BuxeA.12800.z

Transcriptional regulator, LysR family

CENTER ID: BuxeA.12800.z
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.12800.z.A1.GE32112 Full length( BuxeA.12800.z ) 1 312
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.12800.z.A1.PS01123 Full length( BuxeA.12800.z ) 1 312

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.193
RefSeq: YP_556773.1
UniProt: Q146L8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDTLVSMKVF RHVVEVGSFV GAAERMEMSA AMASKHVMHL EQQLGARLLN RTTRRVAPTE AGREYYERLS QVLAELEEAE QAVGAASVVP QGRLRVSSLS AFGLSHVMAA VADYAAQYPQ VTVDMTLSDR VVELIDEGFD VAIRASPGGL KSSSLIARQI ATAHLVLCAS PAYLKRHGTP KTVADLARHN YLQYAGVSAL EIAPATGEAS SRVRLTGNLI VNHLEAQRVI VLQGAGIAML GTEVIGDDLA AGRLVPLLVD DVPPRELPIY VVYASRRHLS AKVRSFVDFL AERFANESLW PSLEQIKALA VR
NT Sequence
ATGGATACCC TCGTCAGCAT GAAAGTGTTC CGGCATGTGG TCGAAGTCGG CAGCTTTGTC GGCGCGGCCG AACGGATGGA GATGTCGGCG GCGATGGCGA GCAAACACGT GATGCATCTC GAACAGCAAC TCGGCGCGCG GCTCCTGAAC CGCACCACGC GGCGGGTCGC GCCCACCGAG GCGGGCCGCG AATATTACGA GCGTCTGAGC CAGGTGCTCG CCGAGCTCGA AGAGGCCGAA CAGGCGGTCG GCGCCGCGAG CGTCGTGCCG CAGGGGCGCT TGCGGGTGTC GTCGCTGTCG GCGTTCGGTT TGAGCCATGT GATGGCCGCG GTGGCGGACT ACGCCGCGCA GTATCCGCAG GTCACCGTCG ATATGACACT CTCCGACCGG GTGGTCGAAC TGATCGACGA AGGTTTCGAT GTCGCCATCC GGGCTTCGCC GGGCGGGCTC AAGTCTTCTT CATTGATCGC GCGGCAGATC GCGACCGCGC ATCTCGTGCT GTGCGCGTCG CCTGCCTATC TGAAACGGCA CGGCACGCCG AAAACCGTGG CCGACCTCGC GCGTCACAAC TATCTCCAGT ACGCGGGCGT GTCGGCGCTC GAAATCGCGC CGGCAACCGG CGAAGCTTCT TCGCGCGTAC GCCTGACGGG CAACCTGATC GTCAACCATC TCGAAGCGCA GCGTGTGATC GTGCTGCAGG GCGCGGGCAT CGCGATGCTC GGCACCGAAG TGATCGGCGA CGATCTGGCC GCGGGGCGCC TCGTGCCGCT GCTGGTTGAC GACGTGCCGC CGCGCGAGCT GCCGATTTAT GTGGTCTACG CGAGCCGCCG GCATCTGTCG GCCAAGGTGC GCTCGTTTGT CGACTTCCTC GCTGAGCGGT TCGCCAACGA GTCGCTGTGG CCGTCGCTCG AACAGATCAA GGCGTTGGCG GTGCGG
Details for BuxeA.12800.z.A1.GE32112
HARVESTED ON: 4/19/2011
SEQUENCED ON: 4/25/2011
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MGTLEAQTQG PGSMDTLVSM KVFRHVVEVG SFVGAAERME MSAAMASKHV MHLEQQLGAR LLNRTTRRVA PTEAGREYYE RLSQVLAELE EAEQAVGAAS VVPQGRLRVS SLSAFGLSHV MAAVADYAAQ YPQVTVDMTL SDRVVELIDE GFDVAIRASP GGLKSSSLIA RQIATAHLVL CASPAYLKRH GTPKTVADLA RHNYLQYAGV SALEIAPATG EASSRVRLTG NLIVNHLEAQ RVIVLQGAGI AMLGTEVIGD DLAAGRLVPL LVDDVPPREL PIYVVYASRR HLSAKVRSFV DFLAERFANE SLWPSLEQ
Validated NT Sequence
tctgttcgag cgacggccac agcgactcgt tggcgaaccg ctcagcgagg aagtcgacaa acgagcgcac cttggccgac agatgccggc ggctcgcgta gaccacataa atcggcagct cgcgcggcgg cacgtcgtca accagcagcg gcacgaggcg ccccgcggcc agatcgtcgc cgatcacttc ggtgccgagc atcgcgatgc ccgcgccctg cagcacgatc acacgctgcg cttcgagatg gttgacgatc aggttgcccg tcaggcgtac gcgcgaagaa gcttcgccgg ttgccggcgc gatttcgagc gccgacacgc ccgcgtactg gagatagttg tgacgcgcga ggtcggccac ggttttcggc gtgccgtgcc gtttcagata ggcaggcgac gcgcacagca cgagatgcgc ggtcgcgatc tgccgcgcga tcaatgaaga agacttgagc ccgcccggcg aagcccggat ggcgacatcg aaaccttcgt cgatcagttc gaccacccgg tcggagagtg tcatatcgac ggtgacctgc ggatactgcg cggcgtagtc cgccaccgcg gccatcacat ggctcaaacc gaacgccgac agcgacgaca cccgcaagcg cccctgcggc acgacgctcg cggcgccgac cgcctgttcg gcctcttcga gctcggcgag cacctggctc agacgctcgt aatattcgcg gcccgcctcg gtgggcgcga cccgccgcgt ggtgcggttc aggagccgcg cgccgagttg ctgttcgaga tgcatcacgt gtttgctcgc catcgccgcc gacatctcca tccgttcggc cgcgccgaca aagctgccga cttcgaccac atgccggaac actttcatgc tgacgagggt atccatcgaa ccaggaccct gggtctgagc ttccagggta cccatat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDTLVSMKV FRHVVEVGSF VGAAERMEMS AAMASKHVMH LEQQLGARLL NRTTRRVAPT EAGREYYERL SQVLAELEEA EQAVGAASVV PQGRLRVSSL SAFGLSHVMA AVADYAAQYP QVTVDMTLSD RVVELIDEGF DVAIRASPGG LKSSSLIARQ IATAHLVLCA SPAYLKRHGT PKTVADLARH NYLQYAGVSA LEIAPATGEA SSRVRLTGNL IVNHLEAQRV IVLQGAGIAM LGTEVIGDDL AAGRLVPLLV DDVPPRELPI YVVYASRRHL SAKVRSFVDF LAERFANESL WPSLEQIKAL AVR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggataccctc gtcagcatga aagtgttccg gcatgtggtc gaagtcggca gctttgtcgg cgcggccgaa cggatggaga tgtcggcggc gatggcgagc aaacacgtga tgcatctcga acagcaactc ggcgcgcggc tcctgaaccg caccacgcgg cgggtcgcgc ccaccgaggc gggccgcgaa tattacgagc gtctgagcca ggtgctcgcc gagctcgaag aggccgaaca ggcggtcggc gccgcgagcg tcgtgccgca ggggcgcttg cgggtgtcgt cgctgtcggc gttcggtttg agccatgtga tggccgcggt ggcggactac gccgcgcagt atccgcaggt caccgtcgat atgacactct ccgaccgggt ggtcgaactg atcgacgaag gtttcgatgt cgccatccgg gcttcgccgg gcgggctcaa gtcttcttca ttgatcgcgc ggcagatcgc gaccgcgcat ctcgtgctgt gcgcgtcgcc tgcctatctg aaacggcacg gcacgccgaa aaccgtggcc gacctcgcgc gtcacaacta tctccagtac gcgggcgtgt cggcgctcga aatcgcgccg gcaaccggcg aagcttcttc gcgcgtacgc ctgacgggca acctgatcgt caaccatctc gaagcgcagc gtgtgatcgt gctgcagggc gcgggcatcg cgatgctcgg caccgaagtg atcggcgacg atctggccgc ggggcgcctc gtgccgctgc tggttgacga cgtgccgccg cgcgagctgc cgatttatgt ggtctacgcg agccgccggc atctgtcggc caaggtgcgc tcgtttgtcg acttcctcgc tgagcggttc gccaacgagt cgctgtggcc gtcgctcgaa cagatcaagg cgttggcggt gcggtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BuxeA.12800.z.A1.PS01123
PURIFICATION DATe: 6/21/2011
CONCENTRATION: 7.31mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMDTLVSM KVFRHVVEVG SFVGAAERME MSAAMASKHV MHLEQQLGAR LLNRTTRRVA PTEAGREYYE RLSQVLAELE EAEQAVGAAS VVPQGRLRVS SLSAFGLSHV MAAVADYAAQ YPQVTVDMTL SDRVVELIDE GFDVAIRASP GGLKSSSLIA RQIATAHLVL CASPAYLKRH GTPKTVADLA RHNYLQYAGV SALEIAPATG EASSRVRLTG NLIVNHLEAQ RVIVLQGAGI AMLGTEVIGD DLAAGRLVPL LVDDVPPREL PIYVVYASRR HLSAKVRSFV DFLAERFANE SLWPSLEQ
Validated NT Sequence
tctgttcgag cgacggccac agcgactcgt tggcgaaccg ctcagcgagg aagtcgacaa acgagcgcac cttggccgac agatgccggc ggctcgcgta gaccacataa atcggcagct cgcgcggcgg cacgtcgtca accagcagcg gcacgaggcg ccccgcggcc agatcgtcgc cgatcacttc ggtgccgagc atcgcgatgc ccgcgccctg cagcacgatc acacgctgcg cttcgagatg gttgacgatc aggttgcccg tcaggcgtac gcgcgaagaa gcttcgccgg ttgccggcgc gatttcgagc gccgacacgc ccgcgtactg gagatagttg tgacgcgcga ggtcggccac ggttttcggc gtgccgtgcc gtttcagata ggcaggcgac gcgcacagca cgagatgcgc ggtcgcgatc tgccgcgcga tcaatgaaga agacttgagc ccgcccggcg aagcccggat ggcgacatcg aaaccttcgt cgatcagttc gaccacccgg tcggagagtg tcatatcgac ggtgacctgc ggatactgcg cggcgtagtc cgccaccgcg gccatcacat ggctcaaacc gaacgccgac agcgacgaca cccgcaagcg cccctgcggc acgacgctcg cggcgccgac cgcctgttcg gcctcttcga gctcggcgag cacctggctc agacgctcgt aatattcgcg gcccgcctcg gtgggcgcga cccgccgcgt ggtgcggttc aggagccgcg cgccgagttg ctgttcgaga tgcatcacgt gtttgctcgc catcgccgcc gacatctcca tccgttcggc cgcgccgaca aagctgccga cttcgaccac atgccggaac actttcatgc tgacgagggt atccatcgaa ccaggaccct gggtctgagc ttccagggta cccatat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDTLVSMKV FRHVVEVGSF VGAAERMEMS AAMASKHVMH LEQQLGARLL NRTTRRVAPT EAGREYYERL SQVLAELEEA EQAVGAASVV PQGRLRVSSL SAFGLSHVMA AVADYAAQYP QVTVDMTLSD RVVELIDEGF DVAIRASPGG LKSSSLIARQ IATAHLVLCA SPAYLKRHGT PKTVADLARH NYLQYAGVSA LEIAPATGEA SSRVRLTGNL IVNHLEAQRV IVLQGAGIAM LGTEVIGDDL AAGRLVPLLV DDVPPRELPI YVVYASRRHL SAKVRSFVDF LAERFANESL WPSLEQIKAL AVR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATACCCTC GTCAGCATGA AAGTGTTCCG GCATGTGGTC GAAGTCGGCA GCTTTGTCGG CGCGGCCGAA CGGATGGAGA TGTCGGCGGC GATGGCGAGC AAACACGTGA TGCATCTCGA ACAGCAACTC GGCGCGCGGC TCCTGAACCG CACCACGCGG CGGGTCGCGC CCACCGAGGC GGGCCGCGAA TATTACGAGC GTCTGAGCCA GGTGCTCGCC GAGCTCGAAG AGGCCGAACA GGCGGTCGGC GCCGCGAGCG TCGTGCCGCA GGGGCGCTTG CGGGTGTCGT CGCTGTCGGC GTTCGGTTTG AGCCATGTGA TGGCCGCGGT GGCGGACTAC GCCGCGCAGT ATCCGCAGGT CACCGTCGAT ATGACACTCT CCGACCGGGT GGTCGAACTG ATCGACGAAG GTTTCGATGT CGCCATCCGG GCTTCGCCGG GCGGGCTCAA GTCTTCTTCA TTGATCGCGC GGCAGATCGC GACCGCGCAT CTCGTGCTGT GCGCGTCGCC TGCCTATCTG AAACGGCACG GCACGCCGAA AACCGTGGCC GACCTCGCGC GTCACAACTA TCTCCAGTAC GCGGGCGTGT CGGCGCTCGA AATCGCGCCG GCAACCGGCG AAGCTTCTTC GCGCGTACGC CTGACGGGCA ACCTGATCGT CAACCATCTC GAAGCGCAGC GTGTGATCGT GCTGCAGGGC GCGGGCATCG CGATGCTCGG CACCGAAGTG ATCGGCGACG ATCTGGCCGC GGGGCGCCTC GTGCCGCTGC TGGTTGACGA CGTGCCGCCG CGCGAGCTGC CGATTTATGT GGTCTACGCG AGCCGCCGGC ATCTGTCGGC CAAGGTGCGC TCGTTTGTCG ACTTCCTCGC TGAGCGGTTC GCCAACGAGT CGCTGTGGCC GTCGCTCGAA CAGATCAAGG CGTTGGCGGT GCGGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA