SSGCID: Target Page

BuxeA.12800.x

Transcriptional regulator, LysR family

CENTER ID:	BuxeA.12800.x
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.12800.x.A1.GE32110	Full length( BuxeA.12800.x )		1	305

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.12800.x.A1.PS01206	Full length( BuxeA.12800.x )		1	305

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.1022
RefSeq:	YP_557590.1
UniProt:	Q143F1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRFEAMEIF TRVVEANSFT KVSESLDLPR AKVSRTIQAL EEHVGVRLLN RSTRQVSVTE DGALFYERCV RILAEVAEAE SSLSNKRENP AGTIRVDTSG TLARALLLPA LDDFYRQYPE IDVRLGLADR NIDLIQDGVD CVIRMGTPEE SSLVARRIGQ ARIVTCASPA YLEKYGEPTT LEALSEHRAV NYVSARTGRT FPFEYEVDGE LAKVTLKSVL AVNDGSVYIG AAVLGHGIIQ PSRFMVSDLI NQGALKEILT TYTSPGTPLS VLYAHRRNLS SRLRAFIEWV TELARNNPDL HIAEQ

NT Sequence

ATGGACCGTT TCGAAGCGAT GGAGATATTT ACGCGTGTGG TCGAGGCGAA CAGCTTCACC AAAGTATCCG AGTCGCTCGA CCTGCCGCGC GCCAAAGTCA GCCGCACCAT CCAGGCGCTG GAGGAACACG TCGGCGTGCG TCTGCTGAAC CGTTCGACGC GCCAGGTCAG CGTCACCGAA GACGGCGCGC TGTTCTACGA GCGCTGCGTG CGCATCCTCG CCGAAGTCGC CGAGGCCGAG TCGTCGCTGT CGAACAAGCG CGAAAATCCG GCCGGCACGA TCCGCGTGGA TACGTCCGGC ACGCTGGCCC GCGCGCTGCT GCTGCCCGCG CTCGACGACT TCTACCGGCA ATACCCCGAG ATCGACGTGC GGCTCGGCCT CGCCGATCGC AACATCGACC TGATTCAGGA CGGCGTGGAT TGCGTGATCC GCATGGGCAC GCCGGAAGAG TCGAGTCTCG TGGCGCGGCG GATCGGTCAG GCGCGCATCG TCACATGCGC GTCGCCGGCG TATCTGGAGA AATACGGCGA GCCGACCACG CTCGAAGCGT TGAGCGAACA TCGCGCGGTC AATTACGTGT CCGCGCGCAC GGGCAGAACG TTTCCATTCG AATACGAAGT GGACGGCGAA CTCGCCAAGG TCACGCTGAA AAGCGTGCTG GCGGTAAACG ACGGCTCCGT GTACATCGGC GCGGCCGTGC TCGGTCACGG CATCATCCAG CCGTCGCGCT TCATGGTCTC CGACCTGATC AACCAGGGCG CGCTGAAGGA AATCCTCACC ACCTACACGA GCCCCGGCAC GCCGCTATCG GTGCTCTACG CGCATCGCCG CAATCTGAGT TCGCGGCTGC GCGCGTTCAT CGAATGGGTG ACCGAGCTGG CGCGCAACAA TCCGGATCTG CATATTGCGG AGCAG

Details for BuxeA.12800.x.A1.GE32110

HARVESTED ON:	4/19/2011
SEQUENCED ON:	4/25/2011
EXPECTED MW:	36kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMDRFEAM EIFTRVVEAN SFTKVSESLD LPRAKVSRTI QALEEHVGVR LLNRSTRQVS VTEDGALFYE RCVRILAEVA EAESSLSNKR ENPAGTIRVD TSGTLARALL LPALDDFYRQ YPEIDVRLGL ADRNIDLIQD GVDCVIRMGT PEESSLVARR IGQARIVTCA SPAYLEKYGE PTTLEALSEH RAVNYVSART GRTFPFEYEV DGELAKVTLK SVLAVNDGSV YIGAAVLGHG IIQPSRFMVS DLINQGALKE ILTTYTSPGT PLSVLYAHRR NLSSRLRAFI EWVTELARNN PDLHIA

Validated NT Sequence

ccgcaatatg cagatccgga ttgttgcgcg ccagctcggt cacccattcg atgaacgcgc gcagccgcga actcagattg cggcgatgcg cgtagagcac cgatagcggc gtgccggggc tcgtgtaggt ggtgaggatt tccttcagcg cgccctggtt gatcaggtcg gagaccatga agcgcgacgg ctggatgatg ccgtgaccga gcacggccgc gccgatgtac acggagccgt cgtttaccgc cagcacgctt ttcagcgtga ccttggcgag ttcgccgtcc acttcgtatt cgaatggaaa cgttctgccc gtgcgcgcgg acacgtaatt gaccgcgcga tgttcgctca acgcttcgag cgtggtcggc tcgccgtatt tctccagata cgccggcgac gcgcatgtga cgatgcgcgc ctgaccgatc cgccgcgcca cgagactcga ctcttccggc gtgcccatgc ggatcacgca atccacgccg tcctgaatca ggtcgatgtt gcgatcggcg aggccgagcc gcacgtcgat ctcggggtat tgccggtaga agtcgtcgag cgcgggcagc agcagcgcgc gggccagcgt gccggacgta tccacgcgga tcgtgccggc cggattttcg cgcttgttcg acagcgacga ctcggcctcg gcgacttcgg cgaggatgcg cacgcagcgc tcgtagaaca gcgcgccgtc ttcggtgacg ctgacctggc gcgtcgaacg gttcagcaga cgcacgccga cgtgttcctc cagcgcctgg atggtgcggc tgactttggc gcgcggcagg tcgagcgact cggatacttt ggtgaagctg ttcgcctcga ccacacgcgt aaatatctcc atcgcttcga aacggtccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatnann ca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDRFEAMEI FTRVVEANSF TKVSESLDLP RAKVSRTIQA LEEHVGVRLL NRSTRQVSVT EDGALFYERC VRILAEVAEA ESSLSNKREN PAGTIRVDTS GTLARALLLP ALDDFYRQYP EIDVRLGLAD RNIDLIQDGV DCVIRMGTPE ESSLVARRIG QARIVTCASP AYLEKYGEPT TLEALSEHRA VNYVSARTGR TFPFEYEVDG ELAKVTLKSV LAVNDGSVYI GAAVLGHGII QPSRFMVSDL INQGALKEIL TTYTSPGTPL SVLYAHRRNL SSRLRAFIEW VTELARNNPD LHIAEQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccgtttc gaagcgatgg agatatttac gcgtgtggtc gaggcgaaca gcttcaccaa agtatccgag tcgctcgacc tgccgcgcgc caaagtcagc cgcaccatcc aggcgctgga ggaacacgtc ggcgtgcgtc tgctgaaccg ttcgacgcgc caggtcagcg tcaccgaaga cggcgcgctg ttctacgagc gctgcgtgcg catcctcgcc gaagtcgccg aggccgagtc gtcgctgtcg aacaagcgcg aaaatccggc cggcacgatc cgcgtggata cgtccggcac gctggcccgc gcgctgctgc tgcccgcgct cgacgacttc taccggcaat accccgagat cgacgtgcgg ctcggcctcg ccgatcgcaa catcgacctg attcaggacg gcgtggattg cgtgatccgc atgggcacgc cggaagagtc gagtctcgtg gcgcggcgga tcggtcaggc gcgcatcgtc acatgcgcgt cgccggcgta tctggagaaa tacggcgagc cgaccacgct cgaagcgttg agcgaacatc gcgcggtcaa ttacgtgtcc gcgcgcacgg gcagaacgtt tccattcgaa tacgaagtgg acggcgaact cgccaaggtc acgctgaaaa gcgtgctggc ggtaaacgac ggctccgtgt acatcggcgc ggccgtgctc ggtcacggca tcatccagcc gtcgcgcttc atggtctccg acctgatcaa ccagggcgcg ctgaaggaaa tcctcaccac ctacacgagc cccggcacgc cgctatcggt gctctacgcg catcgccgca atctgagttc gcggctgcgc gcgttcatcg aatgggtgac cgagctggcg cgcaacaatc cggatctgca tattgcggag cagtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for BuxeA.12800.x.A1.PS01206

PURIFICATION DATe:	9/14/2011
CONCENTRATION:	3.79mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACCGTTTC GAAGCGATGG AGATATTTAC GCGTGTGGTC GAGGCGAACA GCTTCACCAA AGTATCCGAG TCGCTCGACC TGCCGCGCGC CAAAGTCAGC CGCACCATCC AGGCGCTGGA GGAACACGTC GGCGTGCGTC TGCTGAACCG TTCGACGCGC CAGGTCAGCG TCACCGAAGA CGGCGCGCTG TTCTACGAGC GCTGCGTGCG CATCCTCGCC GAAGTCGCCG AGGCCGAGTC GTCGCTGTCG AACAAGCGCG AAAATCCGGC CGGCACGATC CGCGTGGATA CGTCCGGCAC GCTGGCCCGC GCGCTGCTGC TGCCCGCGCT CGACGACTTC TACCGGCAAT ACCCCGAGAT CGACGTGCGG CTCGGCCTCG CCGATCGCAA CATCGACCTG ATTCAGGACG GCGTGGATTG CGTGATCCGC ATGGGCACGC CGGAAGAGTC GAGTCTCGTG GCGCGGCGGA TCGGTCAGGC GCGCATCGTC ACATGCGCGT CGCCGGCGTA TCTGGAGAAA TACGGCGAGC CGACCACGCT CGAAGCGTTG AGCGAACATC GCGCGGTCAA TTACGTGTCC GCGCGCACGG GCAGAACGTT TCCATTCGAA TACGAAGTGG ACGGCGAACT CGCCAAGGTC ACGCTGAAAA GCGTGCTGGC GGTAAACGAC GGCTCCGTGT ACATCGGCGC GGCCGTGCTC GGTCACGGCA TCATCCAGCC GTCGCGCTTC ATGGTCTCCG ACCTGATCAA CCAGGGCGCG CTGAAGGAAA TCCTCACCAC CTACACGAGC CCCGGCACGC CGCTATCGGT GCTCTACGCG CATCGCCGCA ATCTGAGTTC GCGGCTGCGC GCGTTCATCG AATGGGTGAC CGAGCTGGCG CGCAACAATC CGGATCTGCA TATTGCGGAG CAGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA