BuxeA.12800.s

Transcriptional regulator, LysR family

CENTER ID: BuxeA.12800.s
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.12800.s.A1.GE32105 Full length( BuxeA.12800.s ) 1 317
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.2203
RefSeq: YP_558696.1
UniProt: Q13Z45

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLPAQSTRV SSGERERLDL LDVALFVRAA LLANVSAAGR EFGLSAAVAS SRIAQLEKLL GARLLHRTTR RISLTQDGEV FMTRAEALLD AAAAARASVG RAQAEPQGRL RVSMPSSFGR QHVSPVISEF LRRYPGVSVD LRLTDQVIDL VDAGIDVAIR VGVLKDSSLV ARRLAVNRRV LCAAPAYLAE RGTPHHPSDL AQHECMILSD QRDWAFVTPA GPIEVRVSGR LVTDNGEVIR DALLAGFGIA LKSTWDVAPF LRSGELVSVL DSYPLAERVA IWAVYPSRSF VPPKTLAFID FLAAHFGDPP YWDGEPG
NT Sequence
ATGAATCTTC CTGCGCAATC CACCCGTGTT TCGTCCGGCG AGCGGGAGCG GCTCGATCTG CTCGACGTCG CCCTGTTCGT GCGGGCCGCC CTGCTCGCCA ACGTATCGGC GGCCGGGCGC GAATTCGGCT TGTCGGCGGC GGTGGCCAGT TCGCGCATCG CGCAGCTGGA AAAGCTGCTC GGCGCGCGTT TGCTGCACCG AACCACCCGC CGCATCAGCC TCACGCAGGA CGGTGAAGTC TTCATGACGC GCGCCGAAGC CCTGCTCGAC GCGGCCGCCG CCGCGCGCGC CTCGGTCGGC CGGGCACAGG CCGAGCCGCA GGGCCGCTTG CGCGTTTCGA TGCCCTCGTC GTTCGGGCGG CAGCACGTCT CGCCGGTGAT CAGCGAGTTT CTGCGCCGCT ATCCCGGCGT GAGCGTCGAT CTGCGCCTGA CCGATCAGGT GATCGATCTC GTCGATGCGG GTATCGACGT GGCGATCCGG GTCGGCGTGC TGAAGGATTC GTCGCTGGTG GCGCGGCGCC TCGCGGTGAA CCGGCGGGTG CTGTGCGCGG CGCCCGCTTA TCTTGCCGAA CGCGGCACGC CGCATCATCC TTCGGATCTC GCGCAGCACG AGTGCATGAT CCTGTCGGAC CAGCGCGACT GGGCTTTCGT GACACCCGCC GGGCCGATCG AGGTACGTGT GAGCGGACGC CTCGTCACGG ATAACGGCGA AGTGATCCGC GACGCCCTGC TCGCCGGTTT CGGTATCGCG CTCAAATCGA CGTGGGATGT CGCGCCGTTT CTGCGCAGCG GCGAGCTGGT CAGCGTGCTG GACAGCTACC CGCTCGCCGA ACGGGTGGCA ATCTGGGCGG TGTATCCGAG CCGTTCGTTC GTGCCGCCGA AGACGCTCGC CTTTATCGAC TTCCTCGCCG CCCATTTCGG CGACCCACCT TACTGGGACG GCGAGCCGGG T
Details for BuxeA.12800.s.A1.GE32105
HARVESTED ON: 4/19/2011
SEQUENCED ON: 4/25/2011
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMNLPAQS TRVSSGERER LDLLDVALFV RAALLANVSA AGREFGLSAA VASSRIAQLE KLLGARLLHR TTRRISLTQD GEVFMTRAEA LLDAAAAARA SVGRAQAEPQ GRLRVSMPSS FGRQHVSPVI SEFLRRYPGV SVDLRLTDQV IDLVDAGIDV AIRVGVLKDS SLVARRLAVN RRVLCAAPAY LAERGTPHHP SDLAQHECMI LSDQRDWAFV TPAGPIEVRV SGRLVTDNGE VIRDALLAGF GIALKSTWDV APFLRSGELV SVLDSYPLAE RVAIWAVYPS RSFVPPKTLA FIDFLAAHFG DPPYWDGEP
Validated NT Sequence
acccggctcg ccgtcccagt aaggtgggtc gccgaaatgg gcggcgagga agtcgataaa ggcgagcgtc ttcggcggca cgaacgaacg gctcggatac accgcccaga ttgccacccg ttcggcgagc gggtagctgt ccagcacgct gaccagctcg ccgctgcgca gaaacggcgc gacatcccac gtcgatttga gcgcgatacc gaaaccggcg agcagggcgt cgcggatcac ttcgccgtta tccgtgacga ggcgtccgct cacacgtacc tcgatcggcc cggcgggtgt cacgaaagcc cagtcgcgct ggtccgacag gatcatgcac tcgtgctgcg cgagatccga aggatgatgc ggcgtgccgc gttcggcaag ataagcgggc gccgcgcaca gcacccgccg gttcaccgcg aggcgccgcg ccaccagcga cgaatccttc agcacgccga cccggatcgc cacgtcgata cccgcatcga cgagatcgat cacctgatcg gtcaggcgca gatcgacgct cacgccggga tagcggcgca gaaactcgct gatcaccggc gagacgtgct gccgcccgaa cgacgagggc atcgaaacgc gcaagcggcc ctgcggctcg gcctgtgccc ggccgaccga ggcgcgcgcg gcggcggccg cgtcgagcag ggcttcggcg cgcgtcatga agacttcacc gtcctgcgtg aggctgatgc ggcgggtggt tcggtgcagc aaacgcgcgc cgagcagctt ttccagctgc gcgatgcgcg aactggccac cgccgccgac aagccgaatt cgcgcccggc cgccgatacg ttggcgagca gggcggcccg cacgaacagg gcgacgtcga gcagatcgag ccgctcccgc tcgccggacg aaacacgggt ggattgcgca ggaagattca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNLPAQSTR VSSGERERLD LLDVALFVRA ALLANVSAAG REFGLSAAVA SSRIAQLEKL LGARLLHRTT RRISLTQDGE VFMTRAEALL DAAAAARASV GRAQAEPQGR LRVSMPSSFG RQHVSPVISE FLRRYPGVSV DLRLTDQVID LVDAGIDVAI RVGVLKDSSL VARRLAVNRR VLCAAPAYLA ERGTPHHPSD LAQHECMILS DQRDWAFVTP AGPIEVRVSG RLVTDNGEVI RDALLAGFGI ALKSTWDVAP FLRSGELVSV LDSYPLAERV AIWAVYPSRS FVPPKTLAFI DFLAAHFGDP PYWDGEPG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatcttcct gcgcaatcca cccgtgtttc gtccggcgag cgggagcggc tcgatctgct cgacgtcgcc ctgttcgtgc gggccgccct gctcgccaac gtatcggcgg ccgggcgcga attcggcttg tcggcggcgg tggccagttc gcgcatcgcg cagctggaaa agctgctcgg cgcgcgtttg ctgcaccgaa ccacccgccg catcagcctc acgcaggacg gtgaagtctt catgacgcgc gccgaagccc tgctcgacgc ggccgccgcc gcgcgcgcct cggtcggccg ggcacaggcc gagccgcagg gccgcttgcg cgtttcgatg ccctcgtcgt tcgggcggca gcacgtctcg ccggtgatca gcgagtttct gcgccgctat cccggcgtga gcgtcgatct gcgcctgacc gatcaggtga tcgatctcgt cgatgcgggt atcgacgtgg cgatccgggt cggcgtgctg aaggattcgt cgctggtggc gcggcgcctc gcggtgaacc ggcgggtgct gtgcgcggcg cccgcttatc ttgccgaacg cggcacgccg catcatcctt cggatctcgc gcagcacgag tgcatgatcc tgtcggacca gcgcgactgg gctttcgtga cacccgccgg gccgatcgag gtacgtgtga gcggacgcct cgtcacggat aacggcgaag tgatccgcga cgccctgctc gccggtttcg gtatcgcgct caaatcgacg tgggatgtcg cgccgtttct gcgcagcggc gagctggtca gcgtgctgga cagctacccg ctcgccgaac gggtggcaat ctgggcggtg tatccgagcc gttcgttcgt gccgccgaag acgctcgcct ttatcgactt cctcgccgcc catttcggcg acccacctta ctgggacggc gagccgggtt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa