacagcc aatgtggnnn natggtggng gng
Expected Protein Sequence
MAHHHHHHST LAVVITEDRV DRGTISVNLG EVTVNSGASW SIINNAVTAF AGKINVQSGG GLYISSTSPL LALQVTLTSL LDSITNDGVI SLDSRASLTA SNYNLIGLSF TNNGEMYLAA SGVLPSTMDL TAASWTNNGL IVAYQNQRSS GVLNLGTPLG AITNNGQICL YNQVYQQQTR IEGSGCITAN KNSAIYISNA VLPVATTQNF YLQDSKSSMI VQAVSSTQTF NVYGFGNGNK IGITLPAVGI PPNPAYSYDV DTGILTVRAG LLSQKFNIGL GYYSLFFSVV TDNGAGLPST ILGSISYSGP VPSRNLPASC KIACKPVPTA PGT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccacat tggctgtagt gattaccgaa gatcgtgttg atcgtggtac catttccgtg aacttaggcg aggtgacagt aaactccgga gcgtcctgga gtattatcaa caatgcggta actgcgtttg caggaaagat caatgttcaa tccgggggcg gcttgtacat ctcctcaaca tcgcccttgt tggctctgca ggtaacgctg acgtcgctgc tggactccat cacgaacgac ggtgtgattt cgttggattc ccgtgcgtcg ctgaccgcat cgaattacaa tcttatcgga ttatctttta cgaacaatgg ggagatgtac cttgccgcat ctggtgtgct tccttctaca atggatttga ccgctgcctc ttggactaat aacgggctta tcgttgcata ccaaaaccaa cgcagctcgg gtgttttgaa ccttgggacg cccttaggcg ctattaccaa caacggtcaa atctgcctgt acaaccaagt ttaccagcaa cagacccgca tcgaaggcag cggttgtatt accgctaaca aaaatagcgc tatttatatt agtaatgccg ttcttcctgt agcaacgacg cagaactttt accttcaaga ttcgaaatcg tccatgattg tgcaagcggt atcctctaca cagacgttca acgtgtacgg gttcgggaat ggtaacaaaa tcggcatcac gctgcctgcg gtaggtatcc cgcctaatcc tgcctactct tatgacgtag ataccgggat cttaactgtg cgtgccggtt tgcttagtca aaagttcaac atcgggttgg gatattacag tttgttcttt tcagttgtga ccgataacgg agcaggatta ccctccacga ttctgggaag catttcgtac agcgggcctg ttcccagtcg caacttacct gcgtcgtgta aaatcgcctg caaaccggtc cccaccgctc caggtacatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat