BuxeA.00863.a

Response regulator receiver sensor signal transduction histidine kinase

CENTER ID: BuxeA.00863.a
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00863.a.A1.GE31969 Full length( BuxeA.00863.a ) 1 375
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00863.a.A1.PS01108 Full length( BuxeA.00863.a ) 1 375

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.6537
RefSeq: YP_554087.1
UniProt: Q13MF2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNSPVNILI VDDIVHNITA LEALLARPDL AVLVADSGTA ALDLLLKHEV ALAILDVNMP GMNGFELAAL MRGSPRTSHV PIIFLTATAQ DASRTFRGYE AGAVDFLYKP FDPRILQSKV DVFVQLEQQK RQLAAQLVTT RQMLEANEML MAVLSHDLRT PLGAVLASAE YLMRTAADEQ SVTVAARVKN SSLRMARMVD QLLNLARLQG GRLPLQPRSI ELATLCRSVI DEFASRENGK QIVFASRGNT TGAWDTDLVW QAVSNLVSNA LHHGAPGGDI GVEVDGEAVD SVRLKVANRG TIPPEVFPHL FKAFGPNNSG ARSREGLGLG LHIVQEIARM HGGSVGVHSE EATGTVFTIE LPRMVTFQRG SFTRK
NT Sequence
ATGACGAATT CACCCGTGAA CATTCTGATC GTCGACGACA TCGTCCACAA CATCACGGCG CTCGAGGCCT TGCTGGCGCG GCCGGATCTT GCCGTGCTGG TGGCCGATTC GGGCACCGCG GCGCTCGACC TGCTGCTCAA GCACGAAGTC GCGCTGGCGA TTCTCGACGT CAACATGCCG GGCATGAACG GCTTCGAACT GGCCGCGTTG ATGCGTGGCA GCCCGCGCAC CTCGCACGTG CCGATCATTT TTCTGACCGC GACCGCGCAG GACGCGTCGC GCACGTTTCG CGGCTACGAG GCCGGCGCGG TCGACTTCCT GTACAAGCCG TTCGATCCGC GCATCCTGCA ATCCAAGGTG GATGTGTTCG TGCAGCTCGA ACAGCAGAAG CGCCAGCTCG CGGCGCAGCT CGTCACCACG CGGCAGATGC TGGAAGCCAA CGAGATGCTG ATGGCGGTGC TGAGTCATGA TCTGCGCACG CCGCTGGGCG CCGTGCTGGC GTCGGCGGAA TACCTGATGC GTACGGCGGC CGACGAACAA TCGGTCACCG TGGCCGCACG CGTGAAGAAC AGTTCGCTGC GCATGGCGCG CATGGTCGAT CAGTTGCTCA ATCTCGCCCG CTTGCAGGGC GGCCGCCTGC CGTTGCAGCC GCGCTCGATC GAGTTGGCCA CGCTGTGCCG TTCGGTGATC GACGAATTTG CGTCGCGGGA GAACGGCAAG CAGATCGTGT TCGCGAGCCG CGGCAATACC ACGGGCGCGT GGGACACGGA TCTCGTCTGG CAAGCCGTGT CGAACCTGGT GAGCAATGCG CTGCATCATG GCGCACCGGG CGGCGATATC GGCGTCGAAG TGGATGGCGA AGCGGTGGAT TCAGTGCGCC TGAAGGTCGC CAATCGCGGG ACCATTCCAC CGGAGGTGTT TCCGCATCTG TTCAAGGCCT TTGGGCCCAA TAACAGCGGC GCCCGCTCGC GTGAAGGGCT GGGGCTCGGC CTGCATATCG TGCAGGAGAT CGCGCGCATG CATGGCGGCA GCGTCGGCGT GCATTCCGAG GAAGCCACGG GCACGGTCTT TACGATCGAG CTGCCGAGAA TGGTGACGTT TCAGCGCGGG TCGTTTACTC GGAAG
Details for BuxeA.00863.a.A1.GE31969
HARVESTED ON: 4/5/2011
SEQUENCED ON: 4/11/2011
EXPECTED MW: 42kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MTNSPVNILI VDDIVHNITA LEALLARPDL AVLVADSGTA ALDLLLKHEV ALAILDVNMP GMNGFELAAL MRGSPRTSHV PIIFLTATAQ DASRTFRGYE AGAVDFLYKP FDPRILQSKV DVFVQLEQQK RQLAAQLVTT RQMLEANEML MAVLSHDLRT PLGAVLASAE YLMRTAADEQ SVTVAARVKN SSLRMARMVD QLLNLARLQG GRLPLQPRSI ELATLCRSVI DEFASRENGK QIVFASRGNT TGAWDTDLVW QAVSNLVSNA LHHGAPGGDI GVEVDGEAVD SVRLKVANRG TIPPEVFPHL FKAFGPNNSG ARSREGLGLG LHIVQEIARM HGGSVGVHSE EATGTVFTIE LPRMVTFQRG SFTRK
Validated NT Sequence
ttattacttc cgagtaaacg acccgcgctg aaacgtcacc attctcggca gctcgatcgt aaagaccgtg cccgtggctt cctcggaatg cacgccgacg ctgccgccat gcatgcgcgc gatctcctgc acgatatgca ggccgagccc cagcccttca cgcgagcggg cgccgctgtt attgggccca aaggccttga acagatgcgg aaacacctcc ggtggaatgg tcccgcgatt ggcgaccttc aggcgcactg aatccaccgc ttcgccatcc acttcgacgc cgatatcgcc gcccggtgcg ccatgatgca gcgcattgct caccaggttc gacacggctt gccagacgag atccgtgtcc cacgcgcccg tggtattgcc gcggctcgcg aacacgatct gcttgccgtt ctcccgcgac gcaaattcgt cgatcaccga acggcacagc gtggccaact cgatcgagcg cggctgcaac ggcaggcggc cgccctgcaa gcgggcgaga ttgagcaact gatcgaccat gcgcgccatg cgcagcgaac tgttcttcac gcgtgcggcc acggtgaccg attgttcgtc ggccgccgta cgcatcaggt attccgccga cgccagcacg gcgcccagcg gcgtgcgcag atcatgactc agcaccgcca tcagcatctc gttggcttcc agcatctgcc gcgtggtgac gagctgcgcc gcgagctggc gcttctgctg ttcgagctgc acgaacacat ccaccttgga ttgcaggatg cgcggatcga acggcttgta caggaagtcg accgcgccgg cctcgtagcc gcgaaacgtg cgcgacgcgt cctgcgcggt cgcggtcaga aaaatgatcg gcacgtgcga ggtgcgcggg ctgccacgca tcaacgcggc cagttcgaag ccgttcatgc ccggcatgtt gacgtcgaga atcgccagcg cgacttcgtg cttgagcagc aggtcgagcg ccgcggtgcc cgaatcggcc accagcacgg caagatccgg ccgcgccagc aaggcctcga gcgccgtgat gttgtggacg atgtcgtcga cgatcagaat gttcacgggt gaattcgtca tcgaaccagg accctgggtc tgagcttcca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNSPVNIL IVDDIVHNIT ALEALLARPD LAVLVADSGT AALDLLLKHE VALAILDVNM PGMNGFELAA LMRGSPRTSH VPIIFLTATA QDASRTFRGY EAGAVDFLYK PFDPRILQSK VDVFVQLEQQ KRQLAAQLVT TRQMLEANEM LMAVLSHDLR TPLGAVLASA EYLMRTAADE QSVTVAARVK NSSLRMARMV DQLLNLARLQ GGRLPLQPRS IELATLCRSV IDEFASRENG KQIVFASRGN TTGAWDTDLV WQAVSNLVSN ALHHGAPGGD IGVEVDGEAV DSVRLKVANR GTIPPEVFPH LFKAFGPNNS GARSREGLGL GLHIVQEIAR MHGGSVGVHS EEATGTVFTI ELPRMVTFQR GSFTRK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgaattca cccgtgaaca ttctgatcgt cgacgacatc gtccacaaca tcacggcgct cgaggccttg ctggcgcggc cggatcttgc cgtgctggtg gccgattcgg gcaccgcggc gctcgacctg ctgctcaagc acgaagtcgc gctggcgatt ctcgacgtca acatgccggg catgaacggc ttcgaactgg ccgcgttgat gcgtggcagc ccgcgcacct cgcacgtgcc gatcattttt ctgaccgcga ccgcgcagga cgcgtcgcgc acgtttcgcg gctacgaggc cggcgcggtc gacttcctgt acaagccgtt cgatccgcgc atcctgcaat ccaaggtgga tgtgttcgtg cagctcgaac agcagaagcg ccagctcgcg gcgcagctcg tcaccacgcg gcagatgctg gaagccaacg agatgctgat ggcggtgctg agtcatgatc tgcgcacgcc gctgggcgcc gtgctggcgt cggcggaata cctgatgcgt acggcggccg acgaacaatc ggtcaccgtg gccgcacgcg tgaagaacag ttcgctgcgc atggcgcgca tggtcgatca gttgctcaat ctcgcccgct tgcagggcgg ccgcctgccg ttgcagccgc gctcgatcga gttggccacg ctgtgccgtt cggtgatcga cgaatttgcg tcgcgggaga acggcaagca gatcgtgttc gcgagccgcg gcaataccac gggcgcgtgg gacacggatc tcgtctggca agccgtgtcg aacctggtga gcaatgcgct gcatcatggc gcaccgggcg gcgatatcgg cgtcgaagtg gatggcgaag cggtggattc agtgcgcctg aaggtcgcca atcgcgggac cattccaccg gaggtgtttc cgcatctgtt caaggccttt gggcccaata acagcggcgc ccgctcgcgt gaagggctgg ggctcggcct gcatatcgtg caggagatcg cgcgcatgca tggcggcagc gtcggcgtgc attccgagga agccacgggc acggtcttta cgatcgagct gccgagaatg gtgacgtttc agcgcgggtc gtttactcgg aagtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BuxeA.00863.a.A1.PS01108
PURIFICATION DATe: 6/9/2011
CONCENTRATION: 24.74mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MTNSPVNILI VDDIVHNITA LEALLARPDL AVLVADSGTA ALDLLLKHEV ALAILDVNMP GMNGFELAAL MRGSPRTSHV PIIFLTATAQ DASRTFRGYE AGAVDFLYKP FDPRILQSKV DVFVQLEQQK RQLAAQLVTT RQMLEANEML MAVLSHDLRT PLGAVLASAE YLMRTAADEQ SVTVAARVKN SSLRMARMVD QLLNLARLQG GRLPLQPRSI ELATLCRSVI DEFASRENGK QIVFASRGNT TGAWDTDLVW QAVSNLVSNA LHHGAPGGDI GVEVDGEAVD SVRLKVANRG TIPPEVFPHL FKAFGPNNSG ARSREGLGLG LHIVQEIARM HGGSVGVHSE EATGTVFTIE LPRMVTFQRG SFTRK
Validated NT Sequence
ttattacttc cgagtaaacg acccgcgctg aaacgtcacc attctcggca gctcgatcgt aaagaccgtg cccgtggctt cctcggaatg cacgccgacg ctgccgccat gcatgcgcgc gatctcctgc acgatatgca ggccgagccc cagcccttca cgcgagcggg cgccgctgtt attgggccca aaggccttga acagatgcgg aaacacctcc ggtggaatgg tcccgcgatt ggcgaccttc aggcgcactg aatccaccgc ttcgccatcc acttcgacgc cgatatcgcc gcccggtgcg ccatgatgca gcgcattgct caccaggttc gacacggctt gccagacgag atccgtgtcc cacgcgcccg tggtattgcc gcggctcgcg aacacgatct gcttgccgtt ctcccgcgac gcaaattcgt cgatcaccga acggcacagc gtggccaact cgatcgagcg cggctgcaac ggcaggcggc cgccctgcaa gcgggcgaga ttgagcaact gatcgaccat gcgcgccatg cgcagcgaac tgttcttcac gcgtgcggcc acggtgaccg attgttcgtc ggccgccgta cgcatcaggt attccgccga cgccagcacg gcgcccagcg gcgtgcgcag atcatgactc agcaccgcca tcagcatctc gttggcttcc agcatctgcc gcgtggtgac gagctgcgcc gcgagctggc gcttctgctg ttcgagctgc acgaacacat ccaccttgga ttgcaggatg cgcggatcga acggcttgta caggaagtcg accgcgccgg cctcgtagcc gcgaaacgtg cgcgacgcgt cctgcgcggt cgcggtcaga aaaatgatcg gcacgtgcga ggtgcgcggg ctgccacgca tcaacgcggc cagttcgaag ccgttcatgc ccggcatgtt gacgtcgaga atcgccagcg cgacttcgtg cttgagcagc aggtcgagcg ccgcggtgcc cgaatcggcc accagcacgg caagatccgg ccgcgccagc aaggcctcga gcgccgtgat gttgtggacg atgtcgtcga cgatcagaat gttcacgggt gaattcgtca tcgaaccagg accctgggtc tgagcttcca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNSPVNIL IVDDIVHNIT ALEALLARPD LAVLVADSGT AALDLLLKHE VALAILDVNM PGMNGFELAA LMRGSPRTSH VPIIFLTATA QDASRTFRGY EAGAVDFLYK PFDPRILQSK VDVFVQLEQQ KRQLAAQLVT TRQMLEANEM LMAVLSHDLR TPLGAVLASA EYLMRTAADE QSVTVAARVK NSSLRMARMV DQLLNLARLQ GGRLPLQPRS IELATLCRSV IDEFASRENG KQIVFASRGN TTGAWDTDLV WQAVSNLVSN ALHHGAPGGD IGVEVDGEAV DSVRLKVANR GTIPPEVFPH LFKAFGPNNS GARSREGLGL GLHIVQEIAR MHGGSVGVHS EEATGTVFTI ELPRMVTFQR GSFTRK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGAATTCA CCCGTGAACA TTCTGATCGT CGACGACATC GTCCACAACA TCACGGCGCT CGAGGCCTTG CTGGCGCGGC CGGATCTTGC CGTGCTGGTG GCCGATTCGG GCACCGCGGC GCTCGACCTG CTGCTCAAGC ACGAAGTCGC GCTGGCGATT CTCGACGTCA ACATGCCGGG CATGAACGGC TTCGAACTGG CCGCGTTGAT GCGTGGCAGC CCGCGCACCT CGCACGTGCC GATCATTTTT CTGACCGCGA CCGCGCAGGA CGCGTCGCGC ACGTTTCGCG GCTACGAGGC CGGCGCGGTC GACTTCCTGT ACAAGCCGTT CGATCCGCGC ATCCTGCAAT CCAAGGTGGA TGTGTTCGTG CAGCTCGAAC AGCAGAAGCG CCAGCTCGCG GCGCAGCTCG TCACCACGCG GCAGATGCTG GAAGCCAACG AGATGCTGAT GGCGGTGCTG AGTCATGATC TGCGCACGCC GCTGGGCGCC GTGCTGGCGT CGGCGGAATA CCTGATGCGT ACGGCGGCCG ACGAACAATC GGTCACCGTG GCCGCACGCG TGAAGAACAG TTCGCTGCGC ATGGCGCGCA TGGTCGATCA GTTGCTCAAT CTCGCCCGCT TGCAGGGCGG CCGCCTGCCG TTGCAGCCGC GCTCGATCGA GTTGGCCACG CTGTGCCGTT CGGTGATCGA CGAATTTGCG TCGCGGGAGA ACGGCAAGCA GATCGTGTTC GCGAGCCGCG GCAATACCAC GGGCGCGTGG GACACGGATC TCGTCTGGCA AGCCGTGTCG AACCTGGTGA GCAATGCGCT GCATCATGGC GCACCGGGCG GCGATATCGG CGTCGAAGTG GATGGCGAAG CGGTGGATTC AGTGCGCCTG AAGGTCGCCA ATCGCGGGAC CATTCCACCG GAGGTGTTTC CGCATCTGTT CAAGGCCTTT GGGCCCAATA ACAGCGGCGC CCGCTCGCGT GAAGGGCTGG GGCTCGGCCT GCATATCGTG CAGGAGATCG CGCGCATGCA TGGCGGCAGC GTCGGCGTGC ATTCCGAGGA AGCCACGGGC ACGGTCTTTA CGATCGAGCT GCCGAGAATG GTGACGTTTC AGCGCGGGTC GTTTACTCGG AAGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA