BuxeA.01673.k

Aromatic acid efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuxeA.01673.k
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.k.A2.GE34893 Mature protein( BuxeA.01673.k ) 21 510
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.5086
RefSeq: YP_552728.1
UniProt: Q13RB1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLSRALPLL PLLPLLPLAA ALDGCMNVGP NYALPQQALV NAPLANTPIE GADAALTTRR NVPPVWWKLY DDPVLNGLVD EALQSNTDLR VAAANLARSR EALGVAQAQG GFSGKTSALI ERAQESAEQY LLTEKLPVAN EGDIGISVSY EIDLFGKLRR GVEAAQADSE SVQAAADLAR ITVVADVVRS YVEQCSAADE LRIAQQSLSL QKQRVDVSRR LRDAGRGNQP DVTRGQTQVD TLSADIPRYT ARRKIAQYRL AALLARAPSD LPPAVLACDT LPQIRQPIPI GDGAALLKRR PDVREAERQL AASTARIGVA TGALYPTVSI GASAGLTGIL EDLGTSPTAR WGFGPLISWT FPANGARGRV GEAQASSQVA LAKFDGVVLT ALRETETSLA TYASDYARAD ALRAAVKSAA ASADETHRLY LAGRESFISD LDATRTLTST KSQAAAAEGQ VAIDQVNLFL ALGGGWEMDP PNASGSPAPA AAAMPVKAAP TAAAASAKAP
NT Sequence
ATGAAGCTGT CGCGCGCCCT GCCCTTATTG CCGCTGCTGC CACTATTACC GCTGGCCGCC GCGCTCGACG GCTGCATGAA CGTCGGCCCC AACTATGCGT TGCCGCAACA GGCGCTCGTC AACGCGCCGC TAGCCAATAC GCCGATCGAA GGCGCCGACG CGGCCCTGAC CACGCGTCGC AACGTGCCGC CCGTATGGTG GAAGCTGTAC GACGATCCGG TGCTGAACGG ACTCGTCGAC GAGGCGCTGC AATCGAACAC CGACTTGCGC GTCGCGGCGG CGAACCTCGC GCGCTCGCGC GAAGCGCTCG GCGTCGCGCA GGCGCAAGGT GGTTTCTCCG GCAAGACTTC GGCGCTGATC GAACGCGCGC AGGAATCCGC CGAGCAATAC CTGCTCACCG AGAAACTGCC GGTCGCCAAT GAAGGCGACA TCGGCATCAG CGTCTCGTAT GAAATCGATC TGTTCGGCAA GCTGCGGCGC GGCGTGGAAG CGGCGCAGGC AGACAGCGAG TCCGTGCAGG CCGCGGCCGA TCTCGCGCGG ATCACCGTGG TGGCGGACGT GGTGCGCTCA TACGTCGAGC AATGTTCGGC AGCCGATGAG CTCAGAATCG CGCAACAGTC GCTCTCCCTG CAGAAGCAGC GCGTGGACGT CTCGCGCCGC CTGCGTGACG CGGGCCGCGG TAATCAGCCC GACGTGACGC GCGGGCAGAC CCAGGTCGAT ACGCTGTCGG CGGATATTCC GCGCTATACG GCACGCCGCA AGATTGCCCA GTACCGGCTC GCCGCGCTGC TCGCCCGCGC GCCATCCGAC TTGCCGCCGG CCGTGCTCGC GTGCGACACG CTGCCGCAAA TCCGCCAGCC GATTCCGATC GGCGACGGCG CCGCGCTGCT CAAGCGCCGC CCGGATGTGC GCGAAGCCGA GCGTCAACTG GCGGCGTCCA CGGCGCGCAT TGGCGTGGCC ACCGGGGCGC TGTATCCGAC GGTGAGCATC GGCGCCTCGG CCGGCTTGAC CGGGATCCTC GAAGACCTGG GCACCTCGCC CACGGCGCGC TGGGGCTTCG GCCCGCTGAT CAGCTGGACC TTCCCGGCGA ACGGCGCGCG TGGCCGGGTG GGCGAGGCCC AGGCGTCGAG CCAGGTGGCT CTGGCGAAGT TCGACGGCGT GGTGCTGACC GCGTTGCGCG AGACCGAAAC CAGTCTGGCA ACTTATGCGT CGGACTACGC CCGCGCCGAC GCTTTGCGCG CCGCCGTGAA GTCGGCAGCC GCGTCCGCGG ATGAAACGCA TCGGCTGTAT CTCGCGGGGC GTGAGTCGTT TATTTCGGAT CTGGATGCGA CGCGCACGCT CACTTCGACG AAATCTCAGG CGGCCGCCGC GGAAGGCCAG GTGGCGATCG ATCAGGTCAA CCTGTTCCTC GCGCTGGGCG GCGGGTGGGA AATGGATCCG CCGAATGCGT CCGGCAGTCC GGCGCCCGCA GCCGCCGCAA TGCCGGTCAA AGCGGCGCCG ACCGCCGCAG CGGCTTCGGC AAAAGCGCCT
Details for BuxeA.01673.k.A2.GE34893
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 53kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 74
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMALDGCMNV GPNYALPQQA LVNAPLANTP IEGADAALTT RRNVPPVWWK LYDDPVLNGL VDEALQSNTD LRVAAANLAR SREALGVAQA QGGFSGKTSA LIERAQESAE QYLLTEKLPV ANEGDIGISV SYEIDLFGKL RRGVEAAQAD SESVQAAADL ARITVVADVV RSYVEQCSAA DELRIAQQSL SLQKQRVDVS RRLRDAGRGN QPDVTRGQTQ VDTLSADIPR YTARRKIAQY RLAALLARAP SDLPPAVLAC DTLPQIRQPI PIGXXXALLX RRPDVXEAXR QLAASTGAXX XXXAXXPXXX IGXRXXTXXX XXWAXXXGXL GXXXDXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
ttnnttnnct ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcgctc gacggctgca tgaacgtcgg ccccaactat gcgttgccgc aacaggcgct cgtcaacgcg ccgctagcca atacgccgat cgaaggcgcc gacgcggccc tgaccacgcg tcgcaacgtg ccgcccgtat ggtggaagct gtacgacgat ccggtgctga acggactcgt cgacgaggcg ctgcaatcga acaccgactt gcgcgtcgcg gcggcgaacc tcgcgcgctc gcgcgaagcg ctcggcgtcg cgcaggcgca aggtggtttc tccggcaaga cttcggcgct gatcgaacgc gcgcaggaat ccgccgagca atacctgctc accgagaaac tgccggtcgc caatgaaggc gacatcggca tcagcgtctc gtatgaaatc gatctgttcg gcaagctgcg gcgcggcgtg gaagcggcgc aggcagacag cgagtccgtg caggccgcgg ccgatctcgc gcggatcacc gtggtggcgg acgtggtgcg ctcatacgtc gagcaatgtt cggcagccga tgagctcaga atcgcgcaac agtcgctctc cctgcagaag cagcgcgtgg acgtctcgcg ccgcctgcgt gacgcgggcc gcggtaatca gcccgacgtg acgcgcgggc agacccaggt cgatacgctg tcggcggata ttccgcgcta tacggcacgc cgcaagattg cccagtaccg gctcgccgcg ctgctcgccc gcgcgccatc cgacttgccg ccggccgtgc tcgcgtgcga cacgctgccg caaatccgcc agccgattcc natcggcgan gnnnccgcgc tgctcnagcg ccgcccggat gtgcncgaag ccnancgtca actggcggcg tccacgggcg cattnnntnn nnnnngggcn ntnnatccga ngnngannat cggnnctcgn cnnntnaccn gnnncntcna nanntgggcn nctnnncnng gnnngctggg cttnnnnnct gatcancngn nnnnnnnnnn gnnnnnnnng nnnnncngnn nnnnnngnnn nnnnnnnnnn nnntnacnnn nnngnnnncn naa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMALDGCMNV GPNYALPQQA LVNAPLANTP IEGADAALTT RRNVPPVWWK LYDDPVLNGL VDEALQSNTD LRVAAANLAR SREALGVAQA QGGFSGKTSA LIERAQESAE QYLLTEKLPV ANEGDIGISV SYEIDLFGKL RRGVEAAQAD SESVQAAADL ARITVVADVV RSYVEQCSAA DELRIAQQSL SLQKQRVDVS RRLRDAGRGN QPDVTRGQTQ VDTLSADIPR YTARRKIAQY RLAALLARAP SDLPPAVLAC DTLPQIRQPI PIGDGAALLK RRPDVREAER QLAASTARIG VATGALYPTV SIGASAGLTG ILEDLGTSPT ARWGFGPLIS WTFPANGARG RVGEAQASSQ VALAKFDGVV LTALRETETS LATYASDYAR ADALRAAVKS AAASADETHR LYLAGRESFI SDLDATRTLT STKSQAAAAE GQVAIDQVNL FLALGGGWEM DPPNASGSPA PAAAAMPVKA APTAAAASAK AP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgctcgac ggctgcatga acgtcggccc caactatgcg ttgccgcaac aggcgctcgt caacgcgccg ctagccaata cgccgatcga aggcgccgac gcggccctga ccacgcgtcg caacgtgccg cccgtatggt ggaagctgta cgacgatccg gtgctgaacg gactcgtcga cgaggcgctg caatcgaaca ccgacttgcg cgtcgcggcg gcgaacctcg cgcgctcgcg cgaagcgctc ggcgtcgcgc aggcgcaagg tggtttctcc ggcaagactt cggcgctgat cgaacgcgcg caggaatccg ccgagcaata cctgctcacc gagaaactgc cggtcgccaa tgaaggcgac atcggcatca gcgtctcgta tgaaatcgat ctgttcggca agctgcggcg cggcgtggaa gcggcgcagg cagacagcga gtccgtgcag gccgcggccg atctcgcgcg gatcaccgtg gtggcggacg tggtgcgctc atacgtcgag caatgttcgg cagccgatga gctcagaatc gcgcaacagt cgctctccct gcagaagcag cgcgtggacg tctcgcgccg cctgcgtgac gcgggccgcg gtaatcagcc cgacgtgacg cgcgggcaga cccaggtcga tacgctgtcg gcggatattc cgcgctatac ggcacgccgc aagattgccc agtaccggct cgccgcgctg ctcgcccgcg cgccatccga cttgccgccg gccgtgctcg cgtgcgacac gctgccgcaa atccgccagc cgattccgat cggcgacggc gccgcgctgc tcaagcgccg cccggatgtg cgcgaagccg agcgtcaact ggcggcgtcc acggcgcgca ttggcgtggc caccggggcg ctgtatccga cggtgagcat cggcgcctcg gccggcttga ccgggatcct cgaagacctg ggcacctcgc ccacggcgcg ctggggcttc ggcccgctga tcagctggac cttcccggcg aacggcgcgc gtggccgggt gggcgaggcc caggcgtcga gccaggtggc tctggcgaag ttcgacggcg tggtgctgac cgcgttgcgc gagaccgaaa ccagtctggc aacttatgcg tcggactacg cccgcgccga cgctttgcgc gccgccgtga agtcggcagc cgcgtccgcg gatgaaacgc atcggctgta tctcgcgggg cgtgagtcgt ttatttcgga tctggatgcg acgcgcacgc tcacttcgac gaaatctcag gcggccgccg cggaaggcca ggtggcgatc gatcaggtca acctgttcct cgcgctgggc ggcgggtggg aaatggatcc gccgaatgcg tccggcagtc cggcgcccgc agccgccgca atgccggtca aagcggcgcc gaccgccgca gcggcttcgg caaaagcgcc ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa