SSGCID: Target Page

BuxeA.12800.a

Transcriptional regulator, LysR family

CENTER ID:	BuxeA.12800.a
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.12800.a.A2.GE32016	Mature protein( BuxeA.12800.a )		22	322

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.9140
RefSeq:	YP_556588.1
UniProt:	Q13FA1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRLTSLGVF VAAVEEGSFA AAARRFGLSA AMAGKHVSAL EAELNARLLQ RTTRRLSLTD TGQTYYERCK RILEAFDEAR REASDSQGTA RGVLRVAAPA TFGAMHLGEV VARYLEDHPH VNVEVLLGDR YLDLIEAGVD VAIRIGRLEN PGLVTRRLAL CRMVVCASPA YLKRHGTPRK PDDLMHAQRL VFSEAVSAGD WTLRDGKNRA HVIDGPCRMT ANNTQMLLAS ALAGAGIVYG PTFVFGAHIR RGELVALLPD YRPADLTIQA VYPSARRIPL KVRRFVDYLA EAFGDEPPWD RKEMPWKGAV PGSKVAVERG RI

NT Sequence

ATGGACCGAC TCACAAGCCT CGGCGTTTTC GTCGCCGCTG TCGAGGAAGG CAGCTTTGCC GCGGCGGCAC GCCGCTTCGG GCTATCGGCA GCGATGGCCG GCAAACACGT GAGCGCTCTC GAGGCCGAAC TGAATGCGCG GCTACTCCAG CGCACCACCC GGCGCTTGAG TCTGACCGAT ACTGGCCAGA CTTACTACGA ACGCTGCAAG CGGATTCTCG AAGCCTTCGA CGAAGCGAGG CGGGAAGCGA GCGATTCGCA AGGCACGGCG CGCGGTGTGC TGCGTGTCGC GGCGCCGGCC ACGTTCGGCG CGATGCACCT GGGCGAGGTT GTAGCGCGCT ACCTGGAGGA CCATCCGCAC GTCAACGTGG AAGTGCTGCT CGGAGACCGC TACCTCGATC TCATCGAGGC GGGCGTTGAC GTCGCAATCC GGATAGGACG GCTGGAGAAC CCGGGACTTG TAACGCGCCG GCTTGCGCTG TGCCGAATGG TCGTGTGTGC GTCTCCCGCC TACCTGAAGC GCCATGGAAC GCCGCGCAAG CCGGACGACC TGATGCATGC ACAACGACTT GTGTTCAGCG AGGCCGTGTC GGCCGGAGAC TGGACGCTGC GCGACGGGAA AAACCGCGCG CACGTGATCG ACGGACCTTG CCGGATGACG GCCAACAATA CGCAGATGCT GCTCGCCTCC GCTCTTGCCG GAGCGGGCAT CGTGTACGGT CCGACCTTCG TGTTCGGCGC GCACATCCGG CGCGGCGAAC TCGTGGCGCT GCTGCCGGAC TATCGCCCGG CTGATCTGAC GATTCAAGCA GTTTATCCGA GCGCGCGGCG CATACCGCTC AAAGTGCGAC GGTTCGTCGA TTACCTCGCC GAGGCGTTCG GCGATGAACC GCCTTGGGAT CGCAAGGAGA TGCCTTGGAA GGGGGCTGTT CCAGGCTCGA AAGTGGCCGT TGAGCGGGGG CGAATA

Details for BuxeA.12800.a.A2.GE32016

HARVESTED ON:	4/5/2011
SEQUENCED ON:	4/11/2011
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMAARRFG LSAAMAGKHV SALEAELNAR LLQRTTRRLS LTDTGQTYYE RCKRILEAFD EARREASDSQ GTARGVLRVA APATFGAMHL GEVVARYLED HPHVNVEVLL GDRYLDLIEA GVDVAIRIGR LENPGLVTRR LALCRMVVCA SPAYLKRHGT PRKPDDLMHA QRLVFSEAVS AGDWTLRDGK NRAHVIDGPC RMTANNTQML LASALAGAGI VYGPTFVFGA HIRRGELVAL LPDYRPADLT IQAVYPSARR IPLKVRRFVD YLAEAFGDEP PWDRKEMPWK GAVPGSKVAV ERGRI

Validated NT Sequence

tattatattc gcccccgctc aacggccact ttcgagcctg gaacagcccc cttccaaggc atctccttgc gatcccaagg cggttcatcg ccgaacgcct cggcgaggta atcgacgaac cgtcgcactt tgagcggtat gcgccgcgcg ctcggataaa ctgcttgaat cgtcagatca gccgggcgat agtccggcag cagcgccacg agttcgccgc gccggatgtg cgcgccgaac acgaaggtcg gaccgtacac gatgcccgct ccggcaagag cggaggcgag cagcatctgc gtattgttgg ccgtcatccg gcaaggtccg tcgatcacgt gcgcgcggtt tttcccgtcg cgcagcgtcc agtctccggc cgacacggcc tcgctgaaca caagtcgttg tgcatgcatc aggtcgtccg gcttgcgcgg cgttccatgg cgcttcaggt aggcgggaga cgcacacacg accattcggc acagcgcaag ccggcgcgtt acaagtcccg ggttctccag ccgtcctatc cggattgcga cgtcaacgcc cgcctcgatg agatcgaggt agcggtctcc gagcagcact tccacgttga cgtgcggatg gtcctccagg tagcgcgcta caacctcgcc caggtgcatc gcgccgaacg tggccggcgc cgcgacacgc agcacaccgc gcgccgtgcc ttgcgaatcg ctcgcttccc gcctcgcttc gtcgaaggct tcgagaatcc gcttgcagcg ttcgtagtaa gtctggccag tatcggtcag actcaagcgc cgggtggtgc gctggagtag ccgcgcattc agttcggcct cgagagcgct cacgtgtttg ccggccatcg ctgccgatag cccgaagcgg cgtgccgcca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAARRFGLS AAMAGKHVSA LEAELNARLL QRTTRRLSLT DTGQTYYERC KRILEAFDEA RREASDSQGT ARGVLRVAAP ATFGAMHLGE VVARYLEDHP HVNVEVLLGD RYLDLIEAGV DVAIRIGRLE NPGLVTRRLA LCRMVVCASP AYLKRHGTPR KPDDLMHAQR LVFSEAVSAG DWTLRDGKNR AHVIDGPCRM TANNTQMLLA SALAGAGIVY GPTFVFGAHI RRGELVALLP DYRPADLTIQ AVYPSARRIP LKVRRFVDYL AEAFGDEPPW DRKEMPWKGA VPGSKVAVER GRI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggcacgc cgcttcgggc tatcggcagc gatggccggc aaacacgtga gcgctctcga ggccgaactg aatgcgcggc tactccagcg caccacccgg cgcttgagtc tgaccgatac tggccagact tactacgaac gctgcaagcg gattctcgaa gccttcgacg aagcgaggcg ggaagcgagc gattcgcaag gcacggcgcg cggtgtgctg cgtgtcgcgg cgccggccac gttcggcgcg atgcacctgg gcgaggttgt agcgcgctac ctggaggacc atccgcacgt caacgtggaa gtgctgctcg gagaccgcta cctcgatctc atcgaggcgg gcgttgacgt cgcaatccgg ataggacggc tggagaaccc gggacttgta acgcgccggc ttgcgctgtg ccgaatggtc gtgtgtgcgt ctcccgccta cctgaagcgc catggaacgc cgcgcaagcc ggacgacctg atgcatgcac aacgacttgt gttcagcgag gccgtgtcgg ccggagactg gacgctgcgc gacgggaaaa accgcgcgca cgtgatcgac ggaccttgcc ggatgacggc caacaatacg cagatgctgc tcgcctccgc tcttgccgga gcgggcatcg tgtacggtcc gaccttcgtg ttcggcgcgc acatccggcg cggcgaactc gtggcgctgc tgccggacta tcgcccggct gatctgacga ttcaagcagt ttatccgagc gcgcggcgca taccgctcaa agtgcgacgg ttcgtcgatt acctcgccga ggcgttcggc gatgaaccgc cttgggatcg caaggagatg ccttggaagg gggctgttcc aggctcgaaa gtggccgttg agcgggggcg aatataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa