BuxeA.01673.d

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuxeA.01673.d
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.d.A2.GE31994 Mature protein( BuxeA.01673.d ) 27 537
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.d.A2.PS01251 Mature protein( BuxeA.01673.d ) 27 537

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.6930
RefSeq: YP_554453.1
UniProt: Q13LD6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRFESLSGW GRAAASGLLV ALLAACSVEP TYKRPEVDTP VAFKEAPAAS APSAASAPSA GSAQDNGTWK QAQPADDAHR GEWWTIFGDP QLNALEEQAA AANQDLKAAA ARVQQARAVT QAARSDWFPK LDAGFGPTRE RASAASQFLP DNAGGTNGTI WRAQVGASYE ADLFGRVGSN VNASRADEQQ SEALFRSVQL SLQADVAQNY FQLRELDTDQ DLYRRTVALR EDTLKLVERR FKEGDIGELD VSRARNELAS ARADAVGVAR QRAASEHSLA ILLGKPPADF SFAEAPLVPV TVRVPPGLPS ALLERRPDIS AAERAMQAAN ARVGLAKSAF FPKLDITGAA GFESATLGDL FMWSSRAFIL GPFAGTALTL PLFDGGRRKA NLAQARSKYD EDVAQYRQQV LVAFREVEDN LADLRLLDDQ MREQNDAVQA SQRAAHLSRT QYTEGAVSYL DVIDGERQVL ISQLQASHLS GTQAVATVNL IRALGGGWGG VNAPDTAVGA VAPASAPVAA VAASAPASAS VQQVAKQ
NT Sequence
ATGAAACGCT TTGAATCTTT GAGTGGGTGG GGCCGGGCGG CTGCCAGCGG TTTGCTGGTT GCATTGCTCG CTGCCTGCTC CGTCGAGCCG ACTTATAAAC GGCCTGAAGT GGATACGCCG GTTGCGTTCA AGGAAGCGCC CGCTGCGTCA GCGCCGTCTG CGGCGTCCGC GCCGTCGGCT GGATCGGCGC AGGACAACGG CACCTGGAAG CAGGCGCAGC CCGCCGACGA CGCGCATCGC GGCGAATGGT GGACGATTTT CGGCGACCCG CAGCTCAACG CGCTCGAAGA GCAGGCGGCC GCCGCCAATC AGGACCTGAA GGCCGCCGCG GCGCGCGTGC AGCAGGCGCG GGCGGTGACG CAGGCGGCCA GGTCGGACTG GTTCCCGAAG CTCGACGCGG GCTTTGGCCC AACGCGCGAG CGTGCTTCGG CGGCGTCGCA ATTCCTGCCG GACAACGCGG GCGGCACGAA CGGCACGATC TGGCGCGCGC AGGTTGGCGC ATCGTACGAG GCGGATCTGT TCGGGCGGGT CGGTTCGAAC GTGAACGCGT CGCGGGCGGA CGAGCAGCAA AGCGAAGCGC TGTTCCGTTC CGTGCAACTG TCGTTGCAGG CGGACGTCGC GCAGAACTAC TTCCAGTTGC GCGAGCTCGA TACGGACCAG GACCTGTACC GCCGCACCGT GGCGTTGCGC GAGGATACGC TGAAGCTCGT CGAACGGCGT TTCAAGGAAG GCGATATCGG CGAACTGGAT GTCTCGCGGG CCCGCAACGA ACTCGCGAGT GCGCGTGCGG ACGCCGTGGG CGTGGCGCGT CAGCGTGCCG CGTCGGAGCA TAGCCTCGCG ATTCTGCTCG GCAAGCCGCC GGCGGATTTC TCGTTCGCGG AAGCGCCGCT GGTGCCGGTG ACGGTGCGGG TGCCGCCGGG TCTGCCCTCG GCGTTGCTTG AGCGCCGCCC GGATATTTCG GCTGCGGAAC GGGCGATGCA GGCCGCGAAC GCGCGTGTCG GCCTGGCGAA ATCGGCGTTC TTCCCGAAGC TCGACATCAC CGGTGCAGCT GGTTTTGAGT CGGCGACGCT CGGCGATCTG TTCATGTGGT CGAGCCGGGC GTTCATTCTC GGGCCGTTTG CCGGCACGGC GTTGACGCTG CCTTTGTTCG ACGGCGGCCG CCGCAAGGCG AATCTCGCGC AAGCCCGTTC GAAGTACGAC GAGGACGTTG CGCAGTATCG TCAACAGGTG CTGGTGGCGT TCCGTGAAGT CGAAGACAAC CTCGCCGATT TGCGCCTGCT CGACGATCAG ATGCGCGAGC AGAACGATGC GGTGCAGGCA TCGCAACGGG CGGCGCATCT GTCGCGTACG CAGTACACGG AAGGCGCGGT CAGCTATCTG GACGTGATCG ATGGCGAGCG GCAGGTGCTG ATTTCGCAGT TGCAGGCGAG CCATCTGTCG GGCACGCAGG CGGTGGCGAC GGTTAATCTG ATTCGCGCGC TGGGCGGTGG CTGGGGCGGC GTGAATGCGC CGGATACGGC GGTGGGCGCG GTTGCGCCGG CATCGGCTCC TGTGGCGGCG GTTGCTGCGT CGGCGCCCGC TTCCGCTTCC GTGCAGCAGG TGGCGAAGCA G
Details for BuxeA.01673.d.A2.GE31994
HARVESTED ON: 4/5/2011
SEQUENCED ON: 4/11/2011
EXPECTED MW: 56kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 67
Validated AA Sequence
XXXXXXXXXX GPGSMSVEPT YKRPEVDTPV AFKEAPAASA XXXASAPSAG SAQDNGTWKQ XXXADDAHRG EWWTIFGDPQ LNALEEQAAA ANQDLKAAAA RVQQARAVTQ AARSDWFPKL DAGFGPTRER ASAASQFLPD NAGGTNGTIW RAQVGASYEA DLFGRVGSNV XXSRADEQQS EXLFRSVQLS LQADVAXNYF QLRELDTDQD LYRRTVALRE DXXKLVXRRF KEXDIGELDV SRARNXTREC XCGRXGRXAX XXRXXX
Validated NT Sequence
tcgccacctg ctgcacggaa gcggaagcgg gcgccgacgc agcaaccgcc gccacaggag ccgatgccgg cgcaaccgcg cccaccgccg tatccggcgc attcacgccg ccccagccac cgcccagcgc gcgaatcaga ttaaccgtcg ccaccgcctg cgtgcccgac agatggctcg cctgcaactg cgaaatcagc acctgccgct cgccatcgat cacgtccaga tagctgaccg cgccttccgt gtactgcgta cgcgacagat gcgccgcccg ttgcgatgcc tgcaccgcat cgttctgctc gcgcatctga tcgtcgagca ggcgcaaatc ggcgaggttg tcttcgactt cacggaacgc caccagcacc tgttgacgat actgcgcaac gtcctcgtcg tacttcgaac gggcttgcgc gagattcgcc ttgcggcggc cgccgtcgaa caaaggcagc gtcaacgccg tgccggcaaa cggcccgaga atgaacgccc ggctcgacca catgaacaga tcgccgagcg tcgccgactc aaaaccagct gcaccggtga tgtcgagctt cgggaagaac gccgatttcg ccaggccgac acgcgcgttc gcggcctgca tcgcccgttc cgcagccgaa atatccgggc ggcgctcaag caacgccgag ggcagacccg gcggcacccg caccgtcacc ggcaccagcg gcgcttccgc gaacgagaaa tccgccggcg gcttgccgag cagaatcgcg aggctatgct ccgacgcggc acgctgacgc gccacgccca cggcgtccgc acgcgcactc gcgagttcgt tgcgggcccg cgagacatcc agttcgccga tatcgcnntc cttgaaacgc cgttcgacga gcttcagcgt atcctcgcgc aacgncacgg tgcggcggta caggtccngg tccgtatcga gctcgcgcaa ctggaagtag ttntgcgcga cgtccgcctg cancnacagt tgcacggaac ggaacagcgn ttcgctttgc tgctcgtccg cccgcgnngn gntcacgttc gaaccgaccc gcccgaacag atccgcctcg tacgatgcgc caacctgcgc gcgncagatc gtgccgttcg tnccgcccgc gttgtccggc aggaattgcg acgccgccga agcacgctcg cgcgttgggc caaagcccgc gtcgagcttc gggaaccagt ccgacctggc cgcctgcgtc accgcccgcg cctgctgcac gcgcgccgcg gcggccttca ggtcctgatt ggcggcggcc gcctgctctt cgagcgcgtt gagctgcggg tcgccgaaaa tcgtccacca ttcgccgcga tgcgcgtcgt cggc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVEPTYKR PEVDTPVAFK EAPAASAPSA ASAPSAGSAQ DNGTWKQAQP ADDAHRGEWW TIFGDPQLNA LEEQAAAANQ DLKAAAARVQ QARAVTQAAR SDWFPKLDAG FGPTRERASA ASQFLPDNAG GTNGTIWRAQ VGASYEADLF GRVGSNVNAS RADEQQSEAL FRSVQLSLQA DVAQNYFQLR ELDTDQDLYR RTVALREDTL KLVERRFKEG DIGELDVSRA RNELASARAD AVGVARQRAA SEHSLAILLG KPPADFSFAE APLVPVTVRV PPGLPSALLE RRPDISAAER AMQAANARVG LAKSAFFPKL DITGAAGFES ATLGDLFMWS SRAFILGPFA GTALTLPLFD GGRRKANLAQ ARSKYDEDVA QYRQQVLVAF REVEDNLADL RLLDDQMREQ NDAVQASQRA AHLSRTQYTE GAVSYLDVID GERQVLISQL QASHLSGTQA VATVNLIRAL GGGWGGVNAP DTAVGAVAPA SAPVAAVAAS APASASVQQV AKQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgtcgag ccgacttata aacggcctga agtggatacg ccggttgcgt tcaaggaagc gcccgctgcg tcagcgccgt ctgcggcgtc cgcgccgtcg gctggatcgg cgcaggacaa cggcacctgg aagcaggcgc agcccgccga cgacgcgcat cgcggcgaat ggtggacgat tttcggcgac ccgcagctca acgcgctcga agagcaggcg gccgccgcca atcaggacct gaaggccgcc gcggcgcgcg tgcagcaggc gcgggcggtg acgcaggcgg ccaggtcgga ctggttcccg aagctcgacg cgggctttgg cccaacgcgc gagcgtgctt cggcggcgtc gcaattcctg ccggacaacg cgggcggcac gaacggcacg atctggcgcg cgcaggttgg cgcatcgtac gaggcggatc tgttcgggcg ggtcggttcg aacgtgaacg cgtcgcgggc ggacgagcag caaagcgaag cgctgttccg ttccgtgcaa ctgtcgttgc aggcggacgt cgcgcagaac tacttccagt tgcgcgagct cgatacggac caggacctgt accgccgcac cgtggcgttg cgcgaggata cgctgaagct cgtcgaacgg cgtttcaagg aaggcgatat cggcgaactg gatgtctcgc gggcccgcaa cgaactcgcg agtgcgcgtg cggacgccgt gggcgtggcg cgtcagcgtg ccgcgtcgga gcatagcctc gcgattctgc tcggcaagcc gccggcggat ttctcgttcg cggaagcgcc gctggtgccg gtgacggtgc gggtgccgcc gggtctgccc tcggcgttgc ttgagcgccg cccggatatt tcggctgcgg aacgggcgat gcaggccgcg aacgcgcgtg tcggcctggc gaaatcggcg ttcttcccga agctcgacat caccggtgca gctggttttg agtcggcgac gctcggcgat ctgttcatgt ggtcgagccg ggcgttcatt ctcgggccgt ttgccggcac ggcgttgacg ctgcctttgt tcgacggcgg ccgccgcaag gcgaatctcg cgcaagcccg ttcgaagtac gacgaggacg ttgcgcagta tcgtcaacag gtgctggtgg cgttccgtga agtcgaagac aacctcgccg atttgcgcct gctcgacgat cagatgcgcg agcagaacga tgcggtgcag gcatcgcaac gggcggcgca tctgtcgcgt acgcagtaca cggaaggcgc ggtcagctat ctggacgtga tcgatggcga gcggcaggtg ctgatttcgc agttgcaggc gagccatctg tcgggcacgc aggcggtggc gacggttaat ctgattcgcg cgctgggcgg tggctggggc ggcgtgaatg cgccggatac ggcggtgggc gcggttgcgc cggcatcggc tcctgtggcg gcggttgctg cgtcggcgcc cgcttccgct tccgtgcagc aggtggcgaa gcagtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BuxeA.01673.d.A2.PS01251
PURIFICATION DATe: 10/26/2011
CONCENTRATION: 23.3mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 100µl
PERCENT IDENTITY: 94
PERCENT COVERAGE: 67
Protocol Notes
notes unavailable
Validated AA Sequence
XXXXXXXXXX GPGSMSVEPT YKRPEVDTPV AFKEAPAASA XXXASAPSAG SAQDNGTWKQ XXXADDAHRG EWWTIFGDPQ LNALEEQAAA ANQDLKAAAA RVQQARAVTQ AARSDWFPKL DAGFGPTRER ASAASQFLPD NAGGTNGTIW RAQVGASYEA DLFGRVGSNV XXSRADEQQS EXLFRSVQLS LQADVAXNYF QLRELDTDQD LYRRTVALRE DXXKLVXRRF KEXDIGELDV SRARNXTREC XCGRXGRXAX XXRXXX
Validated NT Sequence
tcgccacctg ctgcacggaa gcggaagcgg gcgccgacgc agcaaccgcc gccacaggag ccgatgccgg cgcaaccgcg cccaccgccg tatccggcgc attcacgccg ccccagccac cgcccagcgc gcgaatcaga ttaaccgtcg ccaccgcctg cgtgcccgac agatggctcg cctgcaactg cgaaatcagc acctgccgct cgccatcgat cacgtccaga tagctgaccg cgccttccgt gtactgcgta cgcgacagat gcgccgcccg ttgcgatgcc tgcaccgcat cgttctgctc gcgcatctga tcgtcgagca ggcgcaaatc ggcgaggttg tcttcgactt cacggaacgc caccagcacc tgttgacgat actgcgcaac gtcctcgtcg tacttcgaac gggcttgcgc gagattcgcc ttgcggcggc cgccgtcgaa caaaggcagc gtcaacgccg tgccggcaaa cggcccgaga atgaacgccc ggctcgacca catgaacaga tcgccgagcg tcgccgactc aaaaccagct gcaccggtga tgtcgagctt cgggaagaac gccgatttcg ccaggccgac acgcgcgttc gcggcctgca tcgcccgttc cgcagccgaa atatccgggc ggcgctcaag caacgccgag ggcagacccg gcggcacccg caccgtcacc ggcaccagcg gcgcttccgc gaacgagaaa tccgccggcg gcttgccgag cagaatcgcg aggctatgct ccgacgcggc acgctgacgc gccacgccca cggcgtccgc acgcgcactc gcgagttcgt tgcgggcccg cgagacatcc agttcgccga tatcgcnntc cttgaaacgc cgttcgacga gcttcagcgt atcctcgcgc aacgncacgg tgcggcggta caggtccngg tccgtatcga gctcgcgcaa ctggaagtag ttntgcgcga cgtccgcctg cancnacagt tgcacggaac ggaacagcgn ttcgctttgc tgctcgtccg cccgcgnngn gntcacgttc gaaccgaccc gcccgaacag atccgcctcg tacgatgcgc caacctgcgc gcgncagatc gtgccgttcg tnccgcccgc gttgtccggc aggaattgcg acgccgccga agcacgctcg cgcgttgggc caaagcccgc gtcgagcttc gggaaccagt ccgacctggc cgcctgcgtc accgcccgcg cctgctgcac gcgcgccgcg gcggccttca ggtcctgatt ggcggcggcc gcctgctctt cgagcgcgtt gagctgcggg tcgccgaaaa tcgtccacca ttcgccgcga tgcgcgtcgt cggc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVEPTYKR PEVDTPVAFK EAPAASAPSA ASAPSAGSAQ DNGTWKQAQP ADDAHRGEWW TIFGDPQLNA LEEQAAAANQ DLKAAAARVQ QARAVTQAAR SDWFPKLDAG FGPTRERASA ASQFLPDNAG GTNGTIWRAQ VGASYEADLF GRVGSNVNAS RADEQQSEAL FRSVQLSLQA DVAQNYFQLR ELDTDQDLYR RTVALREDTL KLVERRFKEG DIGELDVSRA RNELASARAD AVGVARQRAA SEHSLAILLG KPPADFSFAE APLVPVTVRV PPGLPSALLE RRPDISAAER AMQAANARVG LAKSAFFPKL DITGAAGFES ATLGDLFMWS SRAFILGPFA GTALTLPLFD GGRRKANLAQ ARSKYDEDVA QYRQQVLVAF REVEDNLADL RLLDDQMREQ NDAVQASQRA AHLSRTQYTE GAVSYLDVID GERQVLISQL QASHLSGTQA VATVNLIRAL GGGWGGVNAP DTAVGAVAPA SAPVAAVAAS APASASVQQV AKQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCGTCGAG CCGACTTATA AACGGCCTGA AGTGGATACG CCGGTTGCGT TCAAGGAAGC GCCCGCTGCG TCAGCGCCGT CTGCGGCGTC CGCGCCGTCG GCTGGATCGG CGCAGGACAA CGGCACCTGG AAGCAGGCGC AGCCCGCCGA CGACGCGCAT CGCGGCGAAT GGTGGACGAT TTTCGGCGAC CCGCAGCTCA ACGCGCTCGA AGAGCAGGCG GCCGCCGCCA ATCAGGACCT GAAGGCCGCC GCGGCGCGCG TGCAGCAGGC GCGGGCGGTG ACGCAGGCGG CCAGGTCGGA CTGGTTCCCG AAGCTCGACG CGGGCTTTGG CCCAACGCGC GAGCGTGCTT CGGCGGCGTC GCAATTCCTG CCGGACAACG CGGGCGGCAC GAACGGCACG ATCTGGCGCG CGCAGGTTGG CGCATCGTAC GAGGCGGATC TGTTCGGGCG GGTCGGTTCG AACGTGAACG CGTCGCGGGC GGACGAGCAG CAAAGCGAAG CGCTGTTCCG TTCCGTGCAA CTGTCGTTGC AGGCGGACGT CGCGCAGAAC TACTTCCAGT TGCGCGAGCT CGATACGGAC CAGGACCTGT ACCGCCGCAC CGTGGCGTTG CGCGAGGATA CGCTGAAGCT CGTCGAACGG CGTTTCAAGG AAGGCGATAT CGGCGAACTG GATGTCTCGC GGGCCCGCAA CGAACTCGCG AGTGCGCGTG CGGACGCCGT GGGCGTGGCG CGTCAGCGTG CCGCGTCGGA GCATAGCCTC GCGATTCTGC TCGGCAAGCC GCCGGCGGAT TTCTCGTTCG CGGAAGCGCC GCTGGTGCCG GTGACGGTGC GGGTGCCGCC GGGTCTGCCC TCGGCGTTGC TTGAGCGCCG CCCGGATATT TCGGCTGCGG AACGGGCGAT GCAGGCCGCG AACGCGCGTG TCGGCCTGGC GAAATCGGCG TTCTTCCCGA AGCTCGACAT CACCGGTGCA GCTGGTTTTG AGTCGGCGAC GCTCGGCGAT CTGTTCATGT GGTCGAGCCG GGCGTTCATT CTCGGGCCGT TTGCCGGCAC GGCGTTGACG CTGCCTTTGT TCGACGGCGG CCGCCGCAAG GCGAATCTCG CGCAAGCCCG TTCGAAGTAC GACGAGGACG TTGCGCAGTA TCGTCAACAG GTGCTGGTGG CGTTCCGTGA AGTCGAAGAC AACCTCGCCG ATTTGCGCCT GCTCGACGAT CAGATGCGCG AGCAGAACGA TGCGGTGCAG GCATCGCAAC GGGCGGCGCA TCTGTCGCGT ACGCAGTACA CGGAAGGCGC GGTCAGCTAT CTGGACGTGA TCGATGGCGA GCGGCAGGTG CTGATTTCGC AGTTGCAGGC GAGCCATCTG TCGGGCACGC AGGCGGTGGC GACGGTTAAT CTGATTCGCG CGCTGGGCGG TGGCTGGGGC GGCGTGAATG CGCCGGATAC GGCGGTGGGC GCGGTTGCGC CGGCATCGGC TCCTGTGGCG GCGGTTGCTG CGTCGGCGCC CGCTTCCGCT TCCGTGCAGC AGGTGGCGAA GCAGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA