BuxeA.01668.a

Histidine kinase

CENTER ID: BuxeA.01668.a
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01668.a.A1.GE31986 Full length( BuxeA.01668.a ) 1 288
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.8303
RefSeq: YP_555793.1
UniProt: Q13HJ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVHPTSFRW PTSAHGARHA DERDEIIEPA VRQPTVVAFN ASRTVGDHAS ASKLKNQVLA MVAHELRGPL TPLQLATQLM RRASADRPEV LRSLDMIDRQ IAQIARLAQD LMDATRIDRN VLRISRDHLD VVGFLAAPLA AAALATAKRG QTFTLQFADR TLRVEGDAVR LAQAIDNLLH NAAKYTPDHG CITVNVHSDG KDLIVSVTDN GLGISRALLP HIFDLFAQSS RTIAASAGGL GVGLAVVKAV AESHGGTVSA TSAGPGAGSE FTLRLPIVIE RRGADEPA
NT Sequence
ATGTCAGTCC ATCCAACGTC TTTCCGGTGG CCTACTAGCG CTCACGGCGC GCGCCATGCG GATGAGAGGG ATGAAATTAT CGAACCCGCT GTGCGACAGC CTACCGTCGT GGCCTTCAAT GCGTCCCGTA CGGTCGGCGA CCATGCCAGC GCATCGAAAC TGAAGAATCA GGTTCTGGCT ATGGTGGCGC ATGAGCTTCG CGGACCGCTC ACTCCGCTGC AACTCGCCAC TCAGCTCATG CGCCGGGCGT CGGCGGACCG ACCGGAAGTG CTTCGGTCGC TTGACATGAT CGATCGGCAA ATCGCACAGA TAGCCCGGCT CGCGCAGGAT TTGATGGACG CTACCCGGAT CGACCGGAAC GTCCTTCGCA TAAGCCGGGA CCACCTCGAC GTGGTCGGGT TTCTGGCCGC CCCACTCGCC GCCGCTGCAT TGGCGACGGC CAAACGCGGC CAGACATTCA CGCTGCAGTT CGCCGACCGG ACGCTACGCG TTGAAGGCGA CGCGGTCCGT CTTGCACAGG CCATCGACAA CCTCCTGCAT AACGCAGCGA AATACACACC CGACCATGGT TGCATCACGG TGAACGTACA TTCGGACGGA AAGGACCTCA TTGTCTCCGT CACGGACAAT GGTCTGGGCA TATCACGAGC ACTTCTGCCA CATATTTTCG ACCTCTTCGC TCAATCGAGC AGAACGATTG CGGCCAGTGC TGGCGGTTTG GGAGTGGGTC TTGCCGTCGT GAAAGCCGTC GCCGAATCGC ACGGCGGAAC CGTGTCGGCG ACCAGTGCGG GGCCCGGTGC CGGCAGTGAA TTCACGCTCC GGCTGCCCAT CGTGATCGAA AGGCGCGGGG CTGACGAGCC TGCC
Details for BuxeA.01668.a.A1.GE31986
HARVESTED ON: 4/5/2011
SEQUENCED ON: 4/11/2011
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMSVHSTS FRWPTSAHGA RHADERDEII EPAVRQPTVV AFNASRTVGD HASASKLKNQ VLAMVAHELR GPLTPLQLAT QLMRRASADR PEVLRSLDMI DRQIAQIARL AQDLMDATRI DRNVLRISRD HLDVVGFLAA PLAAAALATA KRGQTFTLQF ADRTLRVEGD AVRLAQAIDN LLHNAAKYTP DHGCITVNVH SDGKDLIVSV TDNGLGISRA LLPHIFDLFA QSSRTIAASA GGLGVGLAVV KAVAESHGGT VSATSAGPGA GSEFTLRLPI VIERXGADEP A
Validated NT Sequence
gcnnnnnnnc nnngnngnnn nntcaccntc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg tcagtccatt caacgtcttt ccggtggcct actagcgctc acggcgcgcg ccatgcggat gagagggatg aaattatcga acccgctgtg cgacagccta ccgtcgtggc cttcaatgcg tcccgtacgg tcggcgacca tgccagcgca tcgaaactga agaatcaggt tctggctatg gtggcgcatg agcttcgcgg accgctcact ccgctgcaac tcgccactca gctcatgcgc cgggcgtcgg cggaccgacc ggaagtgctt cggtcgcttg acatgatcga tcggcaaatc gcacagatag cccggctcgc gcaggatttg atggacgcta cccggatcga ccggaacgtc cttcgcataa gccgggacca cctcgacgtg gtcgggtttc tggccgcccc actcgccgcc gctgcattgg cgacggccaa acgcggccag acattcacgc tgcagttcgc cgaccggacg ctacgcgttg aaggcgacgc ggtccgtctt gcacaggcca tcgacaacct cctgcataac gcagcgaaat acacacccga ccatggttgc atcacggtga acgtacattc ggacggaaag gacctcattg tctccgtcac ggacaatggt ctgggcatat cacgagcact tctgccacat attttcgacc tcttcgctca atcgagcaga acgattgcgg ccagtgctgg cggtttggga gtgggtcttg ccgtcgtgaa agccgtcgcc gaatcgcacg gcggaaccgt gtcggcgacc agtgcggggc ccggtgccgg cagtgaattc acgctccggc tgcccatcgt gatcgaaagg nncggggctg acgagcctgc ctaataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccnn ctgctannna agcccgaaan nagctgantt ggctgctgcn nnnnnnnaaa nnnnnnnnnn na
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVHPTSFR WPTSAHGARH ADERDEIIEP AVRQPTVVAF NASRTVGDHA SASKLKNQVL AMVAHELRGP LTPLQLATQL MRRASADRPE VLRSLDMIDR QIAQIARLAQ DLMDATRIDR NVLRISRDHL DVVGFLAAPL AAAALATAKR GQTFTLQFAD RTLRVEGDAV RLAQAIDNLL HNAAKYTPDH GCITVNVHSD GKDLIVSVTD NGLGISRALL PHIFDLFAQS SRTIAASAGG LGVGLAVVKA VAESHGGTVS ATSAGPGAGS EFTLRLPIVI ERRGADEPA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcagtccat ccaacgtctt tccggtggcc tactagcgct cacggcgcgc gccatgcgga tgagagggat gaaattatcg aacccgctgt gcgacagcct accgtcgtgg ccttcaatgc gtcccgtacg gtcggcgacc atgccagcgc atcgaaactg aagaatcagg ttctggctat ggtggcgcat gagcttcgcg gaccgctcac tccgctgcaa ctcgccactc agctcatgcg ccgggcgtcg gcggaccgac cggaagtgct tcggtcgctt gacatgatcg atcggcaaat cgcacagata gcccggctcg cgcaggattt gatggacgct acccggatcg accggaacgt ccttcgcata agccgggacc acctcgacgt ggtcgggttt ctggccgccc cactcgccgc cgctgcattg gcgacggcca aacgcggcca gacattcacg ctgcagttcg ccgaccggac gctacgcgtt gaaggcgacg cggtccgtct tgcacaggcc atcgacaacc tcctgcataa cgcagcgaaa tacacacccg accatggttg catcacggtg aacgtacatt cggacggaaa ggacctcatt gtctccgtca cggacaatgg tctgggcata tcacgagcac ttctgccaca tattttcgac ctcttcgctc aatcgagcag aacgattgcg gccagtgctg gcggtttggg agtgggtctt gccgtcgtga aagccgtcgc cgaatcgcac ggcggaaccg tgtcggcgac cagtgcgggg cccggtgccg gcagtgaatt cacgctccgg ctgcccatcg tgatcgaaag gcgcggggct gacgagcctg cctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa