BuxeA.01673.a

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuxeA.01673.a
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01673.a.A2.GE34887 Mature protein( BuxeA.01673.a ) 27 492
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.8896
RefSeq: YP_556357.1
UniProt: Q13FY2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASMSRIAAV KLPALLASVC ALAACSLAPD YHVPTAPVAA QYHTVGPWVN AQPADQLTRT DWWNIYNDPQ LDDLEQRLLA NNTDLAASYA HYRQAMAFVD QVSSNLFPRI SASAVPQRDR QSDTRPLRAG GPDYYNSATL GGEIDYDLDL WGRVRDSVAA GKDEAQATQA DLASAQLSLQ VQLADSYIRL RGLDQQTRLL TDTVAAYARA LELTQTLHGG GIVSGLDVSR ARTQLSSTKS QLSQNLAQRA LIEHAIAELI GASASEFSLA PKTALIALPV IPVGVPSTLL QRRPDIAAAE RRVAEANAKI GVARAAYFPS ITLSLQGGVQ SAAYAGLVSA PNIFWAIGPQ LVQYVFDGGL RRAQLDSAKA ATDEAGARYR GVVLSAFQQI EDNLSLLQDL GTALDQQRDA DDSAQHAVDL ALNRYRQGTV GYLDVVQAQT AALDAQRGVL DLQTRLLSAN VQLVKALGGG WSSQELATAA HAPALVAARG SP
NT Sequence
ATGGCTAGTA TGAGCAGGAT CGCCGCGGTA AAGCTTCCCG CGCTGCTGGC CAGCGTCTGC GCGCTCGCGG CGTGTTCCCT TGCGCCGGAC TACCATGTGC CGACGGCGCC GGTCGCCGCG CAATACCACA CAGTGGGACC GTGGGTCAAC GCGCAGCCCG CGGACCAGTT AACGCGCACC GACTGGTGGA ATATCTATAA CGACCCGCAA CTCGACGATC TCGAACAGCG TCTGCTCGCG AACAATACCG ATCTTGCCGC GTCCTACGCC CATTACCGGC AGGCCATGGC ATTCGTCGAT CAGGTCTCGT CGAATCTGTT CCCGCGGATC AGCGCGAGCG CGGTTCCGCA GCGTGACCGT CAGTCCGACA CGCGGCCATT GCGCGCCGGC GGGCCCGATT ACTACAACTC GGCCACGCTC GGCGGCGAAA TCGATTACGA CCTCGATCTA TGGGGCCGCG TGCGCGATTC CGTGGCGGCG GGCAAGGATG AAGCGCAGGC CACCCAGGCC GATCTTGCCT CCGCGCAACT GAGCCTGCAG GTTCAACTCG CCGACAGTTA TATCCGCCTG CGCGGGCTGG ACCAGCAAAC CCGGTTGCTG ACCGACACCG TCGCGGCGTA CGCGCGCGCA TTGGAACTGA CGCAAACGCT GCATGGCGGA GGGATTGTTT CGGGGCTCGA CGTATCGCGC GCGAGGACGC AGTTGTCGTC GACGAAATCG CAGCTGTCGC AGAACCTTGC ACAGCGCGCG CTGATCGAGC ACGCGATCGC CGAGCTGATT GGCGCGTCCG CATCCGAATT CAGTCTGGCG CCGAAAACCG CATTGATCGC ATTGCCGGTC ATTCCGGTCG GCGTGCCGTC GACGTTGTTG CAACGACGTC CGGATATCGC GGCCGCCGAG CGTCGCGTGG CGGAGGCAAA TGCGAAGATC GGCGTGGCAC GCGCCGCTTA TTTCCCGTCC ATTACGCTGA GCTTGCAAGG CGGCGTCCAG AGCGCCGCGT ATGCCGGGTT GGTCAGCGCA CCGAACATTT TCTGGGCGAT CGGTCCGCAA CTCGTGCAAT ACGTTTTCGA CGGCGGCTTG CGCCGCGCGC AACTCGACTC GGCGAAAGCG GCCACCGACG AAGCGGGCGC GCGTTATCGC GGCGTCGTGC TGTCGGCGTT CCAGCAAATC GAAGACAATC TTTCGCTGCT CCAGGACCTC GGCACCGCGC TTGACCAGCA GCGCGATGCC GACGATTCGG CGCAGCACGC CGTCGACCTG GCCTTGAACC GCTACCGGCA GGGCACGGTC GGCTATCTCG ACGTGGTACA GGCGCAGACG GCGGCGCTCG ATGCACAACG CGGCGTGCTC GATCTCCAGA CCCGCTTGCT GAGCGCGAAC GTGCAACTGG TCAAGGCGCT CGGCGGCGGC TGGTCGAGCC AGGAACTCGC GACCGCCGCG CATGCTCCAG CCCTGGTCGC AGCCCGCGGT TCACCA
Details for BuxeA.01673.a.A2.GE34887
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 61
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLAPDYHVP TAPVAAQYHT VGPWVNAQPA DQLTRTDWWN IYNDPQLDDL EQRLLANNTD LAASYAHYRQ AMAFVDQVSS NLFPRISASA VPQRDRQSDT RPLRAGGPDY YNSATLGGEI DYDLDLWGRV RDSVAAGKDE AQATQADLAS AQLSLQVQLA DSYIRLRGLD QQTRLLTDTV AAYARALELT QTLHGGGIVS GLDVSRARTQ LSSTKSQLSQ NLAQRALIEH AIAELIGASA SEFSLAPKTA XIALPVXPVG VPSTLXQRRP DXAAAXXXV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcttg cgccggacta ccatgtgccg acggcgccgg tcgccgcgca ataccacaca gtgggaccgt gggtcaacgc gcagcccgcg gaccagttaa cgcgcaccga ctggtggaat atctataacg acccgcaact cgacgatctc gaacagcgtc tgctcgcgaa caataccgat cttgccgcgt cctacgccca ttaccggcag gccatggcat tcgtcgatca ggtctcgtcg aatctgttcc cgcggatcag cgcgagcgcg gttccgcagc gtgaccgtca gtccgacacg cggccattgc gcgccggcgg gcccgattac tacaactcgg ccacgctcgg cggcgaaatc gattacgacc tcgatctatg gggccgcgtg cgcgattccg tggcggcggg caaggatgaa gcgcaggcca cccaggccga tcttgcctcc gcgcaactga gcctgcaggt tcaactcgcc gacagttata tccgcctgcg cgggctggac cagcaaaccc ggttgctgac cgacaccgtc gcggcgtacg cgcgcgcatt ggaactgacg caaacgctgc atggcggagg gattgtttcg gggctcgacg tatcgcgcgc gaggacgcag ttgtcgtcga cgaaatcgca gctgtcgcag aaccttgcac agcgcgcgct gatcgagcac gcgatcgccg agctgattgg cgcgtccgca tccgaattca gtctggcgcc gaaaaccgca ntgatcgcat tgccggtcan tccngtcggc gtgccgtcga cgttgntgca acgacgtccg gatntcgcgg ccgccnagcn tcncgtggcg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLAPDYHVP TAPVAAQYHT VGPWVNAQPA DQLTRTDWWN IYNDPQLDDL EQRLLANNTD LAASYAHYRQ AMAFVDQVSS NLFPRISASA VPQRDRQSDT RPLRAGGPDY YNSATLGGEI DYDLDLWGRV RDSVAAGKDE AQATQADLAS AQLSLQVQLA DSYIRLRGLD QQTRLLTDTV AAYARALELT QTLHGGGIVS GLDVSRARTQ LSSTKSQLSQ NLAQRALIEH AIAELIGASA SEFSLAPKTA LIALPVIPVG VPSTLLQRRP DIAAAERRVA EANAKIGVAR AAYFPSITLS LQGGVQSAAY AGLVSAPNIF WAIGPQLVQY VFDGGLRRAQ LDSAKAATDE AGARYRGVVL SAFQQIEDNL SLLQDLGTAL DQQRDADDSA QHAVDLALNR YRQGTVGYLD VVQAQTAALD AQRGVLDLQT RLLSANVQLV KALGGGWSSQ ELATAAHAPA LVAARGSP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcttgcgccg gactaccatg tgccgacggc gccggtcgcc gcgcaatacc acacagtggg accgtgggtc aacgcgcagc ccgcggacca gttaacgcgc accgactggt ggaatatcta taacgacccg caactcgacg atctcgaaca gcgtctgctc gcgaacaata ccgatcttgc cgcgtcctac gcccattacc ggcaggccat ggcattcgtc gatcaggtct cgtcgaatct gttcccgcgg atcagcgcga gcgcggttcc gcagcgtgac cgtcagtccg acacgcggcc attgcgcgcc ggcgggcccg attactacaa ctcggccacg ctcggcggcg aaatcgatta cgacctcgat ctatggggcc gcgtgcgcga ttccgtggcg gcgggcaagg atgaagcgca ggccacccag gccgatcttg cctccgcgca actgagcctg caggttcaac tcgccgacag ttatatccgc ctgcgcgggc tggaccagca aacccggttg ctgaccgaca ccgtcgcggc gtacgcgcgc gcattggaac tgacgcaaac gctgcatggc ggagggattg tttcggggct cgacgtatcg cgcgcgagga cgcagttgtc gtcgacgaaa tcgcagctgt cgcagaacct tgcacagcgc gcgctgatcg agcacgcgat cgccgagctg attggcgcgt ccgcatccga attcagtctg gcgccgaaaa ccgcattgat cgcattgccg gtcattccgg tcggcgtgcc gtcgacgttg ttgcaacgac gtccggatat cgcggccgcc gagcgtcgcg tggcggaggc aaatgcgaag atcggcgtgg cacgcgccgc ttatttcccg tccattacgc tgagcttgca aggcggcgtc cagagcgccg cgtatgccgg gttggtcagc gcaccgaaca ttttctgggc gatcggtccg caactcgtgc aatacgtttt cgacggcggc ttgcgccgcg cgcaactcga ctcggcgaaa gcggccaccg acgaagcggg cgcgcgttat cgcggcgtcg tgctgtcggc gttccagcaa atcgaagaca atctttcgct gctccaggac ctcggcaccg cgcttgacca gcagcgcgat gccgacgatt cggcgcagca cgccgtcgac ctggccttga accgctaccg gcagggcacg gtcggctatc tcgacgtggt acaggcgcag acggcggcgc tcgatgcaca acgcggcgtg ctcgatctcc agacccgctt gctgagcgcg aacgtgcaac tggtcaaggc gctcggcggc ggctggtcga gccaggaact cgcgaccgcc gcgcatgctc cagccctggt cgcagcccgc ggttcaccat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa