BuxeA.01673.t

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuxeA.01673.t
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.t.A2.GE32010 Mature protein( BuxeA.01673.t ) 21 489
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.1114
RefSeq: YP_557675.1
UniProt: Q142W6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKVRLPALL ASVMALSACS LAPQYQVPPT PVAAQYKTVG PWTAAQPADR IDRNGWWKMY GDAQLDTLET SLLANNTDLR AAYAHYAQAQ AFVAQVESGL YPSISASAVP QRDRQSDNRP LRAGGPNDYN SVTLGGEVNY DLDLWGRVRD SVVAGKDEAQ ATKADLASVQ LSLQVTLADS YIRLRGLDQQ TDLLNETVAA YAKALQLTQT LHNGGIVSGL DVSRAQTQLS SAKSQLSQNL AQRALIEHAI AVLVGASASE FSLPQQTTAI ALLAIPTGLP STLLQRRPDV AAAERRVAEA NAKIGVARAA YFPDITLSLQ GGVQSAAYAG LVSAPNLFWA IGPQLVQYVF DGGYRRAQLD AAKAATEEAG ERYRGVVLSA FQQVEDNLAL LSYLGTALGE QRDAASAAQY SVDLALRQYR QGTVGYLDVV QAQTVQLDAQ RSLLDIQTRQ LSANVQLLHA LGGGWSSDEL ARVAATPALV ASSTQHAAN
NT Sequence
ATGGCTAAGG TTCGACTTCC AGCGCTGCTC GCGAGCGTGA TGGCATTGTC AGCGTGCTCG CTCGCCCCGC AATACCAGGT TCCGCCCACA CCCGTCGCCG CGCAATACAA GACCGTTGGA CCGTGGACCG CCGCGCAGCC CGCGGACCGG ATAGACCGCA ACGGCTGGTG GAAAATGTAT GGCGACGCCC AGCTCGACAC ACTCGAAACC AGCCTGCTGG CAAACAACAC CGATCTGCGC GCCGCTTACG CGCACTATGC GCAAGCGCAG GCGTTCGTGG CGCAGGTCGA GTCGGGTCTG TACCCGTCCA TCAGCGCCAG CGCCGTGCCG CAGCGCGACC GCCAGTCCGA CAATCGCCCG CTGCGCGCGG GAGGCCCCAA CGACTACAAC TCCGTCACGC TCGGCGGCGA GGTCAATTAC GACCTCGATC TGTGGGGCCG CGTGCGCGAC TCCGTTGTTG CGGGCAAGGA TGAAGCGCAG GCGACCAAGG CCGATCTGGC TTCCGTGCAA TTGAGCCTGC AAGTCACCCT GGCGGACAGT TATATCCGCT TGCGAGGCCT CGATCAGCAA ACCGATCTGT TGAACGAGAC AGTGGCGGCC TACGCGAAGG CGCTGCAACT CACGCAGACC CTGCACAACG GCGGGATCGT GTCGGGCCTC GACGTCTCGC GCGCGCAGAC CCAGTTGTCG TCGGCGAAGT CGCAGTTGTC GCAGAACCTG GCGCAACGCG CGCTCATTGA ACATGCGATC GCCGTACTGG TCGGCGCCTC CGCGTCCGAA TTCAGCCTGC CGCAGCAGAC CACCGCGATT GCGTTGCTGG CCATTCCGAC CGGCCTGCCT TCCACACTGC TGCAGCGCCG GCCCGACGTC GCCGCAGCGG AGCGGCGCGT CGCGGAGGCC AACGCGAAGA TCGGCGTGGC GCGGGCGGCC TACTTCCCGG ACATCACGTT GAGCCTGCAA GGCGGCGTGC AAAGCGCGGC GTATGCCGGG CTCGTGAGCG CACCCAACCT GTTCTGGGCC ATCGGTCCGC AACTCGTGCA ATACGTGTTC GACGGAGGCT ACCGCCGCGC TCAACTCGAC GCCGCCAAAG CGGCCACCGA GGAAGCCGGA GAACGTTACC GTGGCGTGGT GCTGTCCGCT TTCCAGCAGG TCGAAGACAA TCTGGCCTTG CTGTCCTACC TCGGCACTGC ACTCGGAGAA CAACGCGACG CCGCGAGCGC CGCGCAGTAC TCGGTGGATC TGGCACTCCG TCAATACCGC CAGGGCACCG TGGGTTACCT CGATGTCGTG CAGGCGCAAA CCGTTCAGCT CGACGCGCAG CGCAGCCTGC TCGACATCCA GACCCGCCAG CTCAGCGCGA ACGTGCAGTT GCTGCATGCG CTCGGCGGCG GATGGTCGAG CGATGAACTG GCGAGGGTGG CTGCCACGCC GGCACTCGTC GCGAGTTCGA CGCAGCATGC CGCCAAC
Details for BuxeA.01673.t.A2.GE32010
HARVESTED ON: 4/5/2011
SEQUENCED ON: 4/11/2011
EXPECTED MW: 52kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 71
PERCENT COVERAGE: 81
Validated AA Sequence
MFXXLXXXXX XXXXXGXXXS STXGPGSMLA PQYQVPPTPV AAQYKTVGPW TAAQPADRID RNGWWKMYGD AQLDTLETSL LANNTDLRAA YAHYAQAQAF VAQVESGLYP SISASAVPQR DRQSDNRPLR AGGPNDYNSV TLGGEVNYDL DLWGRVRDSV VAGKDEAQAT KADLASVQLS LQVTLADSYI RLRGLDQQTD LLNETVAAYA KALQLTQTLH NGGIVSGLDV SRAQTQLSSA KSQLSXNLAQ RALIEHAIAV LVGASASEFS LPXXTTAIAL LAIPXGXXST LLQRXPTSPX RXGXSRXPTR XXRXXGGXXX GXXVXPARXX XSAXXXXGXX XXXXXXGXXX XXXXXXXTXX XXXXXRXXXX XXRXXXXXXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncagccatgt ttnnnnnnct ccnntnnnnn annnnnannn nannnnnann nggntnccnn gnnagctcaa cccngggtcc tggttcgatg ctcgccccgc aataccaggt tccgcccaca cccgtcgccg cgcaatacaa gaccgttgga ccgtggaccg ccgcgcagcc cgcggaccgg atagaccgca acggctggtg gaaaatgtat ggcgacgccc agctcgacac actcgaaacc agcctgctgg caaacaacac cgatctgcgc gccgcttacg cgcactatgc gcaagcgcag gcgttcgtgg cgcaggtcga gtcgggtctg tacccgtcca tcagcgccag cgccgtgccg cagcgcgacc gccagtccga caatcgcccg ctgcgcgcgg gaggccccaa cgactacaac tccgtcacgc tcggcggcga ggtcaattac gacctcgatc tgtggggccg cgtgcgcgac tccgttgttg cgggcaagga tgaagcgcag gcgaccaagg ccgatctggc ttccgtgcaa ttgagcctgc aagtcaccct ggcggacagt tatatccgct tgcgaggcct cgatcagcaa accgatctgt tgaacgagac agtggcggcc tacgcgaagg cgctgcaact cacgcagacc ctgcacaacg gcgggatcgt gtcgggcctc gacgtctcgc gcgcgcagac ccagttgtcg tcggcgaagt cgcagttgtc gcanaacctg gcgcaacgcg cgctcattga acatgcgatc gccgtactgg tcggcgcctc cgcgtccgaa ttcagcctgc cgcancnnac caccgcgatt gcgttgctgg ccattccnan cgggcntgnt tccacactgc tgcagcgnng cccgacgtcg ccgcancgna ncggcncgtc gcgnangcca acgcganatn ggcgnngngn gggcggncnn nttcncggnn ntcangtnna ncctgcaagg cngnntgnaa gcgcggnntt nngncngggn ntcntnancn nnncnnnntn nncnggnncn nnnnnnnnna nnncaanncn nnnnncnacg gnnnnnncnn ccnnnnnnan ncgannncnn nnnaanngnc nccgnngnna ncnnnnnnnn nnnnccnnnn nnngnnnnnn nnntnnnnnn nnnntnnana nnnatnnggn nnnnnnnnnn c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLAPQYQVP PTPVAAQYKT VGPWTAAQPA DRIDRNGWWK MYGDAQLDTL ETSLLANNTD LRAAYAHYAQ AQAFVAQVES GLYPSISASA VPQRDRQSDN RPLRAGGPND YNSVTLGGEV NYDLDLWGRV RDSVVAGKDE AQATKADLAS VQLSLQVTLA DSYIRLRGLD QQTDLLNETV AAYAKALQLT QTLHNGGIVS GLDVSRAQTQ LSSAKSQLSQ NLAQRALIEH AIAVLVGASA SEFSLPQQTT AIALLAIPTG LPSTLLQRRP DVAAAERRVA EANAKIGVAR AAYFPDITLS LQGGVQSAAY AGLVSAPNLF WAIGPQLVQY VFDGGYRRAQ LDAAKAATEE AGERYRGVVL SAFQQVEDNL ALLSYLGTAL GEQRDAASAA QYSVDLALRQ YRQGTVGYLD VVQAQTVQLD AQRSLLDIQT RQLSANVQLL HALGGGWSSD ELARVAATPA LVASSTQHAA N
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctcgccccg caataccagg ttccgcccac acccgtcgcc gcgcaataca agaccgttgg accgtggacc gccgcgcagc ccgcggaccg gatagaccgc aacggctggt ggaaaatgta tggcgacgcc cagctcgaca cactcgaaac cagcctgctg gcaaacaaca ccgatctgcg cgccgcttac gcgcactatg cgcaagcgca ggcgttcgtg gcgcaggtcg agtcgggtct gtacccgtcc atcagcgcca gcgccgtgcc gcagcgcgac cgccagtccg acaatcgccc gctgcgcgcg ggaggcccca acgactacaa ctccgtcacg ctcggcggcg aggtcaatta cgacctcgat ctgtggggcc gcgtgcgcga ctccgttgtt gcgggcaagg atgaagcgca ggcgaccaag gccgatctgg cttccgtgca attgagcctg caagtcaccc tggcggacag ttatatccgc ttgcgaggcc tcgatcagca aaccgatctg ttgaacgaga cagtggcggc ctacgcgaag gcgctgcaac tcacgcagac cctgcacaac ggcgggatcg tgtcgggcct cgacgtctcg cgcgcgcaga cccagttgtc gtcggcgaag tcgcagttgt cgcagaacct ggcgcaacgc gcgctcattg aacatgcgat cgccgtactg gtcggcgcct ccgcgtccga attcagcctg ccgcagcaga ccaccgcgat tgcgttgctg gccattccga ccggcctgcc ttccacactg ctgcagcgcc ggcccgacgt cgccgcagcg gagcggcgcg tcgcggaggc caacgcgaag atcggcgtgg cgcgggcggc ctacttcccg gacatcacgt tgagcctgca aggcggcgtg caaagcgcgg cgtatgccgg gctcgtgagc gcacccaacc tgttctgggc catcggtccg caactcgtgc aatacgtgtt cgacggaggc taccgccgcg ctcaactcga cgccgccaaa gcggccaccg aggaagccgg agaacgttac cgtggcgtgg tgctgtccgc tttccagcag gtcgaagaca atctggcctt gctgtcctac ctcggcactg cactcggaga acaacgcgac gccgcgagcg ccgcgcagta ctcggtggat ctggcactcc gtcaataccg ccagggcacc gtgggttacc tcgatgtcgt gcaggcgcaa accgttcagc tcgacgcgca gcgcagcctg ctcgacatcc agacccgcca gctcagcgcg aacgtgcagt tgctgcatgc gctcggcggc ggatggtcga gcgatgaact ggcgagggtg gctgccacgc cggcactcgt cgcgagttcg acgcagcatg ccgccaacta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a