CaalA.00629.a
Acetyl-coenzyme A synthetase 2 (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acyl-activating enzyme 2)
| CENTER ID: | CaalA.00629.a |
| ORGANISM: | Candida albicans |
| ASSOCIATED DISEASE: | Candidiasis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CaalA.00629.a.FS11.GE00455 | full length | 2 | 672 | ||
| CaalA.00629.a.HA11.GE00475 | full length | 36 | 667 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CaalA.00629.a.FS11.PD00399 | full length | 2 | 672 | ||
| CaalA.00629.a.HA11.PD00408 | full length | 36 | 667 |
Structures
7KDS
DEPOSITED: 10/9/2020
DETERMINATION: XRay
CLONE: CaalA.00629.a.FS11.GE00455
PROTEIN: CaalA.00629.a.FS11.PD00399
8V4O
DEPOSITED: 11/29/2023
DETERMINATION: XRay
CLONE: CaalA.00629.a.FS11.GE00455
PROTEIN: CaalA.00629.a.FS11.PD00399
8V4P
DEPOSITED: 11/29/2023
DETERMINATION: XRay
CLONE: CaalA.00629.a.FS11.GE00455
PROTEIN: CaalA.00629.a.FS11.PD00399
8V4R
DEPOSITED: 11/29/2023
DETERMINATION: XRay
CLONE: CaalA.00629.a.FS11.GE00455
PROTEIN: CaalA.00629.a.FS11.PD00399
8W0B
DEPOSITED: 2/13/2024
DETERMINATION: XRay
CLONE: CaalA.00629.a.FS11.GE00455
PROTEIN: CaalA.00629.a.FS11.PD00399
8W0C
DEPOSITED: 2/13/2024
DETERMINATION: XRay
CLONE: CaalA.00629.a.FS11.GE00455
PROTEIN: CaalA.00629.a.FS11.PD00399
8W0D
DEPOSITED: 2/13/2024
DETERMINATION: XRay
CLONE: CaalA.00629.a.FS11.GE00455
PROTEIN: CaalA.00629.a.FS11.PD00399
8W0H
DEPOSITED: 2/13/2024
DETERMINATION: XRay
CLONE: CaalA.00629.a.FS11.GE00455
PROTEIN: CaalA.00629.a.FS11.PD00399
8W0J
DEPOSITED: 2/13/2024
DETERMINATION: XRay
CLONE: CaalA.00629.a.FS11.GE00455
PROTEIN: CaalA.00629.a.FS11.PD00399
8W0L
DEPOSITED: 2/13/2024
DETERMINATION: XRay
CLONE: CaalA.00629.a.FS11.GE00455
PROTEIN: CaalA.00629.a.FS11.PD00399
8W0M
DEPOSITED: 2/13/2024
DETERMINATION: XRay
CLONE: CaalA.00629.a.FS11.GE00455
PROTEIN: CaalA.00629.a.FS11.PD00399
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:orf19.1064 |
| RefSeq: | XP_713713.1 |
| UniProt: | Q8NJN3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPTEQTHNVV HEANGVKLRE TPKEFFERQP NKGHIHDVNQ YKQMYEQSIK DPQGFFGPLA KELLSWDHDF HTVKSGTLKN GDAAWFLGGE LNASYNCVDR HAFANPDKPA LICEADDEKD SHILTYGDLL REVSKVAGVL QSWGIKKGDT VAVYLPMNAQ AIIAMLAIAR LGAAHSVIFA GFSAGSIKDR VNDASCKALI TCDEGKRGGR TTNIKKLCDE ALVDCPTVEK VLVYKRTNNP EIHLTEGRDY YWDVETAKFP GYLPPVSVNS EDPLFLLYTS GSTGTPKGVV HSTAGYLLGA ALSTKYIFDI HPEDILFTAG DVGWITGHTY ALYGPLLLGV PTIIFEGTPA YPDYGRFWQI VEKHKATHFY VAPTALRLLR KAGEQEIAKY DLSSLRTLGS VGEPISPDIW EWYNEFVGKN QCHISDTYWQ TESGSHLIAP LAGVVPNKPG SASYPFFGID AALIDPVTGV EIEGNDAEGV LAIKDHWPSM ARTVYKNHTK YMDTYMNPYP GYYFTGDGAA RDHDGYYWIR GRVDDVVNVS GHRLSTAEIE AALIEDKKVS EAAVVGIHDD ITGQAVIAYV ALKEGNSDED SEGLRKELVL QVRKTIGPFA APKSVIIVQD LPKTRSGKIM RRILRKVSSN EADQLGDIST LSNPQSVEGI ISAFGAQFGK K
NT Sequence
atgccaacag aacaaactca taatgtcgtc catgaagcca atggcgtcaa attaagagaa acccctaaag aattttttga aagacaacca aataaaggtc acattcatga tgtcaatcaa tacaaacaaa tgtatgaaca atctattaaa gatcctcaag gatttttcgg tccattggcc aaagaattgt tatcttggga tcacgatttc cacactgtca aatcaggtac tttgaaaaat ggtgatgcag cttggttttt gggtggtgaa ttgaatgctt cttacaactg tgttgataga catgcatttg ctaacccaga taaaccagct cttatttgtg aagctgatga cgaaaaagat tcccatatat tgacttacgg tgatttgtta agagaagttt ctaaagtagc tggtgtttta caatcttggg ggatcaagaa aggtgacact gttgctgttt acttaccaat gaatgcccaa gctatcattg ctatgttggc tattgctaga ttgggtgctg cccattcagt tatttttgct gggttctcgg ctggttccat taaagacaga gtcaacgatg ctagttgtaa agctcttatc acttgtgatg aaggtaaaag aggtggtaga accactaaca ttaaaaaatt atgtgatgag gctttggttg attgtcctac tgttgaaaaa gttttggttt ataaaagaac taataatcca gaaattcatt taactgaagg tagagattac tattgggatg ttgaaactgc caaattccca ggatacttgc caccagtttc agtcaactct gaagatccat tgttcttatt gtatacttct ggttctactg gtactccaaa gggggttgtt cattctactg ctggttactt attgggtgct gctctttcca ctaaatacat ttttgatatt catccagaag atatcttgtt tactgctggt gatgttggtt ggattactgg tcacacctat gccttgtatg gtccattgtt attaggtgtc ccaaccatta ttttcgaagg taccccggct tatccagatt acggaagatt ctggcaaatt gttgaaaaac acaaggctac tcatttctat gttgctccaa ctgctttaag attattacgt aaagctggtg aacaagaaat tgctaaatac gatttgagtt ctttgagaac tttaggttct gttggtgaac caatttctcc agacatttgg gaatggtata atgaatttgt tggtaaaaac caatgtcaca tttctgacac ttattggcaa actgaatctg gatctcattt aattgcccca ttggctggtg ttgttccaaa caagccaggt tctgcttctt atccattctt tggtattgat gctgccttga ttgatccagt cactggtgtt gaaattgaag gaaatgatgc tgaaggtgtt ttggctatca aggaccattg gccatcaatg gcaagaactg tctacaagaa ccacaccaaa tatatggata cctacatgaa tccataccca ggttattact ttaccggtga tggtgctgct agagatcatg atggatacta ctggattaga ggtagagttg atgatgttgt caatgtcagt ggtcatagat tatccactgc tgaaattgaa gctgctttaa ttgaagacaa aaaagtcagt gaagctgctg ttgttggtat tcatgacgac attactggtc aagctgtcat tgcttacgtt gccttgaaag aaggaaacag tgacgaggat tctgaaggat taagaaaaga attggtttta caagttagaa aaactattgg tccatttgct gcaccaaaat ctgttataat tgtccaagat ttgccaaaga ctagatctgg taaaattatg agaagaattt tgagaaaagt cagttctaat gaagctgatc aattgggtga tatttctact ttatctaacc cacaatcagt tgaaggtata atttctgcct ttggtgccca attcggtaag aaataa
Details for CaalA.00629.a.FS11.GE00455
| HARVESTED ON: | 8/7/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 76kDa |
| OBSERVED MW: | 76kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | Data not available |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MHHHHHHHHE NLYFQGPTEQ THNVVHEANG VKLRETPKEF FERQPNKGHI HDVNQYKQMY EQSIKDPQGF FGPLAKELLS WDHDFHTVKS GTLKNGDAAW FLGGELNASY NCVDRHAFAN PDKPALICEA DDEKDSHILT YGDLLREVSK VAGVLQSWGI KKGDTVAVYL PMNAQAIIAM LAIARLGAAH SVIFAGFSAG SIKDRVNDAS CKALITCDEG KRGGRTTNIK KLCDEALVDC PTVEKVLVYK RTNNPEIHLT EGRDYYWDVE TAKFPGYLPP VSVNSEDPLF LLYTSGSTGT PKGVVHSTAG YLLGAALSTK YIFDIHPEDI LFTAGDVGWI TGHTYALYGP LLLGVPTIIF EGTPAYPDYG RFWQIVEKHK ATHFYVAPTA LRLLRKAGEQ EIAKYDLSSL RTLGSVGEPI SPDIWEWYNE FVGKNQCHIS DTYWQTESGS HLIAPLAGVV PNKPGSASYP FFGIDAALID PVTGVEIEGN DAEGVLAIKD HWPSMARTVY KNHTKYMDTY MNPYPGYYFT GDGAARDHDG YYWIRGRVDD VVNVSGHRLS TAEIEAALIE DKKVSEAAVV GIHDDITGQA VIAYVALKEG NSDEDSEGLR KELVLQVRKT IGPFAAPKSV IIVQDLPKTR SGKIMRRILR KVSSNEADQL GDISTLSNPQ SVEGIISAFG AQFGKK
Full NT Sequence (Expression Vector + Insert)
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aacttttaga gaccaaggag gtaaaaaatg caccatcacc atcatcacca ccacgagaat ttgtatttcc agggtcctac ggaacaaacc cataatgttg ttcacgaagc aaacggtgtt aagctgcgcg aaaccccgaa agaatttttc gaacgccagc cgaacaaagg tcatatccat gacgttaacc agtacaaaca aatgtatgag cagagcatca aagatccgca gggtttcttc ggcccattgg cgaaagaact gctgagctgg gatcatgatt ttcatacggt gaagtctggt actctgaaga atggtgacgc ggcatggttc ctgggtggcg agctgaatgc cagctacaac tgcgtagatc gtcacgcgtt cgcgaacccg gacaaaccgg cactgatttg cgaggccgac gatgagaaag acagccacat cctgacctac ggcgatttgc tgcgtgaagt gagcaaagtc gccggtgtgc tgcagtcttg gggtatcaag aaaggcgaca cggtcgcggt gtatctgccg atgaacgcgc aagcgatcat tgcgatgctg gccattgcac gtctgggtgc ggcgcactcc gtgatttttg cgggtttttc cgctggcagc attaaagatc gcgttaatga cgctagctgt aaggcactga ttacctgtga tgagggcaag cgtggtggcc gcaccaccaa catcaagaaa ctgtgcgacg aagcgctggt cgactgcccg acggtcgaaa aggtcctggt gtacaagcgc accaacaatc cagagattca tctgaccgag ggtcgtgatt actattggga tgtcgaaacc gcgaagttcc cgggttactt gccgcctgtt tcagtcaaca gcgaagatcc gctgtttctg ctgtacacca gcggtagcac cggtacgcca aaaggcgtgg ttcatagcac tgcaggctat ctgctgggtg cagcactgag cactaagtac atcttcgata tccatccgga agatatcctg ttcaccgccg gtgacgtggg ctggatcacc ggtcatactt acgcattata cggtccgctc ctgctgggcg ttccgaccat tatcttcgag ggtacgccgg catacccgga ctatggtcgt ttctggcaaa ttgttgagaa gcacaaggcc acccactttt atgttgcccc gaccgcgctg cgtctgctgc gtaaggctgg cgagcaagaa attgcgaaat atgaccttag cagcctgcgt acgctgggct cggttggcga gccgattagc ccggatattt gggagtggta taatgaattt gttggcaaga accaatgtca catcagcgat acctactggc agaccgaatc cggcagccac ttgatcgcgc cactggcggg tgtggtcccg aacaaaccgg gcagcgcatc gtatccgttc tttggtattg acgccgcatt gatcgaccct gtgaccggtg ttgagattga gggtaatgac gctgagggcg ttctggcgat caaagaccac tggccgagca tggcgcgtac ggtctataag aaccacacca aatacatgga cacgtatatg aatccgtacc cgggttatta ctttacgggt gacggtgctg cgcgcgacca cgatggctac tactggattc gtggccgtgt tgacgatgtc gtgaacgtca gcggtcaccg tctgagcacg gccgagatcg aagcggcgct gattgaagat aagaaagtct ccgaggcagc tgttgtgggt attcacgacg acattacggg ccaagctgtt attgcgtacg tggcattgaa agagggcaat agcgatgagg actctgaggg cctgcgcaaa gaactggtgc tgcaagtgcg caagaccatc ggtccgttcg cagcgccgaa aagcgtgatc attgtacaag acttgccgaa aacgcgcagc ggtaagatca tgcgtcgtat cctccgtaag gtcagctcca atgaagcgga ccagctgggt gatattagca cgctgagcaa tccgcagtct gttgagggta tcatcagcgc ctttggtgca cagtttggca aaaagtgata aggttgaggt ctcaccccct agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgcccc tgagacgcgt caatcgagtt cgtacctaag ggcgacaccc cctaattagc ccgggcgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg catggggagt ccccacacta ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaaca aggggtgtta tgagccatat tcaggtataa atgggctcgc gataatgttc agaattggtt aattggttgt aacactgacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagaatatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcca cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcgtt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcactcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgcctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaagcgg cgcgccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatat gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgag gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag atagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgccagt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg a
Details for CaalA.00629.a.HA11.GE00475
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 71kDa |
| OBSERVED MW: | 71kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MHHHHHHHHE NLYFQGHDVN QYKQMYEQSI KDPQGFFGPL AKELLSWDHD FHTVKSGTLK NGDAAWFLGG ELNASYNCVD RHAFANPDKP ALICEADDEK DSHILTYGDL LREVSKVAGV LQSWGIKKGD TVAVYLPMNA QAIIAMLAIA RLGAAHSVIF AGFSAGSIKD RVNDASCKAL ITCDEGKRGG RTTNIKKLCD EALVDCPTVE KVLVYKRTNN PEIHLTEGRD YYWDVETAKF PGYLPPVSVN SEDPLFLLYT SGSTGTPKGV VHSTAGYLLG AALSTKYIFD IHPEDILFTA GDVGWITGHT YALYGPLLLG VPTIIFEGTP AYPDYGRFWQ IVEKHKATHF YVAPTALRLL RKAGEQEIAK YDLSSLRTLG SVGEPISPDI WEWYNEFVGK NQCHISDTYW QTESGSHLIA PLAGVVPNKP GSASYPFFGI DAALIDPVTG VEIEGNDAEG VLAIKDHWPS MARTVYKNHT KYMDTYMNPY PGYYFTGDGA ARDHDGYYWI RGRVDDVVNV SGHRLSTAEI EAALIEDKKV SEAAVVGIHD DITGQAVIAY VALKEGNSDE DSEGLRKELV LQVRKTIGPF AAPKSVIIVQ DLPKTRSGKI MRRILRKVSS NEADQLGDIS TLSNPQSVEG IISAFGA
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgcaccatc accaccacca tcatcacgag aatctgtact tccaaggcca tgacgttaac cagtacaaac aaatgtatga gcagagcatc aaagatccgc agggtttctt cggcccattg gcgaaagaac tgctgagctg ggatcatgat tttcatacgg tgaagtctgg tactctgaag aatggtgacg cggcatggtt cctgggtggc gagctgaatg ccagctacaa ctgcgtagat cgtcacgcgt tcgcgaaccc ggacaaaccg gcactgattt gcgaggccga cgatgagaaa gacagccaca tcctgaccta cggcgatttg ctgcgtgaag tgagcaaagt cgccggtgtg ctgcagtctt ggggtatcaa gaaaggcgac acggtcgcgg tgtatctgcc gatgaacgcg caagcgatca ttgcgatgct ggccattgca cgtctgggtg cggcgcactc cgtgattttt gcgggttttt ccgctggcag cattaaagat cgcgttaatg acgctagctg taaggcactg attacctgtg atgagggcaa gcgtggtggc cgcaccacca acatcaagaa actgtgcgac gaagcgctgg tcgactgccc gacggtcgaa aaggtcctgg tgtacaagcg caccaacaat ccagagattc atctgaccga gggtcgtgat tactattggg atgtcgaaac cgcgaagttc ccgggttact tgccgcctgt ttcagtcaac agcgaagatc cgctgtttct gctgtacacc agcggtagca ccggtacgcc aaaaggcgtg gttcatagca ctgcaggcta tctgctgggt gcagcactga gcactaagta catcttcgat atccatccgg aagatatcct gttcaccgcc ggtgacgtgg gctggatcac cggtcatact tacgcattat acggtccgct cctgctgggc gttccgacca ttatcttcga gggtacgccg gcatacccgg actatggtcg tttctggcaa attgttgaga agcacaaggc cacccacttt tatgttgccc cgaccgcgct gcgtctgctg cgtaaggctg gcgagcaaga aattgcgaaa tatgacctta gcagcctgcg tacgctgggc tcggttggcg agccgattag cccggatatt tgggagtggt ataatgaatt tgttggcaag aaccaatgtc acatcagcga tacctactgg cagaccgaat ccggcagcca cttgatcgcg ccactggcgg gtgtggtccc gaacaaaccg ggcagcgcat cgtatccgtt ctttggtatt gacgccgcat tgatcgaccc tgtgaccggt gttgagattg agggtaatga cgctgagggc gttctggcga tcaaagacca ctggccgagc atggcgcgta cggtctataa gaaccacacc aaatacatgg acacgtatat gaatccgtac ccgggttatt actttacggg tgacggtgct gcgcgcgacc acgatggcta ctactggatt cgtggccgtg ttgacgatgt cgtgaacgtc agcggtcacc gtctgagcac ggccgagatc gaagcggcgc tgattgaaga taagaaagtc tccgaggcag ctgttgtggg tattcacgac gacattacgg gccaagctgt tattgcgtac gtggcattga aagagggcaa tagcgatgag gactctgagg gcctgcgcaa agaactggtg ctgcaagtgc gcaagaccat cggtccgttc gcagcgccga aaagcgtgat cattgtacaa gacttgccga aaacgcgcag cggtaagatc atgcgtcgta tcctccgtaa ggtcagctcc aatgaagcgg accagctggg tgatattagc acgctgagca atccgcagtc tgttgagggt atcatcagcg cctttggtgc atgataagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gat
Details for CaalA.00629.a.FS11.PD00399
| PURIFICATION DATe: | 9/30/2015 |
| CONCENTRATION: | 20.15mg/ml |
| OBSERVED MW: | 75kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM Tris, pH = 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol |
| EXPRESSION HOST: | Data not available |
| VIAL COUNT (approx.): | 109 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MHHHHHHHHE NLYFQGPTEQ THNVVHEANG VKLRETPKEF FERQPNKGHI HDVNQYKQMY EQSIKDPQGF FGPLAKELLS WDHDFHTVKS GTLKNGDAAW FLGGELNASY NCVDRHAFAN PDKPALICEA DDEKDSHILT YGDLLREVSK VAGVLQSWGI KKGDTVAVYL PMNAQAIIAM LAIARLGAAH SVIFAGFSAG SIKDRVNDAS CKALITCDEG KRGGRTTNIK KLCDEALVDC PTVEKVLVYK RTNNPEIHLT EGRDYYWDVE TAKFPGYLPP VSVNSEDPLF LLYTSGSTGT PKGVVHSTAG YLLGAALSTK YIFDIHPEDI LFTAGDVGWI TGHTYALYGP LLLGVPTIIF EGTPAYPDYG RFWQIVEKHK ATHFYVAPTA LRLLRKAGEQ EIAKYDLSSL RTLGSVGEPI SPDIWEWYNE FVGKNQCHIS DTYWQTESGS HLIAPLAGVV PNKPGSASYP FFGIDAALID PVTGVEIEGN DAEGVLAIKD HWPSMARTVY KNHTKYMDTY MNPYPGYYFT GDGAARDHDG YYWIRGRVDD VVNVSGHRLS TAEIEAALIE DKKVSEAAVV GIHDDITGQA VIAYVALKEG NSDEDSEGLR KELVLQVRKT IGPFAAPKSV IIVQDLPKTR SGKIMRRILR KVSSNEADQL GDISTLSNPQ SVEGIISAFG AQFGKK
Full NT Sequence (Expression Vector + Insert)
TTAATAAGAT GATCTTCTTG AGATCGTTTT GGTCTGCGCG TAATCTCTTG CTCTGAAAAC GAAAAAACCG CCTTGCAGGG CGGTTTTTCG AAGGTTCTCT GAGCTACCAA CTCTTTGAAC CGAGGTAACT GGCTTGGAGG AGCGCAGTCA CCAAAACTTG TCCTTTCAGT TTAGCCTTAA CCGGCGCATG ACTTCAAGAC TAACTCCTCT AAATCAATTA CCAGTGGCTG CTGCCAGTGG TGCTTTTGCA TGTCTTTCCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGACTGA ACGGGGGGTT CGTGCATACA GTCCAGCTTG GAGCGAACTG CCTACCCGGA ACTGAGTGTC AGGCGTGGAA TGAGACAAAC GCGGCCATAA CAGCGGAATG ACACCGGTAA ACCGAAAGGC AGGAACAGGA GAGCGCACGA GGGAGCCGCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCACT GATTTGAGCG TCAGATTTCG TGATGCTTGT CAGGGGGGCG GAGCCTATGG AAAAACGGCT TTGCCGCGGC CCTCTCACTT CCCTGTTAAG TATCTTCCTG GCATCTTCCA GGAAATCTCC GCCCCGTTCG TAAGCCATTT CCGCTCGCCG CAGTCGAACG ACCGAGCGTA GCGAGTCAGT GAGCGAGGAA GCGGAATATA TCCTGTATCA CATATTCTGC TGACGCACCG GTGCAGCCTT TTTTCTCCTG CCACATGAAG CACTTCACTG ACACCCTCAT CAGTGCCAAC ATAGTAAGCC AGTATACACT CCGCTAGCGC TGAGGTCCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGGCG AGAGTAGGGA ACTGCCAGGC ATCAAACTAA GCAGAAGGCC CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTCT GTGTTGTAAA ACGACGGCCA GTCTTAAGCT CGGGCCCCCT GGGCGGTTCT GATAACGAGT AATCGTTAAT CCGCAAATAA CGTAAAAACC CGCTTCGGCG GGTTTTTTTA TGGGGGGAGT TTAGGGAAAG AGCATTTGTC AGAATATTTA AGGGCGCCTG TCACTTTGCT TGATATATGA GAATTATTTA ACCTTATAAA TGAGAAAAAA GCAACGCACT TTAAATAAGA TACGTTGCTT TTTCGATTGA TGAACACCTA TAATTAAACT ATTCATCTAT TATTTATGAT TTTTTGTATA TACAATATTT CTAGTTTGTT AAAGAGAATT AAGAAAATAA ATCTCGAAAA TAATAAAGGG AAAATCAGTT TTTGATATCA AAATTATACA TGTCAACGAT AATACAAAAT ATAATACAAA CTATAAGATG TTATCAGTAT TTATTATGCA TTTAGAATAA ATTTTGTGTC GCCCTTCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC CTCTAGAAAT AATTTTGTTT AACTTTTAGA GACCAAGGAG GTAAAAAATG CACCATCACC ATCATCACCA CCACGAGAAT TTGTATTTCC AGGGTCCTAC GGAACAAACC CATAATGTTG TTCACGAAGC AAACGGTGTT AAGCTGCGCG AAACCCCGAA AGAATTTTTC GAACGCCAGC CGAACAAAGG TCATATCCAT GACGTTAACC AGTACAAACA AATGTATGAG CAGAGCATCA AAGATCCGCA GGGTTTCTTC GGCCCATTGG CGAAAGAACT GCTGAGCTGG GATCATGATT TTCATACGGT GAAGTCTGGT ACTCTGAAGA ATGGTGACGC GGCATGGTTC CTGGGTGGCG AGCTGAATGC CAGCTACAAC TGCGTAGATC GTCACGCGTT CGCGAACCCG GACAAACCGG CACTGATTTG CGAGGCCGAC GATGAGAAAG ACAGCCACAT CCTGACCTAC GGCGATTTGC TGCGTGAAGT GAGCAAAGTC GCCGGTGTGC TGCAGTCTTG GGGTATCAAG AAAGGCGACA CGGTCGCGGT GTATCTGCCG ATGAACGCGC AAGCGATCAT TGCGATGCTG GCCATTGCAC GTCTGGGTGC GGCGCACTCC GTGATTTTTG CGGGTTTTTC CGCTGGCAGC ATTAAAGATC GCGTTAATGA CGCTAGCTGT AAGGCACTGA TTACCTGTGA TGAGGGCAAG CGTGGTGGCC GCACCACCAA CATCAAGAAA CTGTGCGACG AAGCGCTGGT CGACTGCCCG ACGGTCGAAA AGGTCCTGGT GTACAAGCGC ACCAACAATC CAGAGATTCA TCTGACCGAG GGTCGTGATT ACTATTGGGA TGTCGAAACC GCGAAGTTCC CGGGTTACTT GCCGCCTGTT TCAGTCAACA GCGAAGATCC GCTGTTTCTG CTGTACACCA GCGGTAGCAC CGGTACGCCA AAAGGCGTGG TTCATAGCAC TGCAGGCTAT CTGCTGGGTG CAGCACTGAG CACTAAGTAC ATCTTCGATA TCCATCCGGA AGATATCCTG TTCACCGCCG GTGACGTGGG CTGGATCACC GGTCATACTT ACGCATTATA CGGTCCGCTC CTGCTGGGCG TTCCGACCAT TATCTTCGAG GGTACGCCGG CATACCCGGA CTATGGTCGT TTCTGGCAAA TTGTTGAGAA GCACAAGGCC ACCCACTTTT ATGTTGCCCC GACCGCGCTG CGTCTGCTGC GTAAGGCTGG CGAGCAAGAA ATTGCGAAAT ATGACCTTAG CAGCCTGCGT ACGCTGGGCT CGGTTGGCGA GCCGATTAGC CCGGATATTT GGGAGTGGTA TAATGAATTT GTTGGCAAGA ACCAATGTCA CATCAGCGAT ACCTACTGGC AGACCGAATC CGGCAGCCAC TTGATCGCGC CACTGGCGGG TGTGGTCCCG AACAAACCGG GCAGCGCATC GTATCCGTTC TTTGGTATTG ACGCCGCATT GATCGACCCT GTGACCGGTG TTGAGATTGA GGGTAATGAC GCTGAGGGCG TTCTGGCGAT CAAAGACCAC TGGCCGAGCA TGGCGCGTAC GGTCTATAAG AACCACACCA AATACATGGA CACGTATATG AATCCGTACC CGGGTTATTA CTTTACGGGT GACGGTGCTG CGCGCGACCA CGATGGCTAC TACTGGATTC GTGGCCGTGT TGACGATGTC GTGAACGTCA GCGGTCACCG TCTGAGCACG GCCGAGATCG AAGCGGCGCT GATTGAAGAT AAGAAAGTCT CCGAGGCAGC TGTTGTGGGT ATTCACGACG ACATTACGGG CCAAGCTGTT ATTGCGTACG TGGCATTGAA AGAGGGCAAT AGCGATGAGG ACTCTGAGGG CCTGCGCAAA GAACTGGTGC TGCAAGTGCG CAAGACCATC GGTCCGTTCG CAGCGCCGAA AAGCGTGATC ATTGTACAAG ACTTGCCGAA AACGCGCAGC GGTAAGATCA TGCGTCGTAT CCTCCGTAAG GTCAGCTCCA ATGAAGCGGA CCAGCTGGGT GATATTAGCA CGCTGAGCAA TCCGCAGTCT GTTGAGGGTA TCATCAGCGC CTTTGGTGCA CAGTTTGGCA AAAAGTGATA AGGTTGAGGT CTCACCCCCT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCCCC TGAGACGCGT CAATCGAGTT CGTACCTAAG GGCGACACCC CCTAATTAGC CCGGGCGAAA GGCCCAGTCT TTCGACTGAG CCTTTCGTTT TATTTGATGC CTGGCAGTTC CCTACTCTCG CATGGGGAGT CCCCACACTA CCATCGGCGC TACGGCGTTT CACTTCTGAG TTCGGCATGG GGTCAGGTGG GACCACCGCG CTACTGCCGC CAGGCAAACA AGGGGTGTTA TGAGCCATAT TCAGGTATAA ATGGGCTCGC GATAATGTTC AGAATTGGTT AATTGGTTGT AACACTGACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAATATGA GCCATATTCA ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCA CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCGTT CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCACTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGCCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAAGCGG CGCGCCATCG AATGGCGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC GGAAGAGAGT CAATTCAGGG TGGTGAATAT GAAACCAGTA ACGTTATACG ATGTCGCAGA GTATGCCGGT GTCTCTTATC AGACCGTTTC CCGCGTGGTG AACCAGGCCA GCCACGTTTC TGCGAAAACG CGGGAAAAAG TGGAAGCGGC GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA CAACTGGCGG GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG TGCCAGCGTG GTGGTGTCGA TGGTAGAACG AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT GCACAATCTT CTCGCGCAAC GCGTCAGTGG GCTGATCATT AACTATCCGC TGGATGACCA GGATGCCATT GCTGTGGAAG CTGCCTGCAC TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA CCCATCAACA GTATTATTTT CTCCCATGAG GACGGTACGC GACTGGGCGT GGAGCATCTG GTCGCATTGG GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG GCATAAATAT CTCACTCGCA ATCAAATTCA GCCGATAGCG GAACGGGAAG GCGACTGGAG TGCCATGTCC GGTTTTCAAC AAACCATGCA AATGCTGAAT GAGGGCATCG TTCCCACTGC GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG CGCGCCATTA CCGAGTCCGG GCTGCGCGTT GGTGCGGATA TCTCGGTAGT GGGATACGAC GATACCGAAG ATAGCTCATG TTATATCCCG CCGTTAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCAGT CTCACTGGTG AAAAGAAAAA CCACCCTGGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG A
Details for CaalA.00629.a.HA11.PD00408
| PURIFICATION DATe: | 10/16/2015 |
| CONCENTRATION: | 20.43mg/ml |
| OBSERVED MW: | 71kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 10mM Tris pH 8.5, 20mM NaCl |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 20 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MHHHHHHHHE NLYFQGHDVN QYKQMYEQSI KDPQGFFGPL AKELLSWDHD FHTVKSGTLK NGDAAWFLGG ELNASYNCVD RHAFANPDKP ALICEADDEK DSHILTYGDL LREVSKVAGV LQSWGIKKGD TVAVYLPMNA QAIIAMLAIA RLGAAHSVIF AGFSAGSIKD RVNDASCKAL ITCDEGKRGG RTTNIKKLCD EALVDCPTVE KVLVYKRTNN PEIHLTEGRD YYWDVETAKF PGYLPPVSVN SEDPLFLLYT SGSTGTPKGV VHSTAGYLLG AALSTKYIFD IHPEDILFTA GDVGWITGHT YALYGPLLLG VPTIIFEGTP AYPDYGRFWQ IVEKHKATHF YVAPTALRLL RKAGEQEIAK YDLSSLRTLG SVGEPISPDI WEWYNEFVGK NQCHISDTYW QTESGSHLIA PLAGVVPNKP GSASYPFFGI DAALIDPVTG VEIEGNDAEG VLAIKDHWPS MARTVYKNHT KYMDTYMNPY PGYYFTGDGA ARDHDGYYWI RGRVDDVVNV SGHRLSTAEI EAALIEDKKV SEAAVVGIHD DITGQAVIAY VALKEGNSDE DSEGLRKELV LQVRKTIGPF AAPKSVIIVQ DLPKTRSGKI MRRILRKVSS NEADQLGDIS TLSNPQSVEG IISAFGA
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGCACCATC ACCACCACCA TCATCACGAG AATCTGTACT TCCAAGGCCA TGACGTTAAC CAGTACAAAC AAATGTATGA GCAGAGCATC AAAGATCCGC AGGGTTTCTT CGGCCCATTG GCGAAAGAAC TGCTGAGCTG GGATCATGAT TTTCATACGG TGAAGTCTGG TACTCTGAAG AATGGTGACG CGGCATGGTT CCTGGGTGGC GAGCTGAATG CCAGCTACAA CTGCGTAGAT CGTCACGCGT TCGCGAACCC GGACAAACCG GCACTGATTT GCGAGGCCGA CGATGAGAAA GACAGCCACA TCCTGACCTA CGGCGATTTG CTGCGTGAAG TGAGCAAAGT CGCCGGTGTG CTGCAGTCTT GGGGTATCAA GAAAGGCGAC ACGGTCGCGG TGTATCTGCC GATGAACGCG CAAGCGATCA TTGCGATGCT GGCCATTGCA CGTCTGGGTG CGGCGCACTC CGTGATTTTT GCGGGTTTTT CCGCTGGCAG CATTAAAGAT CGCGTTAATG ACGCTAGCTG TAAGGCACTG ATTACCTGTG ATGAGGGCAA GCGTGGTGGC CGCACCACCA ACATCAAGAA ACTGTGCGAC GAAGCGCTGG TCGACTGCCC GACGGTCGAA AAGGTCCTGG TGTACAAGCG CACCAACAAT CCAGAGATTC ATCTGACCGA GGGTCGTGAT TACTATTGGG ATGTCGAAAC CGCGAAGTTC CCGGGTTACT TGCCGCCTGT TTCAGTCAAC AGCGAAGATC CGCTGTTTCT GCTGTACACC AGCGGTAGCA CCGGTACGCC AAAAGGCGTG GTTCATAGCA CTGCAGGCTA TCTGCTGGGT GCAGCACTGA GCACTAAGTA CATCTTCGAT ATCCATCCGG AAGATATCCT GTTCACCGCC GGTGACGTGG GCTGGATCAC CGGTCATACT TACGCATTAT ACGGTCCGCT CCTGCTGGGC GTTCCGACCA TTATCTTCGA GGGTACGCCG GCATACCCGG ACTATGGTCG TTTCTGGCAA ATTGTTGAGA AGCACAAGGC CACCCACTTT TATGTTGCCC CGACCGCGCT GCGTCTGCTG CGTAAGGCTG GCGAGCAAGA AATTGCGAAA TATGACCTTA GCAGCCTGCG TACGCTGGGC TCGGTTGGCG AGCCGATTAG CCCGGATATT TGGGAGTGGT ATAATGAATT TGTTGGCAAG AACCAATGTC ACATCAGCGA TACCTACTGG CAGACCGAAT CCGGCAGCCA CTTGATCGCG CCACTGGCGG GTGTGGTCCC GAACAAACCG GGCAGCGCAT CGTATCCGTT CTTTGGTATT GACGCCGCAT TGATCGACCC TGTGACCGGT GTTGAGATTG AGGGTAATGA CGCTGAGGGC GTTCTGGCGA TCAAAGACCA CTGGCCGAGC ATGGCGCGTA CGGTCTATAA GAACCACACC AAATACATGG ACACGTATAT GAATCCGTAC CCGGGTTATT ACTTTACGGG TGACGGTGCT GCGCGCGACC ACGATGGCTA CTACTGGATT CGTGGCCGTG TTGACGATGT CGTGAACGTC AGCGGTCACC GTCTGAGCAC GGCCGAGATC GAAGCGGCGC TGATTGAAGA TAAGAAAGTC TCCGAGGCAG CTGTTGTGGG TATTCACGAC GACATTACGG GCCAAGCTGT TATTGCGTAC GTGGCATTGA AAGAGGGCAA TAGCGATGAG GACTCTGAGG GCCTGCGCAA AGAACTGGTG CTGCAAGTGC GCAAGACCAT CGGTCCGTTC GCAGCGCCGA AAAGCGTGAT CATTGTACAA GACTTGCCGA AAACGCGCAG CGGTAAGATC ATGCGTCGTA TCCTCCGTAA GGTCAGCTCC AATGAAGCGG ACCAGCTGGG TGATATTAGC ACGCTGAGCA ATCCGCAGTC TGTTGAGGGT ATCATCAGCG CCTTTGGTGC ATGATAAGCT TGCGGCCGCA CTCGAGCACC ACCACCACCA CCACTGAGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GAT