SSGCID: Target Page

BuxeA.01664.a

Histidine kinase

CENTER ID:	BuxeA.01664.a
ORGANISM:	Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.01664.a.A1.GE31974	Full length( BuxeA.01664.a )		1	268

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuxeA.01664.a.A1.PS01107	Full length( BuxeA.01664.a )		1	268

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|266265.5.peg.4820
RefSeq:	YP_552475.1
UniProt:	Q13S14

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTSSTGNAG EKPVPSGFAV SGRTALLRAE TALFMRDHVL SLVSHDLRGP LNAIHSWAYV LERKLDANDP NAQRAVTGIR NGVDQQVKLL ETIVDATRAE TKSLVLAYAP FSLHPLLEET VEEVRSGLGR ARGVEVTVDS QLATEQLNGD RERLAAALWL MLMFAVETGA EGATVTLVTK ADTNAWHASV GYEANGTALD DPALPHVLEA FARKQAREPR EAKRIAWVLA LCKRVAEAHG GSFEQVDATG SDTATLALRV PLSATAAK

NT Sequence

GTGACAACGT CTTCAACCGG AAATGCTGGC GAAAAGCCGG TGCCTTCCGG CTTCGCCGTA TCTGGACGCA CCGCGCTTCT GCGCGCGGAA ACCGCACTGT TCATGCGTGA CCATGTGCTG TCGCTGGTGT CGCACGACCT GCGTGGTCCG TTGAATGCCA TTCATAGCTG GGCGTACGTG CTGGAGCGCA AACTCGACGC GAACGACCCC AACGCGCAGC GCGCCGTCAC CGGTATTCGC AATGGCGTGG ATCAGCAGGT CAAGCTACTC GAGACGATTG TCGACGCCAC GCGCGCCGAG ACCAAATCAC TGGTGCTTGC CTACGCGCCC TTTTCCCTGC ATCCGTTACT CGAAGAAACG GTGGAAGAAG TCCGCTCGGG TCTGGGTCGC GCGCGCGGCG TGGAAGTGAC GGTCGACTCG CAACTGGCCA CCGAGCAACT CAACGGCGAC CGGGAGCGCC TCGCCGCCGC GCTATGGCTG ATGCTCATGT TCGCGGTAGA AACGGGCGCG GAAGGCGCCA CGGTCACCCT GGTCACGAAA GCGGATACGA ACGCATGGCA TGCTAGCGTC GGGTACGAGG CCAACGGCAC GGCGTTGGAC GATCCCGCCT TGCCGCATGT ACTCGAAGCG TTTGCCCGCA AGCAGGCCCG CGAGCCACGC GAAGCGAAGC GGATCGCGTG GGTGCTGGCG CTGTGCAAGC GCGTTGCCGA AGCGCACGGC GGCAGTTTCG AGCAGGTCGA CGCAACGGGC AGCGACACGG CCACGCTCGC CCTGCGCGTG CCGCTCAGCG CAACGGCTGC GAAA

Details for BuxeA.01664.a.A1.GE31974

HARVESTED ON:	4/5/2011
SEQUENCED ON:	4/11/2011
EXPECTED MW:	31kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMVTTSST GNAGEKPVPS GFAVSGRTAL LRAETALFMR DHVLSLVSHD LRGPLNAIHS WAYVLERKLD ANDPNAQRAV TGIRNGVDQQ VKLLETIVDA TRAETKSLVL AYAPFSLHPL LEETVEEVRS GLGRARGVEV TVDSQLATEQ LNGDRERLAA ALWLMLMFAV ETGAEGATVT LVTKADTNAW HASVGYEANG TALDDPALPH VLEAFARKQA REPREAKRIA WVLALCKRVA EAHGGSFEQV DATGSDTATL ALRVPLSATA AK

Validated NT Sequence

ttattatttc gcagccgttg cgctgagcgg cacgcgcagg gcgagcgtgg ccgtgtcgct gcccgttgcg tcgacctgct cgaaactgcc gccgtgcgct tcggcaacgc gcttgcacag cgccagcacc cacgcgatcc gcttcgcttc gcgtggctcg cgggcctgct tgcgggcaaa cgcttcgagt acatgcggca aggcgggatc gtccaacgcc gtgccgttgg cctcgtaccc gacgctagca tgccatgcgt tcgtatccgc tttcgtgacc agggtgaccg tggcgccttc cgcgcccgtt tctaccgcga acatgagcat cagccatagc gcggcggcga ggcgctcccg gtcgccgttg agttgctcgg tggccagttg cgagtcgacc gtcacttcca cgccgcgcgc gcgacccaga cccgagcgga cttcttccac cgtttcttcg agtaacggat gcagggaaaa gggcgcgtag gcaagcacca gtgatttggt ctcggcgcgc gtggcgtcga caatcgtctc gagtagcttg acctgctgat ccacgccatt gcgaataccg gtgacggcgc gctgcgcgtt ggggtcgttc gcgtcgagtt tgcgctccag cacgtacgcc cagctatgaa tggcattcaa cggaccacgc aggtcgtgcg acaccagcga cagcacatgg tcacgcatga acagtgcggt ttccgcgcgc agaagcgcgg tgcgtccaga tacggcgaag ccggaaggca ccggcttttc gccagcattt ccggttgaag acgttgtcac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVTTSSTGN AGEKPVPSGF AVSGRTALLR AETALFMRDH VLSLVSHDLR GPLNAIHSWA YVLERKLDAN DPNAQRAVTG IRNGVDQQVK LLETIVDATR AETKSLVLAY APFSLHPLLE ETVEEVRSGL GRARGVEVTV DSQLATEQLN GDRERLAAAL WLMLMFAVET GAEGATVTLV TKADTNAWHA SVGYEANGTA LDDPALPHVL EAFARKQARE PREAKRIAWV LALCKRVAEA HGGSFEQVDA TGSDTATLAL RVPLSATAAK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacaacg tcttcaaccg gaaatgctgg cgaaaagccg gtgccttccg gcttcgccgt atctggacgc accgcgcttc tgcgcgcgga aaccgcactg ttcatgcgtg accatgtgct gtcgctggtg tcgcacgacc tgcgtggtcc gttgaatgcc attcatagct gggcgtacgt gctggagcgc aaactcgacg cgaacgaccc caacgcgcag cgcgccgtca ccggtattcg caatggcgtg gatcagcagg tcaagctact cgagacgatt gtcgacgcca cgcgcgccga gaccaaatca ctggtgcttg cctacgcgcc cttttccctg catccgttac tcgaagaaac ggtggaagaa gtccgctcgg gtctgggtcg cgcgcgcggc gtggaagtga cggtcgactc gcaactggcc accgagcaac tcaacggcga ccgggagcgc ctcgccgccg cgctatggct gatgctcatg ttcgcggtag aaacgggcgc ggaaggcgcc acggtcaccc tggtcacgaa agcggatacg aacgcatggc atgctagcgt cgggtacgag gccaacggca cggcgttgga cgatcccgcc ttgccgcatg tactcgaagc gtttgcccgc aagcaggccc gcgagccacg cgaagcgaag cggatcgcgt gggtgctggc gctgtgcaag cgcgttgccg aagcgcacgg cggcagtttc gagcaggtcg acgcaacggg cagcgacacg gccacgctcg ccctgcgcgt gccgctcagc gcaacggctg cgaaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BuxeA.01664.a.A1.PS01107

PURIFICATION DATe:	6/3/2011
CONCENTRATION:	58.1mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACAACG TCTTCAACCG GAAATGCTGG CGAAAAGCCG GTGCCTTCCG GCTTCGCCGT ATCTGGACGC ACCGCGCTTC TGCGCGCGGA AACCGCACTG TTCATGCGTG ACCATGTGCT GTCGCTGGTG TCGCACGACC TGCGTGGTCC GTTGAATGCC ATTCATAGCT GGGCGTACGT GCTGGAGCGC AAACTCGACG CGAACGACCC CAACGCGCAG CGCGCCGTCA CCGGTATTCG CAATGGCGTG GATCAGCAGG TCAAGCTACT CGAGACGATT GTCGACGCCA CGCGCGCCGA GACCAAATCA CTGGTGCTTG CCTACGCGCC CTTTTCCCTG CATCCGTTAC TCGAAGAAAC GGTGGAAGAA GTCCGCTCGG GTCTGGGTCG CGCGCGCGGC GTGGAAGTGA CGGTCGACTC GCAACTGGCC ACCGAGCAAC TCAACGGCGA CCGGGAGCGC CTCGCCGCCG CGCTATGGCT GATGCTCATG TTCGCGGTAG AAACGGGCGC GGAAGGCGCC ACGGTCACCC TGGTCACGAA AGCGGATACG AACGCATGGC ATGCTAGCGT CGGGTACGAG GCCAACGGCA CGGCGTTGGA CGATCCCGCC TTGCCGCATG TACTCGAAGC GTTTGCCCGC AAGCAGGCCC GCGAGCCACG CGAAGCGAAG CGGATCGCGT GGGTGCTGGC GCTGTGCAAG CGCGTTGCCG AAGCGCACGG CGGCAGTTTC GAGCAGGTCG ACGCAACGGG CAGCGACACG GCCACGCTCG CCCTGCGCGT GCCGCTCAGC GCAACGGCTG CGAAATAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA