SSGCID: Target Page

BuxeA.12800.q

Transcriptional regulator, LysR family

CENTER ID:	BuxeA.12800.q
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.12800.q.A1.GE32103	Full length( BuxeA.12800.q )		1	355

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.12800.q.A1.PS01115	Full length( BuxeA.12800.q )		1	355

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.2576
RefSeq:	YP_559047.1
UniProt:	Q13Y44

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNQLQSMRVF VKVADLGSFV GAAGALDLST AVVTRHVADL EARLGTRLLN RTTRRLSLTE SGSTYLERVR HILDDLEGVE QMVVARNHEP VGTLRIVAPV VFGLHSLAPV VQSYAARYPD VVPDVTLADR HVDLVEEGFD VGILVARHMR GASIVTRRLT TGFMTVCATP AYLAQHGTPT RPEHLLAHPC LSRPAEQGGE ERVFAGPDGE VRVRPTNAIV ANNAEMLRQF ALLGMGVAIL PSYLIGRDLA SGRLVPLLSN YRLPQIEITI AYPSRLHLPA KVRTFIDHLV EYFQQPGQQP RDPRMMTPVR SVADKVDRVE MAKGAKSLPT PALSADAARP AHKAARARIA ASSPF

NT Sequence

ATGAACCAAT TGCAGTCGAT GCGCGTATTC GTCAAGGTGG CCGACCTCGG CAGTTTCGTC GGCGCGGCGG GCGCGCTGGA TCTTTCCACC GCGGTCGTTA CGCGCCACGT AGCCGATCTG GAAGCGCGCC TCGGCACGCG GCTGCTCAAT CGCACCACGC GCCGCCTGTC GCTGACCGAA TCCGGCAGCA CGTATCTGGA ACGCGTGCGC CACATTCTCG ACGACCTGGA AGGCGTGGAG CAGATGGTGG TGGCGCGCAA TCACGAGCCG GTCGGCACCT TGCGGATCGT CGCGCCCGTG GTGTTCGGGC TGCATAGTCT CGCACCGGTG GTGCAGTCTT ATGCCGCGCG GTATCCGGAT GTCGTGCCCG ACGTCACGCT CGCGGATCGG CATGTCGATC TGGTGGAAGA GGGCTTCGAT GTCGGCATTC TCGTGGCGCG GCACATGCGC GGCGCGAGCA TCGTCACGCG GCGGTTGACC ACGGGCTTTA TGACGGTTTG CGCGACGCCT GCTTATCTGG CGCAGCATGG CACGCCGACG CGGCCCGAGC ATCTGCTGGC GCATCCGTGC CTGAGCCGGC CGGCCGAGCA GGGCGGCGAG GAGCGCGTGT TCGCCGGGCC GGACGGCGAA GTGCGCGTGC GTCCCACGAA CGCGATCGTG GCGAACAACG CGGAGATGCT CAGGCAATTC GCGCTGCTGG GCATGGGCGT GGCGATTCTG CCGAGCTATC TGATTGGCCG CGATCTGGCG TCCGGGCGGC TCGTGCCGCT GCTTTCCAAC TACCGTCTGC CGCAGATCGA AATCACCATC GCCTATCCGA GCCGCCTGCA TCTGCCGGCG AAGGTGCGCA CTTTCATCGA CCATCTGGTC GAGTATTTCC AGCAGCCGGG TCAGCAACCG CGCGATCCGC GCATGATGAC GCCGGTGCGT TCCGTGGCGG ATAAGGTGGA TAGAGTGGAG ATGGCGAAGG GTGCGAAAAG CCTGCCGACG CCGGCGCTGT CCGCGGACGC GGCGCGCCCC GCGCATAAGG CGGCGCGCGC GCGGATCGCC GCTTCGTCGC CGTTC

Details for BuxeA.12800.q.A1.GE32103

HARVESTED ON:	4/19/2011
SEQUENCED ON:	4/25/2011
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	84
PERCENT COVERAGE:	97

Validated AA Sequence

MXXXXPXXXH XHHHMGTLEA QTQGPGSMNQ LQSMRVFVKV ADLGSFVGAA GALDLSTAVV TRHVADLEAR LGTRLLNRTT RRLSLTESGS TYLERVRHIL DDLEGVEQMV VARNHEPVGT LRIVAPVVFG LHSLAPVVQS YAARYPDVVP DVTLADRHVD LVEEGFDVGI LVARHMRGAS IVTRRLTTGF MTVCATPAYL AQHGTPTRPE HLLAHPCLSR PAEQGGEERV FAGPDGEXRV RPTNAIVANN AEMLRQFALL GMGVAILXSY LIGRDLASGR LVPLLSNYRL PXIEITIAYP SRLHLPAXXX TFIXHLVXXX QXXGQXXXSA XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX

Validated NT Sequence

ccnnnnnnnn nnnngnnnnn nannngcnnn nnnncnatgt nnnnnnnnnt nccttngnnn nnncaccntc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg aaccaattgc agtcgatgcg cgtattcgtc aaggtggccg acctcggcag tttcgtcggc gcggcgggcg cgctggatct ttccaccgcg gtcgttacgc gccacgtagc cgatctggaa gcgcgcctcg gcacgcggct gctcaatcgc accacgcgcc gcctgtcgct gaccgaatcc ggcagcacgt atctggaacg cgtgcgccac attctcgacg acctggaagg cgtggagcag atggtggtgg cgcgcaatca cgagccggtc ggcaccttgc ggatcgtcgc gcccgtggtg ttcgggctgc atagtctcgc accggtggtg cagtcttatg ccgcgcggta tccggatgtc gtgcccgacg tcacgctcgc ggatcggcat gtcgatctgg tggaagaggg cttcgatgtc ggcattctcg tggcgcggca catgcgcggc gcgagcatcg tcacgcggcg gttgaccacg ggctttatga cggtttgcgc gacgcctgct tatctggcgc agcatggcac gccgacgcgg cccgagcatc tgctggcgca tccgtgcctg agccggccgg ccgagcaggg cggcgaggag cgcgtgttcg ccgggccgga cggcgaantg cgcgtgcgtc ccacgaacgc gatcgtggcg aacaacgcgg agatgctcag gcaattcgcg ctgctgggca tgggcgtggc gattctgncg agctatctga ttggccgcga tctggcgtcc gggcggctcg tgccgctgct ttccaactac cgtctgccgc anatcgaaat caccatcgcc tatccnagcc gcctgcatct gccggcgaan gngnncactt tcatcganca tctggtcnag tanttncagc ancnnggnca gnnnnnnnga tccgcnnatg nngnnnnnnn cnnnncnngg nngnnaannn gnnnnnanng nannnggcna nnnnngnnaa nncnngnncn nnncnnnnnn nnnnnnnnnn ncnnnnntna nnnnng

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNQLQSMRV FVKVADLGSF VGAAGALDLS TAVVTRHVAD LEARLGTRLL NRTTRRLSLT ESGSTYLERV RHILDDLEGV EQMVVARNHE PVGTLRIVAP VVFGLHSLAP VVQSYAARYP DVVPDVTLAD RHVDLVEEGF DVGILVARHM RGASIVTRRL TTGFMTVCAT PAYLAQHGTP TRPEHLLAHP CLSRPAEQGG EERVFAGPDG EVRVRPTNAI VANNAEMLRQ FALLGMGVAI LPSYLIGRDL ASGRLVPLLS NYRLPQIEIT IAYPSRLHLP AKVRTFIDHL VEYFQQPGQQ PRDPRMMTPV RSVADKVDRV EMAKGAKSLP TPALSADAAR PAHKAARARI AASSPF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaccaattg cagtcgatgc gcgtattcgt caaggtggcc gacctcggca gtttcgtcgg cgcggcgggc gcgctggatc tttccaccgc ggtcgttacg cgccacgtag ccgatctgga agcgcgcctc ggcacgcggc tgctcaatcg caccacgcgc cgcctgtcgc tgaccgaatc cggcagcacg tatctggaac gcgtgcgcca cattctcgac gacctggaag gcgtggagca gatggtggtg gcgcgcaatc acgagccggt cggcaccttg cggatcgtcg cgcccgtggt gttcgggctg catagtctcg caccggtggt gcagtcttat gccgcgcggt atccggatgt cgtgcccgac gtcacgctcg cggatcggca tgtcgatctg gtggaagagg gcttcgatgt cggcattctc gtggcgcggc acatgcgcgg cgcgagcatc gtcacgcggc ggttgaccac gggctttatg acggtttgcg cgacgcctgc ttatctggcg cagcatggca cgccgacgcg gcccgagcat ctgctggcgc atccgtgcct gagccggccg gccgagcagg gcggcgagga gcgcgtgttc gccgggccgg acggcgaagt gcgcgtgcgt cccacgaacg cgatcgtggc gaacaacgcg gagatgctca ggcaattcgc gctgctgggc atgggcgtgg cgattctgcc gagctatctg attggccgcg atctggcgtc cgggcggctc gtgccgctgc tttccaacta ccgtctgccg cagatcgaaa tcaccatcgc ctatccgagc cgcctgcatc tgccggcgaa ggtgcgcact ttcatcgacc atctggtcga gtatttccag cagccgggtc agcaaccgcg cgatccgcgc atgatgacgc cggtgcgttc cgtggcggat aaggtggata gagtggagat ggcgaagggt gcgaaaagcc tgccgacgcc ggcgctgtcc gcggacgcgg cgcgccccgc gcataaggcg gcgcgcgcgc ggatcgccgc ttcgtcgccg ttctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for BuxeA.12800.q.A1.PS01115

PURIFICATION DATe:	6/1/2011
CONCENTRATION:	11.3mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	100µl
PERCENT IDENTITY:	84
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACCAATTG CAGTCGATGC GCGTATTCGT CAAGGTGGCC GACCTCGGCA GTTTCGTCGG CGCGGCGGGC GCGCTGGATC TTTCCACCGC GGTCGTTACG CGCCACGTAG CCGATCTGGA AGCGCGCCTC GGCACGCGGC TGCTCAATCG CACCACGCGC CGCCTGTCGC TGACCGAATC CGGCAGCACG TATCTGGAAC GCGTGCGCCA CATTCTCGAC GACCTGGAAG GCGTGGAGCA GATGGTGGTG GCGCGCAATC ACGAGCCGGT CGGCACCTTG CGGATCGTCG CGCCCGTGGT GTTCGGGCTG CATAGTCTCG CACCGGTGGT GCAGTCTTAT GCCGCGCGGT ATCCGGATGT CGTGCCCGAC GTCACGCTCG CGGATCGGCA TGTCGATCTG GTGGAAGAGG GCTTCGATGT CGGCATTCTC GTGGCGCGGC ACATGCGCGG CGCGAGCATC GTCACGCGGC GGTTGACCAC GGGCTTTATG ACGGTTTGCG CGACGCCTGC TTATCTGGCG CAGCATGGCA CGCCGACGCG GCCCGAGCAT CTGCTGGCGC ATCCGTGCCT GAGCCGGCCG GCCGAGCAGG GCGGCGAGGA GCGCGTGTTC GCCGGGCCGG ACGGCGAAGT GCGCGTGCGT CCCACGAACG CGATCGTGGC GAACAACGCG GAGATGCTCA GGCAATTCGC GCTGCTGGGC ATGGGCGTGG CGATTCTGCC GAGCTATCTG ATTGGCCGCG ATCTGGCGTC CGGGCGGCTC GTGCCGCTGC TTTCCAACTA CCGTCTGCCG CAGATCGAAA TCACCATCGC CTATCCGAGC CGCCTGCATC TGCCGGCGAA GGTGCGCACT TTCATCGACC ATCTGGTCGA GTATTTCCAG CAGCCGGGTC AGCAACCGCG CGATCCGCGC ATGATGACGC CGGTGCGTTC CGTGGCGGAT AAGGTGGATA GAGTGGAGAT GGCGAAGGGT GCGAAAAGCC TGCCGACGCC GGCGCTGTCC GCGGACGCGG CGCGCCCCGC GCATAAGGCG GCGCGCGCGC GGATCGCCGC TTCGTCGCCG TTCTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA