SSGCID: Target Page

BuxeA.12800.k

Transcriptional regulator, LysR family

CENTER ID:	BuxeA.12800.k
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.12800.k.A1.GE32097	Full length( BuxeA.12800.k )		1	331

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.5875
RefSeq:	YP_553454.1
UniProt:	Q13P85

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDTLQNMRVF VRVVEAGSFT GAAQSLNSTT GAMSRAVSEL EAHLRTRLLN RSTRRLALTT AGERYLTRCQ QILADVSTAE EEASCAHERP TGALRMHSFA SIGQHYVLPA ISRYRALYPE VTVELTLSQR MPDLFEGSAD VAVIGASTLP NSDLVSLPLG TTFSILCASP AYVRAHGAPQ KPADLAHHEC LILHTPAFPP HDWVLEGPNG CETMEVNGPV HVNIAESLIV AIREGMGIGM LPLYAAISGL RDGTLVRVLP QYTAQKMNIY ALYPSRKFID AKTRTWVEFL RTHLPKVIAR DEALLAEVDK AQISDGVIPA GASSGADIAT Q

NT Sequence

ATGGATACGT TACAAAATAT GCGAGTGTTC GTGCGCGTGG TCGAAGCCGG GAGTTTCACC GGTGCCGCGC AGTCGCTCAA TTCAACGACC GGCGCAATGT CCCGCGCGGT TTCGGAACTC GAAGCTCATC TGCGGACCCG GTTGCTGAAC CGCTCCACGC GACGGCTAGC GCTGACCACT GCGGGCGAGC GTTACCTGAC ACGATGTCAG CAGATACTTG CAGATGTCAG CACAGCGGAA GAAGAAGCCA GTTGTGCGCA TGAGCGCCCG ACCGGCGCTT TGAGAATGCA TAGCTTTGCC AGCATCGGCC AGCATTACGT GCTGCCGGCT ATCTCGCGCT ATCGCGCGCT GTATCCGGAG GTGACTGTCG AGCTGACGTT GTCGCAGCGC ATGCCTGATC TCTTTGAAGG CAGCGCGGAT GTCGCGGTGA TCGGCGCTTC CACTTTGCCT AATTCGGATC TCGTGTCGCT TCCGCTTGGA ACGACCTTCA GCATCTTGTG CGCTTCGCCG GCGTATGTGC GCGCCCACGG CGCGCCGCAG AAGCCGGCCG ATCTGGCACA CCACGAATGT CTGATCCTGC ATACCCCGGC GTTTCCGCCG CACGACTGGG TGCTCGAAGG GCCGAATGGC TGCGAAACGA TGGAGGTGAA TGGGCCTGTG CACGTGAACA TCGCCGAGTC GCTGATCGTC GCGATTCGCG AAGGAATGGG CATTGGCATG CTGCCGCTCT ACGCGGCAAT TTCCGGATTA CGTGATGGAA CGTTAGTGCG CGTGTTGCCG CAATATACCG CGCAGAAGAT GAATATTTAC GCGCTATATC CGTCGCGTAA GTTCATCGAT GCGAAGACCC GGACTTGGGT CGAATTCTTG CGTACGCACC TGCCCAAAGT CATTGCCCGC GACGAAGCAT TGCTCGCAGA AGTCGACAAA GCGCAGATTA GCGATGGCGT GATACCCGCT GGCGCAAGCA GCGGCGCGGA TATCGCGACG CAG

Details for BuxeA.12800.k.A1.GE32097

HARVESTED ON:	4/19/2011
SEQUENCED ON:	4/25/2011
EXPECTED MW:	38kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

ttattactgc gtcgcgatat ccgcgccgct gcttgcgcca gcgggtatca cgccatcgct aatctgcgct ttgtcgactt ctgcgagcaa tgcttcgtcg cgggcaatga ctttgggcag gtgcgtacgc aagaattcga cccaagtccg ggtcttcgca tcgatgaact tacgcgacgg atatagcgcg taaatattca tcttctgcgc ggtatattgc ggcaacacgc gcactaacgt tccatcacgt aatccggaaa ttgccgcgta gagcggcagc atgccaatgc ccattccttc gcgaatcgcg acgatcagcg actcggcgat gttcacgtgc acaggcccat tcacctccat cgtttcgcag ccattcggcc cttcgagcac ccagtcgtgc ggcggaaacg ccggggtatg caggatcaga cattcgtggt gtgccagatc ggccggcttc tgcggcgcgc cgtgggcgcg cacatacgcc ggcgaagcgc acaagatgct gaaggtcgtt ccaagcggaa gcgacacgag atccgaatta ggcaaagtgg aagcgccgat caccgcgaca tccgcgctgc cttcaaagag atcaggcatg cgctgcgaca acgtcagctc gacagtcacc tccggataca gcgcgcgata gcgcgagata gccggcagca cgtaatgctg gccgatgctg gcaaagctat gcattctcaa agcgccggtc gggcgctcat gcgcacaact ggcttcttct tccgctgtgc tgacatctgc aagtatctgc tgacatcgtg tcaggtaacg ctcgcccgca gtggtcagcg ctagccgtcg cgtggagcgg ttcagcaacc gggtccgcag atgagcttcg agttccgaaa ccgcgcggga cattgcgccg gtcgttgaat tgagcgactg cgcggcaccg gtgaaactcc cggcttcgac cacgcgcacg aacactcgca tattttgtaa cgtatccatc gaaccaggac cctgggtctg agcttccagg gtacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDTLQNMRV FVRVVEAGSF TGAAQSLNST TGAMSRAVSE LEAHLRTRLL NRSTRRLALT TAGERYLTRC QQILADVSTA EEEASCAHER PTGALRMHSF ASIGQHYVLP AISRYRALYP EVTVELTLSQ RMPDLFEGSA DVAVIGASTL PNSDLVSLPL GTTFSILCAS PAYVRAHGAP QKPADLAHHE CLILHTPAFP PHDWVLEGPN GCETMEVNGP VHVNIAESLI VAIREGMGIG MLPLYAAISG LRDGTLVRVL PQYTAQKMNI YALYPSRKFI DAKTRTWVEF LRTHLPKVIA RDEALLAEVD KAQISDGVIP AGASSGADIA TQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatacgtta caaaatatgc gagtgttcgt gcgcgtggtc gaagccggga gtttcaccgg tgccgcgcag tcgctcaatt caacgaccgg cgcaatgtcc cgcgcggttt cggaactcga agctcatctg cggacccggt tgctgaaccg ctccacgcga cggctagcgc tgaccactgc gggcgagcgt tacctgacac gatgtcagca gatacttgca gatgtcagca cagcggaaga agaagccagt tgtgcgcatg agcgcccgac cggcgctttg agaatgcata gctttgccag catcggccag cattacgtgc tgccggctat ctcgcgctat cgcgcgctgt atccggaggt gactgtcgag ctgacgttgt cgcagcgcat gcctgatctc tttgaaggca gcgcggatgt cgcggtgatc ggcgcttcca ctttgcctaa ttcggatctc gtgtcgcttc cgcttggaac gaccttcagc atcttgtgcg cttcgccggc gtatgtgcgc gcccacggcg cgccgcagaa gccggccgat ctggcacacc acgaatgtct gatcctgcat accccggcgt ttccgccgca cgactgggtg ctcgaagggc cgaatggctg cgaaacgatg gaggtgaatg ggcctgtgca cgtgaacatc gccgagtcgc tgatcgtcgc gattcgcgaa ggaatgggca ttggcatgct gccgctctac gcggcaattt ccggattacg tgatggaacg ttagtgcgcg tgttgccgca atataccgcg cagaagatga atatttacgc gctatatccg tcgcgtaagt tcatcgatgc gaagacccgg acttgggtcg aattcttgcg tacgcacctg cccaaagtca ttgcccgcga cgaagcattg ctcgcagaag tcgacaaagc gcagattagc gatggcgtga tacccgctgg cgcaagcagc ggcgcggata tcgcgacgca gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa