BuxeA.01666.f

Putative nitrogen regulation, histidine kinase, protein NR(II)

CENTER ID: BuxeA.01666.f
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.01666.f.A1.GE31983 Full length( BuxeA.01666.f ) 1 380
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.3042
RefSeq: YP_559483.1
UniProt: Q13WV8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVLKNLIKAR KTHEPTLSDD AQLVSSGLLP GFEALPTVVL VLEKRTLRVA FANPSAESML ELSRRQLTQM AWPHIFSNAD ELVATISAIA AHRFHATHLD AVLERPGHEP LHVHAIVGFL ESAQDYVLLE LFENERHLRT DREERINDLT AVNKQLIRNL AHEIKNPLGG IRGAAQLLEF ELGERQRDEL REYTQVIIKE SDRLQTLVDR LLEPHRHPHI VGDVNIHEVC ERVRQVILAE FPRGLTIERD YDVSVPDLRG DKEQLIQALL NIVRNAAEAL RERISQGDAR IELRTRVARK ITVSKRLCKL ALDLHITDNG PGIPEEIRDR IFYPLVSGRE DGSGLGLTLA QTFVQQHDGL IEVDSRPGHT EFQILLPLDC
NT Sequence
ATGGTTCTTA AGAATCTGAT CAAGGCAAGG AAAACGCACG AGCCGACACT CTCGGACGAC GCCCAACTCG TCAGCTCCGG TTTGCTGCCG GGCTTCGAGG CGTTGCCGAC AGTCGTGCTG GTGCTCGAAA AGCGCACGCT GCGCGTCGCG TTTGCGAACC CGTCGGCGGA GTCGATGCTG GAGCTCTCGC GCCGGCAACT GACGCAAATG GCGTGGCCGC ACATCTTCTC GAATGCCGAC GAACTGGTCG CGACCATTTC CGCGATCGCC GCTCACCGCT TTCATGCCAC GCATCTGGAC GCCGTGCTCG AACGTCCGGG CCACGAACCG CTGCATGTGC ACGCGATCGT GGGTTTTCTG GAAAGCGCGC AAGACTATGT GCTGCTCGAA CTGTTCGAGA ACGAACGGCA TTTACGCACC GACCGCGAGG AGCGCATCAA CGACCTCACG GCGGTCAACA AGCAACTGAT CCGCAATCTC GCGCACGAGA TCAAGAACCC GCTCGGCGGG ATTCGCGGCG CGGCGCAACT GCTCGAGTTC GAACTCGGCG AGCGTCAGCG TGACGAACTG CGCGAGTACA CGCAGGTGAT CATCAAGGAA TCGGACCGGC TGCAAACGCT GGTTGACCGT TTGCTCGAAC CGCACCGTCA TCCGCATATC GTCGGCGACG TGAATATTCA CGAAGTGTGC GAACGCGTGC GCCAGGTGAT TCTCGCGGAG TTTCCGCGCG GCCTCACCAT CGAGCGCGAT TACGACGTGA GCGTGCCGGA CCTGCGCGGC GACAAGGAGC AACTGATCCA GGCGCTCCTG AACATCGTGC GCAACGCGGC CGAAGCGTTG CGCGAACGTA TTTCGCAAGG CGATGCGCGG ATCGAATTGC GCACCCGCGT TGCCCGCAAG ATTACGGTGT CGAAACGCTT ATGTAAGCTG GCATTGGACT TGCATATCAC AGACAACGGC CCGGGCATTC CCGAAGAAAT CCGTGACCGC ATTTTCTATC CGCTCGTGTC GGGGCGCGAG GACGGTAGCG GTCTCGGACT GACGCTTGCG CAGACTTTCG TGCAACAACA CGACGGTCTG ATCGAAGTGG ATAGCCGCCC GGGTCACACC GAGTTTCAGA TTCTGCTGCC GCTCGACTGC
Details for BuxeA.01666.f.A1.GE31983
HARVESTED ON: 4/5/2011
SEQUENCED ON: 4/11/2011
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MVLKNLIKAR KTHEPTLSDD AQLVSSGLLP GFEALPTVVL VLEKRTLRVA FANPSAESML ELSRRQLTQM AWPHIFSNAD ELVATISAIA AHRFHATHLD AVLERPGHEP LHVHAIVGFL ESAQDYVLLE LFENERHLRT DREERINDLT AVNKQLIRNL AHEIKNPLGG IRGAAQLLEF ELGERQRDEL REYTQVIIKE SDRLQTLVDR LLEPHRHPHI VGDVNIHEVC ERVRQVILAE FPRGLTIERD YDVSVPDLRG DKEQLIQALL NIVRNAAEAL RERISQGDAR IELRTRVARK ITVSKRLCKL ALDLHITDNG PGIPEEIRDR IFYPLVSGRE DGSGLGLTLA QTFVQQHDGL IEVDSRPGHT EFQILLPL
Validated NT Sequence
cgagcggcag cagaatctga aactcggtgt gacccgggcg gctatccact tcgatcagac cgtcgtgttg ttgcacgaaa gtctgcgcaa gcgtcagtcc gagaccgcta ccgtcctcgc gccccgacac gagcggatag aaaatgcggt cacggatttc ttcgggaatg cccgggccgt tgtctgtgat atgcaagtcc aatgccagct tacataagcg tttcgacacc gtaatcttgc gggcaacgcg ggtgcgcaat tcgatccgcg catcgccttg cgaaatacgt tcgcgcaacg cttcggccgc gttgcgcacg atgttcagga gcgcctggat cagttgctcc ttgtcgccgc gcaggtccgg cacgctcacg tcgtaatcgc gctcgatggt gaggccgcgc ggaaactccg cgagaatcac ctggcgcacg cgttcgcaca cttcgtgaat attcacgtcg ccgacgatat gcggatgacg gtgcggttcg agcaaacggt caaccagcgt ttgcagccgg tccgattcct tgatgatcac ctgcgtgtac tcgcgcagtt cgtcacgctg acgctcgccg agttcgaact cgagcagttg cgccgcgccg cgaatcccgc cgagcgggtt cttgatctcg tgcgcgagat tgcggatcag ttgcttgttg accgccgtga ggtcgttgat gcgctcctcg cggtcggtgc gtaaatgccg ttcgttctcg aacagttcga gcagcacata gtcttgcgcg ctttccagaa aacccacgat cgcgtgcaca tgcagcggtt cgtggcccgg acgttcgagc acggcgtcca gatgcgtggc atgaaagcgg tgagcggcga tcgcggaaat ggtcgcgacc agttcgtcgg cattcgagaa gatgtgcggc cacgccattt gcgtcagttg ccggcgcgag agctccagca tcgactccgc cgacgggttc gcaaacgcga cgcgcagcgt gcgcttttcg agcaccagca cgactgtcgg caacgcctcg aagcccggca gcaaaccgga gctgacgagt tgggcgtcgt ccgagagtgt cggctcgtgc gttttccttg ccttgatcag attcttaaga accatcgaac caggaccctg ggtctgagct tccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLKNLIKA RKTHEPTLSD DAQLVSSGLL PGFEALPTVV LVLEKRTLRV AFANPSAESM LELSRRQLTQ MAWPHIFSNA DELVATISAI AAHRFHATHL DAVLERPGHE PLHVHAIVGF LESAQDYVLL ELFENERHLR TDREERINDL TAVNKQLIRN LAHEIKNPLG GIRGAAQLLE FELGERQRDE LREYTQVIIK ESDRLQTLVD RLLEPHRHPH IVGDVNIHEV CERVRQVILA EFPRGLTIER DYDVSVPDLR GDKEQLIQAL LNIVRNAAEA LRERISQGDA RIELRTRVAR KITVSKRLCK LALDLHITDN GPGIPEEIRD RIFYPLVSGR EDGSGLGLTL AQTFVQQHDG LIEVDSRPGH TEFQILLPLD C
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttcttaag aatctgatca aggcaaggaa aacgcacgag ccgacactct cggacgacgc ccaactcgtc agctccggtt tgctgccggg cttcgaggcg ttgccgacag tcgtgctggt gctcgaaaag cgcacgctgc gcgtcgcgtt tgcgaacccg tcggcggagt cgatgctgga gctctcgcgc cggcaactga cgcaaatggc gtggccgcac atcttctcga atgccgacga actggtcgcg accatttccg cgatcgccgc tcaccgcttt catgccacgc atctggacgc cgtgctcgaa cgtccgggcc acgaaccgct gcatgtgcac gcgatcgtgg gttttctgga aagcgcgcaa gactatgtgc tgctcgaact gttcgagaac gaacggcatt tacgcaccga ccgcgaggag cgcatcaacg acctcacggc ggtcaacaag caactgatcc gcaatctcgc gcacgagatc aagaacccgc tcggcgggat tcgcggcgcg gcgcaactgc tcgagttcga actcggcgag cgtcagcgtg acgaactgcg cgagtacacg caggtgatca tcaaggaatc ggaccggctg caaacgctgg ttgaccgttt gctcgaaccg caccgtcatc cgcatatcgt cggcgacgtg aatattcacg aagtgtgcga acgcgtgcgc caggtgattc tcgcggagtt tccgcgcggc ctcaccatcg agcgcgatta cgacgtgagc gtgccggacc tgcgcggcga caaggagcaa ctgatccagg cgctcctgaa catcgtgcgc aacgcggccg aagcgttgcg cgaacgtatt tcgcaaggcg atgcgcggat cgaattgcgc acccgcgttg cccgcaagat tacggtgtcg aaacgcttat gtaagctggc attggacttg catatcacag acaacggccc gggcattccc gaagaaatcc gtgaccgcat tttctatccg ctcgtgtcgg ggcgcgagga cggtagcggt ctcggactga cgcttgcgca gactttcgtg caacaacacg acggtctgat cgaagtggat agccgcccgg gtcacaccga gtttcagatt ctgctgccgc tcgactgcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a