BuxeA.12800.t

Transcriptional regulator, LysR family

CENTER ID: BuxeA.12800.t
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.12800.t.A1.GE32106 Full length( BuxeA.12800.t ) 1 298
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.1805
RefSeq: YP_558322.1
UniProt: Q140G9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRIDAMRLL VRIVERGSFA AAARDLDVPR SSVTQGIQEL ERRLGAQLLQ RTTRQVRPTL DGEAFYRRCL GIIADVEEAE AAFNGAKPSG LVRVDVHGTL ARYFMMPALP SFLDTYPDIQ VQISEADRLV DLTRDGVDCV LRVGELPDSS LVGRRIAMLK QGTFASPEYL ARHGVPQHPA QLEGHRMVGY LSQITGEIIP LEFLVAGKRQ SMTLPASVMV TGAETNISAA RLGLGLVQVP RYRVADDLSA GTLAEVLGKF PPRPLPVHVL YTRTPQLSPR VRVFVDWLAK EFAARLSD
NT Sequence
ATGGATCGGA TCGACGCCAT GCGCCTGCTC GTCCGGATCG TGGAGCGCGG CAGTTTTGCT GCCGCCGCGC GGGATCTGGA CGTGCCGCGT TCGAGCGTGA CGCAGGGGAT TCAGGAACTG GAGCGGCGCC TCGGCGCGCA GTTGCTTCAG CGGACGACGC GGCAGGTGCG GCCGACGCTC GACGGCGAGG CTTTCTACCG GCGTTGTCTT GGCATCATTG CGGATGTCGA GGAAGCCGAA GCCGCATTCA ACGGCGCAAA GCCGTCGGGT CTGGTGCGAG TCGACGTTCA CGGCACACTC GCGCGATATT TCATGATGCC AGCGTTACCG AGCTTTCTGG ACACCTATCC GGATATCCAG GTGCAGATCA GCGAGGCGGA TCGTCTGGTG GATCTGACTC GCGATGGCGT CGACTGCGTG CTTCGGGTGG GCGAACTTCC GGACAGCTCA CTGGTCGGGC GCCGGATTGC CATGCTGAAG CAGGGGACGT TTGCCAGCCC GGAATATCTG GCGCGGCACG GCGTGCCGCA GCATCCTGCT CAACTTGAGG GGCACCGCAT GGTTGGCTAC CTGTCCCAGA TCACGGGCGA GATTATCCCG CTGGAATTCC TGGTGGCCGG GAAGCGCCAG AGCATGACAT TGCCGGCCAG CGTCATGGTG ACGGGCGCTG AAACGAACAT TAGCGCCGCC CGGCTGGGAC TGGGTCTGGT CCAGGTGCCG CGCTACCGCG TGGCCGATGA TCTCTCGGCG GGAACGCTGG CGGAAGTGCT GGGGAAATTT CCCCCGCGGC CGCTGCCTGT CCACGTGCTG TACACGCGCA CGCCCCAGCT CTCGCCGCGC GTGCGCGTGT TCGTGGACTG GCTGGCGAAG GAATTTGCCG CCAGATTATC CGAC
Details for BuxeA.12800.t.A1.GE32106
HARVESTED ON: 4/19/2011
SEQUENCED ON: 4/25/2011
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MXTLEAQTQG PGSMDRIDAM RLLVRIVERG SFAAAARDLD VPRSSVTQGI QELERRLGAQ LLQRTTRQVR PTLDGEAFYR RCLGIIADVE EAEAAFNGAK PSGLVRVDVH GTLARYFMMP ALPSFLDTYP DIQVQISEAD RLVDLTRDGV DCVLRVGELP DSSLVGRRIA MLKQGTFASP EYLARHGVPQ HPAQLEGHRM VGYLSQITGE IIPLEFLVAG KRQSMTLPAS VMVTGAETNI SAARLGLGLV QVPRYRVADD LSAGTLAEVL GKFPPRPLPV HVLYTRTPQL SPRVRVFVDW LAKEFAARLS D
Validated NT Sequence
ttattagtcg gataatctgg cggcaaattc cttcgccagc cagtccacga acacgcgcac gcgcggcgag agctggggcg tgcgcgtgta cagcacgtgg acaggcagcg gccgcggggg aaatttcccc agcacttccg ccagcgttcc cgccgagaga tcatcggcca cgcggtagcg cggcacctgg accagaccca gtcccagccg ggcggcgcta atgttcgttt cagcgcccgt caccatgacg ctggccggca atgtcatgct ctggcgcttc ccggccacca ggaattccag cgggataatc tcgcccgtga tctgggacag gtagccaacc atgcggtgcc cctcaagttg agcaggatgc tgcggcacgc cgtgccgcgc cagatattcc gggctggcaa acgtcccctg cttcagcatg gcaatccggc gcccgaccag tgagctgtcc ggaagttcgc ccacccgaag cacgcagtcg acgccatcgc gagtcagatc caccagacga tccgcctcgc tgatctgcac ctggatatcc ggataggtgt ccagaaagct cggtaacgct ggcatcatga aatatcgcgc gagtgtgccg tgaacgtcga ctcgcaccag acccgacggc tttgcgccgt tgaatgcggc ttcggcttcc tcgacatccg caatgatgcc aagacaacgc cggtagaaag cctcgccgtc gagcgtcggc cgcacctgcc gcgtcgtccg ctgaagcaac tgcgcgccga ggcgccgctc cagttcctga atcccctgcg tcacgctcga acgcggcacg tccagatccc gcgcggcggc agcaaaactg ccgcgctcca cgatccggac gagcaggcgc atggcgtcga tccgatccat cgaaccagga ccctgggtct gagcttccag ggtanccata tgatng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRIDAMRL LVRIVERGSF AAAARDLDVP RSSVTQGIQE LERRLGAQLL QRTTRQVRPT LDGEAFYRRC LGIIADVEEA EAAFNGAKPS GLVRVDVHGT LARYFMMPAL PSFLDTYPDI QVQISEADRL VDLTRDGVDC VLRVGELPDS SLVGRRIAML KQGTFASPEY LARHGVPQHP AQLEGHRMVG YLSQITGEII PLEFLVAGKR QSMTLPASVM VTGAETNISA ARLGLGLVQV PRYRVADDLS AGTLAEVLGK FPPRPLPVHV LYTRTPQLSP RVRVFVDWLA KEFAARLSD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatcggatc gacgccatgc gcctgctcgt ccggatcgtg gagcgcggca gttttgctgc cgccgcgcgg gatctggacg tgccgcgttc gagcgtgacg caggggattc aggaactgga gcggcgcctc ggcgcgcagt tgcttcagcg gacgacgcgg caggtgcggc cgacgctcga cggcgaggct ttctaccggc gttgtcttgg catcattgcg gatgtcgagg aagccgaagc cgcattcaac ggcgcaaagc cgtcgggtct ggtgcgagtc gacgttcacg gcacactcgc gcgatatttc atgatgccag cgttaccgag ctttctggac acctatccgg atatccaggt gcagatcagc gaggcggatc gtctggtgga tctgactcgc gatggcgtcg actgcgtgct tcgggtgggc gaacttccgg acagctcact ggtcgggcgc cggattgcca tgctgaagca ggggacgttt gccagcccgg aatatctggc gcggcacggc gtgccgcagc atcctgctca acttgagggg caccgcatgg ttggctacct gtcccagatc acgggcgaga ttatcccgct ggaattcctg gtggccggga agcgccagag catgacattg ccggccagcg tcatggtgac gggcgctgaa acgaacatta gcgccgcccg gctgggactg ggtctggtcc aggtgccgcg ctaccgcgtg gccgatgatc tctcggcggg aacgctggcg gaagtgctgg ggaaatttcc cccgcggccg ctgcctgtcc acgtgctgta cacgcgcacg ccccagctct cgccgcgcgt gcgcgtgttc gtggactggc tggcgaagga atttgccgcc agattatccg actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa