BuxeA.01673.v

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuxeA.01673.v
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.v.A2.GE34890 Mature protein( BuxeA.01673.v ) 34 509
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.104
RefSeq: YP_556688.1
UniProt: Q146V3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNAPASRRRF VVRPLRRVSL HACALTLASA CALTACTVGP DYVKPTATTA ATYKELDGTG WKPAQPADTF ARGAWWSVYA DPSLDALEQQ VASANQNVQA AEARFRAARA TVAQYRSSFF PLVSATGAYS RARSSENVLH KSTAGLTLND YLVQADASWE PDLWGRVSRS VEGAKAEAQA SAADTQAALL SMQAELATDY FELRGLDQER QLLDDTIEAY RQALELTQHR YTGGIATDAD VAQAQTQLKT TQAQALDLGV QRAQLEHAIA ILIGQPPSTF ALPVAPLAAV PVVAAAGVPS ALLERRPDIA AAERRVAGMN AQIGVATAAF FPNLVLSVTG GLEATNYSQW LLAPSRLWSL GPTLAGTLLD FGGRASVKEQ ARAHYDESVA QYRQTVLTAF GQVEDNLAAL RVLEQEASAQ DDAVTAAQRA LAVVSDRYRN GAITYLDVVV AQTTALTNER DAVSIARRRM AASVALIKAL GGGWDESALP TDEQIVHPSP PASDAAAHG
NT Sequence
ATGAACGCGC CGGCATCCCG TCGCCGTTTC GTCGTGCGGC CGCTGCGGCG CGTCTCGCTG CATGCGTGCG CCTTGACACT CGCAAGCGCG TGCGCACTCA CCGCGTGCAC CGTCGGCCCC GACTACGTGA AGCCGACCGC GACCACCGCC GCCACCTACA AGGAACTCGA CGGCACCGGC TGGAAACCCG CGCAACCCGC CGACACTTTC GCACGCGGCG CTTGGTGGAG CGTCTACGCG GATCCGTCGC TCGACGCGCT CGAACAGCAG GTCGCGAGCG CGAACCAGAA CGTGCAGGCT GCCGAAGCAC GCTTTCGCGC CGCCCGTGCC ACCGTCGCGC AATATCGCTC GAGTTTCTTC CCGCTGGTCA GCGCGACCGG CGCCTATTCG CGCGCGCGTT CATCGGAGAA CGTGCTGCAC AAATCGACCG CGGGTCTCAC GCTGAATGAC TACCTCGTGC AAGCCGACGC GTCGTGGGAA CCCGACCTGT GGGGCCGCGT GTCGCGCAGT GTCGAAGGCG CGAAAGCCGA AGCGCAGGCA AGCGCCGCCG ACACACAAGC CGCGCTGCTG TCGATGCAGG CCGAACTCGC CACCGACTAC TTCGAGCTGC GCGGACTCGA TCAGGAGCGC CAACTGCTCG ACGACACGAT CGAGGCCTAT AGGCAAGCGC TGGAGCTGAC GCAGCACCGC TATACCGGCG GCATCGCCAC GGACGCCGAT GTCGCGCAGG CGCAGACACA ACTGAAAACC ACCCAGGCCC AAGCGCTCGA CCTCGGCGTG CAGCGCGCGC AGCTCGAACA CGCGATCGCG ATCCTGATCG GCCAACCGCC TTCCACGTTT GCATTGCCGG TCGCGCCGCT CGCGGCGGTG CCGGTCGTCG CGGCGGCGGG CGTGCCGTCC GCATTGCTCG AACGGCGCCC CGACATCGCG GCGGCGGAAC GGCGCGTCGC CGGGATGAAC GCGCAGATCG GCGTGGCGAC GGCGGCGTTT TTCCCGAACC TCGTTTTGTC CGTGACGGGC GGCCTCGAAG CGACCAACTA CAGCCAATGG CTGCTGGCGC CGAGCCGGCT GTGGTCGCTC GGGCCGACGT TGGCCGGAAC GCTGCTCGAC TTCGGCGGAC GCGCCTCCGT CAAGGAACAG GCCCGCGCGC ACTACGACGA AAGCGTCGCG CAATACCGGC AGACCGTGCT GACCGCCTTC GGCCAGGTCG AGGACAATCT CGCCGCGCTG CGCGTGCTCG AACAGGAAGC GAGCGCGCAG GACGACGCGG TGACAGCCGC GCAACGTGCG CTGGCGGTCG TATCGGACCG CTACAGGAAC GGCGCGATCA CCTATCTCGA CGTGGTGGTC GCGCAAACCA CCGCGCTCAC CAATGAACGC GATGCCGTAT CGATCGCGCG CCGGCGCATG GCCGCGAGCG TGGCGCTGAT CAAGGCGCTG GGCGGCGGCT GGGACGAGTC CGCGCTGCCG ACGGACGAAC AGATCGTGCA TCCGTCCCCA CCCGCGAGCG ATGCAGCCGC GCACGGG
Details for BuxeA.01673.v.A2.GE34890
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 72
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTACTVGPD YVKPTATTAA TYKELDGTGW KPAQPADTFA RGAWWSVYAD PSLDALEQQV ASANQNVQAA EARFRAARAT VAQYRSSFFP LVSATGAYSR ARSSENVLHK STAGLTLNDY LVQADASWEP DLWGRVSRSV EGAKAEAQAS AADTQAALLS MQAELATDYF ELRGLDQERQ LLDDTIEAYR QALELTQHRY TGGIATDADV AQAQTQLKTT QAQALDLGVQ RAQLEHAIAI LIGQPPSTFA LPVAPLAAVP VVAAAGVPSX LLERRPDIAA AXRRVAGXTX RSAWRXXRFP XPRXXXXRXA XXXPLXPMAA GAXXAXXXXX XXXXXXXXXX XXX
Validated NT Sequence
tttntttaac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaccgc gtgcaccgtc ggccccgact acgtgaagcc gaccgcgacc accgccgcca cctacaagga actcgacggc accggctgga aacccgcgca acccgccgac actttcgcac gcggcgcttg gtggagcgtc tacgcggatc cgtcgctcga cgcgctcgaa cagcaggtcg cgagcgcgaa ccagaacgtg caggctgccg aagcacgctt tcgcgccgcc cgtgccaccg tcgcgcaata tcgctcgagt ttcttcccgc tggtcagcgc gaccggcgcc tattcgcgcg cgcgttcatc ggagaacgtg ctgcacaaat cgaccgcggg tctcacgctg aatgactacc tcgtgcaagc cgacgcgtcg tgggaacccg acctgtgggg ccgcgtgtcg cgcagtgtcg aaggcgcgaa agccgaagcg caggcaagcg ccgccgacac acaagccgcg ctgctgtcga tgcaggccga actcgccacc gactacttcg agctgcgcgg actcgatcag gagcgccaac tgctcgacga cacgatcgag gcctataggc aagcgctgga gctgacgcag caccgctata ccggcggcat cgccacggac gccgatgtcg cgcaggcgca gacacaactg aaaaccaccc aggcccaagc gctcgacctc ggcgtgcagc gcgcgcagct cgaacacgcg atcgcgatcc tgatcggnca accgccttcc acgtttgcat tgccggtcgc gccgctcgcg gcggtgccgg tcgtcgcggc ggcgggcgtg ccgtccncat tgctcgaacg gcgccccgac atcgcggcgg cgnaacggcg cgtcgccggg ntgacncnca gatcggcgtg gcganggngg cgttttccnn aacctcgttt ngnccnnnga cgggnggcnn cgaancnacc actacngccn atggctgcng gcgccnannc ngctgnngnn ncnnnngnnn nttngncgna nnnnnnnnnn nnnnnnnnnn gnnnnnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTACTVGPD YVKPTATTAA TYKELDGTGW KPAQPADTFA RGAWWSVYAD PSLDALEQQV ASANQNVQAA EARFRAARAT VAQYRSSFFP LVSATGAYSR ARSSENVLHK STAGLTLNDY LVQADASWEP DLWGRVSRSV EGAKAEAQAS AADTQAALLS MQAELATDYF ELRGLDQERQ LLDDTIEAYR QALELTQHRY TGGIATDADV AQAQTQLKTT QAQALDLGVQ RAQLEHAIAI LIGQPPSTFA LPVAPLAAVP VVAAAGVPSA LLERRPDIAA AERRVAGMNA QIGVATAAFF PNLVLSVTGG LEATNYSQWL LAPSRLWSLG PTLAGTLLDF GGRASVKEQA RAHYDESVAQ YRQTVLTAFG QVEDNLAALR VLEQEASAQD DAVTAAQRAL AVVSDRYRNG AITYLDVVVA QTTALTNERD AVSIARRRMA ASVALIKALG GGWDESALPT DEQIVHPSPP ASDAAAHG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgcgtgc accgtcggcc ccgactacgt gaagccgacc gcgaccaccg ccgccaccta caaggaactc gacggcaccg gctggaaacc cgcgcaaccc gccgacactt tcgcacgcgg cgcttggtgg agcgtctacg cggatccgtc gctcgacgcg ctcgaacagc aggtcgcgag cgcgaaccag aacgtgcagg ctgccgaagc acgctttcgc gccgcccgtg ccaccgtcgc gcaatatcgc tcgagtttct tcccgctggt cagcgcgacc ggcgcctatt cgcgcgcgcg ttcatcggag aacgtgctgc acaaatcgac cgcgggtctc acgctgaatg actacctcgt gcaagccgac gcgtcgtggg aacccgacct gtggggccgc gtgtcgcgca gtgtcgaagg cgcgaaagcc gaagcgcagg caagcgccgc cgacacacaa gccgcgctgc tgtcgatgca ggccgaactc gccaccgact acttcgagct gcgcggactc gatcaggagc gccaactgct cgacgacacg atcgaggcct ataggcaagc gctggagctg acgcagcacc gctataccgg cggcatcgcc acggacgccg atgtcgcgca ggcgcagaca caactgaaaa ccacccaggc ccaagcgctc gacctcggcg tgcagcgcgc gcagctcgaa cacgcgatcg cgatcctgat cggccaaccg ccttccacgt ttgcattgcc ggtcgcgccg ctcgcggcgg tgccggtcgt cgcggcggcg ggcgtgccgt ccgcattgct cgaacggcgc cccgacatcg cggcggcgga acggcgcgtc gccgggatga acgcgcagat cggcgtggcg acggcggcgt ttttcccgaa cctcgttttg tccgtgacgg gcggcctcga agcgaccaac tacagccaat ggctgctggc gccgagccgg ctgtggtcgc tcgggccgac gttggccgga acgctgctcg acttcggcgg acgcgcctcc gtcaaggaac aggcccgcgc gcactacgac gaaagcgtcg cgcaataccg gcagaccgtg ctgaccgcct tcggccaggt cgaggacaat ctcgccgcgc tgcgcgtgct cgaacaggaa gcgagcgcgc aggacgacgc ggtgacagcc gcgcaacgtg cgctggcggt cgtatcggac cgctacagga acggcgcgat cacctatctc gacgtggtgg tcgcgcaaac caccgcgctc accaatgaac gcgatgccgt atcgatcgcg cgccggcgca tggccgcgag cgtggcgctg atcaaggcgc tgggcggcgg ctgggacgag tccgcgctgc cgacggacga acagatcgtg catccgtccc cacccgcgag cgatgcagcc gcgcacgggt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa