BuxeA.00141.a

Putative acyl transferase

CENTER ID: BuxeA.00141.a
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00141.a.B1.GE39441 Full length( BuxeA.00141.a ) 1 310
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00141.a.B1.PS37852 Full length( BuxeA.00141.a ) 1 310

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.5890
RefSeq: YP_553468.1
UniProt: Q13P71

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLALLFPGQG AQTDGFLHRL PRHRAVRDTL EEASQVLGAD VLTLDTPDAL RSTVAVQIGL TVAGVAITRA LADEQLVAEI SAGLSVGAYA AAVGCGAIHF ADALKMVRRR AELMETAYPS GYGLAAISGL TEYQVETLAA RHADENRQHV YIGNVNAPRQ IVVAGANDAL DAFAARALAA GARKAARLAV SVPSHCELLA QATDGLVAYA KEIPFHAPHS TYVGNRGGRP LYTADAIRDD LATNMRYTVR WFDALAVMQE MGARVLIEAP PGQVLTDIAR EYFPDSTALA ASTFPFDRLV ATARRRLDAG
NT Sequence
ATGCTCGCCC TGCTTTTTCC GGGACAAGGC GCGCAGACCG ATGGGTTTCT GCATCGTCTG CCGCGGCACC GTGCCGTGCG CGACACGCTG GAGGAAGCTT CGCAGGTGCT CGGCGCCGAT GTGCTCACGC TGGACACGCC GGACGCTTTG CGCTCGACCG TCGCTGTGCA GATCGGCCTG ACGGTCGCAG GCGTGGCGAT CACGCGCGCA CTGGCTGACG AACAACTCGT GGCGGAGATC AGCGCGGGTT TGTCCGTCGG CGCGTACGCC GCTGCAGTCG GTTGCGGTGC GATTCATTTC GCAGACGCGT TGAAGATGGT TCGCCGCCGC GCCGAGTTGA TGGAGACCGC ATACCCGTCC GGCTACGGCC TCGCGGCAAT CTCGGGTTTG ACCGAATATC AGGTGGAAAC GCTCGCCGCG CGGCATGCGG ATGAAAACCG GCAGCATGTC TATATCGGCA ACGTGAACGC GCCGCGCCAG ATCGTCGTGG CGGGCGCAAA CGACGCGCTC GACGCGTTCG CCGCACGCGC GCTGGCCGCA GGCGCGCGCA AGGCCGCGCG CCTCGCGGTG AGCGTCCCCT CGCATTGCGA ATTGCTCGCG CAGGCCACGG ACGGGTTGGT GGCGTACGCA AAGGAAATAC CGTTTCATGC GCCGCACAGC ACGTATGTCG GCAATCGCGG CGGCCGCCCT CTATACACGG CGGACGCGAT CCGCGACGAT CTCGCAACCA ATATGCGCTA CACCGTACGC TGGTTCGACG CGCTCGCGGT CATGCAGGAA ATGGGCGCGC GCGTACTGAT CGAAGCGCCG CCGGGCCAGG TCCTGACCGA CATCGCGCGC GAATACTTTC CGGACAGCAC GGCGCTCGCC GCCAGCACCT TTCCGTTCGA TCGACTCGTC GCCACCGCGC GGCGGCGTCT GGATGCCGGC
Details for BuxeA.00141.a.B1.GE39441
HARVESTED ON: 8/5/2015
SEQUENCED ON: 8/13/2015
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML ALLFPGQGAQ TDGFLHRLPR HRAVRDTLEE ASQVLGADVL TLDTPDALRS TVAVQIGLTV AGVAITRALA DEQLVAEISA GLSVGAYAAA VGCGAIHFAD ALKMVRRRAE LMETAYPSGY GLAAISGLTE YQVETLAARH ADENRQHVYI GNVNAPRQIV VAGANDALDA FAARALAAGA RKAARLAVSV PSHCELLAQA TDGLVAYAKE IPFHAPHSTY VGNRGGRPLY TADAIRDDLA TNMRYTVRWF DALAVMQEMG ARVLIEAPPG QVLTDIAREY FPDSTALAAS TFPFDRLVAT XXRRLDAG
Validated NT Sequence
ttactcactt agccggcatc cagacgccgn nnnnnngtgg cgacgagtcg atcgaacgga aaggtgctgg cggcgagcgc cgtgctgtcc ggaaagtatt cgcgcgcgat gtcggtcagg acctggcccg gcggcgcttc gatcagtacg cgcgcgccca tttcctgcat gaccgcgagc gcgtcgaacc agcgtacggt gtagcgcata ttggttgcga gatcgtcgcg gatcgcgtcc gccgtgtata gagggcggcc gccgcgattg ccgacatacg tgctgtgcgg cgcatgaaac ggtatttcct ttgcgtacgc caccaacccg tccgtggcct gcgcgagcaa ttcgcaatgc gaggggacgc tcaccgcgag gcgcgcggcc ttgcgcgcgc ctgcggccag cgcgcgtgcg gcgaacgcgt cgagcgcgtc gtttgcgccc gccacgacga tctggcgcgg cgcgttcacg ttgccgatat agacatgctg ccggttttca tccgcatgcc gcgcggcgag cgtttccacc tgatattcgg tcaaacccga gattgccgcg aggccgtagc cggacgggta tgcggtctcc atcaactcgg cgcggcggcg aaccatcttc aacgcgtctg cgaaatgaat cgcaccgcaa ccgactgcag cggcgtacgc gccgacggac aaacccgcgc tgatctccgc cacgagttgt tcgtcagcca gtgcgcgcgt gatcgccacg cctgcgaccg tcaggccgat ctgcacagcg acggtcgagc gcaaagcgtc cggcgtgtcc agcgtgagca catcggcgcc gagcacctgc gaagcttcct ccagcgtgtc gcgcacggca cggtgccgcg gcagacgatg cagaaaccca tcggtctgcg cgccttgtcc cggaaaaagc agggcgagca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHML ALLFPGQGAQ TDGFLHRLPR HRAVRDTLEE ASQVLGADVL TLDTPDALRS TVAVQIGLTV AGVAITRALA DEQLVAEISA GLSVGAYAAA VGCGAIHFAD ALKMVRRRAE LMETAYPSGY GLAAISGLTE YQVETLAARH ADENRQHVYI GNVNAPRQIV VAGANDALDA FAARALAAGA RKAARLAVSV PSHCELLAQA TDGLVAYAKE IPFHAPHSTY VGNRGGRPLY TADAIRDDLA TNMRYTVRWF DALAVMQEMG ARVLIEAPPG QVLTDIAREY FPDSTALAAS TFPFDRLVAT ARRRLDAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctcg ccctgctttt tccgggacaa ggcgcgcaga ccgatgggtt tctgcatcgt ctgccgcggc accgtgccgt gcgcgacacg ctggaggaag cttcgcaggt gctcggcgcc gatgtgctca cgctggacac gccggacgct ttgcgctcga ccgtcgctgt gcagatcggc ctgacggtcg caggcgtggc gatcacgcgc gcactggctg acgaacaact cgtggcggag atcagcgcgg gtttgtccgt cggcgcgtac gccgctgcag tcggttgcgg tgcgattcat ttcgcagacg cgttgaagat ggttcgccgc cgcgccgagt tgatggagac cgcatacccg tccggctacg gcctcgcggc aatctcgggt ttgaccgaat atcaggtgga aacgctcgcc gcgcggcatg cggatgaaaa ccggcagcat gtctatatcg gcaacgtgaa cgcgccgcgc cagatcgtcg tggcgggcgc aaacgacgcg ctcgacgcgt tcgccgcacg cgcgctggcc gcaggcgcgc gcaaggccgc gcgcctcgcg gtgagcgtcc cctcgcattg cgaattgctc gcgcaggcca cggacgggtt ggtggcgtac gcaaaggaaa taccgtttca tgcgccgcac agcacgtatg tcggcaatcg cggcggccgc cctctataca cggcggacgc gatccgcgac gatctcgcaa ccaatatgcg ctacaccgta cgctggttcg acgcgctcgc ggtcatgcag gaaatgggcg cgcgcgtact gatcgaagcg ccgccgggcc aggtcctgac cgacatcgcg cgcgaatact ttccggacag cacggcgctc gccgccagca cctttccgtt cgatcgactc gtcgccaccg cgcggcggcg tctggatgcc ggctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BuxeA.00141.a.B1.PS37852
PURIFICATION DATe: 2/25/2016
CONCENTRATION: 19mg/ml
OBSERVED MW: 32kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML ALLFPGQGAQ TDGFLHRLPR HRAVRDTLEE ASQVLGADVL TLDTPDALRS TVAVQIGLTV AGVAITRALA DEQLVAEISA GLSVGAYAAA VGCGAIHFAD ALKMVRRRAE LMETAYPSGY GLAAISGLTE YQVETLAARH ADENRQHVYI GNVNAPRQIV VAGANDALDA FAARALAAGA RKAARLAVSV PSHCELLAQA TDGLVAYAKE IPFHAPHSTY VGNRGGRPLY TADAIRDDLA TNMRYTVRWF DALAVMQEMG ARVLIEAPPG QVLTDIAREY FPDSTALAAS TFPFDRLVAT XXRRLDAG
Validated NT Sequence
ttactcactt agccggcatc cagacgccgn nnnnnngtgg cgacgagtcg atcgaacgga aaggtgctgg cggcgagcgc cgtgctgtcc ggaaagtatt cgcgcgcgat gtcggtcagg acctggcccg gcggcgcttc gatcagtacg cgcgcgccca tttcctgcat gaccgcgagc gcgtcgaacc agcgtacggt gtagcgcata ttggttgcga gatcgtcgcg gatcgcgtcc gccgtgtata gagggcggcc gccgcgattg ccgacatacg tgctgtgcgg cgcatgaaac ggtatttcct ttgcgtacgc caccaacccg tccgtggcct gcgcgagcaa ttcgcaatgc gaggggacgc tcaccgcgag gcgcgcggcc ttgcgcgcgc ctgcggccag cgcgcgtgcg gcgaacgcgt cgagcgcgtc gtttgcgccc gccacgacga tctggcgcgg cgcgttcacg ttgccgatat agacatgctg ccggttttca tccgcatgcc gcgcggcgag cgtttccacc tgatattcgg tcaaacccga gattgccgcg aggccgtagc cggacgggta tgcggtctcc atcaactcgg cgcggcggcg aaccatcttc aacgcgtctg cgaaatgaat cgcaccgcaa ccgactgcag cggcgtacgc gccgacggac aaacccgcgc tgatctccgc cacgagttgt tcgtcagcca gtgcgcgcgt gatcgccacg cctgcgaccg tcaggccgat ctgcacagcg acggtcgagc gcaaagcgtc cggcgtgtcc agcgtgagca catcggcgcc gagcacctgc gaagcttcct ccagcgtgtc gcgcacggca cggtgccgcg gcagacgatg cagaaaccca tcggtctgcg cgccttgtcc cggaaaaagc agggcgagca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHML ALLFPGQGAQ TDGFLHRLPR HRAVRDTLEE ASQVLGADVL TLDTPDALRS TVAVQIGLTV AGVAITRALA DEQLVAEISA GLSVGAYAAA VGCGAIHFAD ALKMVRRRAE LMETAYPSGY GLAAISGLTE YQVETLAARH ADENRQHVYI GNVNAPRQIV VAGANDALDA FAARALAAGA RKAARLAVSV PSHCELLAQA TDGLVAYAKE IPFHAPHSTY VGNRGGRPLY TADAIRDDLA TNMRYTVRWF DALAVMQEMG ARVLIEAPPG QVLTDIAREY FPDSTALAAS TFPFDRLVAT ARRRLDAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTCG CCCTGCTTTT TCCGGGACAA GGCGCGCAGA CCGATGGGTT TCTGCATCGT CTGCCGCGGC ACCGTGCCGT GCGCGACACG CTGGAGGAAG CTTCGCAGGT GCTCGGCGCC GATGTGCTCA CGCTGGACAC GCCGGACGCT TTGCGCTCGA CCGTCGCTGT GCAGATCGGC CTGACGGTCG CAGGCGTGGC GATCACGCGC GCACTGGCTG ACGAACAACT CGTGGCGGAG ATCAGCGCGG GTTTGTCCGT CGGCGCGTAC GCCGCTGCAG TCGGTTGCGG TGCGATTCAT TTCGCAGACG CGTTGAAGAT GGTTCGCCGC CGCGCCGAGT TGATGGAGAC CGCATACCCG TCCGGCTACG GCCTCGCGGC AATCTCGGGT TTGACCGAAT ATCAGGTGGA AACGCTCGCC GCGCGGCATG CGGATGAAAA CCGGCAGCAT GTCTATATCG GCAACGTGAA CGCGCCGCGC CAGATCGTCG TGGCGGGCGC AAACGACGCG CTCGACGCGT TCGCCGCACG CGCGCTGGCC GCAGGCGCGC GCAAGGCCGC GCGCCTCGCG GTGAGCGTCC CCTCGCATTG CGAATTGCTC GCGCAGGCCA CGGACGGGTT GGTGGCGTAC GCAAAGGAAA TACCGTTTCA TGCGCCGCAC AGCACGTATG TCGGCAATCG CGGCGGCCGC CCTCTATACA CGGCGGACGC GATCCGCGAC GATCTCGCAA CCAATATGCG CTACACCGTA CGCTGGTTCG ACGCGCTCGC GGTCATGCAG GAAATGGGCG CGCGCGTACT GATCGAAGCG CCGCCGGGCC AGGTCCTGAC CGACATCGCG CGCGAATACT TTCCGGACAG CACGGCGCTC GCCGCCAGCA CCTTTCCGTT CGATCGACTC GTCGCCACCG CGCGGCGGCG TCTGGATGCC GGCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT