SSGCID: Target Page

BuxeA.12800.o

Transcriptional regulator, LysR family

CENTER ID:	BuxeA.12800.o
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.12800.o.A1.GE32101	Full length( BuxeA.12800.o )		1	312

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.3224
RefSeq:	YP_559658.1
UniProt:	Q13WD3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDTLQNMRVF VRVVEAGSFT GAAQHLNTTT AYASRAVSDL EAHLRTRLLN RTTRRIALTE AGERYLQRCE QILAYVDQAE AEAGDAHARP SGKLKVHAMT SFGQHYVVPA AGRYQQRYPD VHIELTLAQR MPDLLDEGFD VSLTLATGLP DSGLVSQRLG SAFSIACASP AYLELHGVPQ TPADLARHTC LQMVTPVFPT DKWTFDGPNG EETIALGPTT FQVNVAEAMA VAVSSGMGVG LIPIYSAISG LRSGELVWLL PEYTSQEMNL YALYPSRQYL DAKIRTWIEF LRDVLPATLA ADQAELREFA RA

NT Sequence

ATGGATACGC TTCAAAACAT GCGCGTATTT GTCCGTGTCG TCGAAGCGGG CAGCTTTACG GGTGCCGCGC AGCATCTGAA CACGACCACG GCCTATGCGT CGCGCGCGGT GTCCGATCTG GAAGCGCATC TGCGCACCCG ACTGCTGAAC CGCACCACGC GCCGCATTGC GCTGACCGAG GCCGGCGAGC GCTATCTGCA GCGCTGCGAG CAGATTCTCG CGTACGTGGA CCAGGCGGAA GCCGAGGCGG GCGACGCCCA TGCCCGTCCG TCCGGCAAGC TCAAGGTCCA CGCCATGACG AGCTTCGGCC AGCACTATGT GGTGCCCGCT GCTGGCCGCT ATCAGCAGCG CTACCCGGAC GTCCATATCG AACTGACGCT CGCGCAGCGC ATGCCCGATC TGCTCGACGA AGGCTTCGAC GTGTCGCTCA CGCTCGCAAC CGGTTTGCCG GATTCGGGGC TGGTGTCGCA GCGCCTCGGC AGCGCGTTCA GCATTGCATG CGCGTCGCCG GCCTATCTGG AGCTGCACGG CGTGCCGCAA ACGCCCGCGG ACCTTGCGCG TCACACCTGT CTGCAAATGG TCACGCCGGT TTTCCCGACC GACAAATGGA CCTTCGACGG CCCGAACGGC GAGGAAACGA TCGCCCTCGG ACCGACCACT TTCCAGGTGA ACGTGGCCGA AGCGATGGCG GTGGCCGTAT CGAGCGGCAT GGGCGTCGGG CTGATTCCGA TCTACTCGGC GATCAGCGGC TTGCGCAGCG GAGAACTGGT GTGGCTGCTG CCGGAATACA CGTCGCAGGA GATGAATCTG TACGCGTTGT ACCCGTCGCG GCAGTATCTG GACGCGAAGA TTCGCACGTG GATCGAGTTC CTGCGCGACG TATTGCCCGC CACGCTGGCC GCGGATCAGG CGGAGTTGCG CGAGTTCGCG CGCGCC

Details for BuxeA.12800.o.A1.GE32101

HARVESTED ON:	4/19/2011
SEQUENCED ON:	4/25/2011
EXPECTED MW:	36kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

gngcgcgcga actcgcgcaa ctccgcctga tccgcggcca gcgtggcggg caatacgtcg cgcaggaact cgatccacgt gcgaatcttc gcgtccagat actgccgcga cgggtacaac gcgtacagat tcatctcctg cgacgtgtat tccggcagca gccacaccag ttctccgctg cgcaagccgc tgatcgccga gtagatcgga atcagcccga cgcccatgcc gctcgatacg gccaccgcca tcgcttcggc cacgttcacc tggaaagtgg tcggtccgag ggcgatcgtt tcctcgccgt tcgggccgtc gaaggtccat ttgtcggtcg ggaaaaccgg cgtgaccatt tgcagacagg tgtgacgcgc aaggtccgcg ggcgtttgcg gcacgccgtg cagctccaga taggccggcg acgcgcatgc aatgctgaac gcgctgccga ggcgctgcga caccagcccc gaatccggca aaccggttgc gagcgtgagc gacacgtcga agccttcgtc gagcagatcg ggcatgcgct gcgcgagcgt cagttcgata tggacgtccg ggtagcgctg ctgatagcgg ccagcagcgg gcaccacata gtgctggccg aagctcgtca tggcgtggac cttgagcttg ccggacggac gggcatgggc gtcgcccgcc tcggcttccg cctggtccac gtacgcgaga atctgctcgc agcgctgcag atagcgctcg ccggcctcgg tcagcgcaat gcggcgcgtg gtgcggttca gcagtcgggt gcgcagatgc gcttccagat cggacaccgc gcgcgacgca taggccgtgg tcgtgttcag atgctgcgcg gcacccgtaa agctgcccgc ttcgacgaca cggacaaata cgcgcatgtt ttgaagcgta tccatcgaac caggaccctg ggtctgagct tccagggtac c

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDTLQNMRV FVRVVEAGSF TGAAQHLNTT TAYASRAVSD LEAHLRTRLL NRTTRRIALT EAGERYLQRC EQILAYVDQA EAEAGDAHAR PSGKLKVHAM TSFGQHYVVP AAGRYQQRYP DVHIELTLAQ RMPDLLDEGF DVSLTLATGL PDSGLVSQRL GSAFSIACAS PAYLELHGVP QTPADLARHT CLQMVTPVFP TDKWTFDGPN GEETIALGPT TFQVNVAEAM AVAVSSGMGV GLIPIYSAIS GLRSGELVWL LPEYTSQEMN LYALYPSRQY LDAKIRTWIE FLRDVLPATL AADQAELREF ARA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatacgctt caaaacatgc gcgtatttgt ccgtgtcgtc gaagcgggca gctttacggg tgccgcgcag catctgaaca cgaccacggc ctatgcgtcg cgcgcggtgt ccgatctgga agcgcatctg cgcacccgac tgctgaaccg caccacgcgc cgcattgcgc tgaccgaggc cggcgagcgc tatctgcagc gctgcgagca gattctcgcg tacgtggacc aggcggaagc cgaggcgggc gacgcccatg cccgtccgtc cggcaagctc aaggtccacg ccatgacgag cttcggccag cactatgtgg tgcccgctgc tggccgctat cagcagcgct acccggacgt ccatatcgaa ctgacgctcg cgcagcgcat gcccgatctg ctcgacgaag gcttcgacgt gtcgctcacg ctcgcaaccg gtttgccgga ttcggggctg gtgtcgcagc gcctcggcag cgcgttcagc attgcatgcg cgtcgccggc ctatctggag ctgcacggcg tgccgcaaac gcccgcggac cttgcgcgtc acacctgtct gcaaatggtc acgccggttt tcccgaccga caaatggacc ttcgacggcc cgaacggcga ggaaacgatc gccctcggac cgaccacttt ccaggtgaac gtggccgaag cgatggcggt ggccgtatcg agcggcatgg gcgtcgggct gattccgatc tactcggcga tcagcggctt gcgcagcgga gaactggtgt ggctgctgcc ggaatacacg tcgcaggaga tgaatctgta cgcgttgtac ccgtcgcggc agtatctgga cgcgaagatt cgcacgtgga tcgagttcct gcgcgacgta ttgcccgcca cgctggccgc ggatcaggcg gagttgcgcg agttcgcgcg cgcctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa