BuxeA.01673.j

ABC efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuxeA.01673.j
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.j.A1.GE34888 Full length( BuxeA.01673.j ) 1 470
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.5373
RefSeq: YP_553001.1
UniProt: Q13QI8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLAPHYVRP ALPVPDTWAT GSSADGTLSA DATPAASRID WRSYFPDAQL QALIEQALTG NRELRIAVQR VEEARATYGI RRADTLPTIS AGGSYVRFRS PGGLLPGGSF DGEAYQVGLM ESNWELDFWG RVRNLKDAAL EEFLASDAAR RAATISVIAN VADNYLLLRE LDERIAVARE TIASRAESLR IFRRRFEVGA ISKLDLRQSE ILFQQAQTLV AQLEQSRATQ AHALAVLVGA PAPLQPAPLD DASVCPDLHA GLPSSLLERR PDIVAAEHRL RAANANIGAA RAAFFPRITL TGAVGSTSTD LQNLFASGSG AWIFTPNISL PIFDAGRNRS NLELAKAREG EAVAQYEETI QDAFRDVADA LSARVWLATQ VQAETDTLVA QSERARLARQ RYDNGATPFL EVLDAQRDLL DARQQLIQTR RALLTSRVAL YAALGGGVQD ADPAPPPSAA DITLKQGPLP
NT Sequence
ATGTCGCTCG CGCCGCACTA CGTACGCCCC GCGCTGCCGG TTCCCGACAC CTGGGCCACC GGGTCTTCCG CCGACGGAAC ATTGTCCGCG GACGCCACAC CGGCCGCCAG CCGGATCGAC TGGCGCAGCT ATTTTCCCGA CGCCCAACTG CAGGCCCTGA TCGAGCAGGC GCTGACCGGC AACCGCGAGT TGCGCATTGC CGTGCAGCGC GTCGAAGAAG CGCGTGCAAC GTACGGTATC CGGCGCGCCG ACACGCTTCC GACGATCTCC GCAGGCGGCT CCTATGTCCG TTTCCGCTCG CCGGGCGGAC TCCTGCCCGG CGGAAGTTTC GACGGTGAGG CGTATCAGGT CGGACTGATG GAAAGCAATT GGGAACTCGA CTTCTGGGGC CGCGTGCGCA ACCTCAAGGA CGCAGCCCTC GAGGAATTCC TGGCGAGCGA CGCCGCGCGC CGCGCGGCCA CCATCAGCGT GATCGCAAAC GTAGCGGACA ATTATCTGCT GCTGCGCGAA CTCGATGAAC GTATCGCCGT TGCACGCGAG ACGATCGCAA GCCGCGCCGA ATCGCTGCGA ATTTTCCGCC GCCGCTTCGA GGTCGGGGCG ATCTCGAAGC TCGATCTGCG GCAGTCGGAA ATTCTTTTTC AGCAGGCGCA AACGCTGGTT GCGCAACTGG AACAGTCACG AGCCACTCAG GCCCACGCGC TCGCCGTCCT GGTCGGCGCG CCCGCCCCGC TGCAGCCGGC GCCGCTGGAC GACGCGAGCG TATGCCCGGA TCTGCATGCG GGACTGCCTT CGTCGTTGCT GGAGAGGCGT CCGGACATTG TCGCGGCGGA ACACCGGCTG CGGGCCGCGA ACGCGAATAT CGGCGCGGCA CGCGCGGCGT TCTTTCCGCG CATCACGCTC ACGGGCGCGG TCGGCTCGAC GAGCACCGAT CTTCAGAACC TATTCGCATC GGGTAGCGGT GCGTGGATCT TCACGCCCAA CATCTCGCTG CCCATCTTCG ATGCCGGGCG CAATCGCAGC AATCTCGAAC TCGCGAAGGC CCGCGAAGGC GAAGCGGTGG CGCAATACGA AGAAACCATT CAGGACGCCT TTCGCGACGT AGCCGATGCG CTGTCGGCCA GAGTGTGGCT CGCCACGCAG GTTCAGGCGG AAACCGACAC GCTGGTGGCG CAGTCCGAAC GTGCGCGCCT TGCCAGGCAG CGATACGACA ACGGCGCGAC ACCGTTCCTG GAAGTGCTCG ACGCGCAGCG CGACCTGCTC GACGCACGGC AGCAACTGAT ACAGACGCGG CGCGCATTGC TCACGAGCCG CGTCGCGCTT TATGCCGCGC TCGGTGGAGG CGTGCAGGAC GCAGATCCGG CGCCCCCGCC TTCAGCCGCC GACATCACAC TCAAACAAGG CCCATTGCCA
Details for BuxeA.01673.j.A1.GE34888
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 68
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSLAPHYVR PALPVPDTWA TGSSADGTLS ADATPAASRI DWRSYFPDAQ LQALIEQALT GNRELRIAVQ RVEEARATYG IRRADTLPTI SAGGSYVRFR SPGGLLPGGS FDGEAYQVGL MESNWELDFW GRVRNLKDAA LEEFLASDAA RRAATISVIA NVADNYLLLR ELDERIAVAR ETIASRAESL RIFRRRFEVG AISKLDLRQS EILFQQAQTL VAQLEQSRAT QPTRSPSWSA RPPRCSRRRW TTRAYARICM RDCLRRCWRG VRTLSRRNTG CGPRTRISAR HARRSFRXHA HGRGRXDEXR SXXXIRIG
Validated NT Sequence
tttnnnnnnt ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgtcgctc gcgccgcact acgtacgccc cgcgctgccg gttcccgaca cctgggccac cgggtcttcc gccgacggaa cattgtccgc ggacgccaca ccggccgcca gccggatcga ctggcgcagc tattttcccg acgcccaact gcaggccctg atcgagcagg cgctgaccgg caaccgcgag ttgcgcattg ccgtgcagcg cgtcgaagaa gcgcgtgcaa cgtacggtat ccggcgcgcc gacacgcttc cgacgatctc cgcaggcggc tcctatgtcc gtttccgctc gccgggcgga ctcctgcccg gcggaagttt cgacggtgag gcgtatcagg tcggactgat ggaaagcaat tgggaactcg acttctgggg ccgcgtgcgc aacctcaagg acgcagccct cgaggaattc ctggcgagcg acgccgcgcg ccgcgcggcc accatcagcg tgatcgcaaa cgtagcggac aattatctgc tgctgcgcga actcgatgaa cgtatcgccg ttgcacgcga gacgatcgca agccgcgccg aatcgctgcg aattttccgc cgccgcttcg aggtcggggc gatctcgaag ctcgatctgc ggcagtcgga aattcttttt cagcaggcgc aaacgctggt tgcgcaactg gaacagtcac gagccactca gcccacgcgc tcgccgtcct ggtcggcgcg cccgccccgc tgcagccggc gccgctggac gacgcgagcg tatgcccgga tctgcatgcg ggactgcctt cgtcgttgct ggagaggcgt ccggacattg tcgcggcgga acaccggctg cgggccgcga acgcgaatat cggcgcggca cgcgcggcgt tctttccgcn atcacgctca cgggcgcggt cggntcgacg agcancgatc ntnnnnanct attcgcatcg ggtagnngng cgnngnnnnt nnnnnccnac atctnnntgn cnntcttnna tgcnggnnna nnncagcaat nnncnantnn nnannnccnn nggnnnnnng nnnnncannn nnannnannn nttnnnnnnn ccnnnnnnnn ncna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLAPHYVR PALPVPDTWA TGSSADGTLS ADATPAASRI DWRSYFPDAQ LQALIEQALT GNRELRIAVQ RVEEARATYG IRRADTLPTI SAGGSYVRFR SPGGLLPGGS FDGEAYQVGL MESNWELDFW GRVRNLKDAA LEEFLASDAA RRAATISVIA NVADNYLLLR ELDERIAVAR ETIASRAESL RIFRRRFEVG AISKLDLRQS EILFQQAQTL VAQLEQSRAT QAHALAVLVG APAPLQPAPL DDASVCPDLH AGLPSSLLER RPDIVAAEHR LRAANANIGA ARAAFFPRIT LTGAVGSTST DLQNLFASGS GAWIFTPNIS LPIFDAGRNR SNLELAKARE GEAVAQYEET IQDAFRDVAD ALSARVWLAT QVQAETDTLV AQSERARLAR QRYDNGATPF LEVLDAQRDL LDARQQLIQT RRALLTSRVA LYAALGGGVQ DADPAPPPSA ADITLKQGPL P
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgctcgcg ccgcactacg tacgccccgc gctgccggtt cccgacacct gggccaccgg gtcttccgcc gacggaacat tgtccgcgga cgccacaccg gccgccagcc ggatcgactg gcgcagctat tttcccgacg cccaactgca ggccctgatc gagcaggcgc tgaccggcaa ccgcgagttg cgcattgccg tgcagcgcgt cgaagaagcg cgtgcaacgt acggtatccg gcgcgccgac acgcttccga cgatctccgc aggcggctcc tatgtccgtt tccgctcgcc gggcggactc ctgcccggcg gaagtttcga cggtgaggcg tatcaggtcg gactgatgga aagcaattgg gaactcgact tctggggccg cgtgcgcaac ctcaaggacg cagccctcga ggaattcctg gcgagcgacg ccgcgcgccg cgcggccacc atcagcgtga tcgcaaacgt agcggacaat tatctgctgc tgcgcgaact cgatgaacgt atcgccgttg cacgcgagac gatcgcaagc cgcgccgaat cgctgcgaat tttccgccgc cgcttcgagg tcggggcgat ctcgaagctc gatctgcggc agtcggaaat tctttttcag caggcgcaaa cgctggttgc gcaactggaa cagtcacgag ccactcaggc ccacgcgctc gccgtcctgg tcggcgcgcc cgccccgctg cagccggcgc cgctggacga cgcgagcgta tgcccggatc tgcatgcggg actgccttcg tcgttgctgg agaggcgtcc ggacattgtc gcggcggaac accggctgcg ggccgcgaac gcgaatatcg gcgcggcacg cgcggcgttc tttccgcgca tcacgctcac gggcgcggtc ggctcgacga gcaccgatct tcagaaccta ttcgcatcgg gtagcggtgc gtggatcttc acgcccaaca tctcgctgcc catcttcgat gccgggcgca atcgcagcaa tctcgaactc gcgaaggccc gcgaaggcga agcggtggcg caatacgaag aaaccattca ggacgccttt cgcgacgtag ccgatgcgct gtcggccaga gtgtggctcg ccacgcaggt tcaggcggaa accgacacgc tggtggcgca gtccgaacgt gcgcgccttg ccaggcagcg atacgacaac ggcgcgacac cgttcctgga agtgctcgac gcgcagcgcg acctgctcga cgcacggcag caactgatac agacgcggcg cgcattgctc acgagccgcg tcgcgcttta tgccgcgctc ggtggaggcg tgcaggacgc agatccggcg cccccgcctt cagccgccga catcacactc aaacaaggcc cattgccata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a