SSGCID: Target Page

BuxeA.12800.w

Transcriptional regulator, LysR family

CENTER ID:	BuxeA.12800.w
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.12800.w.A1.GE32109	Full length( BuxeA.12800.w )		1	301

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.1598
RefSeq:	YP_558130.1
UniProt:	Q141B1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSERVDAPIN EIAVFVAVAQ TGSFTRAAEE IGSSKSNVGK AVQRLEARLG SQLFQRTTRA VRLTEDGETY LLAAQEALES LREAEQQLAS RRAEPAGRVK IDLPAGFGRL LLPSFIQLRE KYPKLTVEMA LTDRMSDPVG EGWDIVVRIG ALPDDSEFTV RKLCDLRLGL YASPEYLARR GDVKTLAELT DHDAIVFRGP TGRLRSWTTR ENDTVREIVP HPVLVLADGQ ALVEAAVAGF GITQIYDRVA LPHVRAGRLV HLLPEADVDG PPVHAIISLG QKMTAKTRAA VNHLAETLQQ G

NT Sequence

ATGAGCGAGC GGGTAGATGC ACCCATAAAT GAAATCGCGG TATTTGTGGC GGTTGCCCAA ACCGGCAGCT TTACGCGCGC GGCCGAAGAA ATCGGCAGCA GCAAGTCCAA TGTGGGTAAA GCGGTCCAGC GCCTCGAAGC CCGACTCGGC TCCCAGCTTT TTCAGCGCAC CACTCGTGCG GTGCGGCTGA CCGAAGACGG CGAGACGTAT CTGCTTGCCG CGCAGGAAGC GCTCGAATCC CTTCGCGAAG CGGAGCAGCA ACTCGCGTCG AGGCGGGCCG AGCCCGCCGG GCGCGTCAAG ATTGACCTGC CCGCGGGCTT CGGACGTCTG TTGCTGCCGA GTTTTATCCA GCTTCGTGAA AAGTATCCCA AGCTCACGGT GGAGATGGCC CTGACTGACC GGATGTCCGA CCCGGTCGGG GAGGGCTGGG ATATTGTCGT TCGCATTGGT GCGCTACCTG ACGACAGTGA ATTTACCGTG CGCAAGCTGT GCGACCTGAG GCTAGGTCTG TATGCGTCGC CAGAGTATCT CGCGCGCCGG GGTGACGTGA AGACGCTCGC GGAGTTGACT GACCACGACG CAATAGTTTT CCGTGGTCCA ACGGGGCGAC TACGTTCATG GACAACGCGA GAAAACGACA CCGTGCGGGA AATTGTCCCG CACCCTGTTC TCGTGCTTGC CGACGGTCAG GCTCTCGTCG AGGCGGCGGT GGCTGGCTTC GGCATTACTC AGATTTACGA CCGTGTCGCG CTTCCACATG TGCGTGCCGG TCGCCTCGTG CACCTGCTAC CCGAAGCGGA TGTAGACGGG CCGCCCGTGC ATGCCATCAT TTCGCTCGGG CAGAAGATGA CGGCCAAGAC ACGGGCGGCG GTGAATCACC TCGCCGAAAC GCTACAGCAG GGA

Details for BuxeA.12800.w.A1.GE32109

HARVESTED ON:	4/19/2011
SEQUENCED ON:	4/25/2011
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMSERVDA PINEIAVFVA VAQTGSFTRA AEEIGSSKSN VGKAVQRLEA RLGSQLFQRT TRAVRLTEDG ETYLLAAQEA LESLREAEQQ LASRRAEPAG RVKIDLPAGF GRLLLPSFIQ LREKYPKLTV EMALTDRMSD PVGEGWDIVV RIGALPDDSE FTVRKLCDLR LGLYASPEYL ARRGDVKTLA ELTDHDAIVF RGPTGRLRSW TTRENDTVRE IVPHPVLVLA DGQALVEAAV AGFGITQIYD RVALPHVRAG RLVHLLPEAD VDGPPVHAII SLGQKMTAKT RAAVNHLAET L

Validated NT Sequence

ctgtagcgtt tcggcgaggt gattcaccgc cgcccgtgtc ttggccgtca tcttctgccc gagcgaaatg atggcatgca cgggcggccc gtctacatcc gcttcgggta gcaggtgcac gaggcgaccg gcacgcacat gtggaagcgc gacacggtcg taaatctgag taatgccgaa gccagccacc gccgcctcga cgagagcctg accgtcggca agcacgagaa cagggtgcgg gacaatttcc cgcacggtgt cgttttctcg cgttgtccat gaacgtagtc gccccgttgg accacggaaa actattgcgt cgtggtcagt caactccgcg agcgtcttca cgtcaccccg gcgcgcgaga tactctggcg acgcatacag acctagcctc aggtcgcaca gcttgcgcac ggtaaattca ctgtcgtcag gtagcgcacc aatgcgaacg acaatatccc agccctcccc gaccgggtcg gacatccggt cagtcagggc catctccacc gtgagcttgg gatacttttc acgaagctgg ataaaactcg gcagcaacag acgtccgaag cccgcgggca ggtcaatctt gacgcgcccg gcgggctcgg cccgcctcga cgcgagttgc tgctccgctt cgcgaaggga ttcgagcgct tcctgcgcgg caagcagata cgtctcgccg tcttcggtca gccgcaccgc acgagtggtg cgctgaaaaa gctgggagcc gagtcgggct tcgaggcgct ggaccgcttt acccacattg gacttgctgc tgccgatttc ttcggccgcg cgcgtaaagc tgccggtttg ggcaaccgcc acaaataccg cgatttcatt tatgggtgca tctacccgct cgctcatcga accaggaccc tgggtctgag cttccagggt acccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSERVDAPI NEIAVFVAVA QTGSFTRAAE EIGSSKSNVG KAVQRLEARL GSQLFQRTTR AVRLTEDGET YLLAAQEALE SLREAEQQLA SRRAEPAGRV KIDLPAGFGR LLLPSFIQLR EKYPKLTVEM ALTDRMSDPV GEGWDIVVRI GALPDDSEFT VRKLCDLRLG LYASPEYLAR RGDVKTLAEL TDHDAIVFRG PTGRLRSWTT RENDTVREIV PHPVLVLADG QALVEAAVAG FGITQIYDRV ALPHVRAGRL VHLLPEADVD GPPVHAIISL GQKMTAKTRA AVNHLAETLQ QG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgagcgg gtagatgcac ccataaatga aatcgcggta tttgtggcgg ttgcccaaac cggcagcttt acgcgcgcgg ccgaagaaat cggcagcagc aagtccaatg tgggtaaagc ggtccagcgc ctcgaagccc gactcggctc ccagcttttt cagcgcacca ctcgtgcggt gcggctgacc gaagacggcg agacgtatct gcttgccgcg caggaagcgc tcgaatccct tcgcgaagcg gagcagcaac tcgcgtcgag gcgggccgag cccgccgggc gcgtcaagat tgacctgccc gcgggcttcg gacgtctgtt gctgccgagt tttatccagc ttcgtgaaaa gtatcccaag ctcacggtgg agatggccct gactgaccgg atgtccgacc cggtcgggga gggctgggat attgtcgttc gcattggtgc gctacctgac gacagtgaat ttaccgtgcg caagctgtgc gacctgaggc taggtctgta tgcgtcgcca gagtatctcg cgcgccgggg tgacgtgaag acgctcgcgg agttgactga ccacgacgca atagttttcc gtggtccaac ggggcgacta cgttcatgga caacgcgaga aaacgacacc gtgcgggaaa ttgtcccgca ccctgttctc gtgcttgccg acggtcaggc tctcgtcgag gcggcggtgg ctggcttcgg cattactcag atttacgacc gtgtcgcgct tccacatgtg cgtgccggtc gcctcgtgca cctgctaccc gaagcggatg tagacgggcc gcccgtgcat gccatcattt cgctcgggca gaagatgacg gccaagacac gggcggcggt gaatcacctc gccgaaacgc tacagcaggg ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa