BuxeA.00171.c
3-oxoacyl-[acyl-carrier-protein] synthase III
| CENTER ID: | BuxeA.00171.c |
| ORGANISM: | Burkholderia xenovorans LB400 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00171.c.A1.GE33035 | Full length( BuxeA.00171.c ) | 1 | 329 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuxeA.00171.c.A1.PW33645 | Full length( BuxeA.00171.c ) | 1 | 329 |
Structures
4DFE
DEPOSITED: 1/23/2012
DETERMINATION: XRay
CLONE: BuxeA.00171.c.A1.GE33035
PROTEIN: BuxeA.00171.c.A1.PW33645
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129509 |
| PATRIC ID: | fig|266265.5.peg.3503 |
| RefSeq: | YP_559926.1 |
| UniProt: | Q13VL5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQSTIYSRV LGTGSYLPPN RVTNQDLAKR LAEQGIETSD EWIVARTGIH ARYFAEPDVT TSDLAFIASQ RAIEAADIDP QSIDLIIVAT STPDFVFPST ACLLQNKLGI RNHGAAFDVQ AVCSGFAYAV ATADSFIRSG QHRTALVIGA ETFSRILDFK DRTTCVLFGD GAGAVILQAS DEPGVLASAL HADGSHSNIL CTPGNVNGGV VSGSAFLHMD GQAVFKLAVN VLEKVAVEAL EKANLSAEQI DWLIPHQANI RIMQSTCRKL GLPQERMIVT VGEHGNTSAA SIPLALDVAV RDGRIKRGQN VLIEGVGGGF TWGASVIRY
NT Sequence
ATGGCTCAAT CGACAATTTA TTCCCGCGTG CTGGGCACGG GCAGTTATCT GCCGCCCAAC CGCGTCACCA ATCAGGATCT GGCGAAACGT CTCGCCGAGC AGGGTATCGA GACAAGCGAC GAGTGGATCG TCGCCCGTAC CGGCATCCAC GCGCGCTACT TCGCGGAACC GGATGTCACG ACCAGCGATC TGGCGTTCAT CGCTTCGCAG CGCGCGATCG AAGCCGCGGA TATCGACCCG CAATCCATCG ACCTGATCAT CGTTGCAACC TCCACGCCCG ACTTCGTGTT TCCGAGCACG GCGTGCCTGT TGCAGAACAA GCTTGGCATC AGGAACCACG GCGCGGCGTT CGACGTGCAG GCCGTCTGTT CGGGTTTTGC CTATGCGGTG GCGACGGCGG ACAGCTTCAT TCGCAGCGGT CAGCATCGTA CGGCGCTCGT GATCGGCGCG GAAACCTTCT CGCGCATTCT CGACTTCAAG GACCGGACCA CCTGCGTGCT GTTCGGCGAC GGCGCGGGCG CGGTGATCCT GCAGGCGTCC GACGAACCGG GCGTGCTTGC CAGCGCGTTG CACGCGGACG GCAGCCATTC GAACATTCTT TGCACGCCGG GCAACGTGAA CGGCGGTGTG GTATCCGGCA GCGCGTTCCT GCATATGGAC GGGCAGGCCG TGTTCAAGCT CGCCGTCAAC GTGCTCGAAA AAGTCGCGGT CGAAGCGCTC GAGAAAGCCA ATCTGTCGGC TGAGCAGATC GACTGGCTGA TTCCGCATCA GGCGAATATC CGCATCATGC AAAGCACCTG CCGCAAGCTC GGTTTGCCGC AGGAACGCAT GATCGTCACA GTGGGCGAGC ACGGCAACAC GTCGGCTGCG TCCATTCCGC TCGCACTCGA CGTCGCGGTG CGCGACGGCC GCATCAAGCG CGGCCAGAAC GTGCTGATCG AAGGCGTCGG CGGCGGCTTC ACGTGGGGCG CGTCGGTTAT CCGCTAC
Details for BuxeA.00171.c.A1.GE33035
| HARVESTED ON: | 8/16/2011 |
| SEQUENCED ON: | 8/22/2011 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MAQSTIYSRV LGTGSYLPPN RVTNQDLAKR LAEQGIETSD EWIVARTGIH ARYFAEPDVT TSDLAFIASQ RAIEAADIDP QSIDLIIVAT STPDFVFPST ACLLQNKLGI RNHGAAFDVQ AVCSGFAYAV ATADSFIRSG QHRTALVIGA ETFSRILDFK DRTTCVLFGD GAGAVILQAS DEPGVLASAL HADGSHSNIL CTPGNVNGGV VSGSAFLHMD GQAVFKLAVN VLEKVAVEAL EKANLSAEQI DWLIPHQANI RIMQSTCRKL GLPQERMIVT VGEHGNTSAA SIPLALDVAV RDGRIKRGQN VLIEGVGGGF TWGASVIRY
Validated NT Sequence
ttattagtag cggataaccg acgcgcccca cgtgaagccg ccgccgacgc cttcgatcag cacgttctgg ccgcgcttga tgcggccgtc gcgcaccgcg acgtcgagtg cgagcggaat ggacgcagcc gacgtgttgc cgtgctcgcc cactgtgacg atcatgcgtt cctgcggcaa accgagcttg cggcaggtgc tttgcatgat gcggatattc gcctgatgcg gaatcagcca gtcgatctgc tcagccgaca gattggcttt ctcgagcgct tcgaccgcga ctttttcgag cacgttgacg gcgagcttga acacggcctg cccgtccata tgcaggaacg cgctgccgga taccacaccg ccgttcacgt tgcccggcgt gcaaagaatg ttcgaatggc tgccgtccgc gtgcaacgcg ctggcaagca cgcccggttc gtcggacgcc tgcaggatca ccgcgcccgc gccgtcgccg aacagcacgc aggtggtccg gtccttgaag tcgagaatgc gcgagaaggt ttccgcgccg atcacgagcg ccgtacgatg ctgaccgctg cgaatgaagc tgtccgccgt cgccaccgca taggcaaaac ccgaacagac ggcctgcacg tcgaacgccg cgccgtggtt cctgatgcca agcttgttct gcaacaggca cgccgtgctc ggaaacacga agtcgggcgt ggaggttgca acgatgatca ggtcgatgga ttgcgggtcg atatccgcgg cttcgatcgc gcgctgcgaa gcgatgaacg ccagatcgct ggtcgtgaca tccggttccg cgaagtagcg cgcgtggatg ccggtacggg cgacgatcca ctcgtcgctt gtctcgatac cctgctcggc gagacgtttc gccagatcct gattggtgac gcggttgggc ggcagataac tgcccgtgcc cagcacgcgg gaataaattg tcgattgagc catcgaacca ggaccctggg tctgagcttc cagggtac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQSTIYSR VLGTGSYLPP NRVTNQDLAK RLAEQGIETS DEWIVARTGI HARYFAEPDV TTSDLAFIAS QRAIEAADID PQSIDLIIVA TSTPDFVFPS TACLLQNKLG IRNHGAAFDV QAVCSGFAYA VATADSFIRS GQHRTALVIG AETFSRILDF KDRTTCVLFG DGAGAVILQA SDEPGVLASA LHADGSHSNI LCTPGNVNGG VVSGSAFLHM DGQAVFKLAV NVLEKVAVEA LEKANLSAEQ IDWLIPHQAN IRIMQSTCRK LGLPQERMIV TVGEHGNTSA ASIPLALDVA VRDGRIKRGQ NVLIEGVGGG FTWGASVIRY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctcaatcg acaatttatt cccgcgtgct gggcacgggc agttatctgc cgcccaaccg cgtcaccaat caggatctgg cgaaacgtct cgccgagcag ggtatcgaga caagcgacga gtggatcgtc gcccgtaccg gcatccacgc gcgctacttc gcggaaccgg atgtcacgac cagcgatctg gcgttcatcg cttcgcagcg cgcgatcgaa gccgcggata tcgacccgca atccatcgac ctgatcatcg ttgcaacctc cacgcccgac ttcgtgtttc cgagcacggc gtgcctgttg cagaacaagc ttggcatcag gaaccacggc gcggcgttcg acgtgcaggc cgtctgttcg ggttttgcct atgcggtggc gacggcggac agcttcattc gcagcggtca gcatcgtacg gcgctcgtga tcggcgcgga aaccttctcg cgcattctcg acttcaagga ccggaccacc tgcgtgctgt tcggcgacgg cgcgggcgcg gtgatcctgc aggcgtccga cgaaccgggc gtgcttgcca gcgcgttgca cgcggacggc agccattcga acattctttg cacgccgggc aacgtgaacg gcggtgtggt atccggcagc gcgttcctgc atatggacgg gcaggccgtg ttcaagctcg ccgtcaacgt gctcgaaaaa gtcgcggtcg aagcgctcga gaaagccaat ctgtcggctg agcagatcga ctggctgatt ccgcatcagg cgaatatccg catcatgcaa agcacctgcc gcaagctcgg tttgccgcag gaacgcatga tcgtcacagt gggcgagcac ggcaacacgt cggctgcgtc cattccgctc gcactcgacg tcgcggtgcg cgacggccgc atcaagcgcg gccagaacgt gctgatcgaa ggcgtcggcg gcggcttcac gtggggcgcg tcggttatcc gctactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BuxeA.00171.c.A1.PW33645
| PURIFICATION DATe: | 11/11/2011 |
| CONCENTRATION: | 34.86mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAQSTIYSRV LGTGSYLPPN RVTNQDLAKR LAEQGIETSD EWIVARTGIH ARYFAEPDVT TSDLAFIASQ RAIEAADIDP QSIDLIIVAT STPDFVFPST ACLLQNKLGI RNHGAAFDVQ AVCSGFAYAV ATADSFIRSG QHRTALVIGA ETFSRILDFK DRTTCVLFGD GAGAVILQAS DEPGVLASAL HADGSHSNIL CTPGNVNGGV VSGSAFLHMD GQAVFKLAVN VLEKVAVEAL EKANLSAEQI DWLIPHQANI RIMQSTCRKL GLPQERMIVT VGEHGNTSAA SIPLALDVAV RDGRIKRGQN VLIEGVGGGF TWGASVIRY
Validated NT Sequence
ttattagtag cggataaccg acgcgcccca cgtgaagccg ccgccgacgc cttcgatcag cacgttctgg ccgcgcttga tgcggccgtc gcgcaccgcg acgtcgagtg cgagcggaat ggacgcagcc gacgtgttgc cgtgctcgcc cactgtgacg atcatgcgtt cctgcggcaa accgagcttg cggcaggtgc tttgcatgat gcggatattc gcctgatgcg gaatcagcca gtcgatctgc tcagccgaca gattggcttt ctcgagcgct tcgaccgcga ctttttcgag cacgttgacg gcgagcttga acacggcctg cccgtccata tgcaggaacg cgctgccgga taccacaccg ccgttcacgt tgcccggcgt gcaaagaatg ttcgaatggc tgccgtccgc gtgcaacgcg ctggcaagca cgcccggttc gtcggacgcc tgcaggatca ccgcgcccgc gccgtcgccg aacagcacgc aggtggtccg gtccttgaag tcgagaatgc gcgagaaggt ttccgcgccg atcacgagcg ccgtacgatg ctgaccgctg cgaatgaagc tgtccgccgt cgccaccgca taggcaaaac ccgaacagac ggcctgcacg tcgaacgccg cgccgtggtt cctgatgcca agcttgttct gcaacaggca cgccgtgctc ggaaacacga agtcgggcgt ggaggttgca acgatgatca ggtcgatgga ttgcgggtcg atatccgcgg cttcgatcgc gcgctgcgaa gcgatgaacg ccagatcgct ggtcgtgaca tccggttccg cgaagtagcg cgcgtggatg ccggtacggg cgacgatcca ctcgtcgctt gtctcgatac cctgctcggc gagacgtttc gccagatcct gattggtgac gcggttgggc ggcagataac tgcccgtgcc cagcacgcgg gaataaattg tcgattgagc catcgaacca ggaccctggg tctgagcttc cagggtac
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQSTIYSR VLGTGSYLPP NRVTNQDLAK RLAEQGIETS DEWIVARTGI HARYFAEPDV TTSDLAFIAS QRAIEAADID PQSIDLIIVA TSTPDFVFPS TACLLQNKLG IRNHGAAFDV QAVCSGFAYA VATADSFIRS GQHRTALVIG AETFSRILDF KDRTTCVLFG DGAGAVILQA SDEPGVLASA LHADGSHSNI LCTPGNVNGG VVSGSAFLHM DGQAVFKLAV NVLEKVAVEA LEKANLSAEQ IDWLIPHQAN IRIMQSTCRK LGLPQERMIV TVGEHGNTSA ASIPLALDVA VRDGRIKRGQ NVLIEGVGGG FTWGASVIRY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTCAATCG ACAATTTATT CCCGCGTGCT GGGCACGGGC AGTTATCTGC CGCCCAACCG CGTCACCAAT CAGGATCTGG CGAAACGTCT CGCCGAGCAG GGTATCGAGA CAAGCGACGA GTGGATCGTC GCCCGTACCG GCATCCACGC GCGCTACTTC GCGGAACCGG ATGTCACGAC CAGCGATCTG GCGTTCATCG CTTCGCAGCG CGCGATCGAA GCCGCGGATA TCGACCCGCA ATCCATCGAC CTGATCATCG TTGCAACCTC CACGCCCGAC TTCGTGTTTC CGAGCACGGC GTGCCTGTTG CAGAACAAGC TTGGCATCAG GAACCACGGC GCGGCGTTCG ACGTGCAGGC CGTCTGTTCG GGTTTTGCCT ATGCGGTGGC GACGGCGGAC AGCTTCATTC GCAGCGGTCA GCATCGTACG GCGCTCGTGA TCGGCGCGGA AACCTTCTCG CGCATTCTCG ACTTCAAGGA CCGGACCACC TGCGTGCTGT TCGGCGACGG CGCGGGCGCG GTGATCCTGC AGGCGTCCGA CGAACCGGGC GTGCTTGCCA GCGCGTTGCA CGCGGACGGC AGCCATTCGA ACATTCTTTG CACGCCGGGC AACGTGAACG GCGGTGTGGT ATCCGGCAGC GCGTTCCTGC ATATGGACGG GCAGGCCGTG TTCAAGCTCG CCGTCAACGT GCTCGAAAAA GTCGCGGTCG AAGCGCTCGA GAAAGCCAAT CTGTCGGCTG AGCAGATCGA CTGGCTGATT CCGCATCAGG CGAATATCCG CATCATGCAA AGCACCTGCC GCAAGCTCGG TTTGCCGCAG GAACGCATGA TCGTCACAGT GGGCGAGCAC GGCAACACGT CGGCTGCGTC CATTCCGCTC GCACTCGACG TCGCGGTGCG CGACGGCCGC ATCAAGCGCG GCCAGAACGT GCTGATCGAA GGCGTCGGCG GCGGCTTCAC GTGGGGCGCG TCGGTTATCC GCTACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA