BuxeA.00137.b

UDP-N-acetylmuramate/L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase, MurC

CENTER ID: BuxeA.00137.b
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00137.b.A2.GE33032 Mature protein( BuxeA.00137.b ) 32 464
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.4079
RefSeq: YP_560449.1
UniProt: Q13U42

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHIHILGICG TFMGGLAVLA RGAGHTVTGC DAGVYPPMST QLEAQGIGLI EGYDADQLNG LNADLFVIGN VVSRGNPLME AILDRGLPYT SGPQWLGEHV LNGKWVLAVA GTHGKTTTSS MLTWLLEDAG LNPGFLIGGV PLNFGVSARL TDSSFFVIEA DEYDTAFFDK RSKFVHYRPK TAVLNNLEFD HADIFPDLAA IETQFHHLIR TVPGIGRIVT NGREDALERV LTRGCWSEVE RFGVQGGWET LPAEDGVPVD ERFAVYHNSE RVGAVEWQVQ GEHNRMNALA AIAAARHVGV PPAQAARSLA SFRNVKRRME VRGSVDGVTV YDDFAHHPTA IETTLAGLRA RVGRDNTRIL AVLEPRSNTM KLGVMKAQLP ASLADADLVF GYGAPTGRDA LGWNLGEALA PLGGKAQAFD NLDALVKAVV HTARPGDQIL VMSNGGFGGV HQKLLDALSA RGTS
NT Sequence
ATGCACATCC ACATCCTCGG TATTTGCGGC ACGTTCATGG GCGGTCTCGC GGTCCTCGCG CGCGGCGCGG GCCACACCGT GACGGGTTGC GACGCCGGCG TCTATCCGCC CATGAGCACG CAGCTGGAGG CGCAAGGCAT TGGTCTGATC GAAGGTTACG ACGCGGACCA GCTGAACGGT CTGAACGCGG ATCTGTTCGT GATCGGCAAC GTGGTCTCAC GCGGCAACCC GCTGATGGAG GCCATTCTCG ACCGCGGCCT GCCGTATACC TCCGGTCCGC AATGGCTCGG CGAGCATGTG CTGAACGGCA AATGGGTGCT GGCGGTGGCC GGCACGCACG GCAAGACGAC CACCAGCTCC ATGCTGACAT GGCTGCTCGA AGACGCCGGC CTCAATCCGG GCTTCCTGAT CGGCGGTGTG CCGCTGAATT TCGGCGTGTC GGCCCGGCTG ACCGATTCCA GCTTCTTCGT GATCGAAGCC GACGAATACG ACACGGCCTT TTTCGATAAG CGCTCGAAGT TCGTCCACTA CCGGCCCAAA ACCGCCGTGC TGAACAACCT CGAATTCGAT CACGCGGATA TTTTCCCCGA TCTGGCCGCG ATCGAAACTC AGTTCCACCA CCTGATCCGC ACCGTGCCGG GCATTGGCCG GATCGTGACG AACGGCCGTG AAGACGCGCT CGAGCGCGTG CTGACGCGCG GTTGCTGGAG CGAAGTGGAG CGCTTCGGCG TGCAGGGCGG CTGGGAAACA CTGCCGGCCG AAGACGGCGT GCCGGTCGAC GAGCGTTTCG CCGTCTATCA CAACAGTGAG CGCGTCGGCG CGGTCGAGTG GCAAGTGCAA GGCGAGCACA ACCGCATGAA CGCGCTGGCC GCGATCGCCG CCGCGCGCCA TGTCGGCGTG CCGCCGGCGC AAGCCGCCCG GTCGCTGGCG AGCTTTCGCA ACGTCAAGCG CCGCATGGAG GTGCGCGGCA GCGTCGACGG CGTCACCGTC TATGACGACT TCGCCCATCA TCCCACCGCC ATCGAGACCA CCCTGGCCGG GCTGCGCGCC CGCGTGGGCC GCGACAACAC GCGCATTCTC GCCGTACTGG AACCGCGCTC CAATACCATG AAGCTTGGTG TGATGAAAGC GCAGTTGCCG GCCAGCCTTG CCGACGCCGA TCTCGTCTTC GGCTACGGCG CGCCGACCGG CCGCGACGCG CTCGGCTGGA ATCTCGGCGA AGCGCTCGCG CCGCTCGGCG GCAAGGCGCA GGCTTTCGAC AATCTCGACG CGCTGGTCAA AGCGGTAGTT CACACAGCGC GCCCCGGCGA CCAGATTCTG GTGATGAGCA ACGGCGGCTT CGGCGGCGTG CATCAGAAAC TGCTCGATGC TCTTTCCGCG CGAGGCACTT CG
Details for BuxeA.00137.b.A2.GE33032
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 79
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAGVYPPMS TQLEAQGIGL IEGYDADQLN GLNADLFVIG NVVSRGNPLM EAILDRGLPY TSGPQWLGEH VLNGKWVLAV AGTHGKTTTS SMLTWLLEDA GLNPGFLIGG VPLNFGVSAR LTDSSFFVIE ADEYDTAFFD KRSKFVHYRP KTAVLNNLEF DHADIFPDLA AIETQFHHLI RTVPGIGRIV TNGREDALER VLTRGCWSEV ERFGVQGGWE TLPAEDGVPV DERFAVYHNS ERVGAVEWQV QGEHNRMNAL AAIAAARHVG VPPAXAARSL ASFRNVKRRM EVRGSVXGVT VYDDFAHHPT AXETTLAGLR AXXGXDXXXX XXVLEPRXXP
Validated NT Sequence
antttgnnnn nnttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg gcgtctatcc gcccatgagc acgcagctgg aggcgcaagg cattggtctg atcgaaggtt acgacgcgga ccagctgaac ggtctgaacg cggatctgtt cgtgatcggc aacgtggtct cacgcggcaa cccgctgatg gaggccattc tcgaccgcgg cctgccgtat acctccggtc cgcaatggct cggcgagcat gtgctgaacg gcaaatgggt gctggcggtg gccggcacgc acggcaagac gaccaccagc tccatgctga catggctgct cgaagacgcc ggcctcaatc cgggcttcct gatcggcggt gtgccgctga atttcggcgt gtcggcccgg ctgaccgatt ccagcttctt cgtgatcgaa gccgacgaat acgacacggc ctttttcgat aagcgctcga agttcgtcca ctaccggccc aaaaccgccg tgctgaacaa cctcgaattc gatcacgcgg atattttccc cgatctggcc gcgatcgaaa ctcagttcca ccacctgatc cgcaccgtgc cgggcattgg ccggatcgtg acgaacggcc gtgaagacgc gctcgagcgc gtgctgacgc gcggttgctg gagcgaagtg gagcgcttcg gcgtgcaggg cggctgggaa acactgccgg ccgaagacgg cgtgccggtc gacgagcgtt tcgccgtcta tcacaacagt gagcgcgtcg gcgcggtcga gtggcaagtg caaggcgagc acaaccgcat gaacgcgctg gccgcgatcg ccgccgcgcg ccatgtcggc gtgccgccgg cgcnagccgc ccggtcgctg gcgagctttc gcaacgtcaa gcgccgcatg gaggtgcgcg gcagcgtcna cggcgtcacc gtctatgacg acttcgccca tcatcccacc gccntcgaga ccaccctggc cgggctgcgc gcccncgngg gcngcgacna cncnngcatn nnnnnngtac tggaaccgcg ctncanacca tgaagctngg ngnnataaag nnnantnncn nnnnnnnttn nnannccnan nntngnnttn ngntagggnn nnncnannnn nnnnnnnnnn nnnnnnnngn nannnnngnn nnnnnnnngn nnnnnnnnnn nttnnnnnna nnnnnnnnnn nnnnggnnnn nnttt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGVYPPMS TQLEAQGIGL IEGYDADQLN GLNADLFVIG NVVSRGNPLM EAILDRGLPY TSGPQWLGEH VLNGKWVLAV AGTHGKTTTS SMLTWLLEDA GLNPGFLIGG VPLNFGVSAR LTDSSFFVIE ADEYDTAFFD KRSKFVHYRP KTAVLNNLEF DHADIFPDLA AIETQFHHLI RTVPGIGRIV TNGREDALER VLTRGCWSEV ERFGVQGGWE TLPAEDGVPV DERFAVYHNS ERVGAVEWQV QGEHNRMNAL AAIAAARHVG VPPAQAARSL ASFRNVKRRM EVRGSVDGVT VYDDFAHHPT AIETTLAGLR ARVGRDNTRI LAVLEPRSNT MKLGVMKAQL PASLADADLV FGYGAPTGRD ALGWNLGEAL APLGGKAQAF DNLDALVKAV VHTARPGDQI LVMSNGGFGG VHQKLLDALS ARGTS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccggcgtc tatccgccca tgagcacgca gctggaggcg caaggcattg gtctgatcga aggttacgac gcggaccagc tgaacggtct gaacgcggat ctgttcgtga tcggcaacgt ggtctcacgc ggcaacccgc tgatggaggc cattctcgac cgcggcctgc cgtatacctc cggtccgcaa tggctcggcg agcatgtgct gaacggcaaa tgggtgctgg cggtggccgg cacgcacggc aagacgacca ccagctccat gctgacatgg ctgctcgaag acgccggcct caatccgggc ttcctgatcg gcggtgtgcc gctgaatttc ggcgtgtcgg cccggctgac cgattccagc ttcttcgtga tcgaagccga cgaatacgac acggcctttt tcgataagcg ctcgaagttc gtccactacc ggcccaaaac cgccgtgctg aacaacctcg aattcgatca cgcggatatt ttccccgatc tggccgcgat cgaaactcag ttccaccacc tgatccgcac cgtgccgggc attggccgga tcgtgacgaa cggccgtgaa gacgcgctcg agcgcgtgct gacgcgcggt tgctggagcg aagtggagcg cttcggcgtg cagggcggct gggaaacact gccggccgaa gacggcgtgc cggtcgacga gcgtttcgcc gtctatcaca acagtgagcg cgtcggcgcg gtcgagtggc aagtgcaagg cgagcacaac cgcatgaacg cgctggccgc gatcgccgcc gcgcgccatg tcggcgtgcc gccggcgcaa gccgcccggt cgctggcgag ctttcgcaac gtcaagcgcc gcatggaggt gcgcggcagc gtcgacggcg tcaccgtcta tgacgacttc gcccatcatc ccaccgccat cgagaccacc ctggccgggc tgcgcgcccg cgtgggccgc gacaacacgc gcattctcgc cgtactggaa ccgcgctcca ataccatgaa gcttggtgtg atgaaagcgc agttgccggc cagccttgcc gacgccgatc tcgtcttcgg ctacggcgcg ccgaccggcc gcgacgcgct cggctggaat ctcggcgaag cgctcgcgcc gctcggcggc aaggcgcagg ctttcgacaa tctcgacgcg ctggtcaaag cggtagttca cacagcgcgc cccggcgacc agattctggt gatgagcaac ggcggcttcg gcggcgtgca tcagaaactg ctcgatgctc tttccgcgcg aggcacttcg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa