BuxeA.01673.c

MFS multidrug efflux pump, outer membrane lipoprotein, NodT family

CENTER ID: BuxeA.01673.c
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.c.A2.GE31993 Mature protein( BuxeA.01673.c ) 20 477
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.c.A2.PW31909 Mature protein( BuxeA.01673.c ) 20 477

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.7046
RefSeq: YP_554566.1
UniProt: Q13L23

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKPILLKVLA SAALLTLAAC AVQPATHADL PQTVKTVAPA AWNVDAPQDS VSADAWWAQF GDPVMHQLVE SVLTGNLDVQ AAVERVKQAQ DLATQNRAAL LPELNASASA SDSRQNAPPP LGYVRQAGFG VTASWTPDVF GGERLAVLAA QAQVSGRQAA LNQVRLALAA NTAAAYIDLR WAQSQLQILN DNEQIRARAL KLTQARLHYG LSTQLDVARA QNQLEDLQAQ IPRVQSAVQH QLSLIAVYSG RTPESVDSLL LAHARDIPVP AQSVPQMLPS QALLQRPDVR TAYATVEQRA AEVGVSKAQR YPQFRLNLAD GLLASSYLGL PTLTDNLFSA ALNATSPIFN AGRITANIDA SESRMRESQL GLQQTMLQAL KEIEDNRSDL VSGAVQVQRL GGALDASDHA LRLSTELYKG GASSFLDVLA AQEAYLRDAE SLNQAKREHA LSAVALYRSL GGGWDVPDAV TAVSANN
NT Sequence
ATGAAACCGA TCCTTCTCAA AGTACTGGCG AGCGCCGCCC TGCTGACGCT CGCGGCGTGC GCGGTGCAGC CTGCAACGCA TGCGGATTTG CCGCAGACGG TGAAGACCGT CGCGCCCGCT GCGTGGAATG TCGATGCGCC GCAAGACAGC GTGAGCGCGG ATGCGTGGTG GGCGCAATTC GGCGACCCCG TCATGCATCA ACTGGTCGAA TCCGTGTTGA CCGGCAATCT CGACGTGCAG GCGGCCGTGG AACGCGTCAA GCAGGCGCAG GATCTGGCGA CGCAAAACCG CGCGGCCCTG CTGCCCGAGC TGAACGCGAG CGCGAGCGCG TCCGACTCGC GCCAGAACGC GCCGCCGCCG CTCGGTTATG TGCGTCAGGC CGGCTTCGGC GTAACGGCCA GCTGGACGCC CGACGTGTTC GGCGGCGAGC GTCTTGCGGT GCTCGCGGCG CAGGCGCAGG TATCGGGACG TCAGGCGGCT TTGAATCAGG TGCGCCTCGC GCTCGCGGCG AATACCGCTG CGGCGTACAT CGATCTGCGC TGGGCTCAAT CGCAATTGCA GATTCTGAAC GACAACGAGC AGATTCGCGC ACGCGCGTTG AAGCTCACGC AGGCGCGTCT GCATTACGGG CTGTCGACGC AACTGGACGT TGCCCGCGCG CAGAACCAGC TGGAGGATTT GCAGGCGCAG ATTCCGCGTG TGCAATCGGC GGTGCAGCAT CAGTTGAGTC TGATCGCGGT GTATTCGGGG CGCACGCCGG AAAGCGTGGA CAGTTTGCTG CTTGCCCATG CACGCGATAT TCCGGTGCCC GCGCAAAGCG TTCCGCAGAT GCTGCCTTCG CAAGCGCTCC TCCAGCGCCC CGACGTACGC ACCGCATACG CAACGGTCGA ACAGCGCGCG GCGGAAGTCG GCGTGTCGAA AGCGCAGCGC TATCCGCAAT TCAGGCTCAA TCTCGCCGAT GGTTTGCTGG CCTCGTCCTA CCTCGGCTTG CCGACACTGA CCGACAACCT CTTTAGCGCC GCCCTGAACG CAACGAGTCC GATTTTCAAC GCGGGCCGCA TCACCGCGAA TATCGACGCG AGCGAAAGCC GCATGCGCGA ATCGCAACTC GGTCTGCAGC AAACCATGCT GCAAGCGCTC AAGGAAATCG AGGACAACCG CAGCGATCTG GTGAGCGGCG CAGTGCAGGT CCAACGGCTT GGCGGCGCGC TCGATGCGTC GGACCACGCA CTGCGTCTGT CGACGGAACT GTACAAGGGC GGCGCGTCGA GTTTCCTGGA CGTGCTCGCG GCGCAGGAAG CCTATCTGCG CGACGCCGAG TCGTTGAACC AGGCCAAACG CGAGCACGCG TTGTCGGCGG TCGCTTTGTA CCGCTCGCTC GGCGGCGGCT GGGATGTACC TGACGCGGTC ACGGCGGTAT CGGCGAACAA C
Details for BuxeA.01673.c.A2.GE31993
HARVESTED ON: 4/5/2011
SEQUENCED ON: 4/11/2011
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MCAVQPATHA DLPQTVKTVA PAAWNVDAPQ DSVSADAWWA XFGDPVMHQL VESVLTGNLD VQAAVERVKQ AQDLATQNRA ALLPELNASA SASDSRQNAP PPLGYVRQAG FGVTASWTPD VFGGERLAVL AAQAQVSGRQ AALNQVXLAL AANTAAAYID LRWAQSQLQI LNDNEQIRAR ALKLTQARLH YGLSTQLDVA RAQNQLEDLQ AQIPRVQSAV QHQLSLIAVY SGRTPESVDS LLLAHARDIP VPAQSVPQML PSQALLQRPD VRTAYATVEQ RAAEVGVSKA QRYPQFRLNL ADGLLASSYL GLPTLTDNLF SAALNATSPI FNAGRITANI DASESRMRES QLGLQQTMLQ ALKEIEDNRS DLVSGAVQVQ RLGGALDASD HALRLSTELY KGGASSFLDV LAAQEAYLRD AESLNQAKRE HALSAVALYR SLGGGWDVPD AVTAVSA
Validated NT Sequence
tcgccgatac cgccgtgacc gcgtcaggta catcccagcc gccgccgagc gagcggtaca aagcgaccgc cgacaacgcg tgctcgcgtt tggcctggtt caacgactcg gcgtcgcgca gataggcttc ctgcgccgcg agcacgtcca ggaaactcga cgcgccgccc ttgtacagtt ccgtcgacag acgcagtgcg tggtccgacg catcgagcgc gccgccaagc cgttggacct gcactgcgcc gctcaccaga tcgctgcggt tgtcctcgat ttccttgagc gcttgcagca tggtttgctg cagaccgagt tgcgattcgc gcatgcggct ttcgctcgcg tcgatattcg cggtgatgcg gcccgcgttg aaaatcggac tcgttgcgtt cagggcggcg ctaaagaggt tgtcggtcag tgtcggcaag ccgaggtagg acgaggccag caaaccatcg gcgagattga gcctgaattg cggatagcgc tgcgctttcg acacgccgac ttccgccgcg cgctgttcga ccgttgcgta tgcggtgcgt acgtcggggc gctggaggag cgcttgcgaa ggcagcatct gcggaacgct ttgcgcgggc accggaatat cgcgtgcatg ggcaagcagc aaactgtcca cgctttccgg cgtgcgcccc gaatacaccg cgatcagact caactgatgc tgcaccgccg attgcacacg cggaatctgc gcctgcaaat cctccagctg gttctgcgcg cgggcaacgt ccagttgcgt cgacagcccg taatgcagac gcgcctgcgt gagcttcaac gcgcgtgcgc gaatctgctc gttgtcgttc agaatctgca attgcgattg agcccagcgc agatcgatgt acgccgcagc ggtattcgcc gcgagcgcga ggngcacctg attcaaagcc gcctgacgtc ccgatacctg cgcctgcgcc gcgagcaccg caagacgctc gccgccgaac acgtcgggcg tccagctggc cgttacgccg aagccggcct gacgcacata accgagcggc ggcggcgcgt tctggcgcga gtcggacgcg ctcgcgctcg cgttcagctc gggcagcagg gccgcgcggt tttgcgtcgc cagatcctgc gcctgcttga cgcgttccac ggccgcctgc acgtcgagat tgccggtcaa cacggattcg accagttgat gcatgacggg gtcgccgaan tgcgcccacc acgcatccgc gctcacgctg tcttgcggcg catcgacatt ccacgcagcg ggcgcgacgg tcttcaccgt ctgcggcaaa tccgcatgcg ttgcaggctg caccgcgcac atcgaaccag gacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCAVQPATH ADLPQTVKTV APAAWNVDAP QDSVSADAWW AQFGDPVMHQ LVESVLTGNL DVQAAVERVK QAQDLATQNR AALLPELNAS ASASDSRQNA PPPLGYVRQA GFGVTASWTP DVFGGERLAV LAAQAQVSGR QAALNQVRLA LAANTAAAYI DLRWAQSQLQ ILNDNEQIRA RALKLTQARL HYGLSTQLDV ARAQNQLEDL QAQIPRVQSA VQHQLSLIAV YSGRTPESVD SLLLAHARDI PVPAQSVPQM LPSQALLQRP DVRTAYATVE QRAAEVGVSK AQRYPQFRLN LADGLLASSY LGLPTLTDNL FSAALNATSP IFNAGRITAN IDASESRMRE SQLGLQQTML QALKEIEDNR SDLVSGAVQV QRLGGALDAS DHALRLSTEL YKGGASSFLD VLAAQEAYLR DAESLNQAKR EHALSAVALY RSLGGGWDVP DAVTAVSANN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgcgcggtg cagcctgcaa cgcatgcgga tttgccgcag acggtgaaga ccgtcgcgcc cgctgcgtgg aatgtcgatg cgccgcaaga cagcgtgagc gcggatgcgt ggtgggcgca attcggcgac cccgtcatgc atcaactggt cgaatccgtg ttgaccggca atctcgacgt gcaggcggcc gtggaacgcg tcaagcaggc gcaggatctg gcgacgcaaa accgcgcggc cctgctgccc gagctgaacg cgagcgcgag cgcgtccgac tcgcgccaga acgcgccgcc gccgctcggt tatgtgcgtc aggccggctt cggcgtaacg gccagctgga cgcccgacgt gttcggcggc gagcgtcttg cggtgctcgc ggcgcaggcg caggtatcgg gacgtcaggc ggctttgaat caggtgcgcc tcgcgctcgc ggcgaatacc gctgcggcgt acatcgatct gcgctgggct caatcgcaat tgcagattct gaacgacaac gagcagattc gcgcacgcgc gttgaagctc acgcaggcgc gtctgcatta cgggctgtcg acgcaactgg acgttgcccg cgcgcagaac cagctggagg atttgcaggc gcagattccg cgtgtgcaat cggcggtgca gcatcagttg agtctgatcg cggtgtattc ggggcgcacg ccggaaagcg tggacagttt gctgcttgcc catgcacgcg atattccggt gcccgcgcaa agcgttccgc agatgctgcc ttcgcaagcg ctcctccagc gccccgacgt acgcaccgca tacgcaacgg tcgaacagcg cgcggcggaa gtcggcgtgt cgaaagcgca gcgctatccg caattcaggc tcaatctcgc cgatggtttg ctggcctcgt cctacctcgg cttgccgaca ctgaccgaca acctctttag cgccgccctg aacgcaacga gtccgatttt caacgcgggc cgcatcaccg cgaatatcga cgcgagcgaa agccgcatgc gcgaatcgca actcggtctg cagcaaacca tgctgcaagc gctcaaggaa atcgaggaca accgcagcga tctggtgagc ggcgcagtgc aggtccaacg gcttggcggc gcgctcgatg cgtcggacca cgcactgcgt ctgtcgacgg aactgtacaa gggcggcgcg tcgagtttcc tggacgtgct cgcggcgcag gaagcctatc tgcgcgacgc cgagtcgttg aaccaggcca aacgcgagca cgcgttgtcg gcggtcgctt tgtaccgctc gctcggcggc ggctgggatg tacctgacgc ggtcacggcg gtatcggcga acaactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BuxeA.01673.c.A2.PW31909
PURIFICATION DATe: 5/20/2011
CONCENTRATION: 27.39mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MCAVQPATHA DLPQTVKTVA PAAWNVDAPQ DSVSADAWWA XFGDPVMHQL VESVLTGNLD VQAAVERVKQ AQDLATQNRA ALLPELNASA SASDSRQNAP PPLGYVRQAG FGVTASWTPD VFGGERLAVL AAQAQVSGRQ AALNQVXLAL AANTAAAYID LRWAQSQLQI LNDNEQIRAR ALKLTQARLH YGLSTQLDVA RAQNQLEDLQ AQIPRVQSAV QHQLSLIAVY SGRTPESVDS LLLAHARDIP VPAQSVPQML PSQALLQRPD VRTAYATVEQ RAAEVGVSKA QRYPQFRLNL ADGLLASSYL GLPTLTDNLF SAALNATSPI FNAGRITANI DASESRMRES QLGLQQTMLQ ALKEIEDNRS DLVSGAVQVQ RLGGALDASD HALRLSTELY KGGASSFLDV LAAQEAYLRD AESLNQAKRE HALSAVALYR SLGGGWDVPD AVTAVSA
Validated NT Sequence
tcgccgatac cgccgtgacc gcgtcaggta catcccagcc gccgccgagc gagcggtaca aagcgaccgc cgacaacgcg tgctcgcgtt tggcctggtt caacgactcg gcgtcgcgca gataggcttc ctgcgccgcg agcacgtcca ggaaactcga cgcgccgccc ttgtacagtt ccgtcgacag acgcagtgcg tggtccgacg catcgagcgc gccgccaagc cgttggacct gcactgcgcc gctcaccaga tcgctgcggt tgtcctcgat ttccttgagc gcttgcagca tggtttgctg cagaccgagt tgcgattcgc gcatgcggct ttcgctcgcg tcgatattcg cggtgatgcg gcccgcgttg aaaatcggac tcgttgcgtt cagggcggcg ctaaagaggt tgtcggtcag tgtcggcaag ccgaggtagg acgaggccag caaaccatcg gcgagattga gcctgaattg cggatagcgc tgcgctttcg acacgccgac ttccgccgcg cgctgttcga ccgttgcgta tgcggtgcgt acgtcggggc gctggaggag cgcttgcgaa ggcagcatct gcggaacgct ttgcgcgggc accggaatat cgcgtgcatg ggcaagcagc aaactgtcca cgctttccgg cgtgcgcccc gaatacaccg cgatcagact caactgatgc tgcaccgccg attgcacacg cggaatctgc gcctgcaaat cctccagctg gttctgcgcg cgggcaacgt ccagttgcgt cgacagcccg taatgcagac gcgcctgcgt gagcttcaac gcgcgtgcgc gaatctgctc gttgtcgttc agaatctgca attgcgattg agcccagcgc agatcgatgt acgccgcagc ggtattcgcc gcgagcgcga ggngcacctg attcaaagcc gcctgacgtc ccgatacctg cgcctgcgcc gcgagcaccg caagacgctc gccgccgaac acgtcgggcg tccagctggc cgttacgccg aagccggcct gacgcacata accgagcggc ggcggcgcgt tctggcgcga gtcggacgcg ctcgcgctcg cgttcagctc gggcagcagg gccgcgcggt tttgcgtcgc cagatcctgc gcctgcttga cgcgttccac ggccgcctgc acgtcgagat tgccggtcaa cacggattcg accagttgat gcatgacggg gtcgccgaan tgcgcccacc acgcatccgc gctcacgctg tcttgcggcg catcgacatt ccacgcagcg ggcgcgacgg tcttcaccgt ctgcggcaaa tccgcatgcg ttgcaggctg caccgcgcac atcgaaccag gacc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCAVQPATH ADLPQTVKTV APAAWNVDAP QDSVSADAWW AQFGDPVMHQ LVESVLTGNL DVQAAVERVK QAQDLATQNR AALLPELNAS ASASDSRQNA PPPLGYVRQA GFGVTASWTP DVFGGERLAV LAAQAQVSGR QAALNQVRLA LAANTAAAYI DLRWAQSQLQ ILNDNEQIRA RALKLTQARL HYGLSTQLDV ARAQNQLEDL QAQIPRVQSA VQHQLSLIAV YSGRTPESVD SLLLAHARDI PVPAQSVPQM LPSQALLQRP DVRTAYATVE QRAAEVGVSK AQRYPQFRLN LADGLLASSY LGLPTLTDNL FSAALNATSP IFNAGRITAN IDASESRMRE SQLGLQQTML QALKEIEDNR SDLVSGAVQV QRLGGALDAS DHALRLSTEL YKGGASSFLD VLAAQEAYLR DAESLNQAKR EHALSAVALY RSLGGGWDVP DAVTAVSANN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTGCGCGGTG CAGCCTGCAA CGCATGCGGA TTTGCCGCAG ACGGTGAAGA CCGTCGCGCC CGCTGCGTGG AATGTCGATG CGCCGCAAGA CAGCGTGAGC GCGGATGCGT GGTGGGCGCA ATTCGGCGAC CCCGTCATGC ATCAACTGGT CGAATCCGTG TTGACCGGCA ATCTCGACGT GCAGGCGGCC GTGGAACGCG TCAAGCAGGC GCAGGATCTG GCGACGCAAA ACCGCGCGGC CCTGCTGCCC GAGCTGAACG CGAGCGCGAG CGCGTCCGAC TCGCGCCAGA ACGCGCCGCC GCCGCTCGGT TATGTGCGTC AGGCCGGCTT CGGCGTAACG GCCAGCTGGA CGCCCGACGT GTTCGGCGGC GAGCGTCTTG CGGTGCTCGC GGCGCAGGCG CAGGTATCGG GACGTCAGGC GGCTTTGAAT CAGGTGCGCC TCGCGCTCGC GGCGAATACC GCTGCGGCGT ACATCGATCT GCGCTGGGCT CAATCGCAAT TGCAGATTCT GAACGACAAC GAGCAGATTC GCGCACGCGC GTTGAAGCTC ACGCAGGCGC GTCTGCATTA CGGGCTGTCG ACGCAACTGG ACGTTGCCCG CGCGCAGAAC CAGCTGGAGG ATTTGCAGGC GCAGATTCCG CGTGTGCAAT CGGCGGTGCA GCATCAGTTG AGTCTGATCG CGGTGTATTC GGGGCGCACG CCGGAAAGCG TGGACAGTTT GCTGCTTGCC CATGCACGCG ATATTCCGGT GCCCGCGCAA AGCGTTCCGC AGATGCTGCC TTCGCAAGCG CTCCTCCAGC GCCCCGACGT ACGCACCGCA TACGCAACGG TCGAACAGCG CGCGGCGGAA GTCGGCGTGT CGAAAGCGCA GCGCTATCCG CAATTCAGGC TCAATCTCGC CGATGGTTTG CTGGCCTCGT CCTACCTCGG CTTGCCGACA CTGACCGACA ACCTCTTTAG CGCCGCCCTG AACGCAACGA GTCCGATTTT CAACGCGGGC CGCATCACCG CGAATATCGA CGCGAGCGAA AGCCGCATGC GCGAATCGCA ACTCGGTCTG CAGCAAACCA TGCTGCAAGC GCTCAAGGAA ATCGAGGACA ACCGCAGCGA TCTGGTGAGC GGCGCAGTGC AGGTCCAACG GCTTGGCGGC GCGCTCGATG CGTCGGACCA CGCACTGCGT CTGTCGACGG AACTGTACAA GGGCGGCGCG TCGAGTTTCC TGGACGTGCT CGCGGCGCAG GAAGCCTATC TGCGCGACGC CGAGTCGTTG AACCAGGCCA AACGCGAGCA CGCGTTGTCG GCGGTCGCTT TGTACCGCTC GCTCGGCGGC GGCTGGGATG TACCTGACGC GGTCACGGCG GTATCGGCGA ACAACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA