SSGCID: Target Page

BuxeA.12800.n

Transcriptional regulator, LysR family

CENTER ID:	BuxeA.12800.n
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.12800.n.A2.GE32100	Mature protein( BuxeA.12800.n )		22	313

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.5005
RefSeq:	YP_552648.1
UniProt:	Q13RJ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRLTSLGVF VAAVEEGSFA GAARRFGLSA AMAGKHVSAL EAELNARLLH RTTRRLSLTD TGQTYYERSK RILEAFDEAK REASDSQGTA RGVLRIAAPV TFGAMHLGEV VARYMEDHPH VNVEVLLGDR YVDLIDAGVD VAIRIGKLED PGLVTRRLAP CRMVVCASPA YLKRHGTPRK PDDLLRAQRL AFSEAVSAGD WTLHDRKNRA HVIDGPCRMT ANNTQMLLAS AIAGAGIVYG PTFVFGAHIR SGELVALLPA YRAADLTIQA VYPSARRIPL KVRRFVDYLA ETFGDEPSWD RKEMPQKSGC SGC

NT Sequence

ATGGATCGGC TCACGAGCCT CGGAGTCTTC GTCGCCGCTG TCGAGGAAGG CAGCTTTGCC GGGGCGGCGC GCCGCTTCGG GCTATCGGCG GCGATGGCCG GCAAGCACGT GAGCGCCCTC GAGGCCGAAC TGAATGCCCG GCTACTCCAC CGCACCACAC GGCGTTTGAG TCTGACCGAT ACCGGCCAGA CATACTACGA ACGCAGCAAG CGGATTCTCG AAGCCTTCGA CGAAGCGAAG CGGGAAGCGA GCGATTCGCA AGGCACGGCG CGCGGTGTGC TGCGCATCGC GGCGCCGGTC ACGTTCGGCG CAATGCACCT GGGCGAGGTT GTGGCGCGCT ACATGGAGGA CCATCCGCAT GTCAATGTGG AAGTGCTGCT CGGAGACCGC TACGTCGATC TCATCGATGC CGGCGTTGAC GTCGCCATCC GGATCGGAAA ACTGGAGGAT CCGGGACTTG TGACGCGCCG GCTTGCGCCG TGCCGGATGG TCGTGTGTGC GTCTCCCGCC TACCTCAAGC GCCACGGAAC GCCGCGCAAG CCGGACGACT TGCTGCGTGC GCAGCGACTT GCGTTCAGCG AGGCCGTGTC GGCCGGAGAC TGGACGTTGC ACGACAGGAA AAACCGCGCG CACGTCATCG ACGGACCTTG CCGGATGACG GCCAACAACA CCCAGATGCT GCTCGCCTCC GCTATTGCCG GAGCGGGCAT CGTGTACGGT CCGACCTTCG TGTTCGGCGC GCATATCCGG AGCGGCGAAC TCGTAGCGCT GCTGCCGGCC TATCGCGCGG CTGATCTGAC GATCCAGGCG GTTTATCCGA GCGCGCGGCG CATACCGCTC AAAGTGCGGC GGTTTGTCGA TTACCTCGCC GAGACATTCG GCGATGAACC GTCTTGGGAT CGCAAGGAAA TGCCCCAGAA GAGCGGCTGT TCGGGCTGC

Details for BuxeA.12800.n.A2.GE32100

HARVESTED ON:	4/19/2011
SEQUENCED ON:	4/25/2011
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAARRFGLS AAMAGKHVSA LEAELNARLL HRTTRRLSLT DTGQTYYERS KRILEAFDEA KREASDSQGT ARGVLRIAAP VTFGAMHLGE VVARYMEDHP HVNVEVLLGD RYVDLIDAGV DVAIRIGKLE DPGLVTRRLA PCRMVVCASP AYLKRHGTPR KPDDLLRAQR LAFSEAVSAG DWTLHDRKNR AHVIDGPCRM TANNTQMLLA SAIAGAGIVY GPTFVFGAHI RSGELVALLP AYRAADLTIQ AVYPSARRIP LKVRRFVDYL AETFGDEPSW DRKEMPQKSG CSGC

Validated NT Sequence

ttattagcag cccgaacagc cgctcttctg gggcatttcc ttgcgatccc aagacggttc atcgccgaat gtctcggcga ggtaatcgac aaaccgccgc actttgagcg gtatgcgccg cgcgctcgga taaaccgcct ggatcgtcag atcagccgcg cgataggccg gcagcagcgc tacgagttcg ccgctccgga tatgcgcgcc gaacacgaag gtcggaccgt acacgatgcc cgctccggca atagcggagg cgagcagcat ctgggtgttg ttggccgtca tccggcaagg tccgtcgatg acgtgcgcgc ggtttttcct gtcgtgcaac gtccagtctc cggccgacac ggcctcgctg aacgcaagtc gctgcgcacg cagcaagtcg tccggcttgc gcggcgttcc gtggcgcttg aggtaggcgg gagacgcaca cacgaccatc cggcacggcg caagccggcg cgtcacaagt cccggatcct ccagttttcc gatccggatg gcgacgtcaa cgccggcatc gatgagatcg acgtagcggt ctccgagcag cacttccaca ttgacatgcg gatggtcctc catgtagcgc gccacaacct cgcccaggtg cattgcgccg aacgtgaccg gcgccgcgat gcgcagcaca ccgcgcgccg tgccttgcga atcgctcgct tcccgcttcg cttcgtcgaa ggcttcgaga atccgcttgc tgcgttcgta gtatgtctgg ccggtatcgg tcagactcaa acgccgtgtg gtgcggtgga gtagccgggc attcagttcg gcctcgaggg cgctcacgtg cttgccggcc atcgccgccg atagcccgaa gcggcgcgcc gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccatgg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggcgcgc cgcttcgggc tatcggcggc gatggccggc aagcacgtga gcgccctcga ggccgaactg aatgcccggc tactccaccg caccacacgg cgtttgagtc tgaccgatac cggccagaca tactacgaac gcagcaagcg gattctcgaa gccttcgacg aagcgaagcg ggaagcgagc gattcgcaag gcacggcgcg cggtgtgctg cgcatcgcgg cgccggtcac gttcggcgca atgcacctgg gcgaggttgt ggcgcgctac atggaggacc atccgcatgt caatgtggaa gtgctgctcg gagaccgcta cgtcgatctc atcgatgccg gcgttgacgt cgccatccgg atcggaaaac tggaggatcc gggacttgtg acgcgccggc ttgcgccgtg ccggatggtc gtgtgtgcgt ctcccgccta cctcaagcgc cacggaacgc cgcgcaagcc ggacgacttg ctgcgtgcgc agcgacttgc gttcagcgag gccgtgtcgg ccggagactg gacgttgcac gacaggaaaa accgcgcgca cgtcatcgac ggaccttgcc ggatgacggc caacaacacc cagatgctgc tcgcctccgc tattgccgga gcgggcatcg tgtacggtcc gaccttcgtg ttcggcgcgc atatccggag cggcgaactc gtagcgctgc tgccggccta tcgcgcggct gatctgacga tccaggcggt ttatccgagc gcgcggcgca taccgctcaa agtgcggcgg tttgtcgatt acctcgccga gacattcggc gatgaaccgt cttgggatcg caaggaaatg ccccagaaga gcggctgttc gggctgctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa