BuxeA.01673.h

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuxeA.01673.h
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.h.A2.GE31998 Mature protein( BuxeA.01673.h ) 26 497
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.5726
RefSeq: YP_553324.1
UniProt: Q13PL5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTIYPTRLA GALLALSTVL MGGCMVGPDY HRPPVATPAM WKELPGWIEA QPAAEVPKGD WWTAFNDPLI NELEPLVSVS NQTVRQDYAN YQQALAEVRL ARSALFPTIG INGSATRERS STGNLSSSVL AGGAGVGNFQ RINNAGSLEA NASWAPDLWG LVRRNIEQNA ATAQASQATL ANATLSEQIA LANAVIDLRV TDANIDLLTH TVDAFEQSLR VVADQDKAGT VPPSDLVSAQ TQLESAQSSL IALGVARAQY VHAIAVLVGR NPEELDVPTN TTLPTLPSVP VSVPSTLLQR RPDIAAAERQ MAAQNAAIGV AVAAYYPTIS LSALDGFTQS PLAGLLHIAN YVWSLGGQAS ETLFDGGQRS AQVDAAKASY DAALANYRGT VLTAFQGVEN DLAGLRILAQ QAEVLERAVR DATRGTEIAR NEYQAGTVDF TTVATAQATQ LNLQQTALNV QQTRLLDAAS LIGDLGGGWS DSELGDPRKM GQRQVSP
NT Sequence
ATGAAAACGA TCTACCCCAC CAGACTGGCC GGCGCATTGC TGGCGTTATC GACCGTGTTG ATGGGCGGCT GCATGGTCGG CCCCGACTAC CATCGGCCGC CCGTCGCCAC GCCCGCCATG TGGAAGGAAC TGCCCGGCTG GATCGAGGCG CAGCCCGCCG CCGAGGTCCC GAAGGGAGAC TGGTGGACGG CCTTCAACGA CCCGCTCATC AACGAGCTCG AACCGCTCGT CTCGGTGTCG AACCAGACCG TGCGCCAGGA CTATGCGAAC TACCAGCAGG CGCTGGCCGA AGTGCGGCTC GCGCGCAGCG CGCTGTTCCC GACCATCGGC ATTAACGGAT CGGCCACCCG TGAACGTTCG TCCACGGGCA ATCTGAGCAG CAGCGTGCTG GCCGGCGGCG CGGGCGTGGG CAACTTCCAG CGCATCAACA ACGCCGGCTC GCTCGAAGCC AACGCGAGCT GGGCGCCCGA CTTGTGGGGT CTCGTGCGCC GCAACATCGA GCAAAACGCC GCCACCGCGC AGGCCAGCCA GGCCACGCTC GCCAACGCCA CGCTCTCCGA GCAGATCGCG CTGGCCAACG CGGTGATCGA TCTGCGCGTG ACGGATGCGA ATATCGACCT GCTCACTCAC ACCGTGGATG CGTTCGAGCA ATCGTTGCGC GTGGTCGCCG ATCAGGACAA GGCCGGCACC GTGCCGCCTT CCGATCTGGT GAGCGCGCAG ACGCAACTGG AGTCGGCCCA ATCGAGTCTG ATTGCACTGG GTGTCGCGCG GGCTCAATAC GTTCATGCGA TCGCCGTGCT GGTCGGCAGG AATCCGGAAG AACTGGACGT ACCCACCAAC ACCACCCTGC CCACCTTGCC GTCCGTTCCG GTCAGCGTAC CGTCCACGCT ATTGCAGCGC CGGCCCGATA TTGCCGCCGC CGAGCGCCAG ATGGCCGCAC AGAATGCCGC GATCGGCGTG GCCGTCGCGG CGTACTACCC GACGATTTCA CTATCGGCGC TGGATGGCTT CACGCAGTCG CCGTTGGCGG GCCTGCTGCA CATCGCCAAT TATGTCTGGT CGTTGGGCGG CCAGGCGAGC GAGACGCTTT TCGACGGCGG GCAGCGCAGC GCGCAGGTCG ACGCGGCAAA AGCGTCTTAC GACGCAGCCC TCGCCAATTA TCGCGGCACG GTTCTGACGG CCTTCCAGGG CGTGGAGAAC GACCTCGCCG GCTTGCGGAT CCTCGCGCAG CAAGCCGAGG TGCTGGAGCG CGCGGTGCGC GACGCGACGC GCGGCACCGA GATCGCGCGC AATGAATATC AGGCGGGCAC GGTGGACTTC ACCACCGTCG CGACCGCGCA AGCCACTCAG TTGAATCTTC AGCAGACGGC CTTGAACGTC CAGCAGACGC GGCTGCTCGA CGCCGCGTCG CTGATCGGCG ATCTGGGCGG CGGCTGGTCC GACAGCGAGC TTGGCGACCC CCGGAAGATG GGCCAACGGC AGGTGTCGCC C
Details for BuxeA.01673.h.A2.GE31998
HARVESTED ON: 4/5/2011
SEQUENCED ON: 4/11/2011
EXPECTED MW: 52kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 11
PERCENT COVERAGE: 54
Validated AA Sequence
MFTXXPXXXX XXXXXXXXXX XXXXSWFDGR PRLPSAARXH ARHXERTARX XRGSAXRXXX EGRLXXGLQQ PXSTXSXRSS RCXTTXXXXX MXTXXXXWPX XXXXAXRXXR XXXXXXXXXX XXXSEXXRXG XWAXSXXXXS XXXXXXXXGA RLXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXSXXXIXX XXXRSXXGRH RXXXXSGERA XATGVGPIEX XCTGCRAXSI XSXXXVLVGG XXXXXXXPPT
Validated NT Sequence
cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccatgtt tacnnnnnct cctnnnnnnn annnnnannn aannanaann ngnnnnnann nnnaantcaa ncannggtcc tggttcgatg gncggccccg actaccatcg gccgcccgtc nccacgcccg ccatgnggaa cgaactgcnc ggntggntcg aggctcagcc cnncgccnan gnnccgaagg gagactggng nacggncttc aacaacccnn ntcaacnnnn tcnanncgnt cgtctcggtg tcnaaccaca ncnngnncnn ngantatgcn aactannnnn nngnnctggc cgaanngnnn ntnnnnngcn nnccgctnnn nncgannnnn nnnattnnnn nntcnnncnn tcnnnnnnng gnnatctgag cnnnnncgng ntggcnngtg ggcannttcn annnnntnna natcncnngn tcnnnnnnnn ncnannnngg gcgccagact tgnnnnnnnn cntgnnnnnn nncntcnann angncnnnnn ngnnnangnn anncnngnnn nnnnnntcnn nnncanncan canatnnnnn tnnnncannc nnngntcncn cnnnncnngn ngncnaannc cnanntgnnc nnnnnnnncn ngnntcnnnn annncaatcn ntnnnnnnnn nnnncgatca ngacnaggcc ggcaccgngn nnccnnnnna tctggtgagc gcgcnnacgc aactggagtc ggnccnatcg agtnngantg cactgggtgt cgcgcgngct caatacnttc atgnnnnnnn gtgctggtcg gaggaancnn naanaantnn nnnnanccnc caacnnca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGPDYHRP PVATPAMWKE LPGWIEAQPA AEVPKGDWWT AFNDPLINEL EPLVSVSNQT VRQDYANYQQ ALAEVRLARS ALFPTIGING SATRERSSTG NLSSSVLAGG AGVGNFQRIN NAGSLEANAS WAPDLWGLVR RNIEQNAATA QASQATLANA TLSEQIALAN AVIDLRVTDA NIDLLTHTVD AFEQSLRVVA DQDKAGTVPP SDLVSAQTQL ESAQSSLIAL GVARAQYVHA IAVLVGRNPE ELDVPTNTTL PTLPSVPVSV PSTLLQRRPD IAAAERQMAA QNAAIGVAVA AYYPTISLSA LDGFTQSPLA GLLHIANYVW SLGGQASETL FDGGQRSAQV DAAKASYDAA LANYRGTVLT AFQGVENDLA GLRILAQQAE VLERAVRDAT RGTEIARNEY QAGTVDFTTV ATAQATQLNL QQTALNVQQT RLLDAASLIG DLGGGWSDSE LGDPRKMGQR QVSP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtcggcccc gactaccatc ggccgcccgt cgccacgccc gccatgtgga aggaactgcc cggctggatc gaggcgcagc ccgccgccga ggtcccgaag ggagactggt ggacggcctt caacgacccg ctcatcaacg agctcgaacc gctcgtctcg gtgtcgaacc agaccgtgcg ccaggactat gcgaactacc agcaggcgct ggccgaagtg cggctcgcgc gcagcgcgct gttcccgacc atcggcatta acggatcggc cacccgtgaa cgttcgtcca cgggcaatct gagcagcagc gtgctggccg gcggcgcggg cgtgggcaac ttccagcgca tcaacaacgc cggctcgctc gaagccaacg cgagctgggc gcccgacttg tggggtctcg tgcgccgcaa catcgagcaa aacgccgcca ccgcgcaggc cagccaggcc acgctcgcca acgccacgct ctccgagcag atcgcgctgg ccaacgcggt gatcgatctg cgcgtgacgg atgcgaatat cgacctgctc actcacaccg tggatgcgtt cgagcaatcg ttgcgcgtgg tcgccgatca ggacaaggcc ggcaccgtgc cgccttccga tctggtgagc gcgcagacgc aactggagtc ggcccaatcg agtctgattg cactgggtgt cgcgcgggct caatacgttc atgcgatcgc cgtgctggtc ggcaggaatc cggaagaact ggacgtaccc accaacacca ccctgcccac cttgccgtcc gttccggtca gcgtaccgtc cacgctattg cagcgccggc ccgatattgc cgccgccgag cgccagatgg ccgcacagaa tgccgcgatc ggcgtggccg tcgcggcgta ctacccgacg atttcactat cggcgctgga tggcttcacg cagtcgccgt tggcgggcct gctgcacatc gccaattatg tctggtcgtt gggcggccag gcgagcgaga cgcttttcga cggcgggcag cgcagcgcgc aggtcgacgc ggcaaaagcg tcttacgacg cagccctcgc caattatcgc ggcacggttc tgacggcctt ccagggcgtg gagaacgacc tcgccggctt gcggatcctc gcgcagcaag ccgaggtgct ggagcgcgcg gtgcgcgacg cgacgcgcgg caccgagatc gcgcgcaatg aatatcaggc gggcacggtg gacttcacca ccgtcgcgac cgcgcaagcc actcagttga atcttcagca gacggccttg aacgtccagc agacgcggct gctcgacgcc gcgtcgctga tcggcgatct gggcggcggc tggtccgaca gcgagcttgg cgacccccgg aagatgggcc aacggcaggt gtcgccctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa