SSGCID: Target Page

BuxeA.12800.m

Transcriptional regulator, LysR family

CENTER ID:	BuxeA.12800.m
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.12800.m.A2.GE32099	Mature protein( BuxeA.12800.m )		25	295

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.5029
RefSeq:	YP_552672.1
UniProt:	Q13RG7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRLTSMAIF VKAAESGSFA AAALAFGISS QMAGRHVIAL EERVGAPLLA RTTRRQSLTE IGQIFYERCK ALLADAEAAE SVAMDFSASP RGRLRVTAPV TFGSCCLAPL ITRYLRANPE VQVDLTLTDR FVDLVDEGYE VAIRLGPLSD SSMIAKPLMP YRLLACASPA YLKEHGEPRT PEALARHECL GFSFTSLPPY NEWVFGSARG KHAVTVTGRF QANDTKALIA AAIDGFGIAL APEIAIKDDI AAGRLVRLLR KYEAPERPMH ALFTARRPTP KLRAFIDLVV AEFGP

NT Sequence

ATGGACCGCC TGACCAGCAT GGCTATCTTC GTGAAAGCCG CCGAAAGCGG CTCGTTCGCG GCGGCGGCTC TCGCCTTTGG CATTTCGTCG CAAATGGCTG GACGACATGT GATTGCGCTC GAAGAACGGG TCGGCGCGCC GCTCCTCGCC CGCACGACCC GCCGCCAGAG TCTGACCGAA ATCGGCCAGA TCTTCTACGA ACGCTGCAAG GCCCTGCTCG CGGACGCCGA GGCGGCGGAA TCGGTAGCGA TGGATTTCTC CGCGTCGCCG CGCGGACGGC TACGTGTAAC CGCCCCGGTC ACATTTGGAT CCTGTTGCCT TGCGCCGCTG ATCACGCGCT ACCTTCGCGC GAACCCCGAG GTCCAGGTTG ATCTCACGTT GACGGACCGG TTTGTGGACC TCGTCGATGA AGGTTATGAA GTGGCAATCC GGCTCGGCCC TCTGTCGGAT TCGTCGATGA TCGCAAAACC GCTGATGCCG TATCGACTGC TCGCCTGTGC GTCGCCGGCT TATCTGAAGG AACATGGCGA ACCCAGAACG CCGGAAGCGC TCGCAAGGCA TGAATGCCTG GGGTTTTCCT TCACGTCGCT CCCGCCGTAC AACGAGTGGG TGTTCGGCAG CGCGCGCGGC AAACATGCCG TGACGGTCAC CGGGCGCTTT CAGGCGAACG ATACGAAGGC GCTGATTGCC GCCGCGATTG ACGGCTTCGG CATTGCTCTC GCACCGGAAA TCGCAATCAA GGACGACATT GCCGCGGGCC GGCTCGTGCG CCTTCTGCGC AAGTACGAGG CGCCTGAACG GCCAATGCAC GCGCTGTTTA CGGCGAGGCG GCCCACGCCG AAATTGCGCG CGTTCATTGA TCTGGTGGTC GCGGAATTCG GGCCG

Details for BuxeA.12800.m.A2.GE32099

HARVESTED ON:	4/19/2011
SEQUENCED ON:	4/25/2011
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	109

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAFGISSQM AGRHVIALEE RVGAPLLART TRRQSLTEIG QIFYERCKAL LADAEAAESV AMDFSASPRG RLRVTAPVTF GSCCLAPLIT RYLRANPEVQ VDLTLTDRFV DLVDEGYEVA IRLGPLSDSS MIAKPLMPYR LLACASPAYL KEHGEPRTPE ALARHECLGF SFTSLPPYNE WVFGSARGKH AVTVTGRFQA NDTKALIAAA IDGFGIALAP EIAIKDDIAA GRLVRLLRKY EAPERXMHAL FTARRPTPKL RAFIDLVVAX XXAVXTARTS SAAXLXXXXL LXKXXXXXXX X

Validated NT Sequence

ganggagnna taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gcctttggca tttcgtcgca aatggctgga cgacatgtga ttgcgctcga agaacgggtc ggcgcgccgc tcctcgcccg cacgacccgc cgccagagtc tgaccgaaat cggccagatc ttctacgaac gctgcaaggc cctgctcgcg gacgccgagg cggcggaatc ggtagcgatg gatttctccg cgtcgccgcg cggacggcta cgtgtaaccg ccccggtcac atttggatcc tgttgccttg cgccgctgat cacgcgctac cttcgcgcga accccgaggt ccaggttgat ctcacgttga cggaccggtt tgtggacctc gtcgatgaag gttatgaagt ggcaatccgg ctcggccctc tgtcggattc gtcgatgatc gcaaaaccgc tgatgccgta tcgactgctc gcctgtgcgt cgccggctta tctgaaggaa catggcgaac ccagaacgcc ggaagcgctc gcaaggcatg aatgcctggg gttttccttc acgtcgctcc cgccgtacaa cgagtgggtg ttcggcagcg cgcgcggcaa acatgccgtg acggtcaccg ggcgctttca ggcgaacgat acgaaggcgc tgattgccgc cgcgattgac ggcttcggca ttgctctcgc accggaaatc gcaatcaagg acgacattgc cgcgggccgg ctcgtgcgcc ttctgcgcaa gtacgaggcg cctgaacggn caatgcacgc gctgtttacn gcgaggcggc ccacgccgaa attgcgcgcg ttcattgatc tggtggtcgc gnnnntcngg gccgtannaa cagcacgnac aagttctgca gccnngctnn cgannatncn gctgctanna aagnanngaa gntnantnng ntgntncnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcctttggc atttcgtcgc aaatggctgg acgacatgtg attgcgctcg aagaacgggt cggcgcgccg ctcctcgccc gcacgacccg ccgccagagt ctgaccgaaa tcggccagat cttctacgaa cgctgcaagg ccctgctcgc ggacgccgag gcggcggaat cggtagcgat ggatttctcc gcgtcgccgc gcggacggct acgtgtaacc gccccggtca catttggatc ctgttgcctt gcgccgctga tcacgcgcta ccttcgcgcg aaccccgagg tccaggttga tctcacgttg acggaccggt ttgtggacct cgtcgatgaa ggttatgaag tggcaatccg gctcggccct ctgtcggatt cgtcgatgat cgcaaaaccg ctgatgccgt atcgactgct cgcctgtgcg tcgccggctt atctgaagga acatggcgaa cccagaacgc cggaagcgct cgcaaggcat gaatgcctgg ggttttcctt cacgtcgctc ccgccgtaca acgagtgggt gttcggcagc gcgcgcggca aacatgccgt gacggtcacc gggcgctttc aggcgaacga tacgaaggcg ctgattgccg ccgcgattga cggcttcggc attgctctcg caccggaaat cgcaatcaag gacgacattg ccgcgggccg gctcgtgcgc cttctgcgca agtacgaggc gcctgaacgg ccaatgcacg cgctgtttac ggcgaggcgg cccacgccga aattgcgcgc gttcattgat ctggtggtcg cggaattcgg gccgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa