BuxeA.01668.d

Histidine kinase

CENTER ID: BuxeA.01668.d
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01668.d.A1.GE31989 Full length( BuxeA.01668.d ) 1 552
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01668.d.A1.PS01088 Full length( BuxeA.01668.d ) 1 552

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.5091
RefSeq: YP_552733.1
UniProt: Q13RA6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEQRVLILAP FGRDADVIAE VLRKDDRMCV ACHDADALTT ALDAGAGAAL IAEEALAGDH AMRLFNWLEQ QPAWSDFPFT LLAAPRVGHR SERGLEVLER LGNVVVLERP LNSETLRRAV ASSLRARARQ YETRRHLAER IQAQDALVQL NDSLESRIAE RTHELASANN RLMMEIHERA KVQAVLVQSQ KMEALGQLTG GIAHDFNNLL NVIMVNAELI ARVSSDERLR GMAATVKRAT ERGAKLTGQL LTFSRNSNLD LKAVDVVTLL QGMRDIITVS LGSSIRYTNE FDGAELWTEA DANQLELAIL NLAINARDAM PAGGQLGIRV KEREAPDQTL EAGPYVVIEV ADTGSGVPPE VVSRVFDPFF TTKPIGKGTG LGLSQVYGIA TQAGGTARLF SEEGVGTTVE IWLPLRERVV PQAEATSNAE ANAVGEKRVL VVEDDGEVRA MLVESLRMLG YTVTEAADGR AGLNRLQDDK PDLLMVDFAM PGMNGIDVIA EARKLREDLP VILATGYADV DISGLAVKRC TILRKPFQLD DLARTVRLGL AA
NT Sequence
ATGGAACAAC GCGTTCTTAT CCTCGCGCCG TTTGGCCGCG ACGCCGATGT GATCGCAGAA GTACTGCGCA AAGACGACCG CATGTGCGTG GCATGCCACG ATGCCGACGC GCTCACCACC GCGCTGGATG CCGGCGCCGG CGCCGCGCTG ATTGCCGAAG AAGCGCTGGC GGGCGACCAT GCAATGCGCC TGTTCAACTG GCTCGAACAG CAGCCGGCAT GGTCCGACTT CCCGTTCACG CTGCTCGCCG CGCCGCGCGT CGGACATCGC TCGGAGCGCG GGCTGGAAGT GCTCGAGCGG CTCGGCAACG TGGTCGTACT CGAACGTCCG CTGAATTCGG AAACGCTGCG GCGCGCGGTG GCGTCGTCAT TACGGGCACG GGCGCGCCAA TACGAGACGC GCCGCCATCT CGCCGAACGG ATCCAGGCGC AGGATGCGCT GGTGCAATTG AACGACTCGC TGGAGAGCCG CATTGCAGAG CGCACCCACG AGCTTGCTTC CGCCAACAAC CGGCTGATGA TGGAGATTCA CGAGCGCGCC AAAGTGCAGG CCGTGCTGGT GCAATCGCAG AAAATGGAAG CGCTCGGGCA ACTCACCGGC GGCATCGCGC ACGACTTCAA CAATCTGCTG AACGTCATCA TGGTCAACGC GGAGCTGATC GCTCGCGTGA GCAGCGACGA GCGCCTTCGC GGCATGGCCG CCACCGTCAA ACGCGCGACC GAACGCGGCG CGAAACTGAC AGGACAGTTG CTCACTTTCT CGCGCAACAG CAACCTCGAC CTGAAAGCGG TCGACGTGGT GACGTTGCTG CAAGGCATGC GCGACATCAT CACGGTATCG CTCGGTTCGA GCATTCGCTA CACCAACGAG TTCGACGGCG CGGAGCTGTG GACCGAAGCC GACGCCAATC AGCTCGAACT GGCCATCCTC AATCTCGCCA TCAACGCGCG CGACGCCATG CCGGCCGGCG GCCAGCTCGG GATTCGCGTG AAAGAGCGCG AAGCGCCGGA TCAGACGCTC GAAGCCGGCC CCTATGTGGT GATCGAAGTC GCCGATACCG GTTCGGGCGT GCCGCCCGAA GTCGTGTCGC GCGTGTTCGA TCCGTTCTTC ACCACCAAGC CGATCGGCAA GGGAACCGGA CTCGGCCTGA GCCAGGTCTA CGGGATCGCG ACGCAGGCCG GCGGCACCGC GCGTCTCTTC AGCGAGGAAG GCGTGGGCAC CACCGTCGAG ATCTGGCTAC CGTTGCGCGA ACGCGTGGTG CCGCAAGCCG AAGCGACGTC GAATGCGGAA GCGAACGCGG TCGGCGAAAA ACGCGTGCTG GTGGTCGAAG ACGACGGCGA GGTGCGCGCC ATGCTCGTCG AGTCGCTAAG AATGCTCGGC TACACCGTGA CCGAAGCCGC CGACGGACGC GCCGGACTGA ACCGTTTGCA GGACGACAAA CCGGACCTGC TGATGGTGGA CTTCGCCATG CCCGGCATGA ACGGGATCGA CGTGATCGCG GAGGCGCGCA AACTGCGCGA GGATCTACCG GTGATCCTCG CAACCGGCTA TGCGGACGTG GATATTTCCG GACTGGCGGT GAAGCGCTGC ACGATCCTGC GCAAACCGTT TCAGCTCGAT GACCTGGCAC GTACGGTGAG GCTTGGGCTC GCAGCG
Details for BuxeA.01668.d.A1.GE31989
HARVESTED ON: 4/5/2011
SEQUENCED ON: 4/11/2011
EXPECTED MW: 62kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 35
PERCENT COVERAGE: 27
Validated AA Sequence
MRLFNWLEQX PXWSDFPFTL LAAPRVGHRS ERGLEVLXRL GNVVVLERXL NXXXQXXXXX XXLRAXAPXP DXXXXXXXDX XXXXXXXXER XXGXPHXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX HXXXXAXLXX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
cngccatgtt tacnnnnact cnnnngnnna nnnnnannna annanaantc gnannnnnna nnnttnnaan annggtcctg gttcnangga acancncnnn cntnnnntcg cgccgnttgg ccgcnannnc natgtgatcc ccnannnnct gcgcanannc gaccgcnngn gnnngccctg cnannnnnnn nnnncnnnnn nnnnnntnnn ttnnnggcnc cngnnccgcg ntgantgncn aanaagcgct ggcgggcgac catgcaatgc gcctgttcaa ctggctcgaa cagcanccgg natggtccga cttcccgttc acgctgctcg ccgcgccgcg cgtcggacat cgctcggagc gcgggctgga agtgctcnag cggctcggca acgtggtcgt actcgaacgt cnnctgaatt nnnanncnca gnnnnncgng nngnnntnnn cnttacgggc acnggcgcca atnccagacg nnnncncnnn nngcnnaann gatcnnngnn cnngangnnc tnnnnnnnnt gaacgantcn ctgganagcc gcattgnnna ncgnncnnna nctngnttnc nncnnnnnng ncngannnnn nancnnnnnn gngnanggnn tgntnntncn ntnncannaa nnnnnncncn ncnnntcacn ngnngcntng cgcncnactt cnnnnnnngn nnnnnnnntn ntgntnnnnn nnnnnnnnan nnnnnnnnaa nnnnnacnac nnnntc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEQRVLILA PFGRDADVIA EVLRKDDRMC VACHDADALT TALDAGAGAA LIAEEALAGD HAMRLFNWLE QQPAWSDFPF TLLAAPRVGH RSERGLEVLE RLGNVVVLER PLNSETLRRA VASSLRARAR QYETRRHLAE RIQAQDALVQ LNDSLESRIA ERTHELASAN NRLMMEIHER AKVQAVLVQS QKMEALGQLT GGIAHDFNNL LNVIMVNAEL IARVSSDERL RGMAATVKRA TERGAKLTGQ LLTFSRNSNL DLKAVDVVTL LQGMRDIITV SLGSSIRYTN EFDGAELWTE ADANQLELAI LNLAINARDA MPAGGQLGIR VKEREAPDQT LEAGPYVVIE VADTGSGVPP EVVSRVFDPF FTTKPIGKGT GLGLSQVYGI ATQAGGTARL FSEEGVGTTV EIWLPLRERV VPQAEATSNA EANAVGEKRV LVVEDDGEVR AMLVESLRML GYTVTEAADG RAGLNRLQDD KPDLLMVDFA MPGMNGIDVI AEARKLREDL PVILATGYAD VDISGLAVKR CTILRKPFQL DDLARTVRLG LAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaacaacgc gttcttatcc tcgcgccgtt tggccgcgac gccgatgtga tcgcagaagt actgcgcaaa gacgaccgca tgtgcgtggc atgccacgat gccgacgcgc tcaccaccgc gctggatgcc ggcgccggcg ccgcgctgat tgccgaagaa gcgctggcgg gcgaccatgc aatgcgcctg ttcaactggc tcgaacagca gccggcatgg tccgacttcc cgttcacgct gctcgccgcg ccgcgcgtcg gacatcgctc ggagcgcggg ctggaagtgc tcgagcggct cggcaacgtg gtcgtactcg aacgtccgct gaattcggaa acgctgcggc gcgcggtggc gtcgtcatta cgggcacggg cgcgccaata cgagacgcgc cgccatctcg ccgaacggat ccaggcgcag gatgcgctgg tgcaattgaa cgactcgctg gagagccgca ttgcagagcg cacccacgag cttgcttccg ccaacaaccg gctgatgatg gagattcacg agcgcgccaa agtgcaggcc gtgctggtgc aatcgcagaa aatggaagcg ctcgggcaac tcaccggcgg catcgcgcac gacttcaaca atctgctgaa cgtcatcatg gtcaacgcgg agctgatcgc tcgcgtgagc agcgacgagc gccttcgcgg catggccgcc accgtcaaac gcgcgaccga acgcggcgcg aaactgacag gacagttgct cactttctcg cgcaacagca acctcgacct gaaagcggtc gacgtggtga cgttgctgca aggcatgcgc gacatcatca cggtatcgct cggttcgagc attcgctaca ccaacgagtt cgacggcgcg gagctgtgga ccgaagccga cgccaatcag ctcgaactgg ccatcctcaa tctcgccatc aacgcgcgcg acgccatgcc ggccggcggc cagctcggga ttcgcgtgaa agagcgcgaa gcgccggatc agacgctcga agccggcccc tatgtggtga tcgaagtcgc cgataccggt tcgggcgtgc cgcccgaagt cgtgtcgcgc gtgttcgatc cgttcttcac caccaagccg atcggcaagg gaaccggact cggcctgagc caggtctacg ggatcgcgac gcaggccggc ggcaccgcgc gtctcttcag cgaggaaggc gtgggcacca ccgtcgagat ctggctaccg ttgcgcgaac gcgtggtgcc gcaagccgaa gcgacgtcga atgcggaagc gaacgcggtc ggcgaaaaac gcgtgctggt ggtcgaagac gacggcgagg tgcgcgccat gctcgtcgag tcgctaagaa tgctcggcta caccgtgacc gaagccgccg acggacgcgc cggactgaac cgtttgcagg acgacaaacc ggacctgctg atggtggact tcgccatgcc cggcatgaac gggatcgacg tgatcgcgga ggcgcgcaaa ctgcgcgagg atctaccggt gatcctcgca accggctatg cggacgtgga tatttccgga ctggcggtga agcgctgcac gatcctgcgc aaaccgtttc agctcgatga cctggcacgt acggtgaggc ttgggctcgc agcgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BuxeA.01668.d.A1.PS01088
PURIFICATION DATe: 5/20/2011
CONCENTRATION: 36.1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 35
PERCENT COVERAGE: 27
Protocol Notes
notes unavailable
Validated AA Sequence
MRLFNWLEQX PXWSDFPFTL LAAPRVGHRS ERGLEVLXRL GNVVVLERXL NXXXQXXXXX XXLRAXAPXP DXXXXXXXDX XXXXXXXXER XXGXPHXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX HXXXXAXLXX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
cngccatgtt tacnnnnact cnnnngnnna nnnnnannna annanaantc gnannnnnna nnnttnnaan annggtcctg gttcnangga acancncnnn cntnnnntcg cgccgnttgg ccgcnannnc natgtgatcc ccnannnnct gcgcanannc gaccgcnngn gnnngccctg cnannnnnnn nnnncnnnnn nnnnnntnnn ttnnnggcnc cngnnccgcg ntgantgncn aanaagcgct ggcgggcgac catgcaatgc gcctgttcaa ctggctcgaa cagcanccgg natggtccga cttcccgttc acgctgctcg ccgcgccgcg cgtcggacat cgctcggagc gcgggctgga agtgctcnag cggctcggca acgtggtcgt actcgaacgt cnnctgaatt nnnanncnca gnnnnncgng nngnnntnnn cnttacgggc acnggcgcca atnccagacg nnnncncnnn nngcnnaann gatcnnngnn cnngangnnc tnnnnnnnnt gaacgantcn ctgganagcc gcattgnnna ncgnncnnna nctngnttnc nncnnnnnng ncngannnnn nancnnnnnn gngnanggnn tgntnntncn ntnncannaa nnnnnncncn ncnnntcacn ngnngcntng cgcncnactt cnnnnnnngn nnnnnnnntn ntgntnnnnn nnnnnnnnan nnnnnnnnaa nnnnnacnac nnnntc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEQRVLILA PFGRDADVIA EVLRKDDRMC VACHDADALT TALDAGAGAA LIAEEALAGD HAMRLFNWLE QQPAWSDFPF TLLAAPRVGH RSERGLEVLE RLGNVVVLER PLNSETLRRA VASSLRARAR QYETRRHLAE RIQAQDALVQ LNDSLESRIA ERTHELASAN NRLMMEIHER AKVQAVLVQS QKMEALGQLT GGIAHDFNNL LNVIMVNAEL IARVSSDERL RGMAATVKRA TERGAKLTGQ LLTFSRNSNL DLKAVDVVTL LQGMRDIITV SLGSSIRYTN EFDGAELWTE ADANQLELAI LNLAINARDA MPAGGQLGIR VKEREAPDQT LEAGPYVVIE VADTGSGVPP EVVSRVFDPF FTTKPIGKGT GLGLSQVYGI ATQAGGTARL FSEEGVGTTV EIWLPLRERV VPQAEATSNA EANAVGEKRV LVVEDDGEVR AMLVESLRML GYTVTEAADG RAGLNRLQDD KPDLLMVDFA MPGMNGIDVI AEARKLREDL PVILATGYAD VDISGLAVKR CTILRKPFQL DDLARTVRLG LAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAACAACGC GTTCTTATCC TCGCGCCGTT TGGCCGCGAC GCCGATGTGA TCGCAGAAGT ACTGCGCAAA GACGACCGCA TGTGCGTGGC ATGCCACGAT GCCGACGCGC TCACCACCGC GCTGGATGCC GGCGCCGGCG CCGCGCTGAT TGCCGAAGAA GCGCTGGCGG GCGACCATGC AATGCGCCTG TTCAACTGGC TCGAACAGCA GCCGGCATGG TCCGACTTCC CGTTCACGCT GCTCGCCGCG CCGCGCGTCG GACATCGCTC GGAGCGCGGG CTGGAAGTGC TCGAGCGGCT CGGCAACGTG GTCGTACTCG AACGTCCGCT GAATTCGGAA ACGCTGCGGC GCGCGGTGGC GTCGTCATTA CGGGCACGGG CGCGCCAATA CGAGACGCGC CGCCATCTCG CCGAACGGAT CCAGGCGCAG GATGCGCTGG TGCAATTGAA CGACTCGCTG GAGAGCCGCA TTGCAGAGCG CACCCACGAG CTTGCTTCCG CCAACAACCG GCTGATGATG GAGATTCACG AGCGCGCCAA AGTGCAGGCC GTGCTGGTGC AATCGCAGAA AATGGAAGCG CTCGGGCAAC TCACCGGCGG CATCGCGCAC GACTTCAACA ATCTGCTGAA CGTCATCATG GTCAACGCGG AGCTGATCGC TCGCGTGAGC AGCGACGAGC GCCTTCGCGG CATGGCCGCC ACCGTCAAAC GCGCGACCGA ACGCGGCGCG AAACTGACAG GACAGTTGCT CACTTTCTCG CGCAACAGCA ACCTCGACCT GAAAGCGGTC GACGTGGTGA CGTTGCTGCA AGGCATGCGC GACATCATCA CGGTATCGCT CGGTTCGAGC ATTCGCTACA CCAACGAGTT CGACGGCGCG GAGCTGTGGA CCGAAGCCGA CGCCAATCAG CTCGAACTGG CCATCCTCAA TCTCGCCATC AACGCGCGCG ACGCCATGCC GGCCGGCGGC CAGCTCGGGA TTCGCGTGAA AGAGCGCGAA GCGCCGGATC AGACGCTCGA AGCCGGCCCC TATGTGGTGA TCGAAGTCGC CGATACCGGT TCGGGCGTGC CGCCCGAAGT CGTGTCGCGC GTGTTCGATC CGTTCTTCAC CACCAAGCCG ATCGGCAAGG GAACCGGACT CGGCCTGAGC CAGGTCTACG GGATCGCGAC GCAGGCCGGC GGCACCGCGC GTCTCTTCAG CGAGGAAGGC GTGGGCACCA CCGTCGAGAT CTGGCTACCG TTGCGCGAAC GCGTGGTGCC GCAAGCCGAA GCGACGTCGA ATGCGGAAGC GAACGCGGTC GGCGAAAAAC GCGTGCTGGT GGTCGAAGAC GACGGCGAGG TGCGCGCCAT GCTCGTCGAG TCGCTAAGAA TGCTCGGCTA CACCGTGACC GAAGCCGCCG ACGGACGCGC CGGACTGAAC CGTTTGCAGG ACGACAAACC GGACCTGCTG ATGGTGGACT TCGCCATGCC CGGCATGAAC GGGATCGACG TGATCGCGGA GGCGCGCAAA CTGCGCGAGG ATCTACCGGT GATCCTCGCA ACCGGCTATG CGGACGTGGA TATTTCCGGA CTGGCGGTGA AGCGCTGCAC GATCCTGCGC AAACCGTTTC AGCTCGATGA CCTGGCACGT ACGGTGAGGC TTGGGCTCGC AGCGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA