SSGCID: Target Page

BuxeA.12800.v

Transcriptional regulator, LysR family

CENTER ID:	BuxeA.12800.v
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.12800.v.A1.GE32108	Full length( BuxeA.12800.v )		1	342

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.1604
RefSeq:	YP_558137.1
UniProt:	Q141A4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDKLQAMQVF TRVVDTNSFS GAADSLHMTR SSVTTIIQAL EAYLKVRLLN RTTRRISLTP DGAAYYERCS RILADVEDSE SSFSRASAPR GKLKVDMPGS IGRLLIVPAL DEFHARYPDI ELMLGVGDRA VDLIQDSVDC AIRMGPMQDS TLVARRVGVS EFVTVASPDY IARNGEPVSL AELESHTAVN YFSSRNGRIV EMDFVVDGKP VDVRMRSKLA ANDGEAYLQC GLRGLGLIQV PHFLALPHLE SGALVEVLGN CRPSPFPISA VYPQNRHLSP QVRVFVEWVA ELFENCHLLR ADNVLPSGIA QYVKHRQSST AAGRVLESAR APGTRSRDLA PM

NT Sequence

ATGGACAAAC TTCAAGCGAT GCAGGTCTTC ACGCGGGTAG TCGACACCAA CAGCTTTTCC GGTGCCGCCG ACTCCCTGCA CATGACGCGT TCGTCAGTCA CGACCATCAT CCAGGCTCTT GAGGCGTACC TCAAGGTACG CCTGCTCAAT CGCACGACCC GCCGTATCAG CCTCACTCCG GACGGTGCTG CGTACTATGA GCGCTGCTCG CGCATTCTCG CCGATGTCGA GGATTCGGAG AGCTCGTTTT CGCGCGCGTC AGCTCCGCGC GGCAAGCTCA AAGTCGATAT GCCAGGGTCT ATCGGCCGTC TCCTGATCGT CCCCGCGCTG GACGAGTTCC ACGCGCGATA TCCGGACATC GAACTGATGC TCGGCGTCGG CGACAGGGCC GTAGACCTGA TTCAGGACAG TGTTGACTGT GCCATCCGCA TGGGGCCGAT GCAGGACTCG ACGCTGGTAG CAAGAAGGGT TGGCGTCTCG GAGTTCGTCA CCGTGGCGAG CCCGGACTAC ATTGCACGCA ATGGCGAGCC GGTGTCGCTC GCCGAGCTCG AATCGCACAC AGCCGTCAAC TACTTCTCCA GCCGCAATGG CCGCATCGTG GAGATGGACT TCGTCGTCGA CGGCAAGCCC GTGGACGTTC GCATGCGCTC GAAGCTCGCC GCCAATGACG GCGAGGCATA CCTGCAATGC GGTCTAAGGG GTCTCGGGCT CATCCAGGTC CCTCACTTCC TTGCGCTTCC TCATCTGGAA TCGGGTGCTC TGGTCGAGGT ACTCGGCAAT TGCCGTCCAA GTCCGTTTCC TATTTCAGCG GTGTATCCGC AAAACCGTCA CCTCTCTCCA CAGGTCCGCG TATTTGTCGA ATGGGTTGCC GAACTGTTCG AGAACTGTCA TCTGCTGCGT GCGGACAACG TGCTGCCCTC GGGTATCGCC CAATACGTCA AGCATCGCCA GTCGTCCACC GCTGCGGGCC GCGTGCTGGA GTCAGCGCGC GCGCCGGGTA CGCGCAGCAG GGATCTGGCG CCTATG

Details for BuxeA.12800.v.A1.GE32108

HARVESTED ON:	4/19/2011
SEQUENCED ON:	4/25/2011
EXPECTED MW:	39kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	73
PERCENT COVERAGE:	93

Validated AA Sequence

MXXHHHHXXX TLEAQTQGPG SMXKLQAMQV FTRVVDTNSF SGAADSLHMT RSSVTTIIQA LEAYLKVRLL NRTTRRISLT PDGAAYYERC SRILADVEDS ESSFSRASAP RGKLKVDMPG SIGRLLIVPA LDEFHARYPD IELMLGVGDR AVDLIQDSVD CAIRMGPMQD STLVARRVGV SEFVTVASPD YIARNGEPVS LAELESHTAV NYFSSRNGRI VEMDFVVXGK PVDVRMRSKL AAXDGXAYLQ CGLXXSGSSX SLTSXPSSSG XGCSXXGTXX XXSXSVXXXS XXXXXXHXXX GXXXXXGXXX XXXXXXXXXX XXXXXXXXXX RXXXXXXXXX

Validated NT Sequence

cnnnnnnnnn nnnngnccat ggntcancac catcaccatn nnntgnntac cctggaagct cagacccagg gtcctggttc natgnacaaa cttcaagcga tgcaggtctt cacgcgggta gtcgacacca acagcttttc cggtgccgcc gactccctgc acatgacgcg ttcgtcagtc acgaccatca tccaggctct tgaggcgtac ctcaaggtac gcctgctcaa tcgcacgacc cgccgtatca gcctcactcc ggacggtgct gcgtactatg agcgctgctc gcgcattctc gccgatgtcg aggattcgga gagctcgttt tcgcgcgcgt cagctccgcg cggcaagctc aaagtcgata tgccagggtc tatcggccgt ctcctgatcg tccccgcgct ggacgagttc cacgcgcgat atccggacat cgaactgatg ctcggcgtcg gcgacagggc cgtagacctg attcaggaca gtgttgactg tgccatccgc atggggccga tgcaggactc gacgctggta gcaagaaggg ttggcgtctc ggagttcgtc accgtggcga gcccggacta cattgcacgc aatggcgagc cggtgtcgct cgccgagctc gaatcgcaca cagccgtcaa ctacttctcc agccgcaatg gccgcatcgt ggagatggac ttcgtcgtcn acggnaagcc cgtggacgtt cgcatgcgct cgaagctcgc cgccnatgac ggngangcat acctgcaatg cggtctannn ntctcgggct catccnngtc cctcacttcn ntgccttcct catctggaan nggntgctct ngtcnaggta ctcnnnaatn gncgtccnnn tccgtttnct anntcagcnn gtntcnncnn nnnnntcacn ctntcncagg nnnnnnnnnn tnnnnnggnn gnnnantgtn nannnnntna ntgnntgnnn nannnnntnn ntnnnnnnnn nnnnnnnntn annatngncg nngtcnnnnn nnnnnnnnnn nctnnnnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDKLQAMQV FTRVVDTNSF SGAADSLHMT RSSVTTIIQA LEAYLKVRLL NRTTRRISLT PDGAAYYERC SRILADVEDS ESSFSRASAP RGKLKVDMPG SIGRLLIVPA LDEFHARYPD IELMLGVGDR AVDLIQDSVD CAIRMGPMQD STLVARRVGV SEFVTVASPD YIARNGEPVS LAELESHTAV NYFSSRNGRI VEMDFVVDGK PVDVRMRSKL AANDGEAYLQ CGLRGLGLIQ VPHFLALPHL ESGALVEVLG NCRPSPFPIS AVYPQNRHLS PQVRVFVEWV AELFENCHLL RADNVLPSGI AQYVKHRQSS TAAGRVLESA RAPGTRSRDL APM

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaaactt caagcgatgc aggtcttcac gcgggtagtc gacaccaaca gcttttccgg tgccgccgac tccctgcaca tgacgcgttc gtcagtcacg accatcatcc aggctcttga ggcgtacctc aaggtacgcc tgctcaatcg cacgacccgc cgtatcagcc tcactccgga cggtgctgcg tactatgagc gctgctcgcg cattctcgcc gatgtcgagg attcggagag ctcgttttcg cgcgcgtcag ctccgcgcgg caagctcaaa gtcgatatgc cagggtctat cggccgtctc ctgatcgtcc ccgcgctgga cgagttccac gcgcgatatc cggacatcga actgatgctc ggcgtcggcg acagggccgt agacctgatt caggacagtg ttgactgtgc catccgcatg gggccgatgc aggactcgac gctggtagca agaagggttg gcgtctcgga gttcgtcacc gtggcgagcc cggactacat tgcacgcaat ggcgagccgg tgtcgctcgc cgagctcgaa tcgcacacag ccgtcaacta cttctccagc cgcaatggcc gcatcgtgga gatggacttc gtcgtcgacg gcaagcccgt ggacgttcgc atgcgctcga agctcgccgc caatgacggc gaggcatacc tgcaatgcgg tctaaggggt ctcgggctca tccaggtccc tcacttcctt gcgcttcctc atctggaatc gggtgctctg gtcgaggtac tcggcaattg ccgtccaagt ccgtttccta tttcagcggt gtatccgcaa aaccgtcacc tctctccaca ggtccgcgta tttgtcgaat gggttgccga actgttcgag aactgtcatc tgctgcgtgc ggacaacgtg ctgccctcgg gtatcgccca atacgtcaag catcgccagt cgtccaccgc tgcgggccgc gtgctggagt cagcgcgcgc gccgggtacg cgcagcaggg atctggcgcc tatgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa