BuxeA.01673.l

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuxeA.01673.l
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.l.A2.GE34894 Mature protein( BuxeA.01673.l ) 31 523
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.4629
RefSeq: YP_560995.1
UniProt: Q13SJ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQSDRISRAR TLAPRALTIA VAAALAGLLS ACVVGPDYKR PAATIPASYK EAAPGWKVAE PADQHDRGDW WTIYEDPQLN ALEDRLNTAN QTVAQFAAAY RQARALVGEA RAAYFPTIGA SAGATRSGNG SSSSRSSTAT TSRSGISNSF NVQLDASWEP DLWGSVTRSV NSQKAGQQSA AADLANARLS AQATLAQTYF SLRALDSTQK LLDDTVAAYQ RSLQLTQNQY AAGVAARSDV IQAQTQLQSA QAAAIDNGVQ RAQDEHAIAV LVGEPASTFS IPPMPLTATP PAVPAQMPSA LLERRPDIAS AERKAAAANE QIGVAIAAFF PSLTLSASGG FENSVFSQLL TAPSRFWTLG PQLAATLFDA GLRQAQTDAA RAAYDGDVAS YRQTVLAAFQ DVEDNLASQR ILEQEIVVQR QAVDSARQAL AIVTNEYKAG TVGYVNVLTA QTTAFTAEQK LENIAGQRMV SSVGLVKALG GGWDASQMNR ETGDVAAPAP LPASAAAPVA QNGATPPSQM HNE
NT Sequence
ATGCAGTCTG ATCGAATCTC GCGCGCGCGG ACGCTTGCGC CACGCGCCCT GACTATTGCT GTCGCAGCGG CTCTCGCCGG CCTGCTGTCC GCCTGCGTGG TCGGCCCCGA CTACAAGCGG CCCGCGGCCA CGATCCCGGC GTCATACAAG GAAGCTGCCC CCGGCTGGAA AGTCGCCGAG CCGGCCGATC AGCACGATCG AGGCGACTGG TGGACCATTT ACGAAGATCC GCAACTCAAC GCGCTGGAAG ACCGGCTGAA CACCGCGAAC CAGACCGTCG CCCAGTTCGC CGCCGCGTAC CGTCAGGCGC GCGCGCTGGT TGGCGAAGCG CGTGCAGCGT ATTTCCCCAC CATCGGCGCA TCGGCGGGCG CCACGCGTTC CGGCAACGGC TCGTCGTCGA GTCGCAGCTC GACTGCCACG ACGAGCCGCT CCGGCATCAG CAATAGTTTC AACGTACAAC TCGACGCAAG CTGGGAGCCG GACCTGTGGG GTTCGGTCAC CCGCTCGGTC AACTCGCAGA AGGCCGGCCA GCAAAGCGCC GCGGCGGACC TCGCGAACGC GCGGCTGTCG GCGCAAGCCA CGCTTGCCCA GACCTATTTC TCGCTGCGCG CGCTCGATTC GACTCAGAAA TTACTCGACG ACACCGTCGC GGCTTACCAA CGCTCGCTGC AACTCACGCA GAACCAGTAC GCGGCCGGCG TCGCCGCGCG CTCCGACGTG ATCCAGGCGC AAACGCAATT GCAGTCGGCC CAGGCAGCCG CGATCGACAA CGGCGTCCAG CGCGCCCAGG ACGAGCACGC GATCGCCGTA CTGGTCGGCG AACCCGCCTC CACCTTCTCG ATTCCGCCGA TGCCGCTAAC GGCCACGCCG CCCGCCGTGC CGGCGCAAAT GCCGTCGGCG CTGCTCGAAC GGCGGCCGGA CATCGCGTCG GCGGAACGCA AGGCCGCCGC GGCTAACGAA CAGATCGGCG TAGCCATCGC CGCTTTTTTC CCGTCGCTGA CCCTGTCCGC TTCCGGCGGC TTCGAAAATT CAGTGTTCTC GCAATTGCTG ACCGCGCCGT CGCGCTTCTG GACGCTCGGC CCGCAACTCG CCGCAACGCT CTTCGACGCC GGTCTGCGCC AGGCCCAGAC CGACGCCGCC CGAGCCGCCT ACGACGGCGA CGTCGCGAGC TACCGTCAAA CGGTGCTGGC CGCATTCCAG GACGTCGAGG ACAATCTCGC ATCACAGCGC ATCCTCGAGC AGGAAATCGT CGTGCAGCGG CAAGCGGTGG ATTCGGCGCG TCAGGCGCTG GCCATCGTCA CGAACGAGTA CAAGGCGGGC ACGGTCGGCT ACGTCAACGT GCTAACCGCG CAAACCACCG CCTTCACGGC TGAGCAGAAA CTGGAGAACA TCGCCGGGCA GCGGATGGTG TCGTCGGTAG GTCTGGTGAA GGCGCTGGGC GGCGGCTGGG ACGCGTCGCA GATGAATCGC GAAACGGGCG ATGTGGCCGC GCCAGCGCCG CTGCCGGCAT CAGCGGCTGC GCCGGTCGCG CAAAACGGGG CGACGCCGCC TTCGCAGATG CACAACGAA
Details for BuxeA.01673.l.A2.GE34894
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 54kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 93
PERCENT COVERAGE: 65
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMACVVGPDY KRPAATIPAS YKEAAPGWKV AEPADQHDRG DWWTIYEDPQ LNALEDRLNT ANQTVAQFAA AYRQARALVG EARAAYFPTI GASAGATRSG NGSSSSRSST ATTSRSGISN SFNVQLDASW EPDLWGSVTR SVNSQKAGQQ SAAADLANAR LSAQATLAQT YFSLRALDST QKLLDDTVAA YQRSLQLTQN QYAAGVAARS DVIQAQTQLQ SAQAAAIDNG VQRAQDEHAI AVLXGEPAST FSIPPMPLTA TPPAVPAQMX SALLERRPDI ASXERKAAXA NEQIGVXIAL FXVAXPVXXX XFXNXCXXX
Validated NT Sequence
tttgnnnnnt ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcctgc gtggtcggcc ccgactacaa gcggcccgcg gccacgatcc cggcgtcata caaggaagct gcccccggct ggaaagtcgc cgagccggcc gatcagcacg atcgaggcga ctggtggacc atttacgaag atccgcaact caacgcgctg gaagaccggc tgaacaccgc gaaccagacc gtcgcccagt tcgccgccgc gtaccgtcag gcgcgcgcgc tggttggcga agcgcgtgca gcgtatttcc ccaccatcgg cgcatcggcg ggcgccacgc gttccggcaa cggctcgtcg tcgagtcgca gctcgactgc cacgacgagc cgctccggca tcagcaatag tttcaacgta caactcgacg caagctggga gccggacctg tggggttcgg tcacccgctc ggtcaactcg cagaaggccg gccagcaaag cgccgcggcg gacctcgcga acgcgcggct gtcggcgcaa gccacgcttg cccagaccta tttctcgctg cgcgcgctcg attcgactca gaaattactc gacgacaccg tcgcggctta ccaacgctcg ctgcaactca cgcagaacca gtacgcggcc ggcgtcgccg cgcgctccga cgtgatccag gcgcaaacgc aattgcagtc ggcccaggca gccgcgatcg acaacggcgt ccagcgcgcc caggacgagc acgcgatcgc cgtactgntc ggcgaacccg cctccacctt ctcgattccg ccgatgccgc taacggccac gccgcccgcc gtgccggcgc aaatgncgtc ggcgctgctc gaacggcggc cggacatcgc gtcgnnggaa cgcaaggccg ccncggctaa cgaacagatc ggcgtancca tcgcnctttt tnccgtcgct gancctgtcn nttncgnnng cttcnaaaat cantgtnnnn nannntgacn nncnncnnnn nttctngang ntngnnnnan nnnntttnna nnnnnnnnnn ncnnnnnaan nnnncnnccn nnnncnnnnn nnnnnntnnn ngnnncnnnn annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gnngnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMACVVGPDY KRPAATIPAS YKEAAPGWKV AEPADQHDRG DWWTIYEDPQ LNALEDRLNT ANQTVAQFAA AYRQARALVG EARAAYFPTI GASAGATRSG NGSSSSRSST ATTSRSGISN SFNVQLDASW EPDLWGSVTR SVNSQKAGQQ SAAADLANAR LSAQATLAQT YFSLRALDST QKLLDDTVAA YQRSLQLTQN QYAAGVAARS DVIQAQTQLQ SAQAAAIDNG VQRAQDEHAI AVLVGEPAST FSIPPMPLTA TPPAVPAQMP SALLERRPDI ASAERKAAAA NEQIGVAIAA FFPSLTLSAS GGFENSVFSQ LLTAPSRFWT LGPQLAATLF DAGLRQAQTD AARAAYDGDV ASYRQTVLAA FQDVEDNLAS QRILEQEIVV QRQAVDSARQ ALAIVTNEYK AGTVGYVNVL TAQTTAFTAE QKLENIAGQR MVSSVGLVKA LGGGWDASQM NRETGDVAAP APLPASAAAP VAQNGATPPS QMHNE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcctgcgtg gtcggccccg actacaagcg gcccgcggcc acgatcccgg cgtcatacaa ggaagctgcc cccggctgga aagtcgccga gccggccgat cagcacgatc gaggcgactg gtggaccatt tacgaagatc cgcaactcaa cgcgctggaa gaccggctga acaccgcgaa ccagaccgtc gcccagttcg ccgccgcgta ccgtcaggcg cgcgcgctgg ttggcgaagc gcgtgcagcg tatttcccca ccatcggcgc atcggcgggc gccacgcgtt ccggcaacgg ctcgtcgtcg agtcgcagct cgactgccac gacgagccgc tccggcatca gcaatagttt caacgtacaa ctcgacgcaa gctgggagcc ggacctgtgg ggttcggtca cccgctcggt caactcgcag aaggccggcc agcaaagcgc cgcggcggac ctcgcgaacg cgcggctgtc ggcgcaagcc acgcttgccc agacctattt ctcgctgcgc gcgctcgatt cgactcagaa attactcgac gacaccgtcg cggcttacca acgctcgctg caactcacgc agaaccagta cgcggccggc gtcgccgcgc gctccgacgt gatccaggcg caaacgcaat tgcagtcggc ccaggcagcc gcgatcgaca acggcgtcca gcgcgcccag gacgagcacg cgatcgccgt actggtcggc gaacccgcct ccaccttctc gattccgccg atgccgctaa cggccacgcc gcccgccgtg ccggcgcaaa tgccgtcggc gctgctcgaa cggcggccgg acatcgcgtc ggcggaacgc aaggccgccg cggctaacga acagatcggc gtagccatcg ccgctttttt cccgtcgctg accctgtccg cttccggcgg cttcgaaaat tcagtgttct cgcaattgct gaccgcgccg tcgcgcttct ggacgctcgg cccgcaactc gccgcaacgc tcttcgacgc cggtctgcgc caggcccaga ccgacgccgc ccgagccgcc tacgacggcg acgtcgcgag ctaccgtcaa acggtgctgg ccgcattcca ggacgtcgag gacaatctcg catcacagcg catcctcgag caggaaatcg tcgtgcagcg gcaagcggtg gattcggcgc gtcaggcgct ggccatcgtc acgaacgagt acaaggcggg cacggtcggc tacgtcaacg tgctaaccgc gcaaaccacc gccttcacgg ctgagcagaa actggagaac atcgccgggc agcggatggt gtcgtcggta ggtctggtga aggcgctggg cggcggctgg gacgcgtcgc agatgaatcg cgaaacgggc gatgtggccg cgccagcgcc gctgccggca tcagcggctg cgccggtcgc gcaaaacggg gcgacgccgc cttcgcagat gcacaacgaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa