BuxeA.00137.a

UDP-N-acetylmuramate--L-alanine ligase

CENTER ID: BuxeA.00137.a
ORGANISM: Burkholderia xenovorans LB400
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00137.a.A1.GE33031 Full length( BuxeA.00137.a ) 1 467
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuxeA.00137.a.A1.PW33641 Full length( BuxeA.00137.a ) 1 467

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|266265.5.peg.4129
RefSeq: YP_560498.1
UniProt: Q13TZ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKHIVKHIHF VGIGGVGMSG IAEVLVNLGY QVSGSDLTSN AITDRLAALG ARIAIGHAAE NIEGANAVVV STAVRSDNPE VLAARHRRIP IVPRAVMLAE LMRLKQGIAI AGTHGKTTTT SLVASVLAAG GLDPTFVIGG RLISAGANAR LGTGDFIVAE ADESDASFLN LFPVIEVITN IDADHMDTYG HDFARLKQAF IEFTHRLPFY GIAVLCVDDP NVKEILPFVS KPIIRYGFAP DAQVRAVNVQ ARDGKMHFTA MREDAAPLDI VLNLPGEHNV QNALAAIAIA TELEVKDADI QRALADFNGV GRRFQRYGEV PVFSEGSTNG AYTLVDDYGH HPVEMAATVA AARGAFPGRR LVLAFQPHRF TRTRDCFEDF VKVLSTVDAL VLTEVYSAGE SPIVAADGRA LARALRVAGK VEPVFVDTVD EVPDALSAIV RDGDVVITMG AGSIGGVPAR LAQETKV
NT Sequence
ATGAAACACA TCGTCAAACA CATTCACTTT GTCGGCATCG GCGGTGTCGG CATGAGCGGC ATCGCGGAGG TGCTGGTCAA CCTCGGCTAT CAGGTGAGCG GCTCGGATCT GACGAGCAAC GCGATTACCG ATCGCCTCGC CGCACTCGGC GCGCGCATCG CGATCGGTCA CGCTGCGGAG AACATTGAAG GCGCGAACGC GGTAGTCGTT TCTACCGCGG TGCGTAGTGA CAATCCGGAA GTGCTGGCCG CGCGCCATCG CCGCATTCCG ATCGTGCCGC GTGCGGTGAT GCTCGCGGAA CTGATGCGCC TGAAGCAGGG CATTGCAATT GCCGGCACGC ACGGCAAGAC CACGACCACC TCGCTGGTGG CGAGCGTGCT CGCCGCCGGT GGACTCGACC CGACCTTCGT GATCGGCGGC CGGCTGATCA GCGCGGGCGC GAATGCGCGG CTCGGTACGG GCGACTTCAT CGTCGCGGAA GCCGACGAGT CGGACGCGTC GTTCCTGAAT CTGTTCCCGG TGATCGAAGT CATCACGAAC ATCGACGCCG ATCACATGGA CACCTACGGC CACGACTTCG CGCGGCTCAA GCAGGCGTTC ATCGAATTCA CGCACCGTCT GCCGTTCTAC GGCATCGCGG TGCTGTGCGT CGACGATCCG AACGTGAAGG AAATCCTGCC GTTCGTGTCG AAGCCGATCA TCCGCTACGG CTTCGCGCCG GACGCGCAGG TGCGCGCGGT GAACGTACAG GCACGCGACG GCAAGATGCA TTTTACGGCG ATGCGCGAAG ACGCGGCGCC GCTCGACATC GTGCTGAACC TGCCGGGCGA GCACAACGTG CAGAACGCTT TGGCCGCCAT TGCCATTGCG ACTGAACTTG AAGTGAAAGA TGCCGATATC CAGCGAGCGC TGGCGGATTT CAACGGCGTG GGCCGACGCT TCCAGCGCTA CGGCGAGGTG CCGGTGTTCA GCGAAGGCAG CACGAACGGC GCGTACACAC TGGTCGACGA CTACGGTCAT CACCCGGTCG AAATGGCGGC CACGGTGGCG GCGGCGCGCG GCGCATTTCC GGGCCGGCGT CTGGTGCTGG CATTCCAGCC GCACCGCTTC ACGCGTACGC GTGACTGCTT CGAAGATTTT GTGAAAGTGC TGTCGACCGT CGACGCGTTG GTGCTGACCG AAGTCTATTC GGCCGGCGAG TCGCCGATCG TCGCCGCGGA CGGCCGCGCT CTCGCGCGTG CGCTGCGGGT GGCAGGCAAG GTCGAACCGG TGTTTGTCGA TACGGTGGAT GAAGTGCCGG ACGCGCTCTC GGCGATAGTG CGCGACGGCG ATGTGGTGAT CACGATGGGC GCAGGTTCGA TCGGCGGTGT GCCGGCTCGC CTCGCGCAGG AAACAAAGGT G
Details for BuxeA.00137.a.A1.GE33031
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MKHIVKHIHF VGIGGVGMSG IAEVLVNLGY QVSGSDLTSN AITDRLAALG ARIAIGHAAE NIEGANAVVV STAVRSDNPE VLAARHRRIP IVPRAVMLAE LMRLKQGIAI AGTHGKTTTT SLVASVLAAG GLDPTFVIGG RLISAGANAR LGTGDFIVAE ADESDASFLN LFPVIEVITN IDADHMDTYG HDFARLKQAF IEFTHRLPFY GIAVLCVDDP NVKEILPFVS KPIIRYGFAP DAQVRAVNVQ ARDGKMHFTA MREDAAPLDI VLNLPGEHNV QNALAAIAIA TELEVKDADI QRALADFNGV GRRFQRYGEV PVFSEGSTNG AYTLVDDYGH HPVEMAATVA AARGAFPGRR LVLAFQPHRF TRTRDCFEDF VKVLSTVDAL VLTEVYSAGE SPIVAADGRA LARALRVAGK VEPVFVDTVD EXPDALSAIV RDGDVVITMG AGSIGGVPAR LAQET
Validated NT Sequence
ttgtttcctg cgcgaggcga gccggcacac cgccgatcga acctgcgccc atcgtgatca ccacatcgcc gtcgcgcact atcgccgaga gcgcgtccgg nncttcatcc accgtatcga caaacaccgg ttcgaccttg cctgccaccc gcagcgcacg cgcgagagcg cggccgtccg cggcgacgat cggcgactcg ccggccgaat agacttcggt cagcaccaac gcgtcgacgg tcgacagcac tttcacaaaa tcttcgaagc agtcacgcgt acgcgtgaag cggtgcggct ggaatgccag caccagacgc cggcccggaa atgcgccgcg cgccgccgcc accgtggccg ccatttcgac cgggtgatga ccgtagtcgt cgaccagtgt gtacgcgccg ttcgtgctgc cttcgctgaa caccggcacc tcgccgtagc gctggaagcg tcggcccacg ccgttgaaat ccgccagcgc tcgctggata tcggcatctt tcacttcaag ttcagtcgca atggcaatgg cggccaaagc gttctgcacg ttgtgctcgc ccggcaggtt cagcacgatg tcgagcggcg ccgcgtcttc gcgcatcgcc gtaaaatgca tcttgccgtc gcgtgcctgt acgttcaccg cgcgcacctg cgcgtccggc gcgaagccgt agcggatgat cggcttcgac acgaacggca ggatttcctt cacgttcgga tcgtcgacgc acagcaccgc gatgccgtag aacggcagac ggtgcgtgaa ttcgatgaac gcctgcttga gccgcgcgaa gtcgtggccg taggtgtcca tgtgatcggc gtcgatgttc gtgatgactt cgatcaccgg gaacagattc aggaacgacg cgtccgactc gtcggcttcc gcgacgatga agtcgcccgt accgagccgc gcattcgcgc ccgcgctgat cagccggccg ccgatcacga aggtcgggtc gagtccaccg gcggcgagca cgctcgccac cagcgaggtg gtcgtggtct tgccgtgcgt gccggcaatt gcaatgccct gcttcaggcg catcagttcc gcgagcatca ccgcacgcgg cacgatcgga atgcggcgat ggcgcgcggc cagcacttcc ggattgtcac tacgcaccgc ggtagaaacg actaccgcgt tcgcgccttc aatgttctcc gcagcgtgac cgatcgcgat gcgcgcgccg agtgcggcga ggcgatcggt aatcgcgttg ctcgtcagat ccgagccgct cacctgatag ccgaggttga ccagcacctc cgcgatgccg ctcatgccga caccgccgat gccgacaaag tgaatgtgtt tgacgatgtg tttcatcgaa ccaggaccct gggtctgagc ttccagggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKHIVKHIH FVGIGGVGMS GIAEVLVNLG YQVSGSDLTS NAITDRLAAL GARIAIGHAA ENIEGANAVV VSTAVRSDNP EVLAARHRRI PIVPRAVMLA ELMRLKQGIA IAGTHGKTTT TSLVASVLAA GGLDPTFVIG GRLISAGANA RLGTGDFIVA EADESDASFL NLFPVIEVIT NIDADHMDTY GHDFARLKQA FIEFTHRLPF YGIAVLCVDD PNVKEILPFV SKPIIRYGFA PDAQVRAVNV QARDGKMHFT AMREDAAPLD IVLNLPGEHN VQNALAAIAI ATELEVKDAD IQRALADFNG VGRRFQRYGE VPVFSEGSTN GAYTLVDDYG HHPVEMAATV AAARGAFPGR RLVLAFQPHR FTRTRDCFED FVKVLSTVDA LVLTEVYSAG ESPIVAADGR ALARALRVAG KVEPVFVDTV DEVPDALSAI VRDGDVVITM GAGSIGGVPA RLAQETKV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaacacatc gtcaaacaca ttcactttgt cggcatcggc ggtgtcggca tgagcggcat cgcggaggtg ctggtcaacc tcggctatca ggtgagcggc tcggatctga cgagcaacgc gattaccgat cgcctcgccg cactcggcgc gcgcatcgcg atcggtcacg ctgcggagaa cattgaaggc gcgaacgcgg tagtcgtttc taccgcggtg cgtagtgaca atccggaagt gctggccgcg cgccatcgcc gcattccgat cgtgccgcgt gcggtgatgc tcgcggaact gatgcgcctg aagcagggca ttgcaattgc cggcacgcac ggcaagacca cgaccacctc gctggtggcg agcgtgctcg ccgccggtgg actcgacccg accttcgtga tcggcggccg gctgatcagc gcgggcgcga atgcgcggct cggtacgggc gacttcatcg tcgcggaagc cgacgagtcg gacgcgtcgt tcctgaatct gttcccggtg atcgaagtca tcacgaacat cgacgccgat cacatggaca cctacggcca cgacttcgcg cggctcaagc aggcgttcat cgaattcacg caccgtctgc cgttctacgg catcgcggtg ctgtgcgtcg acgatccgaa cgtgaaggaa atcctgccgt tcgtgtcgaa gccgatcatc cgctacggct tcgcgccgga cgcgcaggtg cgcgcggtga acgtacaggc acgcgacggc aagatgcatt ttacggcgat gcgcgaagac gcggcgccgc tcgacatcgt gctgaacctg ccgggcgagc acaacgtgca gaacgctttg gccgccattg ccattgcgac tgaacttgaa gtgaaagatg ccgatatcca gcgagcgctg gcggatttca acggcgtggg ccgacgcttc cagcgctacg gcgaggtgcc ggtgttcagc gaaggcagca cgaacggcgc gtacacactg gtcgacgact acggtcatca cccggtcgaa atggcggcca cggtggcggc ggcgcgcggc gcatttccgg gccggcgtct ggtgctggca ttccagccgc accgcttcac gcgtacgcgt gactgcttcg aagattttgt gaaagtgctg tcgaccgtcg acgcgttggt gctgaccgaa gtctattcgg ccggcgagtc gccgatcgtc gccgcggacg gccgcgctct cgcgcgtgcg ctgcgggtgg caggcaaggt cgaaccggtg tttgtcgata cggtggatga agtgccggac gcgctctcgg cgatagtgcg cgacggcgat gtggtgatca cgatgggcgc aggttcgatc ggcggtgtgc cggctcgcct cgcgcaggaa acaaaggtgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BuxeA.00137.a.A1.PW33641
PURIFICATION DATe: 11/19/2011
CONCENTRATION: 30.43mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MKHIVKHIHF VGIGGVGMSG IAEVLVNLGY QVSGSDLTSN AITDRLAALG ARIAIGHAAE NIEGANAVVV STAVRSDNPE VLAARHRRIP IVPRAVMLAE LMRLKQGIAI AGTHGKTTTT SLVASVLAAG GLDPTFVIGG RLISAGANAR LGTGDFIVAE ADESDASFLN LFPVIEVITN IDADHMDTYG HDFARLKQAF IEFTHRLPFY GIAVLCVDDP NVKEILPFVS KPIIRYGFAP DAQVRAVNVQ ARDGKMHFTA MREDAAPLDI VLNLPGEHNV QNALAAIAIA TELEVKDADI QRALADFNGV GRRFQRYGEV PVFSEGSTNG AYTLVDDYGH HPVEMAATVA AARGAFPGRR LVLAFQPHRF TRTRDCFEDF VKVLSTVDAL VLTEVYSAGE SPIVAADGRA LARALRVAGK VEPVFVDTVD EXPDALSAIV RDGDVVITMG AGSIGGVPAR LAQET
Validated NT Sequence
ttgtttcctg cgcgaggcga gccggcacac cgccgatcga acctgcgccc atcgtgatca ccacatcgcc gtcgcgcact atcgccgaga gcgcgtccgg nncttcatcc accgtatcga caaacaccgg ttcgaccttg cctgccaccc gcagcgcacg cgcgagagcg cggccgtccg cggcgacgat cggcgactcg ccggccgaat agacttcggt cagcaccaac gcgtcgacgg tcgacagcac tttcacaaaa tcttcgaagc agtcacgcgt acgcgtgaag cggtgcggct ggaatgccag caccagacgc cggcccggaa atgcgccgcg cgccgccgcc accgtggccg ccatttcgac cgggtgatga ccgtagtcgt cgaccagtgt gtacgcgccg ttcgtgctgc cttcgctgaa caccggcacc tcgccgtagc gctggaagcg tcggcccacg ccgttgaaat ccgccagcgc tcgctggata tcggcatctt tcacttcaag ttcagtcgca atggcaatgg cggccaaagc gttctgcacg ttgtgctcgc ccggcaggtt cagcacgatg tcgagcggcg ccgcgtcttc gcgcatcgcc gtaaaatgca tcttgccgtc gcgtgcctgt acgttcaccg cgcgcacctg cgcgtccggc gcgaagccgt agcggatgat cggcttcgac acgaacggca ggatttcctt cacgttcgga tcgtcgacgc acagcaccgc gatgccgtag aacggcagac ggtgcgtgaa ttcgatgaac gcctgcttga gccgcgcgaa gtcgtggccg taggtgtcca tgtgatcggc gtcgatgttc gtgatgactt cgatcaccgg gaacagattc aggaacgacg cgtccgactc gtcggcttcc gcgacgatga agtcgcccgt accgagccgc gcattcgcgc ccgcgctgat cagccggccg ccgatcacga aggtcgggtc gagtccaccg gcggcgagca cgctcgccac cagcgaggtg gtcgtggtct tgccgtgcgt gccggcaatt gcaatgccct gcttcaggcg catcagttcc gcgagcatca ccgcacgcgg cacgatcgga atgcggcgat ggcgcgcggc cagcacttcc ggattgtcac tacgcaccgc ggtagaaacg actaccgcgt tcgcgccttc aatgttctcc gcagcgtgac cgatcgcgat gcgcgcgccg agtgcggcga ggcgatcggt aatcgcgttg ctcgtcagat ccgagccgct cacctgatag ccgaggttga ccagcacctc cgcgatgccg ctcatgccga caccgccgat gccgacaaag tgaatgtgtt tgacgatgtg tttcatcgaa ccaggaccct gggtctgagc ttccagggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKHIVKHIH FVGIGGVGMS GIAEVLVNLG YQVSGSDLTS NAITDRLAAL GARIAIGHAA ENIEGANAVV VSTAVRSDNP EVLAARHRRI PIVPRAVMLA ELMRLKQGIA IAGTHGKTTT TSLVASVLAA GGLDPTFVIG GRLISAGANA RLGTGDFIVA EADESDASFL NLFPVIEVIT NIDADHMDTY GHDFARLKQA FIEFTHRLPF YGIAVLCVDD PNVKEILPFV SKPIIRYGFA PDAQVRAVNV QARDGKMHFT AMREDAAPLD IVLNLPGEHN VQNALAAIAI ATELEVKDAD IQRALADFNG VGRRFQRYGE VPVFSEGSTN GAYTLVDDYG HHPVEMAATV AAARGAFPGR RLVLAFQPHR FTRTRDCFED FVKVLSTVDA LVLTEVYSAG ESPIVAADGR ALARALRVAG KVEPVFVDTV DEVPDALSAI VRDGDVVITM GAGSIGGVPA RLAQETKV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAACACATC GTCAAACACA TTCACTTTGT CGGCATCGGC GGTGTCGGCA TGAGCGGCAT CGCGGAGGTG CTGGTCAACC TCGGCTATCA GGTGAGCGGC TCGGATCTGA CGAGCAACGC GATTACCGAT CGCCTCGCCG CACTCGGCGC GCGCATCGCG ATCGGTCACG CTGCGGAGAA CATTGAAGGC GCGAACGCGG TAGTCGTTTC TACCGCGGTG CGTAGTGACA ATCCGGAAGT GCTGGCCGCG CGCCATCGCC GCATTCCGAT CGTGCCGCGT GCGGTGATGC TCGCGGAACT GATGCGCCTG AAGCAGGGCA TTGCAATTGC CGGCACGCAC GGCAAGACCA CGACCACCTC GCTGGTGGCG AGCGTGCTCG CCGCCGGTGG ACTCGACCCG ACCTTCGTGA TCGGCGGCCG GCTGATCAGC GCGGGCGCGA ATGCGCGGCT CGGTACGGGC GACTTCATCG TCGCGGAAGC CGACGAGTCG GACGCGTCGT TCCTGAATCT GTTCCCGGTG ATCGAAGTCA TCACGAACAT CGACGCCGAT CACATGGACA CCTACGGCCA CGACTTCGCG CGGCTCAAGC AGGCGTTCAT CGAATTCACG CACCGTCTGC CGTTCTACGG CATCGCGGTG CTGTGCGTCG ACGATCCGAA CGTGAAGGAA ATCCTGCCGT TCGTGTCGAA GCCGATCATC CGCTACGGCT TCGCGCCGGA CGCGCAGGTG CGCGCGGTGA ACGTACAGGC ACGCGACGGC AAGATGCATT TTACGGCGAT GCGCGAAGAC GCGGCGCCGC TCGACATCGT GCTGAACCTG CCGGGCGAGC ACAACGTGCA GAACGCTTTG GCCGCCATTG CCATTGCGAC TGAACTTGAA GTGAAAGATG CCGATATCCA GCGAGCGCTG GCGGATTTCA ACGGCGTGGG CCGACGCTTC CAGCGCTACG GCGAGGTGCC GGTGTTCAGC GAAGGCAGCA CGAACGGCGC GTACACACTG GTCGACGACT ACGGTCATCA CCCGGTCGAA ATGGCGGCCA CGGTGGCGGC GGCGCGCGGC GCATTTCCGG GCCGGCGTCT GGTGCTGGCA TTCCAGCCGC ACCGCTTCAC GCGTACGCGT GACTGCTTCG AAGATTTTGT GAAAGTGCTG TCGACCGTCG ACGCGTTGGT GCTGACCGAA GTCTATTCGG CCGGCGAGTC GCCGATCGTC GCCGCGGACG GCCGCGCTCT CGCGCGTGCG CTGCGGGTGG CAGGCAAGGT CGAACCGGTG TTTGTCGATA CGGTGGATGA AGTGCCGGAC GCGCTCTCGG CGATAGTGCG CGACGGCGAT GTGGTGATCA CGATGGGCGC AGGTTCGATC GGCGGTGTGC CGGCTCGCCT CGCGCAGGAA ACAAAGGTGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA