BuxeA.01673.n

MFS efflux system, outer membrane porin

CENTER ID: BuxeA.01673.n
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.n.A1.GE32004 Full length( BuxeA.01673.n ) 1 511
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.3422
RefSeq: YP_559845.1
UniProt: Q13VU6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSQPLLSQL LRQAGTALCT ISLVGCAVGP DFVKPDDRLA AVQLSPRADY AQAQETSAAN FPSSWWTLFN DDVLADLQAR AQAGNLNLQM ASERIEQSRA QLGIASSLLL PSVGASAGYS REAISEHGKF AALGAPTSAS DFWQLGFDAS WELDLWGRAR RSRESAAAAF EATVYEREAA RVALSAEVAR TYLQLRGTQA QLDITRQNLE VADRTLRLAE SRERNGVATR FETASARAQL ATVEALVPEL VQRRNTLMNV LALLLGEQPR ALDAQLLEAM PLPSLPSNVP VGLPSELAHR RPDIQRAEAQ LHAATAAIGV AKADFYPRIG LKGRVGVEAF EFNDLDSWDS RFFSVGPTVY LPIFQGGRLK QRLALNEARQ RTAAIGYRQT VLQAWHEVDN ALDAWAAQQN RHAELVVSYE QNQQALHAAE RGYQEGAADY LNVLAAQRGV LASQTSLNAS ATNAALTLVN LYKSLGGGWD PGAFSSEQQT GGDASLTTAV SSATSQSKVD Q
NT Sequence
ATGACGTCAC AACCGCTGCT CTCACAACTG CTGCGACAGG CAGGCACGGC ACTCTGCACG ATCTCCCTGG TCGGTTGCGC GGTTGGTCCT GACTTCGTGA AACCCGACGA CAGGCTCGCA GCCGTTCAAC TGTCGCCCAG GGCGGACTAC GCGCAGGCTC AGGAGACATC CGCCGCGAAC TTCCCGTCTT CATGGTGGAC GTTGTTCAAC GATGACGTAC TGGCGGATCT GCAAGCCCGT GCGCAGGCGG GCAACCTGAA CCTGCAGATG GCTTCCGAGC GCATCGAGCA AAGTCGGGCG CAGTTGGGGA TTGCCTCCTC GCTGCTGCTG CCCAGTGTGG GTGCTTCGGC GGGCTATTCC CGCGAGGCCA TCAGCGAGCA CGGCAAGTTT GCCGCGTTGG GTGCTCCGAC GAGCGCGAGC GACTTCTGGC AGTTGGGATT CGACGCCAGT TGGGAACTCG ACCTATGGGG CCGCGCGCGT CGATCGCGCG AAAGCGCCGC GGCCGCCTTC GAGGCCACGG TGTATGAGCG CGAGGCGGCA CGCGTGGCCC TGTCCGCCGA GGTGGCCCGT ACCTATCTCC AATTGCGCGG CACGCAAGCG CAACTGGACA TCACCCGGCA GAACCTGGAG GTTGCCGATC GCACGCTCCG CCTTGCCGAA AGCCGTGAGC GCAATGGCGT CGCCACACGT TTTGAAACCG CTTCGGCGCG CGCGCAACTG GCAACGGTCG AGGCGCTGGT ACCCGAATTG GTCCAGCGCC GCAACACGCT GATGAACGTC CTGGCATTGC TGCTGGGAGA GCAGCCGCGC GCCCTCGATG CGCAGTTGCT TGAAGCGATG CCTCTGCCGT CGCTTCCTTC CAATGTGCCG GTGGGCCTGC CCTCCGAATT GGCGCACAGG CGGCCGGACA TACAGCGCGC CGAAGCCCAG CTCCATGCTG CGACGGCAGC CATCGGCGTC GCCAAAGCCG ATTTCTACCC GCGAATCGGC CTCAAAGGCA GGGTCGGCGT CGAAGCCTTC GAGTTCAACG ACCTCGACAG TTGGGATTCC CGGTTCTTCT CCGTCGGCCC CACGGTCTAT CTTCCCATCT TCCAGGGCGG GCGTCTGAAG CAGCGCCTGG CGCTGAACGA GGCACGGCAA AGAACTGCCG CCATCGGCTA CCGGCAGACG GTGCTGCAAG CCTGGCATGA AGTGGACAAC GCCCTGGACG CCTGGGCAGC CCAGCAGAAC CGCCATGCCG AGTTGGTGGT TTCGTACGAG CAGAACCAGC AGGCGCTGCA TGCTGCCGAG CGCGGCTATC AGGAAGGCGC TGCCGACTAC CTGAACGTCC TGGCCGCACA GCGCGGTGTC CTCGCAAGCC AGACCAGCCT CAATGCCAGC GCGACCAACG CCGCGCTCAC CCTGGTCAAT CTCTACAAAT CCCTGGGCGG TGGCTGGGAC CCCGGCGCAT TCTCTTCCGA GCAGCAGACC GGCGGCGACG CGTCGCTGAC GACCGCTGTG TCGTCGGCCA CCTCGCAATC GAAGGTTGAC CAA
Details for BuxeA.01673.n.A1.GE32004
HARVESTED ON: 4/5/2011
SEQUENCED ON: 4/11/2011
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 46
PERCENT COVERAGE: 26
Validated AA Sequence
MASERIEQSR AQLGIASSLL LPSVGASAGY SREAISEHGS LPRWVLRXAR ATSGSWDSTP VGNSTYGXAR VDXXKAPRPP SRPRCMXXRR HAWPCPPRWP VPISNCAARK RNWTSPGRTW XXXIARSALX KAXSAMASPH V
Validated NT Sequence
gccatgtttn nnnnnctccn nnnnnnannn nnannnaann anaannnggg tnccctggna gctcnnaccc ngggtcctgg ttcgatgacg tcacaaccgc tgatcnntca ctagcanaca ggcaggnacg gnnctctgca cganctccct ggtcngntgn ncgggtcctg acttcntgaa accnnannac nggctgncnn nnnnncnnct gtcgcnnnag ncggactacg cgcaggctca ggagacatcc gccgcgaact tcccgtcttc atggtggacg ttgttcaacg atgacgtact ggcggatctg caagcccgtg cgcaggcggg caacctgaac ctgcagatgg cttccgagcg catcgagcaa agtcgggcgc agttggggat tgcctcctcg ctgctgctgc ccagtgtggg tgcttcggcg ggctattccc gcgaggccat cagcgagcac ggaagtttgc cgcgttgggt gctccgacna gcgcgagcga cttctggcag ttgggattcg acgccagttg ggaactcgac ctatggggnc gcgcgcgtcg atcncncgaa agcgccgcgg ccgccttcna ggccacggtg tatgagnnng aggcggcacg cgtggccctg tccgccgagg tggcccgtac ctatctccaa ttgcgcggca cgcaagcgca actggacatc acccggcaga acctggnngn tgncgatcgc acgctccgcc ttgncgaaag ccntgagcgc aatggcgtcg ccacacgtnt gaaancgctn cggcgcgcgc ncnnnnggga acggtcgagg cgctggtann ngaattggnn cancgccgca acacgctgnn nnangtcctg gcattgctgc tgggannagn agccgcgnnc ccncnatnnn nnntnntnga ncnnngctnt nnncgtngnt t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTSQPLLSQ LLRQAGTALC TISLVGCAVG PDFVKPDDRL AAVQLSPRAD YAQAQETSAA NFPSSWWTLF NDDVLADLQA RAQAGNLNLQ MASERIEQSR AQLGIASSLL LPSVGASAGY SREAISEHGK FAALGAPTSA SDFWQLGFDA SWELDLWGRA RRSRESAAAA FEATVYEREA ARVALSAEVA RTYLQLRGTQ AQLDITRQNL EVADRTLRLA ESRERNGVAT RFETASARAQ LATVEALVPE LVQRRNTLMN VLALLLGEQP RALDAQLLEA MPLPSLPSNV PVGLPSELAH RRPDIQRAEA QLHAATAAIG VAKADFYPRI GLKGRVGVEA FEFNDLDSWD SRFFSVGPTV YLPIFQGGRL KQRLALNEAR QRTAAIGYRQ TVLQAWHEVD NALDAWAAQQ NRHAELVVSY EQNQQALHAA ERGYQEGAAD YLNVLAAQRG VLASQTSLNA SATNAALTLV NLYKSLGGGW DPGAFSSEQQ TGGDASLTTA VSSATSQSKV DQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgtcacaa ccgctgctct cacaactgct gcgacaggca ggcacggcac tctgcacgat ctccctggtc ggttgcgcgg ttggtcctga cttcgtgaaa cccgacgaca ggctcgcagc cgttcaactg tcgcccaggg cggactacgc gcaggctcag gagacatccg ccgcgaactt cccgtcttca tggtggacgt tgttcaacga tgacgtactg gcggatctgc aagcccgtgc gcaggcgggc aacctgaacc tgcagatggc ttccgagcgc atcgagcaaa gtcgggcgca gttggggatt gcctcctcgc tgctgctgcc cagtgtgggt gcttcggcgg gctattcccg cgaggccatc agcgagcacg gcaagtttgc cgcgttgggt gctccgacga gcgcgagcga cttctggcag ttgggattcg acgccagttg ggaactcgac ctatggggcc gcgcgcgtcg atcgcgcgaa agcgccgcgg ccgccttcga ggccacggtg tatgagcgcg aggcggcacg cgtggccctg tccgccgagg tggcccgtac ctatctccaa ttgcgcggca cgcaagcgca actggacatc acccggcaga acctggaggt tgccgatcgc acgctccgcc ttgccgaaag ccgtgagcgc aatggcgtcg ccacacgttt tgaaaccgct tcggcgcgcg cgcaactggc aacggtcgag gcgctggtac ccgaattggt ccagcgccgc aacacgctga tgaacgtcct ggcattgctg ctgggagagc agccgcgcgc cctcgatgcg cagttgcttg aagcgatgcc tctgccgtcg cttccttcca atgtgccggt gggcctgccc tccgaattgg cgcacaggcg gccggacata cagcgcgccg aagcccagct ccatgctgcg acggcagcca tcggcgtcgc caaagccgat ttctacccgc gaatcggcct caaaggcagg gtcggcgtcg aagccttcga gttcaacgac ctcgacagtt gggattcccg gttcttctcc gtcggcccca cggtctatct tcccatcttc cagggcgggc gtctgaagca gcgcctggcg ctgaacgagg cacggcaaag aactgccgcc atcggctacc ggcagacggt gctgcaagcc tggcatgaag tggacaacgc cctggacgcc tgggcagccc agcagaaccg ccatgccgag ttggtggttt cgtacgagca gaaccagcag gcgctgcatg ctgccgagcg cggctatcag gaaggcgctg ccgactacct gaacgtcctg gccgcacagc gcggtgtcct cgcaagccag accagcctca atgccagcgc gaccaacgcc gcgctcaccc tggtcaatct ctacaaatcc ctgggcggtg gctgggaccc cggcgcattc tcttccgagc agcagaccgg cggcgacgcg tcgctgacga ccgctgtgtc gtcggccacc tcgcaatcga aggttgacca ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa