SSGCID: Target Page

BuxeA.01673.o

Aromatic acid efflux system, outer membrane lipoprotein

CENTER ID:	BuxeA.01673.o
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.01673.o.A2.GE32005	Mature protein( BuxeA.01673.o )		24	514

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.01673.o.A2.PS01372	Mature protein( BuxeA.01673.o )		24	514

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.3226
RefSeq:	YP_559659.1
UniProt:	Q13WD2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQSPVPKGIA AAAVLTILLT IAGCASTGGV APQASGVQPA SLDAGNAIRA ANADAQWPSV DWWRAYNDPQ LNEWIDAAQA GNPSLAAAQA RVREAMSMAG VARSALSPQV NGNLSVQRQK WSDNVYYGPG PLAGEQSWNN TGTLGLSYHL DIWGKDRNAA ERALDAAHAS AADARAAQLE LEGNVVRTYI EMSMNYALLD IAKSTLQQQQ QIVDLASRRL KGGIGTQLEV SQAETPLPEY ERQIDEIEEK IALGRNQLAA LAGKGPGAGD SIQRPTLSLN TPAGLPSALP ADLIGHRPDV VAARWSVAAQ ARGIDVAKAD FYPDINLLAS IGGYAAMGPL FQFLKNPSHS WSGGPALSLP ILDGGRLRSQ LGAASAGYDE AVDRYNQSIV GALKDISDQV IRIRSLATQA EDADRSVATA RKNYDLSREG YRRGLTDYLN VLIAQNQLLH AQEGVAKIQA ERLGAHASLV TALGGGLDDP ANGPLANETL PAHGKGKTVA PGSTASAEPA RSGS

NT Sequence

GTGCAGTCTC CGGTACCTAA AGGGATCGCC GCAGCCGCGG TTCTTACGAT CCTATTAACA ATCGCCGGCT GTGCCAGTAC CGGAGGCGTC GCGCCGCAAG CGAGCGGAGT CCAGCCCGCT TCGCTCGACG CCGGCAACGC GATCCGCGCC GCCAATGCGG ACGCCCAATG GCCGTCCGTC GACTGGTGGC GCGCCTACAA CGATCCGCAA TTGAACGAGT GGATCGACGC GGCACAGGCC GGCAATCCGA GCCTCGCGGC CGCCCAGGCA CGCGTGCGCG AAGCCATGTC GATGGCGGGC GTCGCGCGTT CGGCGTTGTC GCCGCAAGTC AACGGCAACC TGTCGGTCCA GCGCCAGAAG TGGTCCGACA ATGTCTACTA CGGCCCCGGC CCGCTCGCGG GCGAGCAATC GTGGAACAAC ACCGGCACGC TCGGCCTGTC GTATCACCTC GATATCTGGG GCAAGGACAG GAACGCCGCC GAGCGCGCGC TCGATGCCGC CCACGCGAGC GCGGCGGACG CCCGCGCCGC CCAGCTCGAA CTGGAAGGCA ACGTGGTGCG CACATACATC GAGATGTCGA TGAACTACGC GCTGCTCGAC ATCGCCAAGT CCACCTTGCA GCAGCAGCAA CAGATCGTGG ATCTGGCCAG CCGACGCCTG AAAGGCGGCA TCGGCACGCA ACTGGAAGTG AGCCAGGCCG AAACGCCGCT GCCGGAATAC GAACGCCAGA TCGACGAGAT CGAAGAAAAG ATCGCGCTTG GCCGCAATCA GCTGGCCGCG CTGGCCGGCA AAGGTCCGGG CGCGGGCGAT TCGATCCAGC GCCCGACGCT TTCGCTGAAC ACGCCGGCCG GGCTGCCGAG CGCATTGCCC GCGGACCTGA TCGGCCATCG GCCGGACGTG GTGGCGGCGC GCTGGTCGGT CGCGGCGCAG GCGCGCGGTA TCGACGTCGC CAAGGCGGAC TTCTATCCGG ACATCAACCT GCTGGCGTCG ATCGGCGGAT ATGCGGCAAT GGGGCCGCTG TTCCAGTTCC TGAAGAATCC GTCGCACAGC TGGAGCGGCG GGCCGGCGCT GTCGCTGCCG ATCCTCGACG GCGGCCGCCT GCGTTCGCAG CTCGGCGCGG CTTCGGCGGG CTATGACGAA GCCGTCGACC GTTACAACCA GTCGATCGTC GGCGCGCTCA AGGACATTTC CGATCAGGTG ATCCGGATCC GCTCGCTTGC GACCCAGGCC GAGGACGCCG ACCGTTCGGT CGCGACGGCC CGCAAGAACT ACGATCTGTC GCGCGAAGGT TACCGGCGCG GGCTGACCGA CTATCTGAAC GTGCTGATCG CGCAAAACCA GTTGCTGCAC GCGCAGGAAG GCGTGGCGAA GATTCAGGCC GAACGGCTCG GCGCGCATGC GTCGCTGGTC ACGGCACTGG GCGGCGGACT CGACGACCCG GCCAACGGTC CGCTGGCGAA CGAGACGCTG CCGGCGCACG GCAAGGGTAA GACGGTGGCG CCCGGTTCGA CCGCCAGCGC CGAGCCGGCC AGGTCAGGAA GC

Details for BuxeA.01673.o.A2.GE32005

HARVESTED ON:	4/5/2011
SEQUENCED ON:	4/11/2011
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	81

Validated AA Sequence

MSMAGVARSA LSPQVNGNLS VQRQKWSDNV YYGPGPLAGE QSWNNTGTLG LSYHLXIWGK DRNAAERALD AAHASAADAR AAQLELEGNV VRTYIEMSMN YALLDIAKST LQQQQQIVDL XXRRLKGGIG TQLEVSQAET PLPEYERQID EIEEKIALGR NQLAALAGKG PGAGDSIQRP TLSLNTPAGL PSALPADLIG HRPDVVAARW SVAAQARGID VAKADFYPDI NLLASIGGYA AMGPLFQFLK NPSHSWSGGP ALSLPILDGG RLRSQLGAAS AGYDEAVDRY NQSIVGALKD ISDQVIRIRS LATQAEDADR SVATARKNYD LSREGYRRGL TDYLNVLIAQ NQLLHAQEGV AKIQAERLGA HASLVTALGG GLDDPANGPL ANETLPAHGK GKTVAPGSTA SAEPARSG

Validated NT Sequence

gcttcctgac ctggccggct cggcgctggc ggtcgaaccg ggcgccaccg tcttaccctt gccgtgcgcc ggcagcgtct cgttcgccag cggaccgttg gccgggtcgt cgagtccgcc gcccagtgcc gtgaccagcg acgcatgcgc gccgagccgt tcggcctgaa tcttcgccac gccttcctgc gcgtgcagca actggttttg cgcgatcagc acgttcagat agtcggtcag cccgcgccgg taaccttcgc gcgacagatc gtagttcttg cgggccgtcg cgaccgaacg gtcggcgtcc tcggcctggg tcgcaagcga gcggatccgg atcacctgat cggaaatgtc cttgagcgcg ccgacgatcg actggttgta acggtcgacg gcttcgtcat agcccgccga agccgcgccg agctgcgaac gcaggcggcc gccgtcgagg atcggcagcg acagcgccgg cccgccgctc cagctgtgcg acggattctt caggaactgg aacagcggcc ccattgccgc atatccgccg atcgacgcca gcaggttgat gtccggatag aagtccgcct tggcgacgtc gataccgcgc gcctgcgccg cgaccgacca gcgcgccgcc accacgtccg gccgatggcc gatcaggtcc gcgggcaatg cgctcggcag cccggccggc gtgttcagcg aaagcgtcgg gcgctggatc gaatcgcccg cgcccggacc tttgccggcc agcgcggcca gctgattgcg gccaagcgcg atcttttctt cgatctcgtc gatctggcgt tcgtattccg gcagcggcgt ttcggcctgg ctcacttcca gttgcgtgcc gatgccgcct ttcaggcgtc ggcngnccag atccacgatc tgttgctgct gctgcaaggt ggacttggcg atgtcgagca gcgcgtagtt catcgacatc tcgatgtatg tgcgcaccac gttgccttcc agttcgagct gggcggcgcg ggcgtccgcc gcgctcgcgt gggcggcatc gagcgcgcgc tcggcggcgt tcctgtcctt gccccagata tngaggtgat acgacaggcc gagcgtgccg gtgttgttcc acgattgctc gcccgcgagc gggccggggc cgtagtagac attgtcggac cacttctggc gctggaccga caggttgccg ttgacttgcg gcgacaacgc cgaacgcgcg acgcccgcca tcgacatggc ttcgcgcacg cgtgcctggg cggccgcgag gctcggattg ccggcctgtg ccgcgtcgat ccactcgttc aattgcgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMCASTGGVA PQASGVQPAS LDAGNAIRAA NADAQWPSVD WWRAYNDPQL NEWIDAAQAG NPSLAAAQAR VREAMSMAGV ARSALSPQVN GNLSVQRQKW SDNVYYGPGP LAGEQSWNNT GTLGLSYHLD IWGKDRNAAE RALDAAHASA ADARAAQLEL EGNVVRTYIE MSMNYALLDI AKSTLQQQQQ IVDLASRRLK GGIGTQLEVS QAETPLPEYE RQIDEIEEKI ALGRNQLAAL AGKGPGAGDS IQRPTLSLNT PAGLPSALPA DLIGHRPDVV AARWSVAAQA RGIDVAKADF YPDINLLASI GGYAAMGPLF QFLKNPSHSW SGGPALSLPI LDGGRLRSQL GAASAGYDEA VDRYNQSIVG ALKDISDQVI RIRSLATQAE DADRSVATAR KNYDLSREGY RRGLTDYLNV LIAQNQLLHA QEGVAKIQAE RLGAHASLVT ALGGGLDDPA NGPLANETLP AHGKGKTVAP GSTASAEPAR SGS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgtgccagt accggaggcg tcgcgccgca agcgagcgga gtccagcccg cttcgctcga cgccggcaac gcgatccgcg ccgccaatgc ggacgcccaa tggccgtccg tcgactggtg gcgcgcctac aacgatccgc aattgaacga gtggatcgac gcggcacagg ccggcaatcc gagcctcgcg gccgcccagg cacgcgtgcg cgaagccatg tcgatggcgg gcgtcgcgcg ttcggcgttg tcgccgcaag tcaacggcaa cctgtcggtc cagcgccaga agtggtccga caatgtctac tacggccccg gcccgctcgc gggcgagcaa tcgtggaaca acaccggcac gctcggcctg tcgtatcacc tcgatatctg gggcaaggac aggaacgccg ccgagcgcgc gctcgatgcc gcccacgcga gcgcggcgga cgcccgcgcc gcccagctcg aactggaagg caacgtggtg cgcacataca tcgagatgtc gatgaactac gcgctgctcg acatcgccaa gtccaccttg cagcagcagc aacagatcgt ggatctggcc agccgacgcc tgaaaggcgg catcggcacg caactggaag tgagccaggc cgaaacgccg ctgccggaat acgaacgcca gatcgacgag atcgaagaaa agatcgcgct tggccgcaat cagctggccg cgctggccgg caaaggtccg ggcgcgggcg attcgatcca gcgcccgacg ctttcgctga acacgccggc cgggctgccg agcgcattgc ccgcggacct gatcggccat cggccggacg tggtggcggc gcgctggtcg gtcgcggcgc aggcgcgcgg tatcgacgtc gccaaggcgg acttctatcc ggacatcaac ctgctggcgt cgatcggcgg atatgcggca atggggccgc tgttccagtt cctgaagaat ccgtcgcaca gctggagcgg cgggccggcg ctgtcgctgc cgatcctcga cggcggccgc ctgcgttcgc agctcggcgc ggcttcggcg ggctatgacg aagccgtcga ccgttacaac cagtcgatcg tcggcgcgct caaggacatt tccgatcagg tgatccggat ccgctcgctt gcgacccagg ccgaggacgc cgaccgttcg gtcgcgacgg cccgcaagaa ctacgatctg tcgcgcgaag gttaccggcg cgggctgacc gactatctga acgtgctgat cgcgcaaaac cagttgctgc acgcgcagga aggcgtggcg aagattcagg ccgaacggct cggcgcgcat gcgtcgctgg tcacggcact gggcggcgga ctcgacgacc cggccaacgg tccgctggcg aacgagacgc tgccggcgca cggcaagggt aagacggtgg cgcccggttc gaccgccagc gccgagccgg ccaggtcagg aagctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BuxeA.01673.o.A2.PS01372

PURIFICATION DATe:	2/2/2012
CONCENTRATION:	23.8mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	81

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTGTGCCAGT ACCGGAGGCG TCGCGCCGCA AGCGAGCGGA GTCCAGCCCG CTTCGCTCGA CGCCGGCAAC GCGATCCGCG CCGCCAATGC GGACGCCCAA TGGCCGTCCG TCGACTGGTG GCGCGCCTAC AACGATCCGC AATTGAACGA GTGGATCGAC GCGGCACAGG CCGGCAATCC GAGCCTCGCG GCCGCCCAGG CACGCGTGCG CGAAGCCATG TCGATGGCGG GCGTCGCGCG TTCGGCGTTG TCGCCGCAAG TCAACGGCAA CCTGTCGGTC CAGCGCCAGA AGTGGTCCGA CAATGTCTAC TACGGCCCCG GCCCGCTCGC GGGCGAGCAA TCGTGGAACA ACACCGGCAC GCTCGGCCTG TCGTATCACC TCGATATCTG GGGCAAGGAC AGGAACGCCG CCGAGCGCGC GCTCGATGCC GCCCACGCGA GCGCGGCGGA CGCCCGCGCC GCCCAGCTCG AACTGGAAGG CAACGTGGTG CGCACATACA TCGAGATGTC GATGAACTAC GCGCTGCTCG ACATCGCCAA GTCCACCTTG CAGCAGCAGC AACAGATCGT GGATCTGGCC AGCCGACGCC TGAAAGGCGG CATCGGCACG CAACTGGAAG TGAGCCAGGC CGAAACGCCG CTGCCGGAAT ACGAACGCCA GATCGACGAG ATCGAAGAAA AGATCGCGCT TGGCCGCAAT CAGCTGGCCG CGCTGGCCGG CAAAGGTCCG GGCGCGGGCG ATTCGATCCA GCGCCCGACG CTTTCGCTGA ACACGCCGGC CGGGCTGCCG AGCGCATTGC CCGCGGACCT GATCGGCCAT CGGCCGGACG TGGTGGCGGC GCGCTGGTCG GTCGCGGCGC AGGCGCGCGG TATCGACGTC GCCAAGGCGG ACTTCTATCC GGACATCAAC CTGCTGGCGT CGATCGGCGG ATATGCGGCA ATGGGGCCGC TGTTCCAGTT CCTGAAGAAT CCGTCGCACA GCTGGAGCGG CGGGCCGGCG CTGTCGCTGC CGATCCTCGA CGGCGGCCGC CTGCGTTCGC AGCTCGGCGC GGCTTCGGCG GGCTATGACG AAGCCGTCGA CCGTTACAAC CAGTCGATCG TCGGCGCGCT CAAGGACATT TCCGATCAGG TGATCCGGAT CCGCTCGCTT GCGACCCAGG CCGAGGACGC CGACCGTTCG GTCGCGACGG CCCGCAAGAA CTACGATCTG TCGCGCGAAG GTTACCGGCG CGGGCTGACC GACTATCTGA ACGTGCTGAT CGCGCAAAAC CAGTTGCTGC ACGCGCAGGA AGGCGTGGCG AAGATTCAGG CCGAACGGCT CGGCGCGCAT GCGTCGCTGG TCACGGCACT GGGCGGCGGA CTCGACGACC CGGCCAACGG TCCGCTGGCG AACGAGACGC TGCCGGCGCA CGGCAAGGGT AAGACGGTGG CGCCCGGTTC GACCGCCAGC GCCGAGCCGG CCAGGTCAGG AAGCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA