BuxeA.01673.u

MFS efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuxeA.01673.u
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.u.A2.GE34892 Mature protein( BuxeA.01673.u ) 18 502
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.883
RefSeq: YP_557459.1
UniProt: Q143T2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNSQSLSAFA LSSRAAVAAA MTALTLTGCA NYFGMKSDKQ ISSPMQYESS QSLPGEDGQW PSLDWANQFG DQQLPRLIAE ALDGSPSIAQ AQARLAKASS YIETSRAALF PKVNGSYSWT RELYSANAIY PPPFGGTWFS ENNVLASASW DLDLWGKNRQ RLGQAVSQER AAQADMQQAR VTLAASVAST YNQLAQLYAF REIARREIAN RQDIGRITDG RVTAGLDTNV ERQTAAGNIA TSQSNLTDLD GQITVVRYQL GALLGKGPDR GLQIAMPVLT TGDTVALPNN IPADLVARRA DIVAARWQVE AAMHDVKEAK AEFFPDVNLA AGFGFDAFGW GRFLTSASRQ VQLGPAIHLP IFDAGALRSQ LKGRYADFDL DVANYNQTLI NALQDVATQV SSIRSIDQQS GDAQRALDAT SKAYQLAVVR YKAGLSPQLQ VLTADQNLLA AEQTVTSLKM RRRDMQIGLI KALGGGFDAT QTGLVVPADA PASATAATAT AN
NT Sequence
ATGAACTCTC AATCCCTGTC CGCGTTCGCG CTCTCGAGCC GGGCCGCCGT CGCCGCCGCG ATGACGGCGC TCACCCTCAC GGGGTGCGCG AACTACTTCG GCATGAAGAG CGACAAGCAG ATCTCGTCGC CGATGCAGTA TGAGTCCTCG CAGAGTTTGC CCGGCGAAGA CGGCCAATGG CCGTCGCTCG ACTGGGCCAA CCAGTTCGGC GACCAGCAAC TGCCCAGGCT GATCGCCGAA GCGCTTGACG GCAGCCCGTC GATTGCGCAG GCGCAGGCGC GGCTCGCGAA GGCGTCCTCG TATATCGAGA CCTCGCGCGC GGCGCTGTTC CCGAAGGTCA ACGGCAGCTA TTCGTGGACT CGCGAACTGT ACTCCGCCAA TGCGATCTAT CCGCCTCCGT TTGGGGGCAC CTGGTTTAGC GAGAACAACG TGCTCGCAAG CGCGTCGTGG GATCTCGACC TGTGGGGCAA GAACCGCCAG CGCCTCGGCC AGGCCGTATC GCAGGAAAGG GCCGCTCAGG CCGACATGCA GCAGGCGCGC GTGACGCTTG CCGCGTCCGT GGCCAGCACC TACAACCAGC TCGCGCAGTT GTACGCGTTC CGTGAAATCG CCAGGCGCGA AATCGCCAAC CGCCAGGACA TCGGCCGCAT CACCGACGGC CGCGTGACCG CCGGCCTCGA CACCAACGTC GAGCGGCAAA CTGCGGCCGG CAACATCGCG ACCAGCCAGT CGAACCTGAC CGACCTCGAC GGCCAGATCA CCGTCGTGCG CTACCAGTTG GGTGCGCTGC TCGGCAAGGG TCCGGATCGT GGCCTGCAGA TCGCCATGCC GGTGCTGACC ACCGGCGACA CGGTCGCGCT GCCGAACAAT ATTCCGGCCG ATCTGGTAGC GCGCCGCGCG GATATCGTCG CCGCGCGCTG GCAGGTCGAA GCCGCGATGC ACGACGTGAA GGAAGCGAAA GCCGAGTTCT TCCCGGACGT CAATCTCGCG GCCGGCTTCG GTTTCGACGC GTTCGGCTGG GGGCGCTTTC TCACCTCCGC AAGCCGTCAG GTCCAGCTCG GCCCGGCCAT TCACCTGCCG ATCTTCGACG CCGGCGCGCT GCGCTCGCAA TTGAAGGGCC GCTATGCCGA CTTCGACCTG GACGTGGCGA ACTACAACCA GACGCTGATC AACGCGCTGC AGGACGTCGC GACACAAGTG TCGTCGATCC GTTCGATCGA CCAGCAATCA GGCGATGCAC AACGCGCGCT CGACGCAACG AGCAAGGCGT ATCAGCTCGC CGTGGTCCGC TACAAGGCTG GCCTGTCGCC GCAGCTGCAG GTGCTGACCG CCGACCAGAA CCTGCTTGCC GCCGAACAGA CGGTGACGAG CCTGAAGATG CGCCGCCGCG ATATGCAGAT CGGACTCATC AAGGCGCTTG GCGGCGGTTT TGACGCGACG CAGACCGGCC TCGTGGTGCC GGCCGATGCC CCGGCCTCGG CCACCGCCGC GACCGCCACG GCGAAC
Details for BuxeA.01673.u.A2.GE34892
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 57
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAAMTALT LTGCANYFGM KSDKQISSPM QYESSQSLPG EDGQWPSLDW ANQFGDQQLP RLIAEALDGS PSIAQAQARL AKASSYIETS RAALFPKVNG SYSWTRELYS ANAIYPPPFG GTWFSENNVL ASASWDLDLW GKNRQRLGQA VSQERAAQAD MQQARVTLAA SVASTYNQLA QLYAFREIAR REIANRQDIG RITDGRVTAG LDTNVERQTA AGNIATSQSN LTDLDGQITV VRYQLGALLG KGPXRGLXIA MPVLTTGDXV AL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg ccgcgatgac ggcgctcacc ctcacggggt gcgcgaacta cttcggcatg aagagcgaca agcagatctc gtcgccgatg cagtatgagt cctcgcagag tttgcccggc gaagacggcc aatggccgtc gctcgactgg gccaaccagt tcggcgacca gcaactgccc aggctgatcg ccgaagcgct tgacggcagc ccgtcgattg cgcaggcgca ggcgcggctc gcgaaggcgt cctcgtatat cgagacctcg cgcgcggcgc tgttcccgaa ggtcaacggc agctattcgt ggactcgcga actgtactcc gccaatgcga tctatccgcc tccgtttggg ggcacctggt ttagcgagaa caacgtgctc gcaagcgcgt cgtgggatct cgacctgtgg ggcaagaacc gccagcgcct cggccaggcc gtatcgcagg aaagggccgc tcaggccgac atgcagcagg cgcgcgtgac gcttgccgcg tccgtggcca gcacctacaa ccagctcgcg cagttgtacg cgttccgtga aatcgccagg cgcgaaatcg ccaaccgcca ggacatcggc cgcatcaccg acggccgcgt gaccgccggc ctcgacacca acgtcgagcg gcaaactgcg gccggcaaca tcgcgaccag ccagtcgaac ctgaccgacc tcgacggcca gatcaccgtc gtgcgctacc agttgggtgc gctgctcggc aagggtccgg ancgtggcct gcanatcgcc atgccggtgc tgaccaccgg cgacncggtc gcnctgnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAAMTALT LTGCANYFGM KSDKQISSPM QYESSQSLPG EDGQWPSLDW ANQFGDQQLP RLIAEALDGS PSIAQAQARL AKASSYIETS RAALFPKVNG SYSWTRELYS ANAIYPPPFG GTWFSENNVL ASASWDLDLW GKNRQRLGQA VSQERAAQAD MQQARVTLAA SVASTYNQLA QLYAFREIAR REIANRQDIG RITDGRVTAG LDTNVERQTA AGNIATSQSN LTDLDGQITV VRYQLGALLG KGPDRGLQIA MPVLTTGDTV ALPNNIPADL VARRADIVAA RWQVEAAMHD VKEAKAEFFP DVNLAAGFGF DAFGWGRFLT SASRQVQLGP AIHLPIFDAG ALRSQLKGRY ADFDLDVANY NQTLINALQD VATQVSSIRS IDQQSGDAQR ALDATSKAYQ LAVVRYKAGL SPQLQVLTAD QNLLAAEQTV TSLKMRRRDM QIGLIKALGG GFDATQTGLV VPADAPASAT AATATAN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgccgcg atgacggcgc tcaccctcac ggggtgcgcg aactacttcg gcatgaagag cgacaagcag atctcgtcgc cgatgcagta tgagtcctcg cagagtttgc ccggcgaaga cggccaatgg ccgtcgctcg actgggccaa ccagttcggc gaccagcaac tgcccaggct gatcgccgaa gcgcttgacg gcagcccgtc gattgcgcag gcgcaggcgc ggctcgcgaa ggcgtcctcg tatatcgaga cctcgcgcgc ggcgctgttc ccgaaggtca acggcagcta ttcgtggact cgcgaactgt actccgccaa tgcgatctat ccgcctccgt ttgggggcac ctggtttagc gagaacaacg tgctcgcaag cgcgtcgtgg gatctcgacc tgtggggcaa gaaccgccag cgcctcggcc aggccgtatc gcaggaaagg gccgctcagg ccgacatgca gcaggcgcgc gtgacgcttg ccgcgtccgt ggccagcacc tacaaccagc tcgcgcagtt gtacgcgttc cgtgaaatcg ccaggcgcga aatcgccaac cgccaggaca tcggccgcat caccgacggc cgcgtgaccg ccggcctcga caccaacgtc gagcggcaaa ctgcggccgg caacatcgcg accagccagt cgaacctgac cgacctcgac ggccagatca ccgtcgtgcg ctaccagttg ggtgcgctgc tcggcaaggg tccggatcgt ggcctgcaga tcgccatgcc ggtgctgacc accggcgaca cggtcgcgct gccgaacaat attccggccg atctggtagc gcgccgcgcg gatatcgtcg ccgcgcgctg gcaggtcgaa gccgcgatgc acgacgtgaa ggaagcgaaa gccgagttct tcccggacgt caatctcgcg gccggcttcg gtttcgacgc gttcggctgg gggcgctttc tcacctccgc aagccgtcag gtccagctcg gcccggccat tcacctgccg atcttcgacg ccggcgcgct gcgctcgcaa ttgaagggcc gctatgccga cttcgacctg gacgtggcga actacaacca gacgctgatc aacgcgctgc aggacgtcgc gacacaagtg tcgtcgatcc gttcgatcga ccagcaatca ggcgatgcac aacgcgcgct cgacgcaacg agcaaggcgt atcagctcgc cgtggtccgc tacaaggctg gcctgtcgcc gcagctgcag gtgctgaccg ccgaccagaa cctgcttgcc gccgaacaga cggtgacgag cctgaagatg cgccgccgcg atatgcagat cggactcatc aaggcgcttg gcggcggttt tgacgcgacg cagaccggcc tcgtggtgcc ggccgatgcc ccggcctcgg ccaccgccgc gaccgccacg gcgaactaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa