BuxeA.12800.p

Transcriptional regulator, LysR family

CENTER ID: BuxeA.12800.p
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.12800.p.A1.GE32102 Full length( BuxeA.12800.p ) 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.12800.p.A1.PS01160 Full length( BuxeA.12800.p ) 1 300

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.2903
RefSeq: YP_559358.1
UniProt: Q13X83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRFKQIETF VTVAAKGSLS AAASAEGVAP AIIGRRIDAL EERLGVKLLV RTTRKLTLTF EGSAFLEDCQ RIIHEMQNAE ASVSAGGVKA SGHLRLSAPA GFGRRHVAPL VPAFTVAHPD VSITLDLSDR LVDLVNEGFD CAVRLGELPD SSLVSLRLGE NRRVCVASPA YLSRRGAPHS LADLAHHNCL ALGASANQQR GWMFQQDDKV VSIKVSGTME CSDGAVLHDW CLEGYGLAWR SWWEVGTDIA AGRLVSVLDE FAAPPIGIHA VFPQRRHLPL RVRLFLDFLK HTYGHPGYWG
NT Sequence
ATGGACCGTT TCAAGCAGAT CGAGACTTTC GTGACCGTCG CGGCCAAAGG CAGCCTGTCC GCTGCGGCTT CGGCCGAAGG CGTGGCGCCG GCCATCATCG GGCGACGCAT CGACGCGCTC GAAGAGCGTC TCGGCGTCAA ATTGCTGGTG CGCACCACGC GCAAGCTCAC GCTGACCTTC GAAGGCTCGG CCTTTCTCGA AGACTGTCAG CGCATCATTC ACGAGATGCA GAATGCGGAA GCCAGCGTGT CGGCGGGCGG CGTCAAGGCG AGCGGCCATC TGCGGCTCTC CGCGCCCGCG GGCTTCGGAC GCCGGCACGT CGCGCCACTG GTGCCGGCGT TCACGGTCGC GCATCCGGAC GTGTCGATCA CGCTCGACCT GTCCGACCGG CTCGTCGATC TGGTCAACGA GGGTTTCGAC TGCGCCGTGC GTCTCGGCGA ATTGCCGGAT TCGTCGCTGG TGTCGCTCAG GCTCGGCGAG AACCGCCGGG TTTGCGTCGC GTCGCCCGCG TATCTGAGCC GGCGCGGTGC GCCGCACAGC CTCGCCGATC TCGCGCATCA CAACTGCCTC GCGCTCGGCG CGAGCGCCAA CCAGCAGCGT GGCTGGATGT TCCAGCAGGA CGACAAGGTG GTTTCGATCA AGGTGTCCGG CACGATGGAA TGCTCGGACG GCGCGGTGCT GCACGACTGG TGTCTCGAAG GCTATGGTCT CGCGTGGCGC TCGTGGTGGG AGGTCGGTAC GGACATTGCG GCGGGGCGGC TCGTCAGCGT GCTCGACGAA TTCGCCGCGC CGCCCATCGG CATTCATGCG GTGTTTCCGC AGCGCCGCCA TCTGCCGCTG CGCGTGAGAC TGTTTCTGGA CTTTCTCAAG CATACCTATG GACATCCCGG CTATTGGGGC
Details for BuxeA.12800.p.A1.GE32102
HARVESTED ON: 4/19/2011
SEQUENCED ON: 4/25/2011
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMDRFKQI ETFVTVAAKG SLSAAASAEG VAPAIIGRRI DALEERLGVK LLVRTTRKLT LTFEGSAFLE DCQRIIHEMQ NAEASVSAGG VKASGHLRLS APAGFGRRHV APLVPAFTVA HPDVSITLDL SDRLVDLVNE GFDCAVRLGE LPDSSLVSLR LGENRRVCVA SPAYLSRRGA PHSLADLAHH NCLALGASAN QQRGWMFQQD DKVVSIKVSG TMECSDGAVL HDWCLEGYGL AWRSWWEVGT DIAAGRLVSV LDEFAAPPIG IHAVFPQRRH LPLRVRLFLD FLKHTYGHPG Y
Validated NT Sequence
caatagccgg gatgtccata ggtatgcttg agaaagtcca gaaacagtct cacgcgcagc ggcagatggc ggcgctgcgg aaacaccgca tgaatgccga tgggcggcgc ggcgaattcg tcgagcacgc tgacgagccg ccccgccgca atgtccgtac cgacctccca ccacgagcgc cacgcgagac catagccttc gagacaccag tcgtgcagca ccgcgccgtc cgagcattcc atcgtgccgg acaccttgat cgaaaccacc ttgtcgtcct gctggaacat ccagccacgc tgctggttgg cgctcgcgcc gagcgcgagg cagttgtgat gcgcgagatc ggcgaggctg tgcggcgcac cgcgccggct cagatacgcg ggcgacgcga cgcaaacccg gcggttctcg ccgagcctga gcgacaccag cgacgaatcc ggcaattcgc cgagacgcac ggcgcagtcg aaaccctcgt tgaccagatc gacgagccgg tcggacaggt cgagcgtgat cgacacgtcc ggatgcgcga ccgtgaacgc cggcaccagt ggcgcgacgt gccggcgtcc gaagcccgcg ggcgcggaga gccgcagatg gccgctcgcc ttgacgccgc ccgccgacac gctggcttcc gcattctgca tctcgtgaat gatgcgctga cagtcttcga gaaaggccga gccttcgaag gtcagcgtga gcttgcgcgt ggtgcgcacc agcaatttga cgccgagacg ctcttcgagc gcgtcgatgc gtcgcccgat gatggccggc gccacgcctt cggccgaagc cgcagcggac aggctgcctt tggccgcgac ggtcacgaaa gtctcgatct gcttgaaacg gtccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat gg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRFKQIET FVTVAAKGSL SAAASAEGVA PAIIGRRIDA LEERLGVKLL VRTTRKLTLT FEGSAFLEDC QRIIHEMQNA EASVSAGGVK ASGHLRLSAP AGFGRRHVAP LVPAFTVAHP DVSITLDLSD RLVDLVNEGF DCAVRLGELP DSSLVSLRLG ENRRVCVASP AYLSRRGAPH SLADLAHHNC LALGASANQQ RGWMFQQDDK VVSIKVSGTM ECSDGAVLHD WCLEGYGLAW RSWWEVGTDI AAGRLVSVLD EFAAPPIGIH AVFPQRRHLP LRVRLFLDFL KHTYGHPGYW G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccgtttc aagcagatcg agactttcgt gaccgtcgcg gccaaaggca gcctgtccgc tgcggcttcg gccgaaggcg tggcgccggc catcatcggg cgacgcatcg acgcgctcga agagcgtctc ggcgtcaaat tgctggtgcg caccacgcgc aagctcacgc tgaccttcga aggctcggcc tttctcgaag actgtcagcg catcattcac gagatgcaga atgcggaagc cagcgtgtcg gcgggcggcg tcaaggcgag cggccatctg cggctctccg cgcccgcggg cttcggacgc cggcacgtcg cgccactggt gccggcgttc acggtcgcgc atccggacgt gtcgatcacg ctcgacctgt ccgaccggct cgtcgatctg gtcaacgagg gtttcgactg cgccgtgcgt ctcggcgaat tgccggattc gtcgctggtg tcgctcaggc tcggcgagaa ccgccgggtt tgcgtcgcgt cgcccgcgta tctgagccgg cgcggtgcgc cgcacagcct cgccgatctc gcgcatcaca actgcctcgc gctcggcgcg agcgccaacc agcagcgtgg ctggatgttc cagcaggacg acaaggtggt ttcgatcaag gtgtccggca cgatggaatg ctcggacggc gcggtgctgc acgactggtg tctcgaaggc tatggtctcg cgtggcgctc gtggtgggag gtcggtacgg acattgcggc ggggcggctc gtcagcgtgc tcgacgaatt cgccgcgccg cccatcggca ttcatgcggt gtttccgcag cgccgccatc tgccgctgcg cgtgagactg tttctggact ttctcaagca tacctatgga catcccggct attggggcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BuxeA.12800.p.A1.PS01160
PURIFICATION DATe: 7/19/2011
CONCENTRATION: 3.3mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMDRFKQI ETFVTVAAKG SLSAAASAEG VAPAIIGRRI DALEERLGVK LLVRTTRKLT LTFEGSAFLE DCQRIIHEMQ NAEASVSAGG VKASGHLRLS APAGFGRRHV APLVPAFTVA HPDVSITLDL SDRLVDLVNE GFDCAVRLGE LPDSSLVSLR LGENRRVCVA SPAYLSRRGA PHSLADLAHH NCLALGASAN QQRGWMFQQD DKVVSIKVSG TMECSDGAVL HDWCLEGYGL AWRSWWEVGT DIAAGRLVSV LDEFAAPPIG IHAVFPQRRH LPLRVRLFLD FLKHTYGHPG Y
Validated NT Sequence
caatagccgg gatgtccata ggtatgcttg agaaagtcca gaaacagtct cacgcgcagc ggcagatggc ggcgctgcgg aaacaccgca tgaatgccga tgggcggcgc ggcgaattcg tcgagcacgc tgacgagccg ccccgccgca atgtccgtac cgacctccca ccacgagcgc cacgcgagac catagccttc gagacaccag tcgtgcagca ccgcgccgtc cgagcattcc atcgtgccgg acaccttgat cgaaaccacc ttgtcgtcct gctggaacat ccagccacgc tgctggttgg cgctcgcgcc gagcgcgagg cagttgtgat gcgcgagatc ggcgaggctg tgcggcgcac cgcgccggct cagatacgcg ggcgacgcga cgcaaacccg gcggttctcg ccgagcctga gcgacaccag cgacgaatcc ggcaattcgc cgagacgcac ggcgcagtcg aaaccctcgt tgaccagatc gacgagccgg tcggacaggt cgagcgtgat cgacacgtcc ggatgcgcga ccgtgaacgc cggcaccagt ggcgcgacgt gccggcgtcc gaagcccgcg ggcgcggaga gccgcagatg gccgctcgcc ttgacgccgc ccgccgacac gctggcttcc gcattctgca tctcgtgaat gatgcgctga cagtcttcga gaaaggccga gccttcgaag gtcagcgtga gcttgcgcgt ggtgcgcacc agcaatttga cgccgagacg ctcttcgagc gcgtcgatgc gtcgcccgat gatggccggc gccacgcctt cggccgaagc cgcagcggac aggctgcctt tggccgcgac ggtcacgaaa gtctcgatct gcttgaaacg gtccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat gg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRFKQIET FVTVAAKGSL SAAASAEGVA PAIIGRRIDA LEERLGVKLL VRTTRKLTLT FEGSAFLEDC QRIIHEMQNA EASVSAGGVK ASGHLRLSAP AGFGRRHVAP LVPAFTVAHP DVSITLDLSD RLVDLVNEGF DCAVRLGELP DSSLVSLRLG ENRRVCVASP AYLSRRGAPH SLADLAHHNC LALGASANQQ RGWMFQQDDK VVSIKVSGTM ECSDGAVLHD WCLEGYGLAW RSWWEVGTDI AAGRLVSVLD EFAAPPIGIH AVFPQRRHLP LRVRLFLDFL KHTYGHPGYW G
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACCGTTTC AAGCAGATCG AGACTTTCGT GACCGTCGCG GCCAAAGGCA GCCTGTCCGC TGCGGCTTCG GCCGAAGGCG TGGCGCCGGC CATCATCGGG CGACGCATCG ACGCGCTCGA AGAGCGTCTC GGCGTCAAAT TGCTGGTGCG CACCACGCGC AAGCTCACGC TGACCTTCGA AGGCTCGGCC TTTCTCGAAG ACTGTCAGCG CATCATTCAC GAGATGCAGA ATGCGGAAGC CAGCGTGTCG GCGGGCGGCG TCAAGGCGAG CGGCCATCTG CGGCTCTCCG CGCCCGCGGG CTTCGGACGC CGGCACGTCG CGCCACTGGT GCCGGCGTTC ACGGTCGCGC ATCCGGACGT GTCGATCACG CTCGACCTGT CCGACCGGCT CGTCGATCTG GTCAACGAGG GTTTCGACTG CGCCGTGCGT CTCGGCGAAT TGCCGGATTC GTCGCTGGTG TCGCTCAGGC TCGGCGAGAA CCGCCGGGTT TGCGTCGCGT CGCCCGCGTA TCTGAGCCGG CGCGGTGCGC CGCACAGCCT CGCCGATCTC GCGCATCACA ACTGCCTCGC GCTCGGCGCG AGCGCCAACC AGCAGCGTGG CTGGATGTTC CAGCAGGACG ACAAGGTGGT TTCGATCAAG GTGTCCGGCA CGATGGAATG CTCGGACGGC GCGGTGCTGC ACGACTGGTG TCTCGAAGGC TATGGTCTCG CGTGGCGCTC GTGGTGGGAG GTCGGTACGG ACATTGCGGC GGGGCGGCTC GTCAGCGTGC TCGACGAATT CGCCGCGCCG CCCATCGGCA TTCATGCGGT GTTTCCGCAG CGCCGCCATC TGCCGCTGCG CGTGAGACTG TTTCTGGACT TTCTCAAGCA TACCTATGGA CATCCCGGCT ATTGGGGCTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A