BuxeA.01673.s

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuxeA.01673.s
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.s.A2.GE32009 Mature protein( BuxeA.01673.s ) 28 516
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.1600
RefSeq: YP_558133.1
UniProt: Q141A8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTFFSTKKG FTGSVLLGAL MVLSGCSLAP TYEVPSTPTT ATFKEAPQQP LSAQEAGTWK TAQPSEDVAR GEWWKVFDDA KLNDLEQQAL DANQNLKAAA ARVNEARAMN QAARAGLFPS LDAGFGPTRQ RVSAASLFEP DGTNVPQRTF WRAQASASYE VDLFGRVAST VDAARADTQR SEALFRSVML ALQADVAQNY FALRELDAES EVFRNAVELR QHALKLVQRR YTEGEITELD LSRAKAELAS AQSDAMTVQR LRAASEHSLA VLLGKAPAEF SMAADPIRPV NLRVPPGLPS SLLERRPDIA AAERAMAAAN SRIGIAKAAF FPSLTITGTG GFESATIGDL FKWSSRAFLL GPLAGTALNI PIFDGGRRKG NLANARAVYE EDVANYRQQV LVAFREVEDN LSDMRILEDQ TRTQNEAVKA SQRAADLSRS QYTEGAVNYL DVIDAERTVL QARRAAVQLQ GVQAASTVNL IRALGGGWGD VVPQPAQPAV GQVAPPASVQ TAIAQK
NT Sequence
ATGAAAACAT TTTTTTCGAC GAAGAAAGGC TTTACTGGCT CCGTCCTGCT GGGCGCCCTG ATGGTTCTGT CCGGGTGTTC ACTCGCACCG ACGTACGAGG TGCCGTCGAC ACCGACGACG GCGACCTTCA AGGAAGCACC GCAGCAGCCG CTGTCGGCGC AAGAAGCGGG CACATGGAAG ACGGCGCAAC CATCGGAAGA CGTCGCCCGC GGCGAATGGT GGAAGGTGTT CGATGACGCG AAGCTGAACG ACCTCGAACA GCAGGCGCTG GACGCGAACC AGAACCTGAA AGCGGCGGCG GCCCGTGTGA ACGAAGCACG GGCAATGAAT CAGGCTGCGC GCGCGGGTCT GTTCCCCTCG CTCGATGCCG GTTTCGGGCC GACGAGGCAG CGGGTCTCGG CCGCTTCCCT CTTTGAGCCG GACGGCACGA ATGTGCCACA GCGGACGTTC TGGCGCGCTC AGGCGAGCGC TTCGTATGAA GTCGACCTGT TTGGTCGTGT CGCGTCGACG GTCGATGCGG CGCGCGCCGA TACACAGCGC AGCGAGGCGC TCTTTCGCTC GGTAATGCTT GCCCTCCAGG CGGATGTCGC GCAGAACTAT TTTGCGCTTC GCGAGCTCGA CGCCGAATCC GAGGTCTTCA GGAACGCTGT CGAGCTCCGA CAGCACGCCT TGAAGCTGGT TCAGCGCCGC TACACGGAAG GCGAAATCAC CGAGCTCGAC CTCTCGCGTG CGAAAGCTGA ACTGGCCTCT GCCCAATCCG ACGCGATGAC CGTACAACGT CTTCGCGCCG CGTCCGAGCA TAGCCTGGCA GTGCTGCTTG GCAAGGCGCC GGCCGAGTTC TCTATGGCGG CTGATCCCAT CAGGCCTGTC AACCTGCGTG TGCCGCCAGG ACTGCCCTCG TCGCTGCTCG AACGGCGGCC GGACATCGCC GCCGCCGAGC GAGCGATGGC CGCCGCGAAC TCGCGCATTG GTATCGCAAA GGCCGCGTTC TTTCCGTCAC TCACTATCAC GGGTACGGGT GGCTTCGAAT CCGCAACGAT CGGCGACCTC TTCAAATGGA GCAGCCGTGC GTTCCTGCTC GGTCCTCTTG CCGGTACGGC GCTGAATATT CCGATCTTTG ACGGCGGCCG CCGCAAAGGC AACCTCGCGA ACGCTCGGGC TGTCTACGAA GAGGACGTCG CGAATTACCG GCAGCAGGTG CTCGTAGCTT TCCGCGAAGT CGAAGACAAC CTGTCCGACA TGCGCATTCT GGAAGACCAG ACCAGGACGC AGAACGAAGC AGTCAAGGCA TCGCAACGTG CAGCGGACCT CTCGCGCTCG CAGTACACCG AGGGCGCGGT GAACTATCTG GACGTCATCG ATGCGGAGCG CACCGTCCTG CAGGCACGCC GCGCGGCGGT GCAACTGCAG GGTGTGCAGG CGGCTTCCAC GGTCAATCTG ATTCGTGCGC TTGGCGGCGG CTGGGGTGAC GTGGTTCCCC AGCCTGCCCA ACCGGCCGTG GGACAGGTCG CGCCCCCCGC TTCCGTGCAA ACAGCAATCG CACAGAAA
Details for BuxeA.01673.s.A2.GE32009
HARVESTED ON: 4/5/2011
SEQUENCED ON: 4/11/2011
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 65
Validated AA Sequence
MLAPTYEVPS TPTTATFKEA PQQPLSAQEA GTWKTAQPSE DVARGEWWKV FDDAKLNDLE QQALDANXNL KAAAARVNEA RAMNQAARAG LFPSLDAGFG PTRQRVSAAS LFEPDGTNVP QRTFWRAQAS ASYEVDLFGR VASTVDAARA DTQRSEALFR SVMLALQADV AQNYFALREL DAESEVFRNA VELRQHALKL VQRRYTEGEI TELDLSRAKA ELASAQSDAM TVQRLRXASX HSLAVLLXKA PXEFSMAADP SXQPACXART ALVAARRPDX AXXXXXXAAA XSXXXXKXXF FPSLXXXGXX FXSAXXXTXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
gnnnnnnngc catgtttnnn nnnctcnngn nnancnnnan nnnnnnnnaa nnngggtncn nngnnagctc anacccnggg tcctggttcg atgctcgcac cgacgtacga ggtgccgtcg acaccgacga cggcgacctt caaggaagca ccgcagcagc cgctgtcggc gcaagaagcg ggcacatgga agacggcgca accatcggaa gacgtcgccc gcggcgaatg gtggaaggtg ttcgatgacg cgaagctgaa cgacctcgaa cagcaggcgc tggacgcgaa ccanaacctg aaagcggcgg cggcccgtgt gaacgaagca cgggcaatga atcaggctgc gcgcgcgggt ctgttcccct cgctcgatgc cggtttcggg ccgacgaggc agcgggtctc ggccgcttcc ctctttgagc cggacggcac gaatgtgcca cagcggacgt tctggcgcgc tcaggcgagc gcttcgtatg aagtcgacct gtttggtcgt gtcgcgtcga cggtcgatgc ggcgcgcgcc gatacacagc gcagcgaggc gctctttcgc tcggtaatgc ttgccctcca ggcggatgtc gcgcagaact attttgcgct tcgcgagctc gacgccgaat ccgaggtctt caggaacgct gtcgagctcc gacagcacgc cttgaagctg gttcagcgcc gctacacgga aggcgaaatc accgagctcg acctctcgcg tgcgaaagct gaactggcct ctgcccaatc cgacgcgatg accgtacaac gtcttcgcnn cgcgtccnag catagcctgg cagtgctgct nngcaaggcg ccnnncgagt tctctatggc ggctgatccn tcnngtcaac ctgcgtgtnn cgccaggact gccctcgtcg ctgctcgaag gccggacntc gccgncgncn anngancnan ggccgccgcg aantcgcnnn ggtntngnaa agncncgttc tttccgtcac tcannannnn ngggngnngn ttcnaatccg cannannngn nacntnntnn nanngnnnca nccnnnnnnt nnnngntnnn nncnncntgn nggnnnggnn ctgannnncn anttnnnngn nnnnccnnca annnnnnnnc nnnannnnnn nnngnnnnnn aannnnannn nnnnnnnttt nnnnnnnnnn nnncnnnngn cntnnnnnnn nnnnnnngnn nncnnnnnna nnnnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLAPTYEVP STPTTATFKE APQQPLSAQE AGTWKTAQPS EDVARGEWWK VFDDAKLNDL EQQALDANQN LKAAAARVNE ARAMNQAARA GLFPSLDAGF GPTRQRVSAA SLFEPDGTNV PQRTFWRAQA SASYEVDLFG RVASTVDAAR ADTQRSEALF RSVMLALQAD VAQNYFALRE LDAESEVFRN AVELRQHALK LVQRRYTEGE ITELDLSRAK AELASAQSDA MTVQRLRAAS EHSLAVLLGK APAEFSMAAD PIRPVNLRVP PGLPSSLLER RPDIAAAERA MAAANSRIGI AKAAFFPSLT ITGTGGFESA TIGDLFKWSS RAFLLGPLAG TALNIPIFDG GRRKGNLANA RAVYEEDVAN YRQQVLVAFR EVEDNLSDMR ILEDQTRTQN EAVKASQRAA DLSRSQYTEG AVNYLDVIDA ERTVLQARRA AVQLQGVQAA STVNLIRALG GGWGDVVPQP AQPAVGQVAP PASVQTAIAQ K
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctcgcaccg acgtacgagg tgccgtcgac accgacgacg gcgaccttca aggaagcacc gcagcagccg ctgtcggcgc aagaagcggg cacatggaag acggcgcaac catcggaaga cgtcgcccgc ggcgaatggt ggaaggtgtt cgatgacgcg aagctgaacg acctcgaaca gcaggcgctg gacgcgaacc agaacctgaa agcggcggcg gcccgtgtga acgaagcacg ggcaatgaat caggctgcgc gcgcgggtct gttcccctcg ctcgatgccg gtttcgggcc gacgaggcag cgggtctcgg ccgcttccct ctttgagccg gacggcacga atgtgccaca gcggacgttc tggcgcgctc aggcgagcgc ttcgtatgaa gtcgacctgt ttggtcgtgt cgcgtcgacg gtcgatgcgg cgcgcgccga tacacagcgc agcgaggcgc tctttcgctc ggtaatgctt gccctccagg cggatgtcgc gcagaactat tttgcgcttc gcgagctcga cgccgaatcc gaggtcttca ggaacgctgt cgagctccga cagcacgcct tgaagctggt tcagcgccgc tacacggaag gcgaaatcac cgagctcgac ctctcgcgtg cgaaagctga actggcctct gcccaatccg acgcgatgac cgtacaacgt cttcgcgccg cgtccgagca tagcctggca gtgctgcttg gcaaggcgcc ggccgagttc tctatggcgg ctgatcccat caggcctgtc aacctgcgtg tgccgccagg actgccctcg tcgctgctcg aacggcggcc ggacatcgcc gccgccgagc gagcgatggc cgccgcgaac tcgcgcattg gtatcgcaaa ggccgcgttc tttccgtcac tcactatcac gggtacgggt ggcttcgaat ccgcaacgat cggcgacctc ttcaaatgga gcagccgtgc gttcctgctc ggtcctcttg ccggtacggc gctgaatatt ccgatctttg acggcggccg ccgcaaaggc aacctcgcga acgctcgggc tgtctacgaa gaggacgtcg cgaattaccg gcagcaggtg ctcgtagctt tccgcgaagt cgaagacaac ctgtccgaca tgcgcattct ggaagaccag accaggacgc agaacgaagc agtcaaggca tcgcaacgtg cagcggacct ctcgcgctcg cagtacaccg agggcgcggt gaactatctg gacgtcatcg atgcggagcg caccgtcctg caggcacgcc gcgcggcggt gcaactgcag ggtgtgcagg cggcttccac ggtcaatctg attcgtgcgc ttggcggcgg ctggggtgac gtggttcccc agcctgccca accggccgtg ggacaggtcg cgccccccgc ttccgtgcaa acagcaatcg cacagaaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a