BuxeA.01673.g

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuxeA.01673.g
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.g.A2.GE34886 Mature protein( BuxeA.01673.g ) 29 485
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.6363
RefSeq: YP_553923.1
UniProt: Q13MW6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFSLSLSRPL PRRALAATLI GALLSACSTL PPYQRPSAGI PAHYAGAAPG WAQAAPGDAA PRGPWWTIFN DPVLDQLEAR IDVSNQTVRK AVAQVQQARS MVDYQRAGFF PTITAGVAQD RYRTSQNVAG KSLAGKTVPD YSAGVAASWE PDLFGRVRDS VNGAQADAQA SEADLEGVRL AMSADLAIDY FSLRSLDTQK KLLDDSVSAY AAALKLLQQQ LKNGAIDASA VAQATTQLEA TRTQDTDIDV TRSQMQHAIA TLIGEPASSF TLPPNGSAVT PPAIPPGLPS QLLERRPDIA AAERRVASAN AQIGVAHAAF FPDLVLSASA GLESSFFAPW LSAPSLFWSL GPQLAGTLFD GGKRKASLHG ATAQYDGAVA DYRQSVLVAF QQVEDNLSAL HSLADEAVSQ QRATTAADLS LRLTTNRFNA GAVSYLDVVT AQTIALSNQR IADQIAARQL ESSVLLLEAL GGTWGNGSSG AAGAA
NT Sequence
ATGTTCTCCC TGTCTCTGTC ACGACCGCTG CCGCGCCGCG CGCTGGCGGC CACGCTGATC GGCGCGCTGC TGAGCGCGTG CTCGACCCTG CCGCCCTATC AGCGCCCGAG CGCCGGGATT CCCGCGCACT ATGCGGGCGC CGCGCCCGGC TGGGCGCAAG CCGCGCCAGG CGACGCCGCA CCGCGCGGCC CCTGGTGGAC GATCTTCAAC GATCCGGTGC TCGACCAGCT CGAAGCGCGC ATCGACGTAT CGAACCAGAC CGTGCGCAAA GCGGTCGCGC AGGTTCAGCA GGCGCGCTCG ATGGTGGACT ATCAGCGCGC CGGCTTCTTC CCGACGATCA CCGCCGGTGT CGCGCAGGAT CGCTACCGGA CCTCGCAGAA CGTCGCAGGA AAGTCGCTCG CGGGCAAGAC CGTGCCGGAC TATTCGGCGG GCGTGGCGGC GAGCTGGGAG CCGGATCTGT TCGGTCGGGT GCGCGACAGT GTGAACGGTG CGCAGGCCGA CGCGCAGGCG AGCGAAGCGG ACCTCGAAGG CGTGCGCCTC GCGATGAGCG CGGATCTGGC CATCGACTAT TTCTCGCTGC GCTCGCTCGA CACGCAAAAG AAGCTGCTCG ACGACAGCGT GAGCGCCTAC GCCGCCGCGT TGAAGCTCCT CCAGCAGCAG CTGAAAAACG GCGCGATCGA CGCCTCCGCC GTCGCGCAGG CCACGACGCA ACTCGAAGCG ACGCGCACCC AGGACACCGA CATCGACGTG ACACGCTCGC AGATGCAGCA CGCCATCGCC ACGCTGATCG GCGAGCCTGC TTCGAGCTTC ACGCTGCCGC CGAACGGCTC GGCCGTGACG CCGCCCGCGA TTCCGCCCGG CTTGCCGTCG CAACTGCTGG AACGGCGCCC CGATATCGCC GCCGCCGAAC GGCGCGTGGC GTCGGCGAAC GCACAGATCG GCGTCGCGCA TGCGGCGTTT TTCCCGGACC TGGTGCTGTC CGCGAGCGCG GGGCTCGAAA GCTCGTTTTT CGCGCCGTGG CTGAGCGCGC CGAGCCTGTT CTGGTCGCTC GGTCCGCAAC TGGCCGGCAC GCTGTTCGAC GGCGGCAAAC GCAAGGCCAG CCTGCACGGC GCCACCGCGC AATACGACGG CGCCGTGGCG GATTATCGTC AGTCGGTGCT GGTGGCGTTC CAGCAGGTCG AAGACAACCT CTCCGCGCTG CATTCCCTCG CCGACGAAGC CGTCAGCCAG CAGCGCGCCA CCACCGCCGC GGATCTGTCG TTGCGGCTCA CCACGAACCG TTTCAACGCG GGCGCGGTCA GCTATCTCGA CGTGGTCACG GCGCAGACGA TCGCGCTCTC GAACCAGCGG ATCGCGGATC AGATCGCCGC GCGGCAGCTG GAGTCGAGTG TGCTGTTGCT GGAGGCGTTG GGCGGGACGT GGGGCAATGG CAGCAGCGGC GCGGCGGGTG CGGCT
Details for BuxeA.01673.g.A2.GE34886
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/19/2012
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 81
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTLPPYQRP SAGIPAHYAG AAPGWAQAAP GDAAPRGPWW TIFNDPVLDQ LEARIDVSNQ TVRKAVAQVQ QARSMVDYQR AGFFPTITAG VAQDRYRTSQ NVAGKSLAGK TVPDYSAGVA ASWEPDLFGR VRDSVNGAQA DAQASEADLE GVRLAMSADL AIDYFSLRSL DTQKKLLDDS VSAYAAALKL LQQQLKNGAI DASAVAQATT QLEATRTQDT DIDVTRSQMQ HAIATLIGEP ASSFTLPPNG SAVTPPAIPP GLPSQLLERR PDIAAAERRV AXXTHRSASR MXVFPXLXXS XSXGXEXXFX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
ttntnnnntt taagaaggag anataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgaccctgc cgccctatca gcgcccgagc gccgggattc ccgcgcacta tgcgggcgcc gcgcccggct gggcgcaagc cgcgccaggc gacgccgcac cgcgcggccc ctggtggacg atcttcaacg atccggtgct cgaccagctc gaagcgcgca tcgacgtatc gaaccagacc gtgcgcaaag cggtcgcgca ggttcagcag gcgcgctcga tggtggacta tcagcgcgcc ggcttcttcc cgacgatcac cgccggtgtc gcgcaggatc gctaccggac ctcgcagaac gtcgcaggaa agtcgctcgc gggcaagacc gtgccggact attcggcggg cgtggcggcg agctgggagc cggatctgtt cggtcgggtg cgcgacagtg tgaacggtgc gcaggccgac gcgcaggcga gcgaagcgga cctcgaaggc gtgcgcctcg cgatgagcgc ggatctggcc atcgactatt tctcgctgcg ctcgctcgac acgcaaaaga agctgctcga cgacagcgtg agcgcctacg ccgccgcgtt gaagctcctc cagcagcagc tgaaaaacgg cgcgatcgac gcctccgccg tcgcgcaggc cacgacgcaa ctcgaagcga cgcgcaccca ggacaccgac atcgacgtga cacgctcgca gatgcagcac gccatcgcca cgctgatcgg cgagcctgct tcgagcttca cgctgccgcc gaacggctcg gccgtgacgc cgcccgcgat tccgcccggn ttgccgtcgc aactgctgga acggcgcccc gatatcgccg ccgccgaacg gcgcgtggcg tnnnnaacgc acagatcggc ntcgcgcatg nnngttttcc cnganctggn gntgtccncg agcgnngggn tcgaaagnnc gtttttnnnn ncnnnnngan nnncnanccn gntcnnnnnn ntcnnnncnn annngnnngn nnnctnttnn nnnngnnnnn nnnnnnnngn nnggncnncc nnnnannnnn nnnnnnnnnn nnnnnnnnnn nngnnnnnnn nnnnnnnnan nnnnnnnnnn nnnnaannnn nnannnnnnn ngnnnnnnnc g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTLPPYQRP SAGIPAHYAG AAPGWAQAAP GDAAPRGPWW TIFNDPVLDQ LEARIDVSNQ TVRKAVAQVQ QARSMVDYQR AGFFPTITAG VAQDRYRTSQ NVAGKSLAGK TVPDYSAGVA ASWEPDLFGR VRDSVNGAQA DAQASEADLE GVRLAMSADL AIDYFSLRSL DTQKKLLDDS VSAYAAALKL LQQQLKNGAI DASAVAQATT QLEATRTQDT DIDVTRSQMQ HAIATLIGEP ASSFTLPPNG SAVTPPAIPP GLPSQLLERR PDIAAAERRV ASANAQIGVA HAAFFPDLVL SASAGLESSF FAPWLSAPSL FWSLGPQLAG TLFDGGKRKA SLHGATAQYD GAVADYRQSV LVAFQQVEDN LSALHSLADE AVSQQRATTA ADLSLRLTTN RFNAGAVSYL DVVTAQTIAL SNQRIADQIA ARQLESSVLL LEALGGTWGN GSSGAAGAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccctgccg ccctatcagc gcccgagcgc cgggattccc gcgcactatg cgggcgccgc gcccggctgg gcgcaagccg cgccaggcga cgccgcaccg cgcggcccct ggtggacgat cttcaacgat ccggtgctcg accagctcga agcgcgcatc gacgtatcga accagaccgt gcgcaaagcg gtcgcgcagg ttcagcaggc gcgctcgatg gtggactatc agcgcgccgg cttcttcccg acgatcaccg ccggtgtcgc gcaggatcgc taccggacct cgcagaacgt cgcaggaaag tcgctcgcgg gcaagaccgt gccggactat tcggcgggcg tggcggcgag ctgggagccg gatctgttcg gtcgggtgcg cgacagtgtg aacggtgcgc aggccgacgc gcaggcgagc gaagcggacc tcgaaggcgt gcgcctcgcg atgagcgcgg atctggccat cgactatttc tcgctgcgct cgctcgacac gcaaaagaag ctgctcgacg acagcgtgag cgcctacgcc gccgcgttga agctcctcca gcagcagctg aaaaacggcg cgatcgacgc ctccgccgtc gcgcaggcca cgacgcaact cgaagcgacg cgcacccagg acaccgacat cgacgtgaca cgctcgcaga tgcagcacgc catcgccacg ctgatcggcg agcctgcttc gagcttcacg ctgccgccga acggctcggc cgtgacgccg cccgcgattc cgcccggctt gccgtcgcaa ctgctggaac ggcgccccga tatcgccgcc gccgaacggc gcgtggcgtc ggcgaacgca cagatcggcg tcgcgcatgc ggcgtttttc ccggacctgg tgctgtccgc gagcgcgggg ctcgaaagct cgtttttcgc gccgtggctg agcgcgccga gcctgttctg gtcgctcggt ccgcaactgg ccggcacgct gttcgacggc ggcaaacgca aggccagcct gcacggcgcc accgcgcaat acgacggcgc cgtggcggat tatcgtcagt cggtgctggt ggcgttccag caggtcgaag acaacctctc cgcgctgcat tccctcgccg acgaagccgt cagccagcag cgcgccacca ccgccgcgga tctgtcgttg cggctcacca cgaaccgttt caacgcgggc gcggtcagct atctcgacgt ggtcacggcg cagacgatcg cgctctcgaa ccagcggatc gcggatcaga tcgccgcgcg gcagctggag tcgagtgtgc tgttgctgga ggcgttgggc gggacgtggg gcaatggcag cagcggcgcg gcgggtgcgg cttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa