SSGCID: Target Page

BuxeA.01669.a

PAS/PAC sensor hybrid histidine kinase

CENTER ID:	BuxeA.01669.a
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.01669.a.A1.GE34896	Full length( BuxeA.01669.a )		1	657

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.2518
RefSeq:	YP_558992.1
UniProt:	Q13Y99

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNQLDVPSAG QNVEPANVPA PTRMNPAADV DYWALMQAIE DYAIFLLDAT GRIVSCNTGA LKQAGYTERE IIGQHFSCFY TEDAVARDWP AYELQQASLT GRFEDEGWRV RKNGTTFWSN VVITAIRNDA GILTGFAKIT RDLTARRDYL EALRQSEERF RLLVDSVKDY AIFMLDPQGN VVSWNEGAAR IKGYTREEIV GRHFSTFYPP EDAAAGKPAR ELAIARQIGH VEDEGWRVRK DGTTFWANVN ITAVYDESKR LCGFAKVTRD LTERRQREEL ERSGERMRQF LATLAHELRN PLAPVRNAIG VMQLETGISP TLARSRDLID RQVTHLTRLV DDLLDVGRIM SNKVELRVGR VDLAEVVARG IEAARPFTDA HEQRVVAHMP LEPISVRGDM TRLVQVLQNL LHNASKFSPV GSVVDIVARI DFHMAVLEVR DAGCGIALRS LDRIFDLFAQ EKEAQTIGEG GLGIGLTLCK SLVEMHGGSI VASSEGPGSG STFTLSLPLA AAPSNAAGDE ANAANGQVAP LRILLVDDNR DSADSLAMLL ELKGHEVRIA YEGEQALQVA PRFAPHLAVI DIAMPKMDGY ATIAALREMP GLAHTMYAAM TGFGHATDRE RSRDAGFHAH LVKPVELALF DTLLAEVEAR RSGRTSG

NT Sequence

ATGAACCAAC TCGATGTCCC CTCCGCCGGC CAGAACGTCG AGCCTGCCAA CGTCCCGGCG CCGACCCGCA TGAACCCGGC GGCGGACGTC GACTACTGGG CGCTGATGCA GGCGATCGAA GACTATGCAA TCTTCCTGCT CGACGCGACC GGCCGGATCG TCAGCTGCAA TACCGGCGCA CTGAAGCAGG CGGGTTACAC GGAGAGGGAG ATCATCGGCC AGCACTTTTC GTGCTTCTAT ACCGAGGATG CCGTGGCACG CGACTGGCCC GCGTACGAAT TGCAGCAGGC TTCGCTGACC GGCCGCTTCG AGGACGAAGG CTGGCGGGTT CGCAAGAACG GCACCACCTT CTGGTCGAAT GTCGTGATCA CGGCGATCCG CAACGACGCG GGCATTCTCA CCGGCTTCGC CAAGATCACG CGCGATCTGA CCGCGCGGCG CGACTATCTG GAGGCGCTGC GGCAAAGCGA AGAGCGTTTC CGTTTGCTGG TCGACAGTGT CAAGGACTAC GCGATATTCA TGCTCGACCC GCAAGGCAAC GTGGTGAGCT GGAATGAAGG CGCCGCGCGC ATCAAAGGCT ATACGCGCGA AGAGATCGTC GGCCGGCATT TCTCGACCTT CTATCCGCCG GAAGACGCGG CGGCGGGCAA GCCCGCGCGC GAACTGGCGA TTGCGCGGCA GATCGGCCAT GTCGAGGACG AAGGCTGGCG GGTGCGCAAG GACGGCACGA CTTTCTGGGC CAACGTCAAC ATCACCGCGG TGTATGACGA ATCGAAGCGG CTATGCGGCT TCGCCAAGGT CACGCGCGAT CTGACCGAGC GGCGTCAGCG TGAAGAACTG GAGCGTTCGG GTGAACGCAT GCGCCAGTTT CTTGCGACCC TCGCGCATGA ATTGCGCAAT CCGCTCGCGC CGGTGCGCAA TGCGATCGGC GTGATGCAGC TCGAAACCGG CATCAGCCCG ACGCTCGCGC GTTCGCGCGA TCTGATCGAC CGCCAGGTCA CGCACCTCAC AAGGTTGGTC GATGATCTGC TCGACGTTGG CCGGATCATG TCCAACAAGG TCGAACTGCG CGTTGGCCGT GTCGATCTGG CCGAGGTGGT GGCGCGCGGG ATCGAGGCGG CCCGGCCGTT CACGGACGCG CACGAGCAGC GCGTCGTCGC GCATATGCCG CTCGAGCCGA TATCCGTGCG CGGCGACATG ACGCGTCTCG TTCAGGTGCT GCAAAATCTC CTGCACAACG CGTCCAAATT CTCGCCGGTG GGCAGTGTTG TCGACATCGT GGCGCGCATC GATTTTCACA TGGCCGTGCT CGAAGTGCGC GACGCCGGTT GCGGCATTGC GCTGCGCTCG CTCGACAGGA TCTTCGACCT GTTCGCTCAG GAAAAAGAGG CGCAGACGAT CGGCGAAGGC GGTCTGGGTA TCGGCCTCAC GTTGTGCAAG TCGCTCGTCG AGATGCACGG CGGCAGCATT GTCGCGAGCA GTGAAGGGCC AGGCAGCGGC AGTACGTTCA CGCTGAGCCT GCCGCTCGCC GCCGCACCGT CCAACGCCGC CGGTGACGAG GCGAACGCGG CCAACGGCCA GGTCGCGCCG CTGCGCATTC TGCTGGTCGA CGACAACCGC GATTCGGCCG ACAGTCTGGC CATGCTGCTG GAACTCAAAG GCCACGAGGT GCGCATTGCC TACGAGGGCG AGCAGGCGCT GCAGGTCGCG CCGCGCTTCG CGCCGCATCT CGCCGTGATC GACATTGCAA TGCCGAAAAT GGACGGCTAC GCGACCATCG CGGCGCTGCG CGAAATGCCC GGGCTGGCCC ACACGATGTA CGCCGCCATG ACCGGCTTCG GCCATGCAAC CGATCGCGAG CGCAGCCGCG ACGCCGGTTT CCATGCGCAT CTGGTGAAGC CGGTGGAACT CGCGCTATTC GATACGCTGC TGGCCGAAGT TGAAGCGCGG CGCAGCGGGC GCACTTCAGG C

Details for BuxeA.01669.a.A1.GE34896

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	74kDa
OBSERVED MW:	74kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	42

Validated AA Sequence

Validated NT Sequence

gaaggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg aaccaactcg atgtcccctc cgccggccag aacgtcgagc ctgccaacgt cccggcgccg acccgcatga acccggcggc ggacgtcgac tactgggcgc tgatgcaggc gatcgaagac tatgcaatct tcctgctcga cgcgaccggc cggatcgtca gctgcaatac cggcgcactg aagcaggcgg gttacacgga gagggagatc atcggccagc acttttcgtg cttctatacc gaggatgccg tggcacgcga ctggcccgcg tacgaattgc agcaggcttc gctgaccggc cgcttcgagg acgaaggctg gcgggttcgc aagaacggca ccaccttctg gtcgaatgtc gtgatcacgg cgatccgcaa cgacgcgggc attctcaccg gcttcgccaa gatcacgcgc gatctgaccg cgcggcgcga ctatctggag gcgctgcggc aaagcgaaga gcgtttccgt ttgctggtcg acagtgtcaa ggactacgcg atattcatgc tcgacccgca aggcaacgtg gtgagctgga atgaaggcgc cgcgcgcatc aaaggctata cgcgcgaaga gatcgtcggc cggcatttct cgaccttcta tccgccggaa gangcggcgg cgggcaagcc cgcgcgcgaa ctggcgattg cgcggcagat cggccatgtc gaggacgaan gctggcgggt gcgcaaggac ggcacgactt tctgggccaa cgtcaacatc accgcggtgt atgacgaatc gaancggcta tgcggcttcg ccaag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNQLDVPSA GQNVEPANVP APTRMNPAAD VDYWALMQAI EDYAIFLLDA TGRIVSCNTG ALKQAGYTER EIIGQHFSCF YTEDAVARDW PAYELQQASL TGRFEDEGWR VRKNGTTFWS NVVITAIRND AGILTGFAKI TRDLTARRDY LEALRQSEER FRLLVDSVKD YAIFMLDPQG NVVSWNEGAA RIKGYTREEI VGRHFSTFYP PEDAAAGKPA RELAIARQIG HVEDEGWRVR KDGTTFWANV NITAVYDESK RLCGFAKVTR DLTERRQREE LERSGERMRQ FLATLAHELR NPLAPVRNAI GVMQLETGIS PTLARSRDLI DRQVTHLTRL VDDLLDVGRI MSNKVELRVG RVDLAEVVAR GIEAARPFTD AHEQRVVAHM PLEPISVRGD MTRLVQVLQN LLHNASKFSP VGSVVDIVAR IDFHMAVLEV RDAGCGIALR SLDRIFDLFA QEKEAQTIGE GGLGIGLTLC KSLVEMHGGS IVASSEGPGS GSTFTLSLPL AAAPSNAAGD EANAANGQVA PLRILLVDDN RDSADSLAML LELKGHEVRI AYEGEQALQV APRFAPHLAV IDIAMPKMDG YATIAALREM PGLAHTMYAA MTGFGHATDR ERSRDAGFHA HLVKPVELAL FDTLLAEVEA RRSGRTSG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaccaactc gatgtcccct ccgccggcca gaacgtcgag cctgccaacg tcccggcgcc gacccgcatg aacccggcgg cggacgtcga ctactgggcg ctgatgcagg cgatcgaaga ctatgcaatc ttcctgctcg acgcgaccgg ccggatcgtc agctgcaata ccggcgcact gaagcaggcg ggttacacgg agagggagat catcggccag cacttttcgt gcttctatac cgaggatgcc gtggcacgcg actggcccgc gtacgaattg cagcaggctt cgctgaccgg ccgcttcgag gacgaaggct ggcgggttcg caagaacggc accaccttct ggtcgaatgt cgtgatcacg gcgatccgca acgacgcggg cattctcacc ggcttcgcca agatcacgcg cgatctgacc gcgcggcgcg actatctgga ggcgctgcgg caaagcgaag agcgtttccg tttgctggtc gacagtgtca aggactacgc gatattcatg ctcgacccgc aaggcaacgt ggtgagctgg aatgaaggcg ccgcgcgcat caaaggctat acgcgcgaag agatcgtcgg ccggcatttc tcgaccttct atccgccgga agacgcggcg gcgggcaagc ccgcgcgcga actggcgatt gcgcggcaga tcggccatgt cgaggacgaa ggctggcggg tgcgcaagga cggcacgact ttctgggcca acgtcaacat caccgcggtg tatgacgaat cgaagcggct atgcggcttc gccaaggtca cgcgcgatct gaccgagcgg cgtcagcgtg aagaactgga gcgttcgggt gaacgcatgc gccagtttct tgcgaccctc gcgcatgaat tgcgcaatcc gctcgcgccg gtgcgcaatg cgatcggcgt gatgcagctc gaaaccggca tcagcccgac gctcgcgcgt tcgcgcgatc tgatcgaccg ccaggtcacg cacctcacaa ggttggtcga tgatctgctc gacgttggcc ggatcatgtc caacaaggtc gaactgcgcg ttggccgtgt cgatctggcc gaggtggtgg cgcgcgggat cgaggcggcc cggccgttca cggacgcgca cgagcagcgc gtcgtcgcgc atatgccgct cgagccgata tccgtgcgcg gcgacatgac gcgtctcgtt caggtgctgc aaaatctcct gcacaacgcg tccaaattct cgccggtggg cagtgttgtc gacatcgtgg cgcgcatcga ttttcacatg gccgtgctcg aagtgcgcga cgccggttgc ggcattgcgc tgcgctcgct cgacaggatc ttcgacctgt tcgctcagga aaaagaggcg cagacgatcg gcgaaggcgg tctgggtatc ggcctcacgt tgtgcaagtc gctcgtcgag atgcacggcg gcagcattgt cgcgagcagt gaagggccag gcagcggcag tacgttcacg ctgagcctgc cgctcgccgc cgcaccgtcc aacgccgccg gtgacgaggc gaacgcggcc aacggccagg tcgcgccgct gcgcattctg ctggtcgacg acaaccgcga ttcggccgac agtctggcca tgctgctgga actcaaaggc cacgaggtgc gcattgccta cgagggcgag caggcgctgc aggtcgcgcc gcgcttcgcg ccgcatctcg ccgtgatcga cattgcaatg ccgaaaatgg acggctacgc gaccatcgcg gcgctgcgcg aaatgcccgg gctggcccac acgatgtacg ccgccatgac cggcttcggc catgcaaccg atcgcgagcg cagccgcgac gccggtttcc atgcgcatct ggtgaagccg gtggaactcg cgctattcga tacgctgctg gccgaagttg aagcgcggcg cagcgggcgc acttcaggct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa